Em Trypanosoma cruzi os sítios de replicação estão localizados na periferia nuclear. A fim de entender a dinâmica das moléculas envolvidas na replicação, Orc/Cdc6 foi usado como marcador da maquinaria de pré-replicação e PCNA como marcador da maquinaria de replicação durante o ciclo celular da forma epimastigota. Ambas as moléculas apresentaram dois padrões de localização nuclear: padrão disperso e periférico. Em ensaio de dupla marcação três combinações dos padrões de Orc/Cdc6 e TcPCNA foram encontrados durante G1/S: Orc/Cdc6 periférico e PCNA disperso, ambos dispersos e ambos periféricos. Por meio destes resultados pudemos concluir que durante G1 ambas as moléculas se encontram dispersas. Ao final desta fase, Orc/Cdc6 migra para periferia nuclear enquanto PCNA permanece disperso, migrando para a periferia nuclear quando a célula entra em S, quando a replicação do DNA irá ocorrer. Assim, a replicação na periferia nuclear não se deve à localização prévia das moléculas de replicação nesta região, mas sim à migração destas moléculas para os sítios apropriados. / In Trypanosoma cruzi the replication sites are located at nuclear periphery. In order to analyse the dynamics of molecules involved in replication, Orc/Cdc6 was used as a marker of pre-replication machinery and PCNA as a marker of replication machinery during the cell cycle of epimastigote form. Both molecules presented two nuclear patterns: dispersed pattern and peripheral pattern. Double-labeling assay showed three different patterns of Orc/Cdc6 and PCNA in the nucleus: Orc/Cdc6 at nuclear periphery and PCNA dispersed, both dispersed and both at nuclear periphery. This data allowed us to conclude that during early G1 phase both molecules are dispersed in the nucleus and during late G1 Orc/Cdc6 goes to nuclear periphery while TcPCNA remains dispersed, moving to nuclear periphery, where DNA replication will take place, when S phase starts. Thus, the replication of DNA at nuclear periphery is not due to localization of replications factors at nuclear periphery; instead it depends on the movement of these factors to the appropriated sites.

Em Trypanosoma cruzi os sítios de replicação estão localizados na periferia nuclear. A fim de entender a dinâmica das moléculas envolvidas na replicação, Orc/Cdc6 foi usado como marcador da maquinaria de pré-replicação e PCNA como marcador da maquinaria de replicação durante o ciclo celular da forma epimastigota. Ambas as moléculas apresentaram dois padrões de localização nuclear: padrão disperso e periférico. Em ensaio de dupla marcação três combinações dos padrões de Orc/Cdc6 e TcPCNA foram encontrados durante G1/S: Orc/Cdc6 periférico e PCNA disperso, ambos dispersos e ambos periféricos. Por meio destes resultados pudemos concluir que durante G1 ambas as moléculas se encontram dispersas. Ao final desta fase, Orc/Cdc6 migra para periferia nuclear enquanto PCNA permanece disperso, migrando para a periferia nuclear quando a célula entra em S, quando a replicação do DNA irá ocorrer. Assim, a replicação na periferia nuclear não se deve à localização prévia das moléculas de replicação nesta região, mas sim à migração destas moléculas para os sítios apropriados. / In Trypanosoma cruzi the replication sites are located at nuclear periphery. In order to analyse the dynamics of molecules involved in replication, Orc/Cdc6 was used as a marker of pre-replication machinery and PCNA as a marker of replication machinery during the cell cycle of epimastigote form. Both molecules presented two nuclear patterns: dispersed pattern and peripheral pattern. Double-labeling assay showed three different patterns of Orc/Cdc6 and PCNA in the nucleus: Orc/Cdc6 at nuclear periphery and PCNA dispersed, both dispersed and both at nuclear periphery. This data allowed us to conclude that during early G1 phase both molecules are dispersed in the nucleus and during late G1 Orc/Cdc6 goes to nuclear periphery while TcPCNA remains dispersed, moving to nuclear periphery, where DNA replication will take place, when S phase starts. Thus, the replication of DNA at nuclear periphery is not due to localization of replications factors at nuclear periphery; instead it depends on the movement of these factors to the appropriated sites.

In this masters thesis are discusses the possibilities of increasing the electrical efficiency of cogeneration units with an internal combustion engine with the power output 800 kWe. Technical and economical aspect the connection with steam turbine, steam engine and ORC turbine is compared. It include the design of heat exchanger for ORC cycle.

