(in English): The thesis focuses on the Czech sugar factory in Gorna Oryahovitsa in northern Bulgaria, especially on the workers' colony which existed between 1912 and 1948 when the company was being put under state control. The paper provides a comprehensive report on the circumstances of the origin, development and current condition of the sugar factory and the colony. The study puts the specific instance of the Czech minority in Gorna Oryahovitsa into a wider context of the Czech migration into Bulgaria. It also provides a comprehensive picture of the Czech colony and its inhabitants, bringing new insights into their lifestyle, activities and contacts with the major society. The core of this work is to use written and pictorial materials. Therefore, the basic methodological approach was to search, collect and record the materials that in some way affected the Czech sugar factory and the colony, both in Bohemia and Bulgaria. These materials were first analyzed and then organized to create meaningful frameworks for subsequent interpretation.

The yeast Saccharomyces cerevisiae when growing on solid medium forms structured colonies. During its development, two subpopulations of cells are formed, termed as U cells ("upper cells") and L cells ("lower cells"). This nomenclature derives from their position within the colony. These cells differ from each other considering their morphology, metabolism, physiology and are characterized by varying degrees of resistance to stress. This diploma thesis deals with new methodological approaches that can be used in further research of yeast differentiation. The first part deals with yeast differentiation and de-differentiation. In the experimental part, the development of mechanically mixed yeast colonies was monitored. The development was monitored in situ in the colony section using fluorescence and "wide-field" microscopy. The ability to de-differentiate of already specialized cells was evaluated mainly according to the nature of the expression of the fluorescently labeled protein Ato1p, which serves as a protein marker of U cells. The second part deals with the optogenetic system EL222 and its possible use in yeast. Many optogenetic tools have been described to control various cellular processes using light. One of these optogenetic tools is the EL222 system, which allows the induction of...

The diploma thesis "The future of human exoplanet settlement from the present point of view" is focused on the analysis of the development of the urban compo-sition of the city for the purpose of finding the most suitable concept of the set-tlement. Its foundation is hypothetically put on some of the planets outside the solar system, where could be comparable conditions as on Earth. Installation in these extreme conditions is chosen deliberately so that the essential aspects of the urban layout come to the fore. In this way it is possible at the same time to verify the presumption of stability of the basic assumptions of a functional city and ap-ply the final conclusions in the current conditions as well.

Yeasts are unicellular eukaryotic microorganisms, capable of forming of organised multicellular communities, the colonies. Many yeast strains possess a characteristic colony morphology under defined living conditions. Another feature typical for many feral and pathogenic yeast strains is the ability to switch their morphotype. This phenomenon, called the phenotypic switching, contributes to a rapid adaptation to the changing harmful environment and is often connected with changes of the stress resistance or with the changes of virulence of pathogenic yeasts. Phenotypic switching can be observed even in non-pathogenic yeast Saccharomyces cerevisiae. The strain BR-F, isolated from nature, switches under laboratory conditions from fluffy to smooth morphology of the strain BR-S. This phenotypic switch is accompanied by broad changes in the phenotype. Transcriptome analyses of the strains BR-F and BR-S have shown, among others, changes in expression of the subtelomeric genes that are under control of the histone acetylases and deacetylases. My work was aimed to the histone deacetylase Sir2p, which could influence the phenotypic switching in Saccharomyces cerevisiae. The sir2 deletion mutant of the strain BR-S, prepared in our laboratory, was used for my studies. The results show, that the strain BR-S...

In the yeast Saccharomyces cerevisiae, stress on the cell wall is caused by various external influences (e.g. exposure to chemicals, oxidative stress, osmotic changes, pH changes or heat shock) which trigger the cell wall integrity signalling pathway (CWI). The aim of my work was to investigate the effect of the CWI pathway on yeast colony morphogenesis. Using strains with deletions in genes of the CWI pathway derived from two parental strains BR-F-Flo11p-GFP and PORT, I have found that differences in genetic background influences the process and activation of this pathway. Among the strains derived from BR-F-Flo11p-GFP, only the strain with the deletion of MID2 affects the appearance of colonies. MID2 encodes a cell-surface sensor of CWI pathway. In all deletion strains derived from PORT, the disruption of the CWI pathway causes a slower development of colonies growing on glycerol medium supplemented with 0,05 mM selenate inducing fluffy colony morphology. The largest effect has deletion of gene MTL1 which also encodes a cell-surface sensor with homology to Mid2. I have confirmed that strains with deletions in genes of CWI pathway have altered sensitivity to inhibitors disrupting cell wall integrity (Calcofluor white, Congo red, zymolyase). By means of zymolyase assay, I have confirmed the...

