A commercial solid matric priming method and an osmotic priming method were used to measure seed priming responses of birdsfoot trefoil, kura clover, and sericea lespedeza. Differences were not observed using standard germination tests, but both priming methods show potential for increased germination rate (P>0.05). Conflicting results for matric and osmotic priming were found in terms of seed storage potential after priming, with matric primed seeds showing higher (P<0.05) germination after accelerated aging and osmotic primed seeds showing significant lower germination(P<0.01). Birdsfoot trefoil benefited from priming, but responses varied by priming treatment, while kura clover showed less response to both priming treatments. In a field study comparing matric primed vs. unprimed seedling emergence, matric priming effects were small and these data suggest that solid matrix priming may be unlikely to improve the field establishment of either species. Aqueous smoke solutions were also tested for effect on seed germination. Differences in final germination percent due to solution type (after exposure to liquid smoke solutions for 10- or 45-min) were not observed. Highest concentration of the 10-min solution treatment reduced (P<0.05) birdsfoot trefoil germination. Greater germination was observed only for 'Perfect Fit' kura clover treated with low or intermediate concentrations of either solution. High concentrations of 10-min smoke water increased time to 50% germination (T50) for all seeds, but some reduction in T50 occurred for kura clovers treated with low (5%) solution concentrations. The 45-min treatments had little effect on germination rates. Applying aqueous smoke solution to seeds at germination did not improve germination responses of these forage legume species. / Master of Science

Doctor of Philosophy / Department of Agronomy / Walter H. Fick / Sericea lespedeza [Lespedeza cuneata (Dumont) G. Don] is a rangeland weed of the tallgrass prairie of Kansas. Experiments were carried out during the 2001-2003 growing seasons (June-November) to examine the relationship between sericea lespedeza and other rangeland species, wildlife use and dispersal of sericea lespedeza propagules, and the relationship between sericea lespedeza stubble height and utilization by livestock. Sericea lespedeza cover was positively correlated with violet lespedeza (r=0.25) [Lespedeza violacea (L.) Pers.], heath aster (Aster ericoides L.) (r=0.23) and total forb cover (r=0.56). Sericea lespedeza composition was negatively correlated with big bluestem (Andropogon gerardii Vitman.) (r=-0.27) and sideoats grama [Bouteloua curtipendula (Michx. Torr.)] (r=-0.27) composition and positively correlated with western ragweed (Ambrosia psilostachya D.C.) (r=0.21) and violet lespedeza (r=0.36) composition. Sericea lespedeza density was inversely related to forb species richness on all sites. On half the sites, there was also an inverse relationship between grass species richness and sericea lespedeza density. Grazed sites had less difference in grass species richness between high and low levels of sericea lespedeza densities. There was no evidence of an ecological threshold to the detriment of species richness with increasing sericea lespedeza density. Cattle digestive processes did not affect sericea lespedeza germination. Quail digestive processes enhanced germination of the few seeds that were excreted. Quail diet selection was investigated with a field study. Five out of 49 crops collected contained seed classified as sericea lespedeza. None of these seeds germinated in the greenhouse. Both cattle and quail could potentially disperse sericea lespedeza seed, but voluntary consumption appears to be low for both species, at least during November when other food is available. An height-weight table estimating percent utilization for various grazed and ungrazed heights of sericea lespedeza was constructed. Coefficient of determination values were greater than 0.85 between plant height and weight, indicating that the heightweight method was appropriate for estimating sericea lespedeza utilization. Using additional regression analysis, a chart for estimating forage from percent of plants grazed was constructed.

Master of Science / Department of Agronomy / Walter H. Fick / Patch-burn grazing is a relatively new concept in terms of rangeland management. While numerous benefits have been associated with this system, in the tallgrass prairie of Kansas, cattle production and sustainability of rangeland are critical. In 2006, 253 ha at the KSU Bressner Range Research Unit in Woodson County, Kansas were subjected to spring patch-burn grazing (using one-third portions) and traditional full-burn grazing. Each treatment within the split-block design was replicated four times for 3 years. The objectives were to evaluate whether livestock performance would be compromised under this grazing system, to monitor the health of the rangeland, and to observe the usefulness of this tool as a potential control of the invasive plant sericea lespedeza [Lespedeza cuneata (Dumont) G. Don]. In regards to cattle performance, burn treatments had no significant difference in average daily gain (p≥0.10) in any of the 3 years. On average, cattle utilized 61% of the current year’s forage production in patch-burned portions, which was higher (p≤0.10) than that of unburned (30%) and full-burn (41%) portions. Results of the botanical composition show forb and woody plant composition did not differ between treatments, however differences (p≤0.10) were present in grass composition. Total annual grasses increased 19.1 percentage units under patch-burn and 2.1 units under full-burn, while total perennial grasses decreased 18.4 and 1.1 units, respectively. When evaluated by treatment area (one-third portion), results indicated that the 3-year cycle did allow enough time for recovery. At 2 years after treatment (2-YAT), no significant difference in composition(p≥0.10) was present between initial patch-burn portions and the full-burn pastures. Finally, in only 1 year of the study did cattle statistically consume a greater percentage of sericea lespedeza plants (p≤0.10) in the patch-burned portions (92%) than in full-burned pastures (35%). Biomass utilization did not differ (p≥0.10) between burn treatments. Surprisingly, there was a trend for the number of plants in the sampled areas of the patch-burn portions to decrease throughout the cycle. However, at the conclusion of the 3-year cycle, sericea densities did not differ(p≥0.10) between treatments. Patch-burn grazing shows promise as a potential management tool for Kansas land managers.

