For pain research, the orofacial region is unique in a number of ways. The region has complex local anatomy, including substantial sensory innervation from neural pathways, and muscles of facial expression that convey important information concerning pain intensity and associated psychological traits. Although chronic orofacial pain conditions appear prevalent, useful documentation on pain intensity ratings using well established instruments is sparse. In particular, two conditions, atypical facial pain and atypical odontalgia, are poorly understood in aetiology so that definitive treatment modalities are severely limited. The region's local biofluid, saliva, has been used to diagnose various local and systemic disease states, and to quantitate drug concentrations. However, recent studies indicate that saliva also contains some of the same peptides, e.g. bradykinin, that are involved in pain mechanisms. It may be that pharmacological-pharmacokinetic studies of these peptides could shed more information on thesignificance of their presence in saliva. This thesis consists of four major sections. Section 1 comprises of three clinical studies investigating orofacial pain. Section 2 deals with clinical laboratory studies of saliva. Section 3 is concerned with the development of chromatographic methods to assay bradykinin and its pharmacokinetics in saliva. Section 4 uses chromatography for the identification of novel salivary peptides. This thesis, then, presents clinical studies of orofacial pain and pharmacological investigations of saliva as the local biofluid.

Neuropathic pain is defined as "pain initiated or caused by a primary lesion or dysfunction in the nervous system". In contrast to physiological pain that warns of noxious stimuli likely to result in tissue damage, neuropathic pain serves no protective function. Examples of neuropathic pain states include postherpetic neuralgia (shingles) and phantom limb / stump pain. This pain state also exists in the orofacial region, with the possibility of several variants including atypical odontalgia and burning mouth syndrome. There is a paucity of information on the prevalence of neuropathic pain in the orofacial region. One study assessed patients following endodontic treatment and found that approximately 3 to 6percent of patients reported persistent pain. Patients predisposed to the condition atypical odontalgia (phantom tooth pain) include those suffering from recurrent cluster or migraine headaches. Biochemical and neurobiological processes leading to a neuropathic pain state are complex and involve peripheral sensitisation, and neuronal plasticity of the central and peripheral nervous systems. Subsequent associated pathophysiology includes regional muscle spasm, sympathetic hyperfunction, and centralisation of pain. The relevant clinical features of neuropathic pain are: (i) precipitating factors such as trauma or disease (infection), (ii) pain that is frequently described as having burning, paroxysmal, and lancinating or sharp qualities, and (iii) physical examination may indicate hyperalgesia, allodynia and sympathetic hyperfunction. The typical patient complains of persistent, severe pain, yet there are no clearly identifiable clinical or radiographic abnormalities. Often, due to the chronicity of the problem, afflicted patients exhibit significant distress and are poor pain historians, thus complicating the clinician's task of obtaining a detailed and relevant clinical and psychosocial history. An appropriate analgetic blockade test for intraoral sites of neuropathic pain is mucosal application of topical anaesthetics. Other, more specific, tests include placebo controlled lignocaine infusions for assessing neuropathic pain, and placebo controlled phentolamine infusions for sympathetically maintained pain. The treatment and management of neuropathic pain is multidisciplinary. Medication rationalisation utilises first-line antineuropathic drugs including tricyclic antidepressants, and possibly an anticonvulsant. Topical applications of capsaicin to the gingivae and oral mucosa are a simple and effective treatment. Neuropathic pain responds poorly to opioid medication. Psychological assessment is often crucial in developing strategies for pain management. Psychological variables include distress, depression, expectations of treatment, motivation to improve, and background environmental factors. To enable a greater understanding of neuropathic pain, thereby leading to improved treatments, high-performance liquid chromatography-mass spectrometry is one analytical technique that has the potential to contribute to our knowledge base. This technique allows drugs and endogenous substances to be assayed from one sample in a relatively short time. The technique can identify, confirm, and measure the concentrations of multiple analytes from a single sample.

For pain research, the orofacial region is unique in a number of ways. The region has complex local anatomy, including substantial sensory innervation from neural pathways, and muscles of facial expression that convey important information concerning pain intensity and associated psychological traits. Although chronic orofacial pain conditions appear prevalent, useful documentation on pain intensity ratings using well established instruments is sparse. In particular, two conditions, atypical facial pain and atypical odontalgia, are poorly understood in aetiology so that definitive treatment modalities are severely limited. The region's local biofluid, saliva, has been used to diagnose various local and systemic disease states, and to quantitate drug concentrations. However, recent studies indicate that saliva also contains some of the same peptides, e.g. bradykinin, that are involved in pain mechanisms. It may be that pharmacological-pharmacokinetic studies of these peptides could shed more information on thesignificance of their presence in saliva. This thesis consists of four major sections. Section 1 comprises of three clinical studies investigating orofacial pain. Section 2 deals with clinical laboratory studies of saliva. Section 3 is concerned with the development of chromatographic methods to assay bradykinin and its pharmacokinetics in saliva. Section 4 uses chromatography for the identification of novel salivary peptides. This thesis, then, presents clinical studies of orofacial pain and pharmacological investigations of saliva as the local biofluid.

