Cancer remains one of the most pressing issues of contemporary science and medicine. Incidence of malignant diseases is rising worldwide and they represent a major problem for the society due to both economic and ethical issues they cause. Although the progress in cancer biology, therapy and immunology has led to the introduction of many novel therapeutic protocols, approaches and drugs with specificity defined on a molecular level into clinical practice, many malignancies retain their poor prognosis. Therefore, intense research into new ways to increase our therapeutic options is warranted. Unfortunately, bringing a completely novel drug into clinical use takes extremely high amounts of time and money and entails a high risk of failure. Therefore, a promising approach has been recently adopted which lies in repurposing compounds already used in human medicine for cancer treatment. This form of research can advance through clinical trials for a new indication much easier, faster and cheaper than researching completely new drugs. The aim of this study was to examine the anticancer potential of one such drug, mebendazole. An anthelminthic from the family of benzimidazoles, mebendazole has been in common clinical use from the 1970s and is marked by its low toxicity as well as its very low solubility....

Univerzita Karlova v Praze Přírodovědecká fakulta Studijní program: Molekulární biologie, genetika a virologie Bc. Jan Röslein Mutační a substituční tempo u sexuálních a klonáních forem: možný klíč k vysvětlení persistence sexu u modelové skupiny sekavců Mutation AND substitution rates in sexual and asexual forms: a clue to the persistence of sex in a model group of Cobitis? Typ závěrečné práce Diplomová Vedoucí závěrečné práce: Mgr. Karel Janko, Ph.D. Praha, 2015 Velký dík náleží mému školiteli Mgr. Karlu Jankovi, Ph.D. za velmi nápomocné, direktivní vedení práce. Též bych rád poděkoval panu Mgr. Janu Pačesovi, Ph.D. za více než vzdělávací rozměr v oblasti bioinformatické analýzy a Mgr. Ladislavu Pekárikovi, Ph.D., Mgr. Janu Kočímu za pomoc při analýze vybraných kapitol. Také bych rád poděkoval rodině za podporu. Všem participantům na této diplomové práci se hluboce omlouvám za způsobenou psychickou újmu. Prohlášení: Prohlašuji, že jsem závěrečnou práci zpracoval/a samostatně a že jsem uvedl/a všechny použité informační zdroje a literaturu. Tato práce ani její podstatná část nebyla předložena k získání jiného nebo stejného akademického titulu. V Praze dne 12. 8. 2015 Podpis: Abstrakt Klíčová slova: Abstract Key words: Obsah 1...

This diploma thesis provides an overview of gene conversion, its role in the pathogenesis of human diseases and the use of methods based on next-generation sequencing (NGS) for detection rare variants of DNA sequence. Labeling of target DNA molecules by random nucleotides in primer and NGS were used for detection point mutations arising de novo in the β-glucocerebrosidase gene by gene conversion between it and its pseudogene in meiotic and mitotic cells of control subjects. Primers specific for the active gene were used to selectively amplify the ninth and tenth exon of the gene where "recombinant" variants occur most frequently. Sequences generated from 20 genomic DNA samples on Illumina MiSeq platform were quality filtered, sorted by unique labels and consensus sequences were created from alignments of sequences carrying the same DNA tag. The number of potential point mutations in the samples ranged between 12 and 48. The mutations were manually re-evaluated from the alignments. The number of alignments with unique labeling was in the range of 7-15 thousand per sample. Only three samples carried possible recombinant mutations, suggesting a lower frequency of conversion in the region than reported by other techniques. Analysis of unique sequences in primer indicated possible ways to improve the...

This thesis is dedicated to a research of the influence of information technologies in Health Education in scope of various forms of education. The theoretical part focuses on determining the aims of education, teaching approaches and means of education in various areas of education. It deals with formal, non formal and informal education. A major role in this work is given to curators whose influence penetrates all spheres of teaching, especially in non formal and informal environment. This thesis shows how much the role of a teacher shifts towards curatorship and thus increases the influence it has on the target group (pupils) in an after-school environment. The empirical part is dedicated to the individual curators and video interviews with them. The questions of the interview focus on healthy life style, teaching, their presentation online and the role of technology in contemporary health education. A questionnaire researching the experience of both, pedagogues and students of the health education major, with using digital technologies in class is also a part of the empirical unit. Based on the analysis of the interviews and the questionnaire, this thesis shows the present state of the use of technologies in health education and concludes the most likely course of development. Keywords health,...

