Thesis (Ph. D.)--Joint Program in Chemical Oceanography (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), February 2001. / Includes bibliographical references. / Processes that enable marine phytoplankton to acquire trace metals are fundamental to our understanding of primary productivity and global carbon cycling. This thesis explored the biogeochemistry of cobalt using analytical chemistry and physiological experiments with the dominant phytoplankton species, Prochlorococcus. A high sensitivity method for Co speciation was developed using hanging mercury drop cathodic stripping voltammetry. Dissolved Co at the Bermuda Atlantic Time Series station (BATS) in the Sargasso Sea was bound by strong organic complexes with a conditional stability constant of logK=16.3+0.9. A depth profile of Co at BATS revealed a nutrient-like profile. Biweekly time series measurements of total cobalt near Bermuda from the MITESS sampler were 0-47pM throughout 1999, and averaged 20±1 OpM in 1999. A transect of total cobalt from BATS to American coastal waters ranged from 19- 133pM and correlated negatively with salinity (r2=0.93), suggestive of coastal waters as an input source. Prochlorococcus strains MED4-Ax and SS120 showed an absolute requirement for Co, despite replete Zn. 57Co uptake rates and growth rates were enhanced by additions of filtered low Co cultures, suggesting that a ligand is present that facilitates Co uptake. Bottle incubations from a Synechococcus bloom in the Pacific showed production of 425pM strong cobalt ligand. These and other lines of evidence support the hypothesis that a cobalt ligand, or cobalophore, is involved in cobalt uptake. Co-limited Prochlorococcus cultures exhibited an increase in the fraction of cells in G2 relative to other cell cycle stages during exponential growth, and the durations of this stage increased with decreasing cobalt concentrations. This effect was not observed with Fe, N, or P-limited cultures, suggestive of a specific biochemical function of cobalt that would interfere with the late stages of the cell cycle. The ligand Teta was explored as a means to induce cobalt limitation. The CoTeta complex was not bioavailable to the Sargasso Sea microbial assemblage in short-term experiments. Bottle incubations with Teta did not induce cobalt limitation of Prochlorococcus. These results are consistent with the lower conditional stability constant for CoTeta (logK= 11.2+0.1) relative to natural cobalt ligands in seawater, and with culture studies that suggest uptake of cobalt via strong organic ligands. / by Mak A. Saito. / Ph.D.

Thesis (Ph.D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences and the Woods Hole Oceanographic Institution), 2000. / Vita. / Includes bibliographical references (leaves 179-187). / A spectral camera (ALISS - Ambient Light Imaging and Spectral System) was used to image ambient light from high-temperature vents at 9°N East Pacific Rise and the Juan de Fuca Ridge during 1997 and 1998 Alvin dive cruises. ALISS is a low-light digital camera with custom-designed optics. A set of nine lenses, each covered by an individual bandpass filter (50 and 100 nm nominal bandwidths), allows vents to be imaged in nine wavelength bands simultaneously spanning the range of 400-1000 nm. Thus, both spatial and spectral information are obtained. ALISS was used to image three types of vents: black smokers, flange pools, and beehives. The primary source of light is thermal radiation due to the high temperature of the hydrothermal fluid (~350°C). This light is dominant at wavelengths greater than 700 nm. At flange pools, where the fluid is relatively stable, only thermal radiation is present. Black smokers and beehives, however, are subject to mixing with ambient seawater (2°C) leading to mineral precipitation. Data from these types of vents show the existence of non-thermal, temporally varying light in the 400-700 nm region. This light is probably caused by mechanisms related to mixing and precipitation, such as chemiluminescence, crystalloluminescence and triboluminescence. / by Sheri N. White. / Ph.D.

Thesis: Ph. D., Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Department of Biology; and the Woods Hole Oceanographic Institution), 2015. / Cataloged from PDF version of thesis. / Includes bibliographical references. / Males and females may differ in stage-specific survival, maturation, fertility, or mating availability. These demographic differences, in turn, affect population growth rates, equilibrium structure, and evolutionary trajectories. Models considering only a single sex cannot capture these effects, motivating the use of demographic two-sex models for sexually reproducing populations. I developed a new two-sex modeling framework that incorporates population structure and multiple life cycle processes through transition rate matrices. These models can be applied to a variety of life histories to address both ecological and evolutionary questions. Here, I apply the model to the effects of sex-biased harvest on populations with various mating systems. Demographic considerations also affect evolutionary projections. I derived matrix calculus expressions for key evolutionary quantities in my two-sex models, including the invasion fitness, selection gradient, and second derivatives of growth rates (which have many applications, including the classification of evolutionary singular strategies). I used these quantities to analyze the evolution of the primary sex ratio, under various sex- and stage-specific offspring costs and maternal conditions. Demographic two-sex models lend insight into complex, and sometimes counterintuitive, results that are not captured by models lacking population structure. These findings highlight the importance of demographic structure in ecology and evolution. / by Esther Shyu. / Ph. D.