Thesis (Ph. D. in Psychology)--University of Wisconsin--Milwaukee, 1996. / Vita. Photocopy (positive) University Microfilms No. 97-17147. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 61-69).

Thesis (Ph. D. in Psychology)--University of Wisconsin--Milwaukee, 1996. / Vita. Photocopy (positive) University Microfilms No. 97-17147. Includes bibliographical references (leaves 61-69).

Orientador: Alexandre Augusto Zaia / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-04T20:03:13Z (GMT). No. of bitstreams: 1 Menini_MarceloOrlando_M.pdf: 529490 bytes, checksum: 1eb6bdfd00b70b948141086e3729d93a (MD5) Previous issue date: 2005 / Resumo: As dores de origem orofaciais associadas a problemas endodônticos, sejam de origem pulpar ou periapical, são uns dos mais importantes problemas dentro de um serviço de urgência odontológica. Portanto, um diagnóstico correto e tratamento eficaz nesses casos são imprescindíveis para eliminação da dor. O objetivo desse estudo foi avaliar o sucesso do atendimento de urgência endodôntica, realizado por alunos do 4º ano do curso de graduação, em 500 pacientes que procuraram o serviço de plantão de urgência da Faculdade de Odontologia de Piracicaba ¿ UNICAMP, no período de fevereiro de 2003 a maio de 2004. Foram incluídos casos de dor de origem dental, cujo diagnóstico foi confirmado como pulpite irreversível ou necrose pulpar associada à dor periapical, excluindo situação clínica de abscesso. O atendimento clínico de urgência foi realizado conforme preconizado pela disciplina de Endodontia da FOP-UNICAMP e nenhuma medicação foi prescrita aos pacientes. O controle do paciente foi realizado 24 horas após o atendimento, através de telefonema, onde o paciente relatava ausência ou presença de dor pós-operatória. Os resultados mostraram que o atendimento da urgência endodôntica realizado por alunos de graduação no serviço de plantão de urgência da FOP-UNICAMP, apresentou índice de sucesso de 92,6% / Abstract: Orofacial pain from pulpal and periapical disease is one of the most important problem in the emergencies services around the world. Therefore the correct diagnosis and treatment of the tooth are essential to decrease the patient¿s pain. The purpose of this study was to evaluate the success of endodontic emergency appointment in the eliminated of the pain, realized by graduated students in 500 patients, who were attended at the Emergency Service of the Piracicaba Dental School (State University of Campinas), with dental pain, diagnosted as irreversible pulpitis or apical pulp necrosis with periapical pain. The clinical appointment was realized in accord endodontic department of Piracicaba Dental School (State University of Campinas) and no medication was administrated. The follow up of patients was made 24 hours after, the appointment, by phone call, where patients related absence or presence of pain. The results showed that the endodontic emergency treatment, performed at Emergency Service of the Piracicaba Dental School (State University of Campinas) by graduated students had success in 92,6% of cases / Mestrado / Endodontia / Mestre em Clínica Odontológica

