This thesis studied the influence of elevated carbon dioxide concentration on the properties of ears, grain and flour in two varieties of wheat (Triticum aestivum). The Vánek and Septima varieties were grown in special greenhouses, both in normal atmosphere with carbon dioxide concentration (AC = 385 ?mol?mol-1), and at elevated carbon dioxide concentration (EC = 700 ?mol?mol-1). Quantitative analysis of grain (the content of crude protein, starch, moisture and hardness of grain) was carried out using NIR, gluten content (gluten index) was determined after washing with water and gluten quality was expressed as falling number. Total crude protein content was determined according to Kjeldahl, total amino acids and amino acids content by ion exchange chromatography. Variety Vánek showed significantly larger size of the spikes, more grains and higher grain weight, when it was cultivated in the atmosphere of elevated CO2 concentration. For variety Septima, the size of AC and EC ear variants was comparable, but the ears of corn grown in the EC had a significantly greater number of grains and greater weight. Vánek variety grown in elevated CO2 concentration had lower weight of 100 grains, Septima variety grown in elevated CO2 concentration had greater weight of 100 grains. The varieties Vánek and Septima grown at elevated CO2 concentration showed significantly lower content of crude protein (28.4%) and gluten index (by 34.6%). In comparison with the AC variant, EC also showed lower overall content of crude protein and amino acids.

This work deals with issues of detection chlorine, measurement pH, atmospheric pressure and temperature. The general aim is to study of the problem, the choice appropriate sensors and methods for detection, create schematic diagram and implementation of the analyzer. An integral part of wireless communication with the computer, susceptible the storage of date and makes it possible to further evalution.

The aim of this work was focused on the determination of pesticides concentration in water solution. Pesticides are indissoluble in aqueous solutions and impedance or voltametric/amperometric response of the pure water and water with pesticides is identical. Therefore was necessary find a way how rapidly, effectively and economically measure the pesticides concentration. One way is measurement of conductivity with electrochemical microsensors after enzymatic reaction.

The present study investigates effects of elevated atmospheric carbon dioxide concentration, different nitrogen fertilization levels, drought and UV radiation on protein content of wheat gluten fractions glutenins and gliadins. Winter wheat cultivar Bohemia was grown under ambient CO2 concentration (AC; 400 mol CO2.mol-1) and elevated CO2 concentration (EC; 700 mol CO2.mol-1). Half of the samples was fertilized with 200 kg N.ha-1 (N+) and the other part stayed unfertilized (N–). Other environmental factors were UV radiation exclusion (UV–, UV+) and drought (DRY, WET). Gliadins were separated by A-PAGE, glutenins by SDS-PAGE. Proteins were quantified by computer densitometry. Nitrogen fertilization caused an significat increase of gliadins and glutenins. While some gliadins subfractions were significantly lowered by drought, HMW glutenin subunits showed significant increase. UV radiation exclusion resulted in significant decrease of some gliadin subfractions and glutenin subunits. CO2 enrichment caused significant increase of glutenin subfractions HMW 1 and 2, while gliadin subfractions -5 1 and 1 were significantly decreased by elevated CO2 concentration.

Presented diploma thesis deals with drug encapsulation of Sulfathiazole (STA) in thermosensitive hydrogel composed of amphiphilic fibrous molecules of copolymer poly(D,L-lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA). In the experimental part, the structure of the micelles which is dependent on the concentration of the default copolymer, was thoroughly described. The method of dynamic light scattering (DLS) was used to dimensionally characterize the micellar size in aqueous solution. At the same time, the Cryo-Transmission Electron Microscopy (Cryo-TEM) imaging technique confirmed the transition from spherical micelles to fibrous branched structures with a number of topological interactions. The change in viscoelastic properties of micellar hydrogel after addition of STA was studied by rheological analysis. The studied triblock copolymer appears to be a suitable tissue engineering material as a carrier in applications for targeted drug delivery and tissue regeneration.