Thesis: Ph. D., Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2016. / Cataloged from PDF version of thesis. / Includes bibliographical references (pages 165-172). / Glacial fjords form conduits between glaciers of the Greenland Ice Sheet and the North Atlantic. They are the gateways for importing oceanic heat to melt ice and for exporting meltwater into the ocean. Submarine melting in fjords has been implicated as a driver of recent glacier acceleration; however, there are no direct measurements of this melting, and little is known about the fjord processes that modulate melt rates. Combining observations, theory, and modeling, this thesis investigates the circulation, heat transport, and meltwater export in glacial fjords. While most recent studies focus on glacial buoyancy forcing, there are other drivers - e.g. tides, local wind, shelf variability - that can be important for fjord circulation. Using moored records from two major Greenlandic fjords, shelf forcing (from shelf density fluctuations) is found to dominate the fjord circulation, driving rapid exchange with the shelf and large heat content variability near the glacier. Contrary to the conventional paradigm, these flows mask any glacier-driven circulation in the non-summer months. During the summer, when shelf forcing is reduced and freshwater forcing peaks, a mean exchange flow transports warm Atlantic-origin water towards the glacier and exports glacial meltwater. Many recent studies have inferred submarine melt rates from oceanic heat transport, but the fjord budgets that underlie this method have been overlooked. Building on estuarine studies of salt fluxes, this thesis presents a new framework for assessing glacial fjord budgets and revised equations for inferring meltwater fluxes. Two different seasonal regimes are found in the heat/salt budgets for Sermilik Fjord, and the results provide the first time-series of submarine meltwater and subglacial discharge fluxes into a glacial fjord. Finally, building on the observations, ROMS numerical simulations and two analytical models are used to investigate the dynamics of shelf-driven flows and their importance relative to local wind forcing across the parameter space of Greenland's fjords. The fjord response is found to vary primarily with the width relative to the deformation radius and the fjord adjustment timescale relative to the forcing timescale. Understanding these modes of circulation is a step towards accurate modeling of ocean-glacier interactions. / by Rebecca H. Jackson. / Ph. D.

Thesis: Ph. D., Joint Program in Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Department of Biology; and the Woods Hole Oceanographic Institution), 2016. / Cataloged from PDF version of thesis. / Includes bibliographical references (pages 124-131). / Dinoflagellates are a diverse group of single-celled eukaryotic phytoplankton that are important for their unique genetics and molecular biology, the multitude of ecological roles they play, and the ability of multiple species to produce toxins that affect human and ecosystems health. Two dinoflagellate genera, Alexandrium and Gambierdiscus each contain species that can cause human poisoning syndromes, although the methods of toxin transfer, accumulation, and exposure are very different. Gambierdiscus is a benthic organism that produces lipophilic ciguatoxins that can bioaccumulate in coral reef fish and cause ciguatera fish poisoning (CFP) in human consumers. Alexandrium is a planktonic species that produces saxitoxins that can directly accumulate in shellfish and cause paralytic shellfish poisoning (PSP) in humans. Both genera contain multiple species that vary dramatically in toxicity and physiology. Through transcriptomic analysis, this thesis describes the genetic diversity present across dinoflagellates that produce saxitoxin, elaborating on differences in their complement of genes within the saxitoxin biosynthesis pathway. This study demonstrated retention and expression of some of these saxitoxin genes by non-toxic species within Alexandrium, as well as in Gambierdiscus, which does not produce saxitoxins. Furthermore it confirmed the presence of certain transcripts only in toxin-producing species. This thesis then developed novel fluorescence in situ hybridization (FISH) probes that can be used to identify and enumerate six Gambierdiscus species, thereby enabling the community composition of Gambierdiscus to be examined in a quantifiable manner. The probes were tested in the laboratory on cultures, and then successfully applied to field samples from Florida Keys and Hawai'i. Gambierdiscus species are diverse in both their toxicity and optimal temperature ranges for growth. Analysis of Gambierdiscus community composition in an area of variable temperature allowed the characterization of species shifts that were driven both by a seasonal increase in mean seawater temperatures and spatial variability of temperature experienced between tidal pools. Overall this thesis advances the knowledge of dinoflagellate genetics and ecology, aids in the characterization of species harmful to public health, and provides tools and approaches to help monitor and manage harmful effects from these species, including some that are projected to increase with climate change. / by Kathleen Johnson Pitz. / Ph. D.

