• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 12
  • 2
  • 2
  • Tagged with
  • 24
  • 24
  • 7
  • 6
  • 6
  • 6
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Optimization of Time-Resolved Raman Spectroscopy for Multi-Point In-Situ Photon Counting

Yu-chung Lin (11184699) 26 July 2021 (has links)
<div><p><br></p></div><p>This study makes use of a Time-Resolved Raman Spectroscopy (TRRS) system developed in the Purdue Civil Engineering spectroscopy laboratory to advance technology critical to enable field deployment of Raman spectroscopic systems, with a primary focus on developing solutions to overcome two specific barriers to Raman analysis in the natural environment: (1) obtaining Raman spectra of chemical compounds at field-relevant concentrations, and (2) realizing economical spatial monitoring. To inform both streams of activity, this work first explores the role of component choice and apparatus design on Raman system output. A component-level Raman system transfer function is developed in terms of intensity, wavelength, and time which yields detailed insight into system performance that greatly exceeds traditional single “system factor” treatments of apparatus effects. The modelling frame provided by the transfer function is universally applicable in that it is inclusive of the majority of component choices that may be encountered in any open-path or closed-path Raman system, and is likely to be valuable in efforts to assess the performance benefits and limitations of system designs, modify or tailor apparatus layouts, facilitate experiment design, and compare results obtained on different systems. </p><p><br></p><p>The system characterization offered by the transfer function is then employed to develop a multi-photon counting algorithm realized through digital signal processing (DSP) which captures photon arrivals traditionally ignored in conventional counting methods. This approach increases acquired Raman intensity for any given analyte by using detector output voltage or a voltage-time product as an energy proxy – an approach that is likey broadly applicable to any spectroscopic techniques employing detectors that make use of the photoelectric effect. In experiments carried out on analytes (nitrate, isopropanol, and rhodamine 6G) in aqueous solutions, enhanced observations enabled by the multi-photon counting algorithm are shown to increase observed Raman intensities of low Raman-yield solutions 2.0-3.1-fold compared to single-threshold analysis, and also extend the upper observation limit of strong Raman-yield solutions that would traditionally saturate detectors using a binary photon counting scheme. Notably, the improved performance offered by the multi-photon counting algorithm is realized through comparison of multi-photon and conventional counting algorithms applied to the same data in a post-processing exercise, thus eliminating any effects of test-to-test variation on results, and highlighting the ability to employ the developed counting approach without modification of traditional systems.</p><p><br></p><p>Additional insights from the system transfer function are also used to inform exploration of a novel approach to enable spatial environmental monitoring via Raman spectroscopy by combining fiber optics, optical switch technology, and the Raman system prototype. Tests designed to evaluate the system configured as a multiplexed optically switched fiber optic network demonstrate the potential to deliver excitation and collect Raman scattering from different desired monitoring locations with a sole excitation source and a single detector over substantial distances. Using nitrate as an example compound of interest, it is demonstrated that the system has a detection limit of 5 ppm within approximately 1.5 meters, which increases to 15 ppm at 100 m, and 38 ppm at 200 m. Modelling informed using the developed system transfer function highlights that improving the prototype by eliminating fiber connectors and making use of commercially available visible-light optimized fiber can substantially extend the range of the system, offering a 15-ppm nitrate detection limit at 2100 m. As increases in laser power, testing time, and collection optic efficiency are all also straightforward and viable, the prototype demonstrates realistic potential to achieve field relevant detection sensitivity over great distance.</p><p><br></p><p>As a final demonstration of system potential, a set of experiments on aqueous nitrate solutions is performed to understand the influence of turbidity, fluorescence, optics size, and varied raw data integration lengths on Raman observations. Results demonstrate that cumulative advances in the TRRS system establish a new generation of Raman spectroscopic sensing amenable to long-term environmental monitoring over significant spatial extent in complex in-situ conditions. Specific advances made herein include enhanced power delivery and scattered light collection informed by the system transfer function, increases in sensitivity from multi-photon counting, and incorporation of optical multiplexing. Overall, the Time-Resolved Raman Spectroscopic System (TRRS) now offers a set of capabilities that bring in-field deployment within practical reach.</p>
22