Increasing the efficiency of conventional power plants is a crucial aspect in the quest of reducing the energy consumption of the world and to having sustainable energy systems in the future. Thus, within the scope of this thesis the possible efficiency improvements for the Wärtsilä 18V50DF model gas engine based combine power generation options are investigated by recovering waste heat of the engine via Organic Rankine cycle (ORC).  In order to this, four different ORC models are simulated via Aspen Plus software and these models are optimized for different objective functions; power output and price per unit of electricity generation. These ORC models are: regenerative Organic Rankine cycle (RORC), cascaded Organic Rankine cycle with an economizer (CORCE), cascaded Organic Rankine cycle with two heat sources (CORC2) and cascaded Organic Rankine cycle with three heat sources (CORC3). In the cascaded cycle models there are two loops which are coupled with a common heat exchanger that works as a condenser for the high temperature (HT) loop and as a preheater for the low temperature (LT) loop. By using this common heat exchanger, the latent heat of condensation of the HT loop is utilized. The engine’s hot exhaust gases are used as main heat source in all the ORC models. The engine’s jacket water is utilized in the CORC2 models as an additional heat source to preheat the LT working fluid. In the CORC3 models engine’s lubrication oil together with the jacket water are used as additional sources for preheating the LT loop working fluid. Thus, the suitability of utilizing these two waste heat sources is examined. Moreover, thermodynamic and economic analyses are performed for each model and the results are compared to each other. The effect of different working fluids, condenser cooling water temperatures, superheating on cycles performance is also evaluated. The results show that with the same amount of fuel the power output of the engine would be increased 2200 kW in average and this increases the efficiency of the engine by 6.3 %. The highest power outputs are obtained in CORC3 models (around 2750 kW) whereas the lowest are in the RORC models (around 1800 kW). In contrast to the power output results, energetic efficiencies of the RORC models (around 30 %) are the highest and CORC3 models (around 22 %) are the lowest. In terms of exergetic efficiency, the highest efficiencies are obtained in CORC2 (around 64.5 %) models whereas the lowest in the RORC models (around 63 %). All the models are found economically feasible since thermodynamically optimized models pay the investment costs back in average of 2 years whereas the economically optimized ones in 1.7. The selection of the working fluid slightly affects the thermodynamic performance of the system since in all the ORC configurations Octamethyltrisiloxane (MDM) working fluid cycles achieve better thermodynamic performances than Decamethyltetrasiloxane (MD2M) working fluid cycles. However, the choice of working fluid doesn’t affect the costs of the system since both working fluid cycles have similar price per unit of electricity generation. The CORC2 models obtain the shortest payback times whereas the CORC3 models obtain the longest Thus the configuration of the ORC does affect the economic performance. It is observed from the results that increasing the condenser cooling water temperature have negative impact on both thermodynamic and economic performances. Also, thermodynamic performances of the cycles are getting reduced with the increasing degree of superheating thus superheating negatively affects the cycle’s performances. The engine’s jacket water and lubrication oil are found to be sufficient waste heat sources to use in the ORC models.

The aim of this thesis is to evaluate ORC systems and technologies from an energy and economic perspective. ORC systems are a growing renewable electricity generation technology, but New Zealand has limited local skills and expertise for identifying ORC resource opportunities and subsequently developing suitable technologies at low cost. For this reason, this thesis researches ORC technology, resource types, and international development, with the aim to determine guidelines for how to cost-effectively develop ORC systems, and to make recommendations applicable to furthering their development within a New Zealand context. This thesis first uses two surveys, one of commercial ORC installations, and a second of economic evaluations of ORC systems in literature, to determine what resources and economic scenarios are supportive of commercial development. It is found that geothermal resources provide the largest share of ORC capacity, with biomass and waste-heat recovery (WHR) being developed more recently. The surveys also found that countries with high electricity prices or policy interventions have developed a wider range of resources using ORC systems. This thesis then undertakes an EROI evaluation of ORC electricity generation systems using a combination of top-down and process based methodologies. Various heat sources; geothermal, biomass, solar, and waste heat are evaluated in order to determine how the utilised resource can affect energy profitability. A wide range of EROIstnd values, from 3.4 – 22.7 are found, with solar resources offering the lowest EROIs, and geothermal systems the highest. Higher still EROI values are found to be obtainable with longer system lifetimes, especially for WHR systems. Specific engineering aspects of ORC design and technology such as high-side pressure, heat storage, modularity, superheating, pinch-point temperature difference, and turbine efficiency are evaluated in terms of economic performance, and a variety of general conclusions are made about each. It is found that total system thermo-economic optimisation may not lead to the highest possible EROI, depending on the objective function. Lastly, the effects of past and potential future changes to the markets and economies surrounding ORCs are explored, including the New Zealand electricity spot price, steel and aluminium prices, subsidies, and climate policy. Of the subsidy types explored, it is found that directly subsidising ORC system capital has the greatest effect on the economic performance of ORC systems, as measured by common metrics. In conclusion, this thesis finds that ORC systems have a limited applicability to New Zealand’s electricity market under current economic conditions outside of geothermal and off-grid generation, but changes to these conditions could potentially make their development more viable. The author recommends that favourable resources should be developed using systems that provide high efficiencies, beyond what might provide the best economic performance, in order to increase EROI, and reduce the future need for costly investments into increasingly less favourable resources.