Master of Science / Department of Agronomy / Walt Fick / Prescribed burning has been a common conservation practice on native prairie dating back to the days of pioneer settlement. Advantages include increased forage quality, reduction of undesirable plants, improved wildlife habitat, removal of accumulated dead plant litter and relatively low costs. While spring is the commonly accepted time to burn, little research has been conducted on late-summer and fall burning for specific objectives that include targeting undesirable plant species and measuring potential effects on soil physical properties. The first part of this study was to evaluate the effect that prescribed burning has on population dynamics of sericea lespedeza (Lespedeza cuneata [Dumont] G. Don), rough-leaf dogwood (Cornus drummondii Meyer), and additional woody species. Stem counts and cover estimates were taken from 20, 0.25-m[superscript]2 frames prior to and post-burn. Change in botanical composition, plant density, frequency, and Daubenmire canopy cover estimates were calculated. Sericea lespedeza plant frequency across all clay upland burns decreased 2.27% and increased 4.76% across all loamy/limy upland burns the first growing season post-burn. Dogwood densities increased 3.12 stems m[superscript]-[superscript]2 on spring burns compared to a decrease of 0.30 stems m[superscript]-[superscript]2 on unburned plots the first growing season post-burn. Changes in frequency of other woody species the first growing season post-burn showed significant interactions between burn treatment and ecological site, and between ecological site and year. A significant interaction between burn treatment and ecological site was found on total woody species plant composition changes two growing seasons post-burn for the first year of burn treatments. The secondary part of this study was to evaluate the effect of prescribed burning on soil bulk density and wet-aggregate stability. Soil samples were collected along the same line-transects used for vegetation sampling. Significant differences among mean weight diameters (MWD), percent water-stable aggregates (WSA), and WSA size fractions occurred between burned and unburned soils following burning in the fall of 2011. Monitoring plant and soil response to prescribed burning in different seasons may lead to adjustments being made in management of rangelands where sericea lespedeza, dogwood, and additional woody species occur.

Feeding of lespedeza to sheep and goats, besides supplying protein, could be a possible alternative remedial control measure against gastro-intestinal parasites in sheep and goats. However, the feeding behaviour of individual animals and their feed intake are likely to influence t its effect on the parasites. This study was conducted to determine the different feeding behaviour of sheep and goats on Sericea lespedeza and its effect on faecal egg count. The first part dealt with behaviour of animals feeding on lespedeza or leucaena within the rangeland while for parasitic response they were fed on lespedeza or veld hay. During feeding behavior, three sheep and three goats averaged 2- 3 years of age were observed for 30 days during the months of March and April 2006. Activities such as browsing, grazing and idling were recorded after every two minutes for every animal while following animals at a distance of five meters. Feed intake was determined by recording the number of bites made by each animal for a period of ten minutes and by estimating the weight of forage eaten. More (P<0.001) browsing time was observed on goats at both lespedeza and leucaena plot than on sheep, with goats consistently spending longer time browsing than sheep on both lespedeza and leucaena than sheep between 7.00 and 14.00h. More of the browsing time of goats was spent on leucaena than on lespedeza. The longest time of grazing by animals was on the lespedeza plot than on leucaena plot, with sheep grazing for a longer (P<0.001) time than goats. The biggest difference in grazing time for sheep at the lespedeza and leucaena occurred during morning hours. Animal type and interaction of feed animal type significantly affected idling time with goats Idling for a longer (P<0.001) time than sheep at both the lespedeza and leucaena plots. Feeding rate (bite/min) for both sheep and goats were similar on both lespedeza and leucaena plots. Intake rates were higher (P<0.01) for both sheep and goats on leucaena than on lespedeza, with sheep having higher (P<0.01) intake rates than goats on both plots. Animals had the fastest bite rate and intake rate during midday (12.00h) and lowest rates at 14.00h. The highest intake rate occurred on leucaena in the morning and afternoon, but lower than lespedeza during midday. For parasites response twelve sheep and twelve goats aged between 3 and 4 years were used in a study that lasted 60 days. Animals were naturally infested by gastro-intestinal parasites. Egg count was done according to McMaster egg count technique (Ministry of Agriculture, Fisheries and Food, 1977) by magnifying parasitic eggs from faecal samples dissolved in saturated sodium chloride. During feacal egg count, the effect of animal on feed intake was highly significant (P<0.001) with sheep consuming more of each kind of feed (lespedeza or hay) than goats. Live weight of animals decreased during the early period of feeding, beyond which it remained stable. Egg count varied significantly (P = 0.015) among periods of sampling, a general trend indicating a decrease from day 0 to day 46 beyond which egg count tended to increase. When regression analysis was done to determine the effect of the previous week’s intake, it was observed that increased intake during the previous week was associated with depression in egg count, the effect attaining significance (P<0.05) for both sheep and goats on hay and for sheep on lespedeza. The results of this study are rather inconclusive about the effect of supplementing small ruminants with Sericea lespedeza hay on gastro-intestinal parasites, owing to variation associated with intake of lespedeza and hay, thus warranting further investigation. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

Crabgrass is a warm-season annual species that has the potential to provide high-quality summer forage for ruminants in the transition zone between subtropical and temperate regions of the United States. Growing annual lespedeza in association with crabgrass may be beneficial due to nitrogen transfer from the legume to the grass. The objectives of the research reported in this thesis were to (1) determine the effects of pH on establishment and growth of crabgrass; and (2) evaluate the effects of lespedeza seeding rate and N fertilization treatment on the yield, botanical composition, and nutritive value of crabgrass-lespedeza mixtures. A greenhouse study was conducted using three soil pH levels of 4.8, 5.5, and 6.3. Crabgrass germination and root and shoot yields were not affected by soil pH values. A field study was conducted to evaluate the influence of six lespedeza seeding rates (0-28 kg ha-1) and two N fertilization treatments (140 kg total N ha-1 or zero N) on crabgrass-annual lespedeza mixtures. In most cases, increasing lespedeza seeding rate increased lespedeza in the sward. However, lespedeza rate had limited effect on yield and nutritive value of the mixture. Nitrogen fertilization increased crabgrass in the sward and total yield by as much as 46%. Responses of nutritive value parameters to N fertilization were variable and appeared linked to weather factors. In vitro true digestibilities ranged from 750 to 875 g kg-1, and were largely unaffected by N fertilization and seeding rate The results of these studies indicate that crabgrass could provide moderate amounts of highly digestible forage while growing on acidic soils commonly found in the southeastern United States. Annual lespedeza may be grown in association with crabgrass, but limited improvement in yield and nutritive value were found for this practice. / Master of Science

Cuscuta pentagona is an obligate parasitic plant that hinders production of crops throughout the world. Parasitic plants have unique morphological and physiological features, the most prominent being the haustorium, a specialized organ that functions to connect them with their host's vascular system. The Cuscuta haustorium is remarkable in that it enables mRNA movement to occur between hosts and parasite, but little is known about the mechanisms regulating cross-species mRNA transfer or its biological significance to the parasite. These questions were addressed with genomics approaches that used high throughput sequencing to assess the presence of host mRNAs in the parasite as well as parasite mRNAs in the host. For the main experiment Cuscuta was grown on stems of Arabidopsis thaliana and tomato (Solanum lycopersicon) hosts because the completely sequenced genomes of these plants facilitates identification of host and parasite transcripts in mixed mRNA samples. Tissues sequenced included the Cuscuta stem alone, the region of Cuscuta-host attachment, and the host stem adjacent to the attachment site. The sequences generated from each tissue were mapped to host reference genes to distinguish host sequences, and the remaining sequences were used in a de novo assembly of a Cuscuta transcriptome. This analysis revealed that thousands of different Arabidopsis transcripts, representing nearly half of the expressed transcriptome of Arabidopsis, were represented in the attached Cuscuta. RNA movement was also found to be bidirectional, with a substantial proportion of expressed Cuscuta transcripts found in host tissue. The mechanism underlying the exchange remains unknown, as well as the function of mobile RNAs in either the parasite or host. An approach was developed to assay potential translation of host mRNAs by detecting them in the Cuscuta translatome as revealed by sequencing polysomal RNA and ribosome-protected RNA. This work highlights RNA trafficking as a potentially important new form of interaction between hosts and Cuscuta. / Ph. D.