In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging

Freeman, Kim Renee 25 February 2014 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / The sympathetic nervous system strongly modulates the contractile and electrical function of the heart. The anatomical underpinnings that enable a spatially and temporally coordinated dissemination of sympathetic signals within the cardiac tissue are only incompletely characterized. In this work we took the first step of unraveling the in situ 3D microarchitecture of the cardiac sympathetic nervous system. Using a combination of two-photon excitation fluorescence microscopy and computer-assisted image analyses, we reconstructed the sympathetic network in a portion of the left ventricular epicardium from adult transgenic mice expressing a fluorescent reporter protein in all peripheral sympathetic neurons. The reconstruction revealed several organizational principles of the local sympathetic tree that synergize to enable a coordinated and efficient signal transfer to the target tissue. First, synaptic boutons are aligned with high density along much of axon-cell contacts. Second, axon segments are oriented parallel to the main, i.e., longitudinal, axes of their apposed cardiomyocytes, optimizing the frequency of transmitter release sites per axon/per cardiomyocyte. Third, the local network was partitioned into branched and/or looped sub-trees which extended both radially and tangentially through the image volume. Fourth, sub-trees arrange to not much overlap, giving rise to multiple annexed innervation domains of variable complexity and configuration. The sympathetic network in the epicardial border zone of a chronic myocardial infarction was observed to undergo substantive remodeling, which included almost complete loss of fibers at depths >10 µm from the surface, spatially heterogeneous gain of axons, irregularly shaped synaptic boutons, and formation of axonal plexuses composed of nested loops of variable length. In conclusion, we provide, to the best of our knowledge, the first in situ 3D reconstruction of the local cardiac sympathetic network in normal and injured mammalian myocardium. Mapping the sympathetic network connectivity will aid in elucidating its role in sympathetic signal transmisson and processing.
23

Roles of PMCA Isoforms in Ca<sup>2+</sup>-Homeostasis and Contractility of Bladder Smooth Muscle: Evidence from PMCA Gene-Ablated Mice

Liu, Li 27 June 2007 (has links)
No description available.
24

Détermination des coefficients d'ionisation de matériaux à grand gap par génération multi-photonique / Determination of the ionization rates of wide bandgap semiconductors using multi-photon generation process

Hamad, Hassan 28 April 2015 (has links)
L’utilisation des semi-conducteurs à large bande interdite (wide bandgap ou WBG) tels que le carbure de silicium SiC, le nitrure de gallium GaN, le diamant, etc… s’est répandue dans le domaine de l’électronique de puissance ces dernières décennies. Leurs caractéristiques électroniques et mécaniques font des WBGs des solutions alternatives pour remplacer le traditionnel silicium. Cependant, des études supplémentaires sont indispensables pour améliorer la tenue en tension, les pertes statiques et dynamiques et les performances en fonctionnement à haute température des composants WBGs. Dans ce cadre, deux bancs expérimentaux OBIC (Optical Beam Induced Current) spécifiques « en cours de développement » sont mis en place pendant cette thèse. L’OBIC consiste à éclairer avec un faisceau laser de longueur d’onde appropriée une jonction polarisée en inverse, des porteurs de charge sont alors créés par absorption photonique. On peut alors mesurer un courant induit par faisceau optique (OBIC) lorsque les porteurs sont générés dans la zone de charge d’espace. Après une première phase de préparation et d’adaptation de l’environnement expérimental, des essais ont mené à la démonstration du principe de génération multi-photonique en éclairant une jonction SiC avec un faisceau vert (532 nm). L’analyse des différentes mesures OBIC nous a permis de construire une image du champ électrique à la surface de la diode : une analyse non destructive pour étudier l’efficacité des protections périphériques des jonctions et pour détecter les défauts dans la structure cristalline. Egalement, la durée de vie des porteurs minoritaires a été déduite par l’analyse de la décroissance du courant OBIC au bord de la jonction. Les coefficients d’ionisation sont également déterminés par la méthode OBIC, ces coefficients sont des paramètres clés pour la prévision de la tension de claquage des composants. Nous avons réalisé des mesures OBIC dans le GaN, et nous avons observé un effet d’absorption bi-photonique dans le diamant avec un faisceau UV (349 nm). / In the last few decades, the use of wide bandgap (WBG) semiconductors (silicon carbide SiC, gallium nitride GaN, diamond, etc…) has become popular in the domain of power electronics. Their electronic and mechanical characteristics made of the WBGs a good alternative to the traditional silicon. However, additional studies are mandatory to improve the breakdown voltage, static and dynamic losses, and the performance at high temperature of the WBG devices. In this context, two specific experimental benches OBIC (Optical Beam Induced Current) -under development- are set up during this thesis. OBIC method consists to generate free charge carriers in a reverse biased junction by illuminating the device with an appropriate wavelength. An OBIC signal is measured if the charge carriers are generated in the space charge region. After a first phase of preparation and adaptation of the experimental environment, OBIC measurements led to demonstrate the multi-photonic generation by illuminating a SiC junction with a green laser (532 nm). OBIC measurements allowed giving an image of the electric field at the surface of the diode: OBIC presents a non-destructive analysis to study the efficiency of the peripheral protection and to detect the defects in the semi-conductor. Minority carrier lifetime was also deduced by studying the OBIC decrease at the edge of the space charge region. Ionization rates were extracted using OBIC method; these coefficients are key parameters to predict the breakdown voltage of the devices. OBIC measurements were also realized on the GaN, and two-photon generation was highlighted by measuring an OBIC current in the diamond when illuminating it with a UV laser beam (349 nm).

Page generated in 0.0516 seconds