Submitted by Paula Quirino (paula.quirino@ufpe.br) on 2015-03-10T19:01:18Z No. of bitstreams: 1 DISSERTACAO VERSAO FINAL.pdf: 1786499 bytes, checksum: 5a29a7f82f0466077ab1a1f451c1b339 (MD5) / Made available in DSpace on 2015-03-10T19:01:18Z (GMT). No. of bitstreams: 1 DISSERTACAO VERSAO FINAL.pdf: 1786499 bytes, checksum: 5a29a7f82f0466077ab1a1f451c1b339 (MD5) Previous issue date: 2013 / FACEPE / O presente estudo buscou apreender parte da realidade social retratada na Decisão Fundamentada da Agência Antidoping dos Estados Unidos (USADA), na qual foram descritas todas as violações as normas do antidoping cometidas pelo respeitado e reverenciado ciclista Lance Armstrong e sua equipe a U.S. Postal. Para tal, baseou-se na análise dos testemunhos dos ex-ciclistas da referida equipe, o Código da Agência Mundial Antidoping (AMA-WADA), o histórico dos testes antidoping realizados pelo atleta, a Investigação Independente promovida pela União Ciclística Internacional sobre o teste positivo para EPO nas amostras coletadas do desportista durante o Tour de France de 1999 e o relatório técnico do teste positivo para EPO nas amostras do mesmo ano, todos estes documentos anexados a referida Decisão. Adotando uma perspectiva teórica e metodológica oriunda da sociologia simétrica de Bruno Latour, podemos perceber que a confirmação do doping do desportista só foi possível graças ao trabalho de um conjunto de híbridos tecnológicos, os quais revelaram a presença de inúmeros actantes ilícitos performando e delimitando as práticas do atleta. Deste modo, imprimiu-se um novo olhar sobre a carreira do ciclista, tendo em vista a interação deste com os seus aliados EPO (eritropoietina), Cortisona, Testosterona, Doping Sanguíneo, Actoveig, entre outros híbridos que serão apresentados no decorrer da análise. Recontar a história do ciclista sob a ótica de seus alistamentos nos fez perceber que o ciclismo, por ele praticado, não existe sem a concorrência dos distintos não-humanos que ajudam a estabilizar seu conceito, como prática desportiva. Em si tratando das provas científicas que embasaram a Decisão Fundamentada da USADA, observou-se os momentos de produção e significação da realidade nos quais o idioma performativo do doping remodelou o conceito de ciclismo profissional, praticado por Armstrong e muitos outros de sua geração.

Thesis (M.S.)--University of Wyoming, 2008. / Title from PDF title page (viewed on December 23, 2009). Includes bibliographical references (p. 119-127).

Infants in the NICU undergo many painful procedures and literature supports KMC as an effective intervention to diminish pain however, it is not used routinely in NICUs. The purpose of this qualitative study is to provide NICU nurses an opportunity to describe their experience of utilizing KMC for pain, and to interpret this experience with the goal of generating patterns that explain this understanding. The findings can be used to formulate interventions that foster the use of KMC. Using Interpretive Description as a philosophy of inquiry and research methodology, 8 NICU nurses were interviewed and participated in a focus group. Four patterns emerged: (1)”Seeing is Believing”; (2) Human Heartedness: “It’s the Least I can do”; (3) Playing it Safe; and (3) Creating the Possibility within Constraints.

The Nanoinjector Lance Array has been developed to inject foreign material into thousands of cells at once using electrophoresis to attract and repel particles to and from the electrically-charged lances. A mathematical computer model simulating the motion of attracted or repelled proteins informs the design of the nanoinjection lance array system. The model is validated by accurately predicting protein velocity in electrophoresis experiments. A complete analysis of parameters is conducted via simulations and specific research questions regarding the counter electrode of the nanoinjector lance array system are explored using the model. A novel technique for fabricating lance arrays from collapsed carbon nanotube forests is explored and detailed. Experiments are conducted using the Nanoinjector Lance Array, attempting to inject three different kinds of protein molecules into a culture of HeLa cells. The experimental results are encouraging and suggest possibilities for future success. Other recommendations are made for future research regarding the model, carbon nanotube fabrication, and experimental testing.

A novel method for charged macromolecule delivery, called nanoinjection, has been developed at Brigham Young University. Nanoinjection combines micro-fabrication technology, mechanism design, and nano-scale electrical phenomenon to transport exogenous DNA across cell membranes on a nano-featured lance. DNA is electrically accumulated on the lance, precision movements of microelectromechanical systems (MEMS) physically insert the lance into cell, and DNA is electrically released from the lance into the cell. Penetration into the cell is achieved through a two-phase, self-reconfiguring metamorphic mechanism. The surface-micromachined, metamorphic nanoinjector mechanism elevates the lance above the fabrication substrate, then translates in-plane at a constant height as the lance penetrates the cell membranes. In-vitro studies indicate no statistical difference in viability between nanoinjected and untreated mouse zygotes. Pronuclear nanoinjection experiments on mouse zygotes, using microinjection as a control, demonstrate integration and expression of a nanoinjected transgene, and higher rates of zygote survival and pup births than the microinjection control. A new compliant mechanism analysis method, the minimization of potential energy method (MinPE method) is presented to model the equilibrium position of compliant mechanisms with more degrees of freedom (DOF) than inputs, such as a fully-compliant nanoinjector. The MinPE method position and force predictions agree with the method of virtual work and non-linear finite element analyses of under-actuated and underconstrained compliant mechanisms. Additionally, a performance-based comparison is made between quadratic shell finite elements elements and 3-D quadratic solid elements for modeling geometrically non-linear spacial deflection of thin-film compliant mechanisms. The comparison's results suggest the more computationally efficient quadratic shell elements can be used to model spatially deforming thin-film compliant mechanisms. Finally, this dissertation presents preliminary results for a proposed method of DNA transfer called cytoplasm-to-pronucleus nanoinjection. By placing a DNA coated lance into the cytoplasm of a mouse zygote and applying a voltage pulse of sufficient magnitude and duration, pores may open in the pronuclear membranes and DNA may be electrophoretically repelled from the lance. If effective, this process could result in transgenes without having to visualize and physically penetrate into the pronucleus. While embryo survival has been demonstrated under a variety of injection conditions, further study is needed to increase the process' consistency, and to determine if cytoplasm-to-pronucleus nanoinjection can generate transgenic animals.