The aim of this thesis is to evaluate ORC systems and technologies from an energy and economic perspective. ORC systems are a growing renewable electricity generation technology, but New Zealand has limited local skills and expertise for identifying ORC resource opportunities and subsequently developing suitable technologies at low cost. For this reason, this thesis researches ORC technology, resource types, and international development, with the aim to determine guidelines for how to cost-effectively develop ORC systems, and to make recommendations applicable to furthering their development within a New Zealand context. This thesis first uses two surveys, one of commercial ORC installations, and a second of economic evaluations of ORC systems in literature, to determine what resources and economic scenarios are supportive of commercial development. It is found that geothermal resources provide the largest share of ORC capacity, with biomass and waste-heat recovery (WHR) being developed more recently. The surveys also found that countries with high electricity prices or policy interventions have developed a wider range of resources using ORC systems. This thesis then undertakes an EROI evaluation of ORC electricity generation systems using a combination of top-down and process based methodologies. Various heat sources; geothermal, biomass, solar, and waste heat are evaluated in order to determine how the utilised resource can affect energy profitability. A wide range of EROIstnd values, from 3.4 – 22.7 are found, with solar resources offering the lowest EROIs, and geothermal systems the highest. Higher still EROI values are found to be obtainable with longer system lifetimes, especially for WHR systems. Specific engineering aspects of ORC design and technology such as high-side pressure, heat storage, modularity, superheating, pinch-point temperature difference, and turbine efficiency are evaluated in terms of economic performance, and a variety of general conclusions are made about each. It is found that total system thermo-economic optimisation may not lead to the highest possible EROI, depending on the objective function. Lastly, the effects of past and potential future changes to the markets and economies surrounding ORCs are explored, including the New Zealand electricity spot price, steel and aluminium prices, subsidies, and climate policy. Of the subsidy types explored, it is found that directly subsidising ORC system capital has the greatest effect on the economic performance of ORC systems, as measured by common metrics. In conclusion, this thesis finds that ORC systems have a limited applicability to New Zealand’s electricity market under current economic conditions outside of geothermal and off-grid generation, but changes to these conditions could potentially make their development more viable. The author recommends that favourable resources should be developed using systems that provide high efficiencies, beyond what might provide the best economic performance, in order to increase EROI, and reduce the future need for costly investments into increasingly less favourable resources.

Dans le contexte économique et énergétique actuel, la mise en œuvre de technologies à l'aide de l'énergie renouvelable comme source de chauffage offre un double avantage: la réduction de la pollution et des coûts de carburant. Il y a un besoin de promouvoir les sources renouvelables d'énergie comme les sources significatives de production d'énergie pour les systèmes décentralisés. Une première étude bibliographique a été fait sur les technologies existantes pour la production d'énergie électrique à partir du solaire. Cette étude consiste dans la recherche d’une nouvelle solution de conversion de l’énergie solaire pour la production d’électricité de faible puissance. L'un des objectifs de cette thèse a été la construction d'un moteur Stirling de type gamma fonctionnant à basse différence de température, adapté à un circuit solaire (capteur plan). Le moteur Stirling a été testé en vue de comparer les résultats expérimentales avec les résultats d’un model Schmidt, fait dans le logiciel, Matlab. Un autre cycle thermodynamique étais étudie dans cette travail, le Cycle Organique Rankine (ORC). Un modèle mathématique a été développé et vérifie dans les logiciels, Thermoptim et EES (Engineering Equation Solver) avec les résultats expérimentaux pour étudier les performances d'installation avec des différentes températures de fonctionnement. La méthode exergétique et la méthode du Pincement sont utilisée pour évaluer les performances du système comme irréversibilité, destruction d’exergie et phénomènes qui se produisent dans toutes les composantes du système ORC pour améliorer son fonctionnement. / In the current economic and energy context, implementation of technologies using renewable energy as heat source has two advantages: reducing pollution and fuel costs. There is a need to promote renewable energy sources such as significant sources of power generation for decentralized systems. In the first part, it was made a literature review on existing technologies for the production of electricity with solar energy. One of the objectives of this thesis was to build a Stirling engine gamma type suitable to use solar energy (flat plate collator). The Stirling engine was tested to compare the experimental results with the results of Schmidt model, realized in the software, Matlab. Another thermodynamic cycle was studied in this work, the Organic Rankine Cycle (ORC). A mathematical model was developed and verified in software, Thermoptim and EES (Engineering Equation Solver) with experimental results to study the installation performance function of different operating temperatures. The entire system and each subsystem are analyzed according to the first and the second law of thermodynamics. The exergy method and Pinch analysis are used to evaluate the performance of the system like irreversibility and exergy destruction, phenomenon that occurs in all components of the ORC system. This analysis is to improve the operation.

