Bacterioplankton provide important ecosystem functions by carrying out biogeochemical cycling of organic matter. Playing an important role in the microbial loop they help remineralize carbon and nutrients. Bacteria also interact with phytoplankton during phytoplankton blooms. However, fundamental understanding on the underlying molecular mechanisms involved in the degradation of phytoplankton-derived organic matter is still in its infancy. Therefore, we analysed data from a mesocosm experiment following a natural phytoplankton-bloom from an upwelling system in the North- East Atlantic Ocean. The purpose was to contribute a mechanistic understanding based on functional gene expression analysis of natural microbial assemblages. Our results show the difference in functional gene expression within a bacterial metacommunity and how this functional response drastically switches between bloom build up and senescence. Transcripts showed a broad change in gene expression involving major SEED categories, with the bloom senescence phase exhibiting a higher relative abundance in major categories such as Carbohydrates, Protein Metabolism and Amino Acids and Derivatives. Within these categories genes connected to carbon utilization and transport systems (Ton and Tol) as well as chemotaxis showed a higher abundance during bloom senescence. The change in functionality based on transcripts showed a different bacterial community composition appearing over a very short time. We thus conclude that the bacterial functional gene expression response between build-up and degradation bloom phases is remarkably different and associated with a change in the identity of bacteria with active expression. Our findings highlight the importance of bacterial substrate specialists with different functional roles during different time points of phytoplankton blooms.

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Cyclodipeptides (CDPs) are the smallest, most stable cyclic peptides that are synthesized as secondary metabolites by bacteria. The aim of this study was to investigate the effect of the Pseudomonas argentinensis (SA190) and four (CDPs), named as cis-cyclo-(Pro-Phe) (Cyclo2), cis-cyclo-(Pro-Leu) (Cyclo3), cis-cyclo-(Pro-Tyr) (Cyclo4) and cis-cyclo-(Pro-Val) (Cyclo5), with three concentrations (1µM, 100nM, and 10nM), on the growth of Arabidopsis thaliana under normal plant growth conditions [1/2MS media], salt conditions [125 mM NaCl] and drought conditions [25% PEG]. Moreover, we determined the most effective CDPs with optimal concentration. It was found that cis-cyclo-(Pro-Tyr) (Cyclo4) at a concentration of 100nM had an effect on the plant growth and can mimic the effect of SA190 under normal [1/2MS media] conditions. Also, cis-cyclo-(Pro-Tyr) (Cyclo4) at a concentration of 1µM can mimic the effect of SA190 under salt conditions [125mM NaCl]. Finally, cis-cyclo-(Pro-Val) (Cyclo5) at a concentration of 1µM can mimic the effect of SA190 under drought conditions [25% PEG].

This project explores co-being and interdependencies between human and more-than-human, the microbes, through the medium of fermentation and the (hidden) communities this practice embodies. Therewith not only resisting commodification and alienation from our food but facing our very own identity, and the human-made construct of human exceptionalism and detachment of nature and culture. The cells in ’our’ human body are outnumbered by the cells of other microorganisms. They even actively influence many of the bodily functions associated with the concept of ’self‘ (our brain, immune system and genome).1 Considering this, what does it even mean to be human? What does it mean to be me, If not cherishing and embracing the more-than-human, more-than-one-culture collective? The Korean word 손맛 ’son-mat’/ ’hand-taste’ refers to the inherited quality, love and care that went into preparing the (often associated with mother‘s) dish, something uniquely connected to the cook. While the microbes in sourdough can be linked to the baker‘s hand microbes, the baker‘s microbes have also shown to beaffected by the interaction with sourdough (Herman‘s (see picture to the right) microbial culture) with the scientific findings exposing our mutual interaction. This son-mat within fermentation I see as a symbolized touching point where our human realm and the microbial invisible microcosmos meet and become tangible. To emphasize this co-being, I work with our bodily senses, using design to bridge, making the insensible sensible, tangible, and audible. Staying curious and sprawling with my design approaches of creating narratives with the more-than-human, aimed to evoke questions and reflections of us and our culture. What happens when we share culture (human and microbial)? Through our hands, eating and digesting parts of others and becoming-with. To share culture means to see that humans and ’non-humans‘ are one. To taste that our culture is shared. And to feel that nature and culture are not two but one. Can you taste it?

