The PhD is concerned with the construction of site knowledge and how this is transformed into knowing where and how to intervene in a river system close to ecological collapse. It involves three overlapping topics: • Site knowledge and its impact upon the design process • Development of tools and techniques appropriate for working on a particular type of site condition: the threshold between land and water • Transitory: the impact of dynamic processes and events on inhabitation Site knowledge emerges from a process of investigating a location. It is generated by on-site and off-site operations. This involves the architect in a dynamic set of relationships - between encounters on the ground in the here and now, with more remote encounters with the site from the studio and archive. This mode of site study amplifies the impact of scale shift and it exposes the variable and provisional status of a location, while also providing a way of operating in environments that can be considered dynamic. The PhD is premised upon the need for a work to relate to its surrounding environment. The hinged meaning between the terms a site and to site have relevance to the design process. A site, as a noun, suggests a specific place, such as a plot of land, whereas the verb to site, suggests that a work will be placed in relation to other things. Site knowledge is thus generated through the act of describing a place, through the act of making drawings and other descriptions of that place. It generates ways of conceptualising a site and leads to action: knowing how and where to intervene in a location. The River Murray provided a context for the project work of the PhD. Research led to tools for recording (on site) and interpreting (off site) the impacts of flood events on the settlements on the riverbanks that were protected by levees that worked against the natural forces of the system. The research culminated in a range of designs that demonstrated how to integrate town and tourist developments into the re-established cyclical flows necessary for the health of the system.

The inevitable changes to the built environment over time presents the question of what contemporary design is appropriate for existing historical context. This is inherently a wicked problem that is becoming increasingly important to designers in the 21st century. Wicked problems, as the connotation implies, are those that are multi-faceted, unique, and with innumerable possible solutions (Rittel, Webber 1973). Each individual architectural project is a cog in a city’s evolving machine, therefore no one project should ever be considered unimportant. As Robert Venturi said in his “Gentle Manifesto”, a designer should strive towards “messy vitality over obvious unity” (Venturi, 1966). Finding a simple design resolution is difficult if not impossible in a complex urban city layered with centuries of architecture. It is not necessary to copy the historic building next door, nor is it appropriate to design as if a site has no neighbors. The surrounding context should be evaluated for its mass, scale, program, history, and materials, among others, to inform and inspire a contemporary designer’s work. This thesis offers no “solution”; rather a series of design considerations. These considerations are by no means prescriptive, however. My aspiration is that this thesis can be used by future designers as a tool to prompt discussion and discovery about their own site specific project. / Master of Architecture

This research proposes a new site analysis methodology in the form of an integrated framework. The framework separates the site analysis process into different models, incorporates each model, and considers the interaction between them. The most important models are the environmental models (climate, geology, hydrology, topography, and vegetation models), social-cultural models, economic models, and infrastructure models. Each model also contains several important factors. The study identifies and organizes environmental factors within the framework that influence site analysis and design. Based on the applicability of feng shui principles and their interpretations into measurable factors, this research compares and incorporates feng shui and contemporary environmental design theories, and summarizes essential environmental factors. The emphasis on environmental factors from these models may lead to a better understanding of the relationships between humankind and the natural environment. The proposed framework is implemented into a computer simulation program, titled SiteOne, to demonstrate the concepts and ideas, with an emphasis on environmental factors. This research uses the town of Reston in Fairfax County, Virginia, as the study area because of the availability of information. SiteOne analyzes a range of alternatives and then derives solutions from the suggested site conditions in the form of GIS maps. There are various modules that make up the prototype system: namely an analysis module, a database module, and a result generation module. SiteOne helps professionals collect a wide range of information and select corresponding criteria in the early stages of design. It also provides visual analysis based on selected criteria and models. Therefore, it can act as an educational knowledge component for professionals as well as the general public. / Ph. D.

The site analysis and mapping methods in the design and planning professions follow a standardized quantitative and qualitative analyis of place that favors a design process which can limit creativity and render it difficult to do anything with the normative. This work is an exploration of the development of a design approach and method that uses voice mapping as a basis for design. The voice maps contain oral histories and personal accounts of landscape experiences. Voice mapping is employed not only as a method or for site analysis but also as a generator or ideas. Voice Map Trekking is explored through a trek in the Canadian Arctic and across the Canadian Prairies. Two specific landscapes were chosen as bases for testing concepts - one near St. Gertrude SK and the other near Morinville AB. / February 2008

The site analysis and mapping methods in the design and planning professions follow a standardized quantitative and qualitative analyis of place that favors a design process which can limit creativity and render it difficult to do anything with the normative. This work is an exploration of the development of a design approach and method that uses voice mapping as a basis for design. The voice maps contain oral histories and personal accounts of landscape experiences. Voice mapping is employed not only as a method or for site analysis but also as a generator or ideas. Voice Map Trekking is explored through a trek in the Canadian Arctic and across the Canadian Prairies. Two specific landscapes were chosen as bases for testing concepts - one near St. Gertrude SK and the other near Morinville AB. / February 2008