Diploma thesis Position of Germany in the context of current economic problems of European Union is focused on characteristic of status of economy and competitiveness of Germany. This analysis will lead to the most important challenges, which will contribute in the development of current German economy and its linkage to economy of European Union. The first chapter is focused on current status of German economy from the view of main economic indicators like analysis of economic power of population, gross domestic product and business environment. The second chapter pays attention to analysis of competitiveness from the view of labour market, market of goods and services and investment position of Germany. The third chapter is starting point for the previous chapters and determines the main challenges of German economy in presence which are gaining importance to the future not only of Germany, but also of European Union. The final part of my thesis is focused on possible solutions of these challenges, that can help not only to the German economy, but also to the economy of European Union to become one of the most competitive economy of the world.

The aim of diploma thesis is to suggest a mobile application for clothing stores. At the same time it takes into account how consumers and businesses are influenced by digital technologies. The app´s name is Digital Closet and its purpose is to organize your closet. Thesis includes a description of a mobile application in general and the well-known operation systems. Then one of the operation systems was set as a basic requirement for suggested application. This thesis also in part analyzes mobile applications at fashion industry. In conclusion, two surveys were conducted. First survey aimed at consumer market and the second one at clothing stores.

The thesis "New forms of Commercial communications and their use in the Creative industries" is focused on two phenomena of our time. The new forms of commercial communication like response to the abandonment of traditional communication methods, channels and media. The second phenomenon is the creative industries as an opportunity for the new economy. This work includes the analysis of specifics of marketing communication in the creative industries, analysis of commercial communication and its selected new forms and practical examples of their use in the creative industries. Based on these analyzes, I draw general conclusions and specific use of new forms of commercial communications in the communication of creative industries.

The thesis "Communication planning in Algotech in 2013" is practically based work with an objective to plan marketing communication of the company for 2013. The planning is based on the current company communication analysis and both communication goals and new trends in marketing communication are taken into consideration. The theoretical part is focused on substance of communication, communication process and tools of communication mix. In the practical part, there is the introduction of company Algotech, analysis of its communication strategy and proposal of communication plan for 2013 inclusive recommendation to utilize new trends in marketing communication and also the evaluation of executed marketing communication plan.

The growing world population accompanied by an increase in GDP is effectively raising the demand for energy. One of the options are unconventional means of oil and gas, originating mainly from shales and oil sands. The goal of this thesis is to introduce the reader to unconventional means and their influence on international business. After a short introduction and definition an analysis of the situation in the U.S. follows, where the recent years have brought an increase in production from unconventional sources. The positive influence on the economy, employment and energetic security will be mentioned same as possible impacts on the environment. Furthermore the paper provides an analysis of the potential of repeating the U.S. shale revolution in other countries, with special focus on the EU. The success of unconventional sources opens the possibility of new trade routes, influence on the trade balance and the potential of influencing the whole gas market. However, it will also have an impact on current oil and gas producers. Considering the close bond between energetic security and politics, the thesis closes with an analysis of influences on the economy and politics of both -- current oil and gas producers and countries, which might be directly and indirectly affected by changes in the oil and gas market.

1 Název diplomové práce v anglickém jazyce, abstrakt v anglickém jazyce a tři klíčová slova v anglickém jazyce Legal aspects of New Religious Movements in the Czech Republic Abstract The main purpose of this master's thesis is a critical analysis of Czech legislation of New Religious Movements. This thesis should describe a legal relationship between New Religious Movements and Czech legal order. This thesis focuses on the topic while studying public and private law's aspects. It also focuses on sociological considerations. New Religious Movements are a necessary part of a religious development of every society. There is a lot of different religious movements. Therefore, it is especially challenging to make legislation related to them. The legislator should consider religion a private part of everyone's life. Act No. 3/2002 Coll., which is the most important piece of legislation for New Religious Movements, does not distinguish terminologically between New Religious Movements and the traditional churches. A long tradition in the Czech Republic and a lot of members have for example Seventh-day Adventist Church, Jehovah's Witnesses and The Church of Jesus Christ of Latter-day Saints. Being a member of a New Religious Movement can particularly affect man's life. Conflicts between New Religious Movements and...