Microbial cell disruption using pressurized gas is a promising approach to improve the lipid extraction yield directly from the wet biomass by eliminating the energy-intensive drying process, which is an integral part of traditional methods. As the process starts with the solubilization of the gas in lipid-rich microbial cells, it is important to understand the solubility of different potential gases in both lipid (triglyceride) and lipid-rich microbial cell culture to design efficient cell disruption processes. In this study, we determined the solubility of different gases (e.g., CO2, CH4, N2, and Ar) in canola oil (triglyceride) using a pressure drop gas apparatus developed in our laboratory. The solubility of different gases in triglyceride followed the trend CO2 > CH4 > Ar > N2. Since the solubility of CO2 was found to be higher compared to other gases, the solubility of CO2 in lipid rich cell culture, cell culture media, and spent media was also determined. It was found that CO2 is more soluble in triglycerides, but less soluble in lipid-rich cell culture compared to CO2 in water. From both thermodynamic models and Monte Carlo simulations, the correlated solubility was found to be in good agreement with the experimental results. CO2 was found to be the most suitable gas for microbial cell disruption because almost 100% cell death occurred when using CO2 whereas more than 85% cells were found to be active after treatment with CH4, N2, and Ar. The optimization of microbial cell disruption was conducted using the combination of Box-Behnken design of experiment (DOE) technique and response surface methodology. The optimized cell disruption conditions were found to be 3900 kPa, 296.5 K, 360 min, and 325 rpm where almost 100% cell death was predicted from the statistical modeling. Finally, it was found that 86% of the total lipid content can be recovered from the wet biomass after treatment with pressurized CO2 under optimized conditions compared to control where up to 74% of the total lipid content can be recovered resulting in 12% increase in the lipid extraction yield using pressurized CO2.

No description available.

Various disorders closely linked to gut dysbiosis have been associated with poor dietary patterns. Dietary prebiotic fibers play an essential role in modulating the gut microbiome by enhancing the abundance of beneficial microorganisms and improving the production of short-chain fatty acids. Arabinoxylan (AX) is a major component of most dietary fibers and has been shown to exhibit potential prebiotic properties and modulate gut microbiome composition. This study aimed to investigate the in vitro impact of bioengineered wheat arabinoxylan on depression-inversely linked gut microbes and human gut microbiome diversity and metabolism. This study demonstrates the ability of bioengineered AX to stimulate the growth of depression-inversely linked gut bacterial species (Faecalibacterium prausnitzii and Lacticaseibacillus rhamnosus LGG). On the microbiome composition, the bioengineered AX induced an increased abundance of beneficial bacterial taxa (Bacteroides, Bifidobacterium, Anaerofustis, and Eubacterium) compared to the control and native AX. These effects on microbes translated into significant metabolic activity and produced primary SCFAs (acetate, butyrate, and propionate). The findings from this study suggest that bioengineered wheat arabinoxylan could be considered a promising strategy for fostering next-generation prebiotics targeting depression-inversely linked gut microbes and also supports the structure-function relationship between AX and the human gut microbiome.

Human disruption of climate, habitat, and ignition has altered the behavior of wildland fire at local to continental scales. In many regions, novel fire regimes are emerging that threaten to exceed the capacity for local management to protect human wellbeing and ecosystem function. Simultaneous changes in climate, species composition, and fire management have resulted in extreme fire behavior in many regions. For the Western United States, the emerging novel fire regime consists of more frequent, severe, and intense wildfires, with annual area burned by wildfire having doubled and high-severity wildfire area having increased 8-fold since the 1980s. The impacts of these increasing stresses in the Great Basin is especially pressing when combined with the many years of historically poor resource management. Here we complete a literature review of changing wildfire regimes globally (chapter 1) and a study of how the abiotic and biotic aspects of aquatic ecosystems stabilize after a megafire in the western United States (chapter 2).

Editorial on the Research Topic. Antimicrobial and Anticancer Peptides.

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