O objetivo deste estudo foi avaliar a atividade elétrica dos músculos da mímica de pacientes com dor muscular mastigatória utilizando eletromiografia de superfície. Foram avaliadas 15 mulheres com dor muscular mastigatória (idade média de 33.4, variando de 22-44), grupo de estudo (GMM) e 21 mulheres (idade média de 28.5, variando de 21-45), do grupo controle (GC) Foram realizadas avaliação clínica fonoaudiológica e eletromiográfica (eletromiógrafo ADS 1200, da Lynx Tecnologia Eletrônica Ltda, eletrodos de disco de prata, descartáveis, gel condutor, auto-adesivos com 10mm de diâmetro por HAL Ind. e Com. LTDA) dos músculos: orbicular dos lábios, bucinador, supra-hióideos e masseter em repouso e nos movimentos: protrusão dos lábios, apertamento dos lábios, inflar bochechas, sorriso fechado e sorriso aberto. Houve diferença significativa entre os grupos: os valores da eletromiografia de repouso foram inferiores para o músculo masseter direito (p=0.012) e esquerdo (p=0.019); nos movimentos, os valores eletromiográficos foram superiores no grupo controle durante os movimentos: protrusão dos lábios bucinador esquerdo (p=0.005); suprahióideo esquerdo (p=0.011); apertamento dos lábios bucinador esquerdo (p=0.005); bucinador direito (p=0.007); supra-hióideo esquerdo (p=0.046); supra-hióideo direito (p=0.039); orbicular superior (p=0.042); inflar as bochechas masseter esquerdo (p=0.021); bucinador esquerdo (p=0.007); bucinador direito (p=0.002); orbicular superior (p=0.039); sorriso fechado masseter esquerdo (p=0.004); masseter direito (p=0.019); bucinador esquerdo (p=0.013); supra-hióideo esquerdo (p=0.046) e no sorriso aberto masseter esquerdo (p=0.030). Desta forma, o músculo de maior atividade eletromiográfica foi o orbicular da boca. No repouso, os valores foram inferiores para o masseter e superiores para os demais músculos e nos movimentos, os valores foram superiores em todos os músculos para o grupo de estudo / The objective this study is assess the electric activity of mimic muscles in patients with masticatory muscle pain using surface electromyography (EMG). Was evaluated 15 women with masticatory muscle pain (mean age 33.4, ranging between 22 44 years old), study group (GMM) and 21 women (mean age 28.5, ranging between 21 45 years old), control group (CG). Clinical phonoaudiological and electromyographic assessments were performed (electromyograph ADS 1200 of Lynx Tecnologia Eletrônica Ltda, 10mm disposable pregelled self-adhesive silver disk electrodes of HAL Ind. e Com. LTDA) in the muscles: orbicularis oris, buccinator, suprahyoid and masseter, both at rest and in movement: lip protrusion, lip contraction, cheek inflation, close smile and open smile. There was significant difference between the groups: the values of the electromyography at rest were lower for the right and left masseter muscle (p=0.012 and p=0.019, respectively); in movement, the eletromyographic values were higher in the group control during movement: lip protrusion right buccinator (p=0.005); left suprahyoid (p=0.011); lip contraction left buccinator (p=0.005); right buccinator (p=0.007); left suprahyoid (p=0.046); right suprahyoid (p=0.039); orbicularis oris (p=0.042); cheek inflation left masseter (p=0.021); left buccinator (p=0.007); right buccinator (p=0.002); orbicularis oris (p=0.039); closed smile left masseter (p=0.004); right masseter (p=0.019); left buccinator (p=0.013); left suprahyoid (p=0.046); open smile left masseter (p=0.030). Thus, the orbicularis oris muscle presented the highest EMG activity. At rest, the values were lower for the masseter and higher for the other muscles and, in movement, the values were higher for all the muscles in the study group

