The diploma thesis deals with the influence of technological parameters on the emulsion copolymerization of methyl methacrylate with n butyl acrylate. The theoretical part contains knowledge in the field of influences of proces tempereture, concentration of initiator, concentration of emulsifier, stirring rate and dose rate on emulsion polymerization. In the experimental part was observed effect of the concentration of the ionic emulsifier, nonionic emulsifier, furthemore was observed effect of stirring speed and the concentration of initiator K2S2O8 on conversion of copolymerization. During all copolymerizations, conversions was determinated via solids content evaluation. By dynamic light scattering was determinated and presented polymer particle size of the prepared emulsions. Stability of emuldions was observed via effect of different ionic strenght and yield of freeze thaw cycles coalescence. Based on experimental results were proposed conditions of polyacrylic lattex preparation.

Cell cultures are grown in special incubators ensuring appropriate culture conditions for cell survival. The introduction of the diploma thesis is dedicated to the cultivation of cells in vitro and description of sensors, especially designated for the sensing of gas concentration. The main essence of this work is a project and realization of the monoclimatic incubation chamber for the incubation of cell cultures with the opportunity to observe them under a confocal microscope. Another part of this work is dealing with the project and application of autonomous sensing system and regulation of gas concentration (O2 and CO2) in the chamber. The nal part of thesis is dedicated to the presentation of the project correctness verication of the incubation chamber.

The aim of this master thesis is a research of effects of clay plaster application and natural ventilation on heat storage of a room. In addition to theoretical analysis of basic characteristics of clay as material used in the field of civil engineering, an experimental verification of these characteristical features is carried out as well. The thesis also studies thermal comfort of a room in terms of humidity and concentration of CO2 in dependence of natural ventilation. In the last part, numerical verification of effects of clay plaster application on heat storage is calculated using Simulace 2015 software.

The thesis deals with the issue of indoor air quality of schools and kindergartens. It focuses particularly on the inadequate ventilation and the application of forced ventila-tion in these types of buildings. The proposed solution is applied to a kindergarten in Kuřim. Measurements of the C02 concentration levels were performed in the presence and absence of air conditioning. To elaborate the proposed solution a simulation was made using a software called ANSYS Fluent. Input values for the simulation were obtained from an experimental measurement of a ventilation diffuser made by a company called Climecon ROX using the PIV method. This diffuser was then installed in the kindergarten. The measure-ments were carried out in the framework of the project the experimental validation of numerical models of the air flow in buildings marked with FAST-S-6-3387.

Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Consultant: PharmDr. Jan Marek, Ph.D. Supervisor: doc. PharmDr. Veronika Nováková, Ph.D. Student: Kateřina Pekařová Title of Thesis: Cationic surface active compounds and evaluation of its properties Quaternary ammonium salts are used as surfactants in many branches of the food, pharmaceutical and chemical industries. The basic properties of these substances were studied in the thesis. The critical micellar concentration of the homologous series of five substances was determined using the conductometric method. The theory of declining critical micellar concentration with the increasing length of the side alkyl chain has been verified. The hydrolytic activity of two commonly used cationic surfactants benzalconium chloride and benzoxonium chloride for the decomposition of the organophosphorus pesticide fenitrothion was tested. For these two substances, the effect of pH and temperature changes was evaluated. The influence of tenside concentration was also monitored. Benzoxonium chloride at 50 řC and pH 11was found as the most effective hydrolytic catalyst for the decomposition of the organophosphorus pesticide fenitrothione.

