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Developing a Guide and Template to Aid the Preparation of Mosquito Surveillance Plans in OhioFlynn, Rebecca Anne 16 July 2018 (has links)
No description available.
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Factors influencing U.S. canine heartworm (Dirofilaria immitis) prevalenceWang, Dongmei, Bowman, Dwight, Brown, Heidi, Harrington, Laura, Kaufman, Phillip, McKay, Tanja, Nelson, Charles, Sharp, Julia, Lund, Robert January 2014 (has links)
BACKGROUND:This paper examines the individual factors that influence prevalence rates of canine heartworm in the contiguous United States. A data set provided by the Companion Animal Parasite Council, which contains county-by-county results of over nine million heartworm tests conducted during 2011 and 2012, is analyzed for predictive structure. The goal is to identify the factors that are important in predicting high canine heartworm prevalence rates.METHODS:The factors considered in this study are those envisioned to impact whether a dog is likely to have heartworm. The factors include climate conditions (annual temperature, precipitation, and relative humidity), socio-economic conditions (population density, household income), local topography (surface water and forestation coverage, elevation), and vector presence (several mosquito species). A baseline heartworm prevalence map is constructed using estimated proportions of positive tests in each county of the United States. A smoothing algorithm is employed to remove localized small-scale variation and highlight large-scale structures of the prevalence rates. Logistic regression is used to identify significant factors for predicting heartworm prevalence.RESULTS:All of the examined factors have power in predicting heartworm prevalence, including median household income, annual temperature, county elevation, and presence of the mosquitoes Aedes trivittatus, Aedes sierrensis and Culex quinquefasciatus. Interactions among factors also exist.CONCLUSIONS:The factors identified are significant in predicting heartworm prevalence. The factor list is likely incomplete due to data deficiencies. For example, coyotes and feral dogs are known reservoirs of heartworm infection. Unfortunately, no complete data of their populations were available. The regression model considered is currently being explored to forecast future values of heartworm prevalence.
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Evolutionary ecology of reproductive strategies in malaria parasitesCarter, Lucy Mary January 2014 (has links)
For vector-borne parasites such as malaria, how within- and between-host processes interact to shape transmission is poorly understood. In the host, malaria parasites replicate asexually but for transmission via mosquitoes to occur, specialized sexual stages (gametocytes) must be produced. Once inside the mosquito vector, gametocytes immediately differentiate into male and female gametes, and motile male gametes must swim through the hostile environment of the bloodmeal to find and fertilise female gametes. Despite the central role that gametocytes play in disease transmission, explanations of why parasites adjust gametocyte production in response to in-host factors remain controversial. Furthermore, surprisingly little is known about the mating behaviour of malaria parasites once inside the mosquito. Developing drugs and/or vaccines that prevent transmission by disrupting sexual stages are major goals of biomedicine, but understanding variation in gametocyte investment and male gamete behaviour is key to the success of any intervention. First, I propose that the evolutionary theory developed to explain variation in reproductive effort in multicellular organisms provides a framework to understand gametocyte investment strategies in malaria parasites. I then demonstrate that parasites appear to change their reproductive strategies in response to environmental cues and in a manner consistent with our predictions. Next, I show how digital holographic microscopy can be used to characterise the morphology and motility of male gametes. I then provide evidence for non-random movement of male gametes and that gamete interactions with red blood cells appear to hinder mating success in a bloodmeal. Finally, I discuss the variation in gametocyte differentiation and fertilisation success when exposed to a number of factors implicated in gametocyte activation. The data presented here provides important information on the basic biology of malaria parasite reproductive stages and demonstrates considerable variation in parasite traits and behaviours in response environmental changes; both in the host and in the mosquito vector.