La responsabilité de notre activité dans les récentes et parfois brutales modifications climatiques est avérée. Maîtrise de la demande en énergie et énergies renouvelables apparaissent comme les deux solutions pour remédier à cette catastrophe. Dans ce travail, nous nous intéressons à la cogénération appliquée aux bâtiments résidentiels. Deux zones géographiques sont concernées, l'Afrique de l'Ouest et la France. Il n'existe pas de système de cogénération solaire de très faible puissance (< 10 kWe). La solution envisagée dans ce travail consiste à produire de la chaleur à environ 150 °C et un rendement supérieur à 50 %, de l'utiliser ensuite dans un ORC pour produire électricité et chaleur à basse température. Le système complet doit être résistant et à bas coût. Or pour atteindre ces performances, la concentration solaire est obligatoire. Une partie de ce travail consiste donc au développement d'un panneau à concentration solaire qui répond à ces deux contraintes thermiques, mais aussi au fait d'être robuste, fiable et facilement intégrable à l'enveloppe d'un bâtiment. Dans ce cadre, la technologie cylindro-parabolique a été retenue, adaptée et miniaturisée. En ce qui concerne la partie thermodynamique, le verrou technologique se trouve principalement dans le groupe turboalternateur. L'objet de la seconde partie de cette thèse consiste ainsi à la conception d'un organe de détente également robuste, nécessitant qu'une maintenance simplifiée et réalisable par les équipes de SIREA. La turbine Tesla, brevetée en 1913 par Nikola Tesla, devrait satisfaire à ce cahier des charges. Sa particularité est qu'à l'opposée des autres turbines, son rotor ne possède pas d'aubage, mais seulement des disques parallèles. Son fonctionnement est basé sur l'adhésion du fluide aux surfaces des disques. / The responsibility of our activity in the recent and sometimes brutal climate changes is recognized. Energy demand management and renewable energies appear as two solutions to overcome this disaster. In this work, we focus on combined heat and power applied to residential buildings. Two geographical areas are concerned, West Africa and France. For the moment, no system of very low power (< 10 kWe) solar cogeneration exists. In this work, considered solution consists to produce heat at 150 °C and with an efficiency greater than 50 %, then to use it in an ORC for producing electricity and low temperature heat. The whole system has to be resistant and low-cost. But to reach those performances with solar radiation, concentration is necessary. The first part of this thesis is to elaborate a solar concentrating panel which answer to these two thermal constraints. The new solar panel must be robust, reliable and easily integrable on the building envelope. In this context, parabolic trough is adopted, adapted and miniaturised. Regarding the thermodynamic part, technological lock is found mainly in the turbogenerator. The purpose of the second part of this thesis consists of the design of a an expansion equipement, requiring simplified maintenance and achievable by the team of Sirea. The Tesla turbine, patented in 1913 by Nikola Tesla, should satisfy this specification. Its characteristic is that the opposite other conventional turbines, the rotor is not bladed or vaned, only parallel disks. Fluid exerts shear stress on the disk surfaces resulting in a torque at the shaft.

The thesis examines the suitability of a CHP project in Bystřice nad Pernštejnem, a small town in Vysočina region, Czech Republic, which is currently supplied by biomass central heating. It reviews situation and availability of renewable energy sources and opportunities for CHP both in the Czech Republic and in Europe, including the context of current global developments. The opening chapters are followed with the renewable energy project for Bystřice nad Pernštejnem itself. The current technology, availability of biomass fuel for a potential future project, and possible development of energy sources in the town are analysed. Three alternative solutions for the CHP plant are suggested, with proposal for the technology and economic analysis, and possible future steps of the town council are discussed.

Master thesis deals with the utilization of waste heat from Nova Mosilana company. Theoretical part of this work is focused on the waste heat description (heat, heat quantity, heat temperature/quality, composition of waste stream) in which a considerable energy potential is hidden. The other parts describe waste heat technology, especially heat pumps, Organic Rankine Cycle (ORC) and system absorption cooling. Some of the technologies described in the theoretical part are used in the design of the more efficient existing waste heat utilization, especifically power and cold production.