Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.

No description available.

No description available.

No description available.

No description available.

No description available.

No description available.

Meat Juice Medium (MJM), an aqueous extract of meat, was inoculated with Pseudomonas aeruginosa and incubated for 7 d at 4$ sp circ$C under shaking conditions (100 rev.min$ sp{-1}$). Two predominant compounds produced during spoilage of MJM were detected using HPLC. These compounds with retention times (RT) of 21.48 and 32.04 min were tentatively identified as acetic and butyric acids, respectively. These compounds were also produced when MJM was replaced with Brain Heart Infusion Broth medium. In later experiments, the effect of glucose supplementation on the rate of MJM spoilage was examined. Glucose 0.5% (wt/vol) was added to the MJM, inoculated with P. aeruginosa and incubated at 30$ sp circ$C under shaking conditions (100 rev.min$ sp{-1}$). HPLC of samples after 1d of incubation indicated the presence of 8 predominant compounds including acetic and butyric acids. Their concentrations were, in general, higher in control samples of MJM without added glucose. Using HPLC, TLC, Pyrolysis/GC/MS, FTIR and GC-MS methodologies, the compounds with RT of 8.91, 9.67, 11.96, 13.33, 17.74, 21.48, 26.07 and 32.04 min were tentatively identified as cadaverine, 2-keto gluconic acid, fructose, lactic acid, acetic acid, methanol and butyric acid. In contrast to the results of previous researchers, cadaverine was produced in large amounts while no putrescine was produced by P. aeruginosa. During the spoilage period, the levels of glucose, fructose and total carbohydrate were monitored. Addition of glucose to MJM delayed slime production by 4 days and increase to maximum pH of 8.3 by 7 days. Results suggest that addition of glucose to MJM delays spoilage by P. aeruginosa.

No description available.

This study investigated the hypothesis that cattle fed to produce a lean carcass during a winter restriction period will subsequently grow faster and remain leaner than fatter steers during the summer, exploiting cheap grazed grass. Three studies were carried out; in the first study steers were restricted at three levels of growth (300, 600 and 900 g/day) during winter and turned out to graze grass during summer. At each level of growth during winter steers were fed on one of two diets aimed at producing steers of either a lean or fat carcass composition. At the end of the winter restriction period differences in lean composition were observed at the 300 and 600 g/day growth rates. Steers were slaughtered when they attained the target slaughter weight at the end of summer; but there was no difference between treatments in meat quality characteristics or fat composition. Liveweight gain during the summer period was inversely correlated to winter liveweight gain. Two further studies were carried out to assess the effect of altering carcass composition during winter, fed to a predicted growth rate of 600g/day to investigate the underlying physiological and endocrinological mechanism regulating the growth characteristics. In the first study at the end of winter steers that were fatter had higher glucose and insulin concentrations. No differences between diet treatments were detected in muscle protein synthesis or breakdown. At the end of the winter in the second experiment there were no differences in carcass composition, metabolite or hormone profiles between treatments. For both studies, at the end of summer, steers on different dietary treatments had similar carcass compositions and metabolic parameters. It was concluded that by altering the diet of steers during a winter restriction period the composition of carcass gain can be manipulated. Since steers altered the deposition of carcass protein and fat during the subsequent summer period at grass resulting in similar body compositions at slaughter it was deduced the that composition of steers at the end of winter remains unimportant. Reductions in beef production costs may be attained by restricting the growth rates during winter when commercial feeds are expensive.