Saccharomyces cerevisiae strains isolated from the wild are able to exhibit multicellular social behaviour and to form complex structured colonies resembling in many properties highly resistant biofilms of pathogenic yeasts. The capability of phenotypic variability, i.e. high frequency transition between two or more different phenotypes, is another feature typical for the wild yeast strains. Such phenotypic changes are in case of pathogenic yeast often connected with changes in virulence and resistance to stress and antifungal treatment. Long-term cultivation of the wild yeast strains under laboratory conditions leads to their domestication, i.e. transition to smooth colonies and loss of some features typical for structured colonies. This process is, similarly to phenotypic switching, accompanied by significant changes in gene expression and global change of colony lifestyle. Mechanisms underlying yeast phenotypic transitions are ascribed to epigenetic regulation of gene expression via transcriptional silencing conferred by histone deacetylases. This work deals with the study of such mechanisms using knock-outs of selected genes with putative function in formation of structured colonies in wild and domesticated strains. The achieved results show, that NAD+-dependent histone deacetylase Sir2p influences...

Previous research of colonies formed by yeast Saccharomyces cerevisiae growing on glycerol agar medium revealed two major cell types of U and L cells that are formed within these colonies. This colonial cell differentiation seem to be caused by communication among yeast cells as well as whole colonies and affected by changes in the environment (for example changes in nutrients). Studies of U and L cells showed that U cells are more resistant against biological, chemical and physical stresses than L cells. The aim of this thesis was to isolate U and L cell types and investigate their resistance against selected low molecular weight chemical substances produced in Ehrlich pathway. Ehrlich pathway was discovered in 1907 and was classified as amino acid catabolic pathway in yeast S. cerevisiae. The low molecular intermediates are formed in Ehrlich pathway which are called fusel (original name from German) alcohols and acids. These chemical substances are widely used in food industry and cosmetics especially because of their aroma. Several studies provided indications that these chemical substances may affect development of colonies and important yeast functions such as switching to the pseudohypfal growth of S. cerevisiae cells. Some chemical substances of the Ehrlich pathway were selected and their effects on...

The master thesis investigates the care of orphans and abandoned children paying special attention to how the care was executed in Prague in the 1st half of the 20th century. First, the thesis describes the general development of care of these children and of legislation. It then focuses on Prague social care of children. The orphans and abandoned children could be provided either in foster families (this form of care was more frequent) or in orphanages. Prague children were often placed outside of Prague to the so called family colonies, where the foster families were concentrated and where it was easier to control the quality of care those children attained in foster families. The other option was care in orphanages. Those could be established by the city, by the church or by an independent charity association. For each option I chose one Prague orphanage as an example. I also tried to describe the everyday life in these institutions. The last chapter focuses on the period view on advantages and disadvantages of both forms of care. Keywords: orphans, abandoned children, social care, Prague, children's homes, foster care, family colonies

The diploma work deals with the paintings of Antonín Hudeček from around 1900, which are viewed from two positions. The first thematic area includes the analysis of selected thought sources (the topic of a soul or a mood in the art), the motives (mirroring) and the themes (bathing boys) of Hudeček's paintings, which are treated with regard to the period art criticism a thought currents. The second part is made by analysis of some art sources which were essential for Antonín Hudeček's paintings around 1900. These were especially Hudeček's training with figuralists in Prague and Munich and the possible foreign inspiration, most importantly from the context of the contemporary art colonies and informal art groups. Fundamental theme of the work is the relationship of figure and landscape in the work of Antonín Hudeček around 1900. The catalogue of Hudeček's paintings from this period and excerpts from selected periodicals serve as the main methodological tool for comparison of Hudeček's work with other artists. Hudeček's schematic biography is also presented in the appendix of the work.

This construction programme deals with a hermit colony located in the western part of the Vysočina Region. This hermit colony has been established to provide accommodation for women living in this area permanently while offering an opportunity of occasional relaxation for people who are living hectic lives in urban environment. The aim of my project is to design the remaining parts of the colony, i.e. two hermitages and the community’s chapel. A hermitage is a one-storey building intended for one person only. The load-bearing walls of the individual hermitages are designed to be built from different materials (wooden and masonry constructions) whereas the elevational structures of all the buildings are to be the same. The chapel’s wall system combines stone and wood. The chapel will serve solely the purposes of the community. The main objective of my project is to respect and maintain the natural features of the landscape to the maximum extent possible.