Arrays of silicon lances were made using photolithographic and STS DRIE Bosch techniques. Arrays consist of a 10 mm square grid pattern of lances measuring 100 m tall and having a 3 mm diameter, each lance being spaced 30 mm apart. The tips of lances are pointed, allowing easier penetration through plant cell walls. A nanoinjector device was also made to accept the silicon lance arrays and perform nanoinjections. A nanoinjection consisted of 2 silicon lance arrays, with lances oriented towards each other, being moved into and out of a plant cotyledon placed between them. Prior to the nanoinjection, polar molecules in solution can be attracted to the lances through a process utilizing the nanoinjector device’s ability to control the electrical current between the 2 lance arrays. During the nanoinjection the displacement between the lances, the force exerted on the plant cotyledon and the electrical current between the lance arrays are controlled. Once the lances are inserted into the cells, the electrical current between the lance arrays is reversed, repelling the molecular load from the lance array. Propidium iodide (PI) and Cotton Leaf Crumple Virus (CLCrV) were used as molecular loads in nanoinjections. The nanoinjector also records and outputs data from the nanoinjection for analysis. Nanoinjections were performed on Arabidopsis and Cotton cotyledons. Changes in the force applied during a nanoinjection and varying the number of repeated nanoinjections on the same cotyledon were observed. Too much force or too many repeated injections causes physical damage to the cotyledon. An optimal force and number of repeated injections can be performed without causing physical damage to the cotyledon. Successful transfection of PI and CLCrV was not observed in a relatively small number of performed nanoinjection procedures on either Arabidopsis or Cotton cotyledons. Possible interacting variables and recommendations for further work are discussed.

Fundamentally altering cellular function at a genetic level is a major area of interest in the biologic sciences and the medical community. By engineering transfectable constructs that can be inserted to dysfunctional cellular systems, scientists can mitigate aberrant genetic behavior to produce proper molecular function. While viral vectors have been a mainstay in the past, there are many limitations, particularly related to safety, that have changed the focus of genome editing to incorporate alternative methods for gene delivery. Lance Array Nanoinjection (LAN), a second-generation microfabricated transfection biotechnology, is one of these alternative technologies. LAN works by utilizing both simultaneous electrostatic interaction with molecular loads and physical lancing of hundreds of thousands of target cell membranes. The purpose of this work is to demonstrate LAN in the context of in vitro transfection of immortalized culture cells and primary cells. As part of that exploration, three distinct areas of investigation are considered, which include: characterizing environmental factors that impact LAN transfection, demonstrating LAN genetic modification of immortalized HeLa 229 culture cells using an indicator marker, and lastly, investigating the effects of LAN on human primary, neonatal fibroblasts.

No description available.

The sediment budget and paleogeography was reconstructed for the Maastrichtian fluvial to coastal plain Lance Formation (>200m thick) that developed coevally with the shoreline/shelf Fox Hills Sandstone (>200m thick) and deep-water Lewis Shale (>750m thick) in a complete source-to-sink system in the Washakie and Great Divide Basins of south central Wyoming. The system initiated during the final Western Interior Seaway (WIS) transgression and the onset of the Laramide Orogeny rapid subsidence (>2km in 1.9 My) that largely outpaced sediment flux into the basin so the system became and remained a deep-water (>500m water depth) basin beyond the Lance-Fox Hills shelf prism. The active tectonic setting and rapid subsidence caused the Lance fluvial and coastal plain deposits to aggrade and accumulate behind the generally rising shoreline trajectory of the Fox Hills Sandstone. The depositional succession is subdivided into 15 clinothem units and the Lance Formation is best exposed in outcrops in clinoforms 10, 11, and 12. Subsurface analysis correlates key stratigraphic surfaces across the basin to define the sediment budget and clinoform architecture. Field analysis along clinoform 12 on the east side of the basin details facies and paleohydraulic dimensions. Sediment partitioning shows the regressive and transgressive systems tracts (RST and TST) form complementary wedges such that the RST thickens basinward and the TST thins basinward, reflecting the preferential storage of sediment. Channels measured in the field and subsurface datasets are similar in thickness (2m-16m) and suggest braided channel morphology with channel belts from 6.2-8.4km. N/NE paleocurrent trends departing from the subsurface dataset and previous studies of the system provide evidence of possible tidal influences in a developed shoreline embayment or an east to west supply from the basement-cored Rawlins and Sierra Madre Uplifts in the east. The fluvial Lance Formation paleogeography associated with the RST and TST is primarily driven both by modest, Late Cretaceous relative sea level changes and sediment supply linked to the tectonic setting and climate. / text

Thesis (M.A.)--University of Nevada, Reno, 2007. / "May 2007." Includes bibliographical references (leaves 90-102). Online version available on the World Wide Web.