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Characterisation of the response of Aedes mosquito cells to Semliki Forest virus infectionSiu, Ricky Wai Chi January 2012 (has links)
Arboviruses are transmitted to vertebrates by arthropod vectors such as mosquitoes or ticks. The replication of Semliki Forest virus (SFV) (Togaviridae; Alphavirus) in vertebrate cells is well established and triggers cell death. SFV infection of Aedes albopictus mosquito cells was characterised. Virus growth curves were compared in three cell lines. Infection of U4.4 cells was persistent and did not affect growth of the culture. In contrast, infection of C6/36 and C7-10 cells resulted in a static culture with no cell division and no cell death. The response of U4.4 cells was characterised in greater detail using viruses containing fluorescent or luciferase markers within the replicase or structural open reading frame of the virus genome. Activation of the STAT/IMD pathway prior to SFV infection significantly reduced virus driven luciferase expression and virus production. Activation of the Toll pathway prior to SFV infection had no effect. However, activation of Toll in addition to STAT/IMD had a cumulative effect on luciferase expression and virus production. viRNAs were characterised by Illumina Solexa sequencing. Two percent of the small RNA species found in virus infected cells were derived from virus RNA. These were predominantly 21 nt long and mapped along the entire SFV genome and genome complementary RNAs. Generation of these viRNAs was not random. Some areas produced high frequencies and others no or very few; hot and cold spots respectively. There were no correlations between viRNA frequency and base pairing or secondary structures predictions. Cold spot-derived viRNAs were more effective than hot-spot viRNAs in inhibiting virus replication. Similar results were observed in Aedes aegypti-derived cells. Attempts were made to investigate the source of these viRNAs using a virus containing an IRES element which had been reported to prevent virus replication in insect cells but which did not efficiently do so in this study. A virus containing the RNAi inhibitor p19 was characterised and shown to increase virus production. Techniques for infecting mosquitoes via a blood meal feed were established. No infection was observed with virus replicon particles carrying a fluorescent marker gene. Infection was established using virus containing p19.
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Genomic, structural and functional characterization of odorant binding proteins in olfaction of mosquitoes involved in infectious disease transmission / Caractérisation génomique, structurale et fonctionnelle des protéines liant les molécules odorantes dans le système olfactif des moustiques vecteurs de maladies infectieusesManoharan, Malini 28 September 2011 (has links)
Dans le système olfactif des moustiques, les protéines liants les molécules odorantes ou odorant binding proteins (OBPs) interviennent dans les toutes premières étapes permettant d'aboutir à la reconnaissance de leurs hôtes et font l'objet d'un intérêt croissant dans les recherches sur la transmission des maladies infectieuses par ces insectes. Le travail présenté a pour objet d'approfondir les connaissances sur ces OBPs dans trois génomes de moustiques, tous vecteurs de maladies infectieuses : Anopheles gambiae, Aedes aegypti et Culex quinquefasciatus. Une analyse à l'échelle de ces génomes a été réalisée et a permis d'identifier un nombre important de nouveaux gènes d'OBPs notamment chez les espèces de moustiques Aedes aegypti et Culex quinquefasciatus. Complétée par une étude phylogénétique du répertoire complet de ces gènes dans les trois génomes étudiés, cette analyse a permis d'établir une nouvelle classification des sous familles des OBPs. Ce résultat démontre l'extraordinaire multiplicité et diversité des gènes impliqués dans l'olfaction chez ces espèces de moustiques tout en mettant en lumière certaines propriétés des séquences des OBPs qui sont hautement conservés chez les moustiques. Grâce à la disponibilité de certaines structures d'OBPs de moustiques ou d'autres insectes apparentées, des modèles structuraux de tous les OBPs de la sous famille dites Classic dans les trois génomes, soit au total 137 structures, ont été construits. Ces structures ont servi de base pour le criblage à grande échelle par docking moléculaire d'une chimiothèque de 126 molécules odorantes connues pour leurs propriétés attractives ou répulsives vis-à-vis des