This thesis consists of five essays dealing with the fee and commission income in European banks from the macro, sector and bank level perspectives. This topic is of high relevance since fee income represents the largest part of the non-interest income of EU banks. The first part of the thesis deals with banks in general terms, while the second part is focused solely on cooperative banks, which represent approximately 20% of the EU banking sector. We compare the magnitude of the bank fee income across EU countries and study its determinants and impacts on banks' performance. The first essay compares the magnitude of the fee income across EU countries with a special emphasis on the Czech Republic from a macroeconomic perspective. First, we conclude that Czech banks are not abnormally dependent on fee income and their outstanding performance can be attributed to sound risk management, high liquidity and sufficient capital buffers rather than to excessive fees. Second, our evidence suggests that the share of fee income had an increasing trend in EU countries in recent years, which might be connected to the effort to maintain sufficient profitability in the low interest rates environment. We also discuss how new entrants, the so called low-cost banks, changed the banking sector in the Czech Republic....

4 ABSTRACT The aim of this thesis is to draw attention to the process of concentration in tourism.Various locations within the monitored area have different potential for tourism. This aspect leads to a concentration of tourists and services to certain centers. In the area of interest there are two attractive location for a tourist city of Český Krumlov and Lipno dam. These two sites are the frequently visited places, both in southern Bohemia and in the whole Czech Republic. However, the area has a hide potential for the development of alternative forms od tourism. The intent of this thesis is based on the available data and information to evaluate the process of concentration of tourism in the case of Český Krumlov district and propose appropriate tools to deconcentration of tourism and the use of peripheral sites. Keywords: concentration of tourism, alternative forms of tourism, Český Krumlov, Lipno dam

Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Autor: Bc. Marcela Vízková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title: In vitro screening of novel potentially active antibacterial compounds III Since the discovery of penicillin, antibiotics have become part of modern therapeutic approaches. But the global spread of resistance makes their future uncertain. It is necessary to find new antibacterial substances useful in practice. As resistance is a global threat, the theoretical part deals with this issue. In addition to resistance, the theoretical part also briefly describes the antibiotics used, including new antibiotics, which were recently registered or likely to be registered. For selected groups, their mechanism of action is described in detail. Sensitivity should be determined to correctly indicate the antibiotic treatment. For this reason, the theoretical part also describes methods by which the sensitivity of a microbe to an antibiotic can be determined qualitatively or quantitatively. The theoretical part does not neglect the history of the development of antibacterial substances and familiarization with antibiotic policy in the Czech Republic. This diploma thesis is based on...

Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Specialist in Laboratory Methods Candidate: Bc. Aneta Janků Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of novel potentially active antimycotic compounds III Key words: yeasts, moulds, antimycotics, mechanism of action of polyenes, microdilution broth method, MIC - minimal inhibition concentration. The main topic of this diploma thesis is in vitro screening of new, potentially active antifungal compounds. The introductory chapter in the theoretical part of the work is devoted to the epidemiology of fungal infections. The classification of micromycetes and the characteristics of the fungal eukaryotic cell are given below, focusing on composition of the plasma membrane and the cell wall. A separate chapter also deals with specific species of fungi and the diseases they cause. The main topic is drugs from the group of antifungals, where more attention is focused on polyene antifungals, their mechanism of action in detail and the emergence of resistance. An integral part are also the stages of development of new drugs and a list of novels developing antifungals. The final chapter describes the types of methods designed for in vitro evaluation...

Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Bc. Tereza Pchálková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of potential antimycobacterial compounds active against fast growing strains of Mycobacterium genus II Key words: Mycobacteria, Tuberculosis, Antituberculotics, Microdilution broth method, Minimum inhibition concentration Background: The aim of this diploma thesis has been screening of in vitro antimycobacterial activity of novel compounds against fast growing strains of the genus Mycobacterium (Mycobacterium smegmatis and Mycobacterium aurum). Another aim has been predicting the structure-activity relationships for tested compounds. Methods: The technique used for activity determination was microdilution broth method. The value of minimum inhibition concentration for each compound was determined. The evaluation was performed visually and spectrophotometrically using the Alamar blue indicator. Results: A total of 79 compounds were tested. 22 tested compounds showed significant activity against mycobacteria. The substances were sorted according to the similarity in chemical structure into 10 groups. From a chemical point of view, these...