published_or_final_version / Dentistry / Doctoral / Doctor of Philosophy

Background Pain and dysfunction in the temporomandibular region (Temporomandibular Disorders, TMD) are common conditions in the general population with an estimated treatment need of 5-15% in the general population. However, in Sweden, traceable performed treatments are significantly lower. The reasons for this indicated under-treatment are not known. To easily detect patients with a potential TMD related condition, three screening questions, 3Q/TMD, have been introduced. The aim with this project was to evaluate the validity and outcome of the 3Q/TMD with the objective to recognize individuals with TMD and potential treatment need in the Public Dental Health service (PDHS). Methods The thesis is based on four study samples; three from the PDHS in the county of Västerbotten, Sweden and one sample from the specialized orofacial pain clinic at the Academic Centre for Dentistry, ACTA, Amsterdam, the Netherlands. The bases for all studies are individuals’ answers to the 3Q/TMD and quantitative approaches were used in the analyses. The prevalence of affirmative answers was evaluated in a large cross-sectional study over the lifespan. The validity of the 3Q/TMD in relation to the Diagnostic Criteria for TMD (DC/TMD) as reference standard was established in two case-control studies. The outcome of the 3Q/TMD on TMD treatment within general practice was evaluated in a cohort study. Results Affirmative answers to the 3Q/TMD increased during adolescence. Women answered affirmatively to the 3Q/TMD significantly more often compared to men for all age groups expect for the first and last parts of a one hundred-year lifespan. The highest prevalence was reported by women during their fertile period in life. In a general population sample, 74% of individuals with an affirmative answer qualified for a DC/TMD pain or dysfunctional diagnosis, as compared to 64% in a specialist patient sample. In the general population sample, for the individual 3Q/TMD questions, as well as combinations of questions, the negative predictive values were high (0.92-0.99). For the specialist sample, when at least one question was answered affirmatively the negative predictive value was high (0.90). The positive predictive value was high (0.89) when all three questions were positive. There was significantly more treatment performed or recommended for 3Q-positives (21.5%) compared to 3Q-negatives (2.2%) (P<0.001). The odds ratio for TMD-related treatment for 3Q-positives versus 3Q-negatives was 12.1 (95% CI: 6.3-23.4). Conclusion The 3Q/TMD is a convenient and valid tool to recognize individuals who would benefit from a further TMD examination within an adult, general population. Within specialized orofacial pain clinics, the questions are useful as guidance for further diagnostics. Although the 3Q/TMD was a factor related to TMD treatment, the majority of individuals with self-reported symptoms of TMD still did not receive traceable assessment or treatment. Factors associated with dentists’ clinical decision-making in relation to TMD warrants further research. The utilization of the 3Q/TMD as a part of a decision tree for the clinician can improve the health care for patients with TMD and is therefore recommended within dentistry. / Bakgrund Smärta och funktionsstörningar i käksystemet (TMD) är vanligt förekommande och de flesta av dessa patienter kan behandlas framgångsrikt i tandvården. Tidigare studier har visat att det kan finnas en skillnad mellan det skattade vårdbehovet och utförd behandling för patienter med TMD i tandvården. Anledningen till detta är inte känt och har identifierats som en kunskapslucka av Socialstyrelsen. Den vanligaste orsaken till akut smärta i ansiktsregion är tandvärk medan kronisk smärta oftast är relaterat till smärttillstånd i muskulatur och käkled (TMD). Att leva med smärta har en negativ påverkan på livskvaliteten oavsett om smärtan är orsakad av tandvärk eller TMD. Kvinnor uppger symtom på TMD ungefär två gånger så ofta som män och förekomsten är som högst bland kvinnor i arbetsför ålder. Smärtan är ofta relaterad till stress och långvarig smärta ökar risken för såväl spridning till andra lokalisationer som till sömnbesvär och nedstämdhet. Tidig intervention har visat sig betydelsefull för att minska risken för negativa konsekvenser relaterat till kroniska besvär. Sammantaget visar detta på vikten av ett system inom tandvården för att tidigt identifiera patienter som är i möjligt behov av fördjupad utredning och eventuellt behandling. Därför har tre screeningfrågor för TMD, 3Q/TMD införts inom tandvården. I Folktandvården i Västerbotten infördes frågorna i maj 2010 som en obligatorisk del av den digitala hälsodeklarationen för samtliga patienter. Syfte Syftet med avhandlingen har varit att synliggöra patienter med smärta och käkfunktionsstörning i befolkningen och att därmed förbättra omhändertagandet av patienter med TMD inom Folktandvården. De specifika målen var att: i) beskriva förekomst av frekvent smärta och dysfunktion i ansikte, käke och käkled för män och kvinnor för olika åldersgrupper baserat på tre screeningfrågor ii) bedöma de tre screeningfrågornas validitet i förhållande till en diagnos enligt Diagnostic Criteria for TMD, DC/TMD i två olika populationer iii) utvärdera utfallet av 3Q/TMD på klinisk beslutsprocess inom Folktandvården i Västerbotten, Sverige. Studiepopulation och metod Avhandlingen baserades på fyra olika urval av patientpopulationer, tre från Folktandvården i Västerbotten, Sverige och en från Specialistkliniken från Academish Centrum Tandheelkunde Amsterdam, ACTA, Amsterdam, Nederländerna. Underlaget för samtliga studier var baserade på individuella svar på de tre screeningfrågorna. Förekomsten av de som svarat ja på någon av frågorna utvärderades i en tvärsnittsstudie bland nästan 140 000 individer och över ett helt livsspann. Validiteten för 3Q/TMD i relation till en diagnos enligt DC/TMD utvärderades i två fall-kontroll studier. Först undersöktes en studiepopulation från Folktandvården i Västerbotten, Sverige för att representera allmän befolkningen. Därefter utvärderades screeningfrågorna bland de patienter som remitterats till en specialistklinik i Amsterdam, Nederländerna på grund av TMD relaterade besvär. Dessa representerar remitterade patienter. Utfallet av 3Q/TMD på TMD behandling i Folktandvården utvärderades i en prospektiv kohort studie. Resultat Förekomsten av de som svarat ja på någon av frågorna ökar under tonåren. Kvinnor svarar ja på någon av frågorna signifikant oftare än män i alla åldrar, utom i början och slutet av livsspannet. Den högsta prevalensen noterades för kvinnor i fertil ålder. I allmänbefolkningen kvalificerar 74% av de som svarat ja på någon av frågorna även för en DC/TMD diagnos. Motsvarande siffra för urvalet från specialistkliniken var 64%. I urvalet från allmänbefolkningen var det negativa prediktiva värdet högt för såväl alla enskilda frågor som för kombinationer av frågor (0.92-0.99). Bland de remitterade patienterna, var negativt prediktivt värde högt när individen svarade ja på minst en fråga (0.90). Positivt prediktivt värde var högt när individen svarat ja på alla tre frågorna (0.89). Behandling hade utförts eller rekommenderats signifikant oftare till de som svarat ja på någon av frågorna (21.5%) jämfört med de som svarat nej på