moustiques. Ces résultats fournissent pour la première fois, les bases structurales et fonctionnelles pour la compréhension au niveau moléculaire de l'efficacité de certains agents répulsifs tout comme de l'attractivité de certains agents provenant des émanations humaines. Par simulation de dynamique moléculaire, les changements qui s'opèrent dans une de ces OBPs lorsque celle ci, liée à une molécule odorante, se retrouve dans des conditions de pH modifiée ont été caractérisée et un mécanisme probable par lequel ces OBPs participeraient à la reconnaissance et la libération des molécules odorantes est proposée. Cette thèse fournit des éléments de réponses importants quant à la caractérisation génomique, structurale et fonctionnelle des OBPs de moustiques et peut servir de base de départ pour des recherches expérimentales plus approfondies sur ces aspects. / The role of odorant binding proteins in the olfaction of mosquitoes, the primary mechanism of human host recognition, has been an important focus of biological research in the field of infectious disease transmission by these insects. This thesis provides an in depth knowledge of these proteins in three mosquito species Anopheles gambiae, Aedes aegypti and Culex quinquefasciatus. A large scale analysis on these genomes has been carried out towards the identification of the odorant binding proteins in the mosquito genomes. Identification of many new OBP members, in particular in the Aedes aegypti and Culex quinquefasciatus species, and an extensive phylogenetic analysis presenting a novel classification of the OBP subfamilies of these mosquito species has been proposed. This results further demonstrates the extraordinary multiplicity and diversity of the OBP gene repertoire in these three mosquito genomes and highlights the striking sequence features that are nevertheless highly conserved across all mosquito OBPs. Owing to the availability of homologous structures from mosquitoes or related species, the 3D structure modelling of all the Classic OBPs from the three genomes (representing in total 137 structures) has been performed. This was completed by large scale docking studies on these structures by screening a large set of compounds that are known to be mosquito attractants or repellents. These provide many exciting new insights into the structural and functional aspects towards understanding the efficacy of some repellents and of some attractants from human emanations. Through molecular dynamics simulation, the structural changes observed in an OBP bounded to an odorant when pH conditions are modified were characterized and the probable mechanism of ligand binding and release is presented. This work provides the first insights to many of the long awaited questions on the genomic, structural and functional characterization of mosquito OBPs and can be viewed as a reliable starting point for further experimental research focussed on these aspects.
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Metody odhadů složitosti důkazů ve výrokové logice / Methods of proving lower bounds in propositional logicPeterová, Alena January 2013 (has links)
In the present work, we study the propositional proof complexity. First, we prove an exponential lower bound on the complexity of resolution applying directly Razborov's approximation method, which was previously used only for bounds on the size of monotone circuits. Next, we use the approximation method for a new proof of an exponential lower bound on the complexity of random resolution refutations. That should have further applications in separating some theories in bounded arithmetic. In both cases, we use a problem from the graph theory called Broken Mosquito Screens. Finally, we state a conjecture that the approximation method is applicable even for stronger propositional proof systems, for example Cutting Plane Proofs. Powered by TCPDF (www.tcpdf.org)
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Struktura a využití Guerilla marketingu v praxi / Structure and application of guerilla marketing in practiseErtelt, Marek January 2009 (has links)
Dissertation "Structure and application of guerilla marketing in practise" is concerned with detailed analysis of different types of guerilla marketing. It finds recommendation for an application of each type in dependence on the type of company. It uses a lot of real examples for this purpose. This dissertation also offers modern rules for creating efficient and succesfull guerilla campain. It is also concerned with the guerilla marketing legislation.