Thesis: Ph. D., Joint Program in Chemical Oceanography (Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2017. / Cataloged from PDF version of thesis. / Includes bibliographical references. / Particulate organic carbon (POC) in the ocean and mobilized by rivers on land transfers -0. 1% of global primary productivity to the deep ocean sediments. This small fraction regulates the long-term carbon cycle by removing carbon dioxide from the atmosphere for centuries to millennia. This thesis investigates mechanisms of POC transfer to the deep ocean by analyzing particles collected in transit through two globally significant carbon reservoirs: the Southern Ocean and the Amazon River Basin. These endeavors test the hypothesis that organic matter composition controls the recycling and transfer efficiency of POC to the deep ocean, and illustrate new applications for ramped pyrolysis/oxidation (RPO), a growing method of POC characterization by thermal stability. By coupling RPO to stable and radiocarbon isotope analyses of riverine POC, I quantify three thermally distinct soil organic carbon pools mobilized by the Amazon River, and evaluate the degradability and fate of these different pools during transport to the coastal Atlantic Ocean. More directly, RPO analyses of marine samples suggest that POC transfer in the water column is in fact selective. Observations of consistent biomolecular changes that accompany transport of phytoplankton-derived organic matter to depth across the Southern Ocean support the argument for preferential degradation of specific POC pools in the water column. Combining discussions of POC recycling and transfer across both marine and terrestrial systems offer new perspectives of thermal stability as a proxy for diagenetic stability and POC degradation state. The challenges of interpreting RPO data in these two environments set the stage for applying the technique to more controlled experiments that trace POC from source to long-term sink. / by Sarah Zhou Rosengard. / Ph. D.

Thesis (Ph. D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2008. / Includes bibliographical references (p. 135-140). / Chapter 1 presents the first published measurements of Sr-isotope variability in olivine-hosted melt inclusions. Melt inclusions in just two Samoan basalt hand samples exhibit most of the total Sr-isotope variability observed in Samoan lavas. Chapter 3 deals with the largest possible scales of mantle heterogeneity, and presents the highest magmatic 3He/4He (33.8 times atmospheric) discovered in Samoa and the southern hemisphere. Along with Samoa, the highest 3He/4He sample from each southern hemisphere high 3He/4He hotspot exhibits lower 143Nd/144Nd ratios than their counterparts in the northern hemisphere. Chapter 2 presents geochemical data for a suite of unusually enriched Samoan lavas. These highly enriched Samoan lavas have the highest 87Sr/86Sr values (0.72163) measured in oceanic hotspot lavas to date, and along with trace element ratios (low Ce/Pb and Nb/U ratios), provide a strong case for ancient recycled sediment in the Samoan mantle. Chapter 4 explores whether the eclogitic and peridotitic portions of ancient subducted oceanic plates can explain the anomalous titanium, tantalum and niobium (TITAN) enrichment in high 3He/4He ocean island basalts (OIBs). The peridotitic portion of ancient subducted plates can contribute high 3He/4He and, after processing in subduction zones, a refractory, rutile-bearing eclogite may contribute the positive TITAN anomalies. / by Matthew G. Jackson. / Ph.D.

Thesis (Ph. D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2008. / Includes bibliographical references. / Segmentation and crustal accretion at oceanic transform fault systems are investigated through a combination of geophysical data analysis and geodynamical and geochemical modeling. Chapter 1 examines the effect of fault segmentation on the maximum predicted earthquake magnitude of an oceanic transform fault system. Results of thermal modeling suggest that fault segmentation by intra- transform spreading centers (ITSC) drastically reduces the available brittle area of a transform fault and thus limits the available earthquake rupture area. Coulomb stress models suggest that long ITSCs will prohibit static stress interaction between segments of a transform system and further limit the maximum possible magnitude of a given transform fault earthquake. In Chapter 2, gravity anomalies from a global set of oceanic transform fault systems are investigated. Surprisingly, negative residual mantle Bouguer gravity anomalies are found within fast-slipping transform fault domains. These gravity observations suggest a mass deficit within fast-slipping transform faults, which may result from porosity variations, mantle serpentinization, and/or crustal thickness variations. Two-dimensional forward modeling and the correlation of the negative gravity anomalies to bathymetric highs indicate crustal thickness excesses in these locations. Finally, in Chapter 3, mantle thermal and melting models for a visco-plastic rheology are developed to investigate the process of mantle melting and crustal accretion at ITSCs within segmented transform faults, and are applied to the Siqueiros transform fault system. Models in which melt migrates into the transform fault domain from a large region of the mantle best explain the gravity-derived crustal thickness variations observed at the Siqueiros transform. Furthermore, a mantle potential temperature of 1350⁰C and fractional crystallization at depths of 9 - 15.5 km best explain the major element composition variation observed at the Siqueiros transform. / by Patricia Michelle Marie Gregg. / Ph.D.

Thesis (M.S.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), February 1988. / "October 1987." / Includes bibliographical references (leaves 87-89). / Thermohaline staircases consisting of a series of well mixed layers approximately 30 m thick are found at depths of 300-500 m in a region of the tropical North Atlantic spanning 48° to 58°W, 8° to 17° N. Density ratios ( ... ) with values near 1 indicate a double diffusive origin for the structure (Schmitt, 1981,1986). Determining the importance of double-diffusive mixing to the regional advection - diffusion balance is the subject of this study. Using hydrographic and current meter data collected in the C-SALT program of 1985 (Schmitt, 1987), we construct inverse models in both cartesian and density coordinates and seek bounds on the cross isopycnal mixing in the staircase region. In cartesian coordinates, the role of diffusion was not well resolved, probably due to inadequacy of the steady state model in the presence of eddies. By reformulating the problem in boxes bounded by isopycnals which more closely follow the layers, and solving directly for cross isopycnal fluxes of salt and heat, the resolution of the diffusivities was improved. Inversions were done on data from the spring and fall CTD surveys. The average salt diffusivity in the step region was estimated from the isopycnal inversions to be ks = (2.8 + 1.3)cm2 /s in spring and ks = (3.9 ± 2.2)cm2 /s in fall. / by Joyce Marie Federiuk. / M.S.

Thesis: Ph. D., Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Department of Mechanical Engineering; and the Woods Hole Oceanographic Institution), June 2014. / Cataloged from PDF version of thesis. "June 2014." / Includes bibliographical references (pages 103-112). / Evolutionary pressures have led to some astonishing camouflage strategies in the animal kingdom. Cephalopods like cuttlefish and octopus mastered a rather unique skill: they can rapidly adapt the way their skin looks in color, texture and pattern, blending in with their backgrounds. Showing a general resemblance to a visual background is one of the many camouflage strategies used in nature. For animals like cuttlefish that can dynamically change the way they look, we would like to be able to determine which camouflage strategy a given pattern serves. For example, does an inexact match to a particular background mean the animal has physiological limitations to the patterns it can show, or is it employing a different camouflage strategy (e.g., disrupting its outline)? This thesis uses a computational and data-driven approach to quantify camouflage patterns of cuttlefish in terms of color and pattern. First, we assess the color match of cuttlefish to the features in its natural background in the eyes of its predators. Then, we study overall body patterns to discover relationships and limitations between chromatic components. To facilitate repeatability of our work by others, we also explore ways for unbiased data acquisition using consumer cameras and conventional spectrometers, which are optical imaging instruments most commonly used in studies of animal coloration and camouflage. This thesis makes the following contributions: (1) Proposes a methodology for scene-specific color calibration for the use of RGB cameras for accurate and consistent data acquisition. (2) Introduces an equation relating the numerical aperture and diameter of the optical fiber of a spectrometer to measurement distance and angle, quantifying the degree of spectral contamination. (3) Presents the first study assessing the color match of cuttlefish (S. officinalis) to its background using in situ spectrometry. (4) Develops a computational approach to pattern quantification using techniques from computer vision, image processing, statistics and pattern recognition; and introduces Cuttlefish 72x5, the first database of calibrated raw (linear) images of cuttlefish. / by Derya Akkaynak. / Ph. D.