Ion mobility spectroscopy (IMS) has been showen to provide fast on-site analysis of coarse sandy soil for the determination of polychlorinated biphenyls (PCBs). However the presence of humus results in instrument foaling and extensive down time do to instrument clean-up. For this reason a method was investigated for the ultrasonic extraction of PCBs from humus-rich soil that could be used at remote locations. Analysis of the extracted PCBs was conducted using 1) IMS and 2) gas chromatogram equipped with a dry electolytic conductiviy detector (GC/DELCD). The research conducted for this thesis outlines the method development and analysis of PCBS using these two instruments. The IMS analyiss was found to be complicated by co-extracted matrix compounds. Results and limitations of IMS analysisare present here. The method development and validation of a method for the ultrasonic extraction and analysis of PCBs using the GC/DELCD is provided.

Ion mobility spectroscopy (IMS) has been showen to provide fast on-site analysis of coarse sandy soil for the determination of polychlorinated biphenyls (PCBs). However the presence of humus results in instrument foaling and extensive down time do to instrument clean-up. For this reason a method was investigated for the ultrasonic extraction of PCBs from humus-rich soil that could be used at remote locations. Analysis of the extracted PCBs was conducted using 1) IMS and 2) gas chromatogram equipped with a dry electolytic conductiviy detector (GC/DELCD). The research conducted for this thesis outlines the method development and analysis of PCBS using these two instruments. The IMS analyiss was found to be complicated by co-extracted matrix compounds. Results and limitations of IMS analysisare present here. The method development and validation of a method for the ultrasonic extraction and analysis of PCBs using the GC/DELCD is provided.

The site analysis and mapping methods in the design and planning professions follow a standardized quantitative and qualitative analyis of place that favors a design process which can limit creativity and render it difficult to do anything with the normative. This work is an exploration of the development of a design approach and method that uses voice mapping as a basis for design. The voice maps contain oral histories and personal accounts of landscape experiences. Voice mapping is employed not only as a method or for site analysis but also as a generator or ideas. Voice Map Trekking is explored through a trek in the Canadian Arctic and across the Canadian Prairies. Two specific landscapes were chosen as bases for testing concepts - one near St. Gertrude SK and the other near Morinville AB.

1 ABSTRACT The diploma theses deals with marketing in practice of social services. Marketing brings positive effects in the field of client, personal and material-technical capacities to some organisations. This can bring financial as well as nonfinancial profit together with bigger independence from donors who provide means of running of social services. Theses will help to solve problems of some social services managers who don't care about marketing in the organisation sufficiently. The object of this theses is to choose marketing goals and strategies of their obtainment on the basis of marketing macromedium and site analysis of organisation and insert them into the marketing plan in the particular social service, which moves in the competitive setting of other social services. The theses output is marketing plan of social service. Key words: marketing, social service, competition, marketing plan, site analysis, management

Indiana University-Purdue University Indianapolis (IUPUI) / Lentiviral vector mediated gene therapy has made great strides in recent years with several successful clinical trials. However, adverse events encountered with some early trials have highlighted the necessity to improve upon its safety. Improvements can range from early steps in vector production to evaluation of insertion sites post-transduction. We have evaluated an FDA approved DNase for removal of residual plasmid DNA during vector production, developed novel non-integrating lentiviral vectors and employed modified insertion site analysis post-transduction to improve the safety of lentiviral vector mediated gene therapy. To prevent the exposure of gene therapy patients to HIV-1 DNA it is essential to remove residual plasmid DNA during vector production. We evaluated a recombinant human DNase which has been FDA approved for use in patients as an alternative to a bacterially derived DNase. Our results indicate this DNase is an effective alternative with a potentially safer profile for use in patients. The ability of lentiviral vectors to stably integrate their genome into a host cell’s DNA can have negative side-effects due to the risk of insertional mutagenesis. Non-integrating lentiviral vectors have been developed to alleviate this risk in applications where integration is not necessary. However, a low frequency of illegitimate integration persists when using these vectors. We have developed a novel non-integrating vector mutation and evaluated the efficacy of combining it with other mutations for reducing the frequency of illegitimate integration. We demonstrate that combining mutations that inhibit integration can further reduce the frequency of illegitimate integration. Several methodologies have been developed for evaluating the insertion sites of normal integrating lentiviral vectors. Illegitimate integration by non-integrating vectors demonstrates mechanisms which result in insertions and/or deletions at the vector-genome junction. Current methods lack the sensitivity to account for these variables in a high-throughput manner. We have adapted modifications to current methods to improve the capture of these variable insertion sites for analysis. The results of these studies improve the safety of lentiviral vector mediated gene therapy by improving the purity of the vector product, providing a safer vector for non-integrase mediated applications, and allowing more sensitive analysis of insertion sites post-transduction.