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Insights into vector control through the modulation of An. gambiae G protein-coupled receptorsRegna, Kimberly January 2015 (has links)
Thesis advisor: Marc A.T. Muskavitch / Malaria is a life-threatening infectious disease caused by inoculation of the apicomplexan Plasmodium parasite into vertebrate hosts. Transmission of the parasite is mediated by the Anopheles mosquito, which has the capacity to efficiently transmit the parasite from host to host, as the disease vector. There are many factors that make anopheline mosquitoes competent vectors for disease transmission. The hematophagous (blood-feeding) behavior of the female mosquito is one of most fundamental factors in physical transmission of parasites, because the ingestion of blood from an infected host allows parasite entry into the mosquito and the completion of parasite sexual reproduction. In addition to this blood-feeding behavior, there are a host of biological (i.e., parasite replication) and behavioral factors (i.e., mosquito chemosensation, host preference) that contribute to the high vectorial capacity of these vector species. There are over four hundred Anopheles species worldwide, approximately forty of which are considered epidemiologically critical human malaria vectors. Anopheles gambiae, the primary vector in malaria-endemic sub-Saharan Africa, is responsible for the largest number of malaria cases in the world and is therefore one of the most important vectors to study and target with control measures. Currently, vector-targeted control strategies remain our most effective tools for reduction of malaria transmission and incidence. Although control efforts based on the deployment of insecticides have proven successful in the past and are still widely used, the threat and continuing increases of insecticide resistance motivate the discovery of novel insecticides. In this thesis, I provide evidence that G protein-coupled receptors (GPCRs) may serve as “druggable” targets for the development of new insecticides, through the modulation of developmental and sensory processes. In Chapter II, “A critical role for the Drosophila dopamine 1-like receptor Dop1R2 at the onset of metamorphosis,” I provide evidence supporting an essential role for this receptor in Drosophila melanogaster metamorphosis via transgenic RNA interference and pharmacological methods. In An. gambiae, we find that the receptor encoded by the mosquito ortholog GPRDOP2 can be inhibited in vitro using pharmacological antagonists, and that in vivo inhibition with such antagonists produces pre-adult lethality. These findings support the inference that this An. gambiae dopamine receptor may serve as a novel target for the development of vector-targeted larvicides. In Chapter III, “RNAi trigger delivery into Anopheles gambiae pupae,” I describe the development of a method for injection directly into the hemolymph of double strand RNA (dsRNA) during the pupal stage, and I demonstrate that knockdown of the translational product of the SRPN2 gene occurs efficiently, based on reductions in the levels of SRPN2 protein and formation of melanized pseudo-tumors, in SRPN2 knockdown mosquitoes. This method was developed for rapid knockdown of target genes, using a dye-labeled injection technique that allows for easy visualization of injection quality. This technique is further utilized in Chapter IV, “Uncovering the Role of an Anopheles gambiae G Protein-Coupled Receptor, GPRGR2, in the Detection of Noxious Compounds,” where the role for GPRGR2 in the detection of multiple noxious compounds is elucidated. We find that pupal stage knockdown of this receptor decreases the ability of adult Anopheles gambiae to identify multiple noxious compounds. While these findings provide a strong link between GPRGR2 and a very interesting mosquito behavior, they may also provide opportunities to develop better field-based strategies (i.e., insecticides baited traps) for vector control. The goal of this thesis is to understand the functional roles of selected mosquito GPCRs that may serve as targets for the development of new vector-targeted control strategies. Exploiting these GPCRs genetically and pharmacologically may provide insights into novel vector control targets that can be manipulated so as to decrease the vectorial capacity of An. gambiae and other malaria vectors in the field, and thereby decrease the burden of human malaria. / Thesis (PhD) — Boston College, 2015. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Biology.
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Métodos de coletas de mosquitos (Diptera: Culicidae) alternativos ao de atração humana diretaVIEIRA, Paoola Cristina Bezerra January 2007 (has links)
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Previous issue date: 2007 / Vários métodos de captura de mosquitos foram desenvolvidos,
demonstrando que houve uma evolução dos tipos de armadilhas ao longo do
tempo, porém nenhum se comparou à coleta direta por Atração Humana (AH).
Este trabalho teve por objetivo, testar métodos alternativos, desenvolvidos para
coletar mosquitos, incluindo o desenvolvimento de novas técnicas, que podem
substituir o método tradicional de captura direta em humanos. O estudo foi
desenvolvido próximo ao Parque Ambiental de Belém, Pará, Brasil. Os métodos
utilizados foram: armadilhas CDC, CDC UV, Shannon, armadilha desenvolvida
por J. A. Rocha, armadilha desenvolvida por I. S. Gorayeb e coleta por AH. As
armadilhas foram instaladas em seis pontos, por seis noites consecutivas. Foram
capturados 13.099 espécimes de Culicidae distribuídas em sete gêneros e 27
espécies, sendo Anopheles trianulatus a mais abundante. Os dados foram
tratados estatisticamente pelos métodos ANOVA, Análise multivariada –
distâncias euclidianas. A coleta por AH direta foi o método que coletou maior diversidade e abundância de mosquitos, seguidos da armadilha de Shannon para abundância e armadilha de Gorayeb para diversidade. / Some methods capture of mosquitoes already had been developed,
demonstrating that it had an evolution of the types of the trap to the long one of
the time, however none has shown to be comparable to the direct human
attraction (AH). This work had for objective, to test the methods alternative,
developed to collect mosquitoes, including the development of new techniques,
that can substitute the traditional method of direct human attraction. The study it
was developed next to “Parque Ambiental de Belém”, Pará state, Brazil. The
used methods were: traps CDC, CDC UV, Shannon, trap developed by Rocha,
trap developed by Gorayeb and collect for AH direct. The traps were installed in
six points, for six consecutive nights. Were captured 13,099 specimens of
Culicidae of seven genera and 27 species, being Anopheles trianulatus the most
abundant. The direct human attraction, what it collected greater diversity and
abundance of mosquitoes, followed of the Shannon’s trap for abundance and
Gorayeb’s trap for diversity.