Hydrophilic interaction liquid chromatography is a frequently used separation method for analysis of polar compounds. It is an alternative method to reversed-phase chromatography, where these compounds show insufficient or very weak retention. A high number of stationary phases are currently available for HILIC and new ones are still being developed. The aim of this diploma thesis was to characterize and compare three relatively new HILIC columns containing an unmodified silica gel (HILIC-A), aminopropyl modified silica (HILIC-B) and polyhydroxyl chain modified silica (HILIC-N) as stationary phase. Based on the study of the effect of acetonitrile content in mobile phase on the retention of a model set of peptides, a multimodal retention mechanism was demonstrated. Analysis of 18 model analytes with different pKa values showed, that the composition of aqueous part of mobile phase (buffer), more specifically it's concentration and pH value, has a significant impact on retention of ionized analytes and peptides on the studied stationary phases. A significant contribution of ionic interactions to retention was observed on HILIC-B and HILIC-A columns. The retention of basic compounds on the HILIC-B column increased with increasing ionic strength, while it decreased on the HILIC-A column. Increasing the...

The thesis is focused on the brewing industry in the Czech Republic and changes within it which influenced the development of small Czech breweries. An analysis of the industry is performed using the Porter's Five Forces model focusing on the five competitive forces represented by the threat of new entrants, the bargaining power of suppliers, the bargaining power of customers, substitute products or services, and established rivals. Market concentration is also analyzed given the changes in the brewing industry. Changes in consumer demand and their influence on the brewing industry are examined subsequently. In particular, the change of consumer preferences leading to an increased consumption of stronger beers or aspects of beer selection are analyzed. Based on the findings, the impacts of these changes on small Czech breweries are described. In the next part of the thesis, economic results of selected breweries are compared using the indicators of value added margins, return on assets, return on equity and return on sales.

The aim of the thesis "Evaluation of qualitative parameters of rams ejaculates" was to compare techniques of ejaculate collection of rams of combined breed. The sampling technique for the artificial vagina was compared to that of the electro-ejaculator. The quantitative and qualitative indicators of ejaculates of rams were evaluated in the thesis. The parameters evaluated were: ejaculate volume, sperm movement, sperm initial aktivity, sperm concentration in ejaculate and sperm morphology. Samples were taken at four days. A total of 24 samples were obtained from 10 rams of the combined breed, of which 12 were sampled by artificial vagina sampling by stimulation with fixed sheep and 12 samples by electro-ejaculator. The electro-ejaculator collection technique was found to have lower sperm concentration (P≤0.01) and lower initial activity (P≤0.05) compared to the artificial vagina collection technique. The difference in ejaculate volume and sperm movement in the compared methods was not statistically demonstrated. No significant differences between sperm collection techniques in sperm abnormalities were observed or statistically demonstrated. The results of the diploma thesis showed that the better technique of ejaculate collection is taking on the artificial vagina, because it leads to higher initial activity of sperm (P≤0.05) and higher concentration of sperm (P≤0.01), which can be used mainly in the preparation of insemination doses.

The thesis describes the effects of physical cash pooling implementation in a multinational company. The aim is to establish a general methodological framework for evaluating the costs and benefits of physical cash concentration. The basis is a theoretical overview of the cash concentration, which includes the definition of the main concepts and the methodical procedure for the implementation of this liquidity management tool in the company. In the empirical part there is a concrete application of the methodical procedure, internal and external environment analysis, as well as discussion of the effects of cash pooling implementation. The final chapter sets out a general assessment framework for the costs and benefits of physical cash pooling.

This thesis deals with both theoretical and practical aspects of banking fee and commission income in the European Union. Since fee income represents the largest part of non-interest income earned by banks, it remains a major challenge for bank management to set and maintain an appropriate fee policy. Nevertheless, solving for the optimal fee structure has not yet been accomplished either on a theoretical level, or in actual practice. In the thesis, we analyse fee income in EU banking sectors. Our results show that the Czech banking sector was not abnormally dependent on fee income compared to other EU countries in the period 2007-2012. As a result, we argue that the high profitability of Czech banks cannot be attributed to abnormal banking fees and commission income, but rather other factors should be considered. Moreover, we study the determinants of fee income share in individual banks and discuss the impact of market concentration on the magnitude of banking fees. We conclude that banks facing higher competition tend to expand more aggressively into non- traditional activities and therefore they report higher fee income shares. We also study the relationship between banking fees and banks' performance. The results are mixed depending on applied profitability measure, but in general, banks with...