This thesis, which is a continuation of the previous Bachelor thesis, is dedicated to the study of polyelectrolyte complexes between hyaluronan (HA) and bovine serum albumin (BSA). Interactions between HA and BSA and a formation of complexes were studied by SEC-MALLS method, where a molar mass, a root mean square (rms) radius, a hydronamic radius and an intrinsic viscosity of particles of the system were defined. Furthermore the interactions were studied by calorimetric measurement ITC, where thermodynamic character of complex formation was determined. Additionally, dynamic and electrophoretic light scattering methods (DLS and ELS) were applied, where hydrodynamic radius and a value of the zeta potential were defined. Also the effect of higher temperature on the character and complex formation was examined by SEC-MALLS and ITC measurements. The interactions were confirmed at any of the used media, but with different efficiency. It was also proved that complexes become smaller in their radii and viscosity with growing BSA concentration. Furthermore it was proved, that the high ionic strength hinders surface charges of HA and BSA molecules and minimizes their mutual interactions. At the higher ionic strength the radii of the complexes, their intrinsic viscosity and zeta potential are increased. The higher temperature has only minimal effect on the formation of the complexes.

It is well established that national retail chains impact small, single location retail businesses in terms of revenue generation, retail structure, retail type diversity, and location. This study examines the retail structure and diversity of five retail districts in the City of Denton, Texas. The analysis focuses on one central business district (CBD), one traditional retail strip center (University Drive, also known as US HWY 380), one special retail district (Fry Street District), one traditional enclosed shopping mall and associated development (Golden Triangle Mall), and one power retail center (Denton Crossing). The empirical foundation for the investigation is a historical business database covering years 1997 to 2010, obtained from Info Group's Reference USA. This Reference USA database includes location, industry, and status (single versus chain location) information for each business. Retail diversity and evenness were measured for each of the five retail districts using the Simpson's Diversity Index and the Simpsons Measure of Evenness, leading to specification of the differences that exist in retail structure and diversity among the districts. Golden Triangle Mall and Denton Crossing were primarily chain location in composition while Fry Street District, the CBD, and University Drive were primarily single location in composition. Across all years, the single versus chain status of the local business communities did not substantially change within any of the districts. The Fry Street District exhibited the most change in diversity as well as the lowest overall diversity among the retail districts, followed by University Drive and Golden Triangle Mall. The CBD did not experience any major change in retail type diversity. However, all retail districts experienced major changes in retail evenness. Overall for the city, single location retail businesses accounted for the majority of all the retail businesses, however, chain locations employed more people. In total, these findings indicate that the development of retail districts composed primarily of chain location retailer's affects retail district diversity and evenness but not retail structure.

Le virus de la stomatite vésiculaire (VSV) sert de modèle pour l'étude de la multiplication des virus (Mononegavirales) alors que la rage(RV) reste un sérieux problème de santé publique. Le génome de VSV et RV code notamment la nucléoprotéine (N) et la phosphoprotéine (P). N s'associe étroitement à l'ARN viral. Ce complexe N-ARN sert de matrice pour la réplication et la transcription virale. P est le cofacteur de la polymérase virale (L) et chaperonne N. En interagissant avec N-ARN (domaine C-terminal) et avec L (domaine N-terminal), P assure le lien physique entre l'ARN viral et L. La stœchiométrie de P, sa structure et son rôle exact pendant la transcription et la réplication restent incertains. Mon travail a consisté à une caractérisation biophysique et structurale de P et des complexes N-ARN-P pour mieux comprendre la dynamique du complexe de réplication de ces virus.<br />L'analyse biophysique montre que P RV & VSV existent sous forme de dimère allongé en solution. L'analyse bioinformatique a révélé une organisation modulaire, confirmé par des études biochimiques et biophysiques de mutants de P RV. La structure du domaine C-terminal de P VSV a été résolue par RMN et montre une homologie celle du C-ter de P RV. La caractérisation de l'interaction entre P et les anneaux N-ARN a révélé l'existence de deux types de complexes N-ARN-P (contenant un et 2 dimères de P par anneau). L'étude par ME des complexes nucléocapsides-P a permis de mettre en évidence un changement de conformation important.<br />Pour devenir accessible à L, l'ARN viral doit se dissocier localement de N. L'interaction N-ARN-P représente potentiellement une nouvelle cible pour le développement d'antiviraux.

The private security industry is tasked with protecting lives and property against an evolving array of personal and property threats. Rendering such services comes with many market related challenges for private security providers. These services are rendered to various types of clients like government departments, hospitals, universities and shopping malls amongst others. These clients have unique and different needs and expectations, therefore shopping malls as clients of the private security industry were selected for the purpose of this research. The qualitative research approach was used for this study wherein a questionnaire was used to obtain information from private security and shopping mall personnel working in shopping malls in Gauteng. The findings of this study reveals that there are indeed client retention challenges that are facing private security companies providing services to shopping malls. Based on the findings, recommendations for the private security providers and further research in shopping mall security management are made. / Security Risk Management / M.Tech. (Security Management)

The specified area is located in the city district Brno - Židenice, near the railway station. The theme of the thesis is the architectural study of selected part of the multifunctional intense urban structure, which was solved in the pre-diploma project. Draft uses the building site intensively, provides high plot ratio and density of population, a wide range of functions in a relatively small area is related to the elimination of transportation. By using topography the proposal creates a permeable areas, buildings with relations to the surroundings and with quality public spaces and attractive housing. Pick-up railway cancels barrier and makes a space with landscaping. Readability is achieved by linking the territory from east to west by straight streets and from north to south by passages. The development creates three centers, the commercial center of the north, center of culture in the heart of the area and the center of relaxation and sport in the south. Basic grid of development has a checkerboard plan, which reflects the grid of surrounding structure. The new development varies in its scale, from large scale buildings in the parterre decays into smaller scale. Function division is horrizontal. There are underground parking, a parterre with shops and services, a floor with offices and work study open to the courtyard and housing on the upper floors. Thus formed structure provides enough light in the courtyards and flats. The segmentation is supported by materials, bricks in the parterre contrast with the monotone plaster on the upper floors.