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Identificação das fontes alimentares de mosquitos transmissores da malária na Amazônia brasileira pela técnica de Bloodmeal ELISACARMO, Auristela Ramos do 07 July 2006 (has links)
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Previous issue date: 2006 / O ensaio imunoenzimático para identificação de repastos sangüíneos apresenta especificidade até nível de gênero, sensibilidade para identificação de repastos sangüíneos parciais e detecção de repastos múltiplos. Foram identificadas as fontes alimentares de 82% dos anofelinos coletados em campo. Foram testados seis anticorpos monoclonais (humano, suíno, cão, bovino, rato e galinha) e destes, apenas o anti- IgG bovino apresentou instabilidade. Obteve-se 55,7% (519/932) dos repastos positivos para sangue humano, o que demonstra, a preferência alimentar destes anofelinos por humanos. Dos 206 mosquitos que apresentaram repasto único, 27,6% foi em humanos. O Anopheles darlingi apresentou 41% dos repastos em humanos e o Índice de Sangue Humano (HBI) no intradomicílio foi de 0,71. An. marajoara apresentou 51,3% dos repastos em humanos, embora tenha sido encontrada em grande quantidade no ambiente extradomiciliar e apresentando HBI no intradomicílio de 0,76. O An. nuneztovari foi a espécie mais abundante, apresentando comportamento exofílico e antropofílico, com 53,8% de repastos em humano e HBI no intradomicílio de 0,65. O método ELISA
Sanduíche está implantado, identificando a fonte alimentar das espécies de anofelinos coletadas em campo, exceto para bovino. É a primeira vez que esta técnica é utilizada
para determinação de repastos sanguíneos em anofelinos na região Amazônica
brasileira. É importante a determinação da fonte alimentar das espécies de anofelinos no
sentido de caracterizar o comportamento antropofílico e assim associá-las ou não à
transmissão de malária. / The immunoenzymatic assay for the bloodmeal identification has specificity till genus
level, sensitivity for the identification of partial bloodmeal and detection of multi
bloodmeal. It was detected the blood source of 82% of all mosquitoes specimens
collected at the transmission area. We had testes six monoclonal antibodies for human,
pig, dog, bovine, rat and chicken, and from them, only the bovine one had been
unstable. It was obtained 55,7% (519/932) of the blood source for human, which
demonstrated, these mosquitoes preference for human blood. From the 206 mosquitoes
that had just a unique blood source, 27,6% were in human. The Anopheles darlingi had
fed in human (41%) and his human bloodmeal index (HBI) indoor was 0,71. An.
marajoara had presented 51,3% of the bloodmeal in humans, although had been
collected in large amount outdoor and showed the HBI indoor of 0,76. The An.
nuneztovari was the specie more abundant, demonstrating an exophilic and
anthropophilic behavior, with 53,8% of blood source in humans and the HBI indoor of
0,65. The ELISA test is established, identifying the blood source of the anopheline
mosquitoes collected in the malaria transmission area, except for bovine. This is the first
time that this technique is being used for the determination of the blood source in
anopheline mosquitoes in Brazilian Amazonia. It is very important the determination of
the blood source of the anopheline species in order to characterize their anthropophilic
behavior and to associate them or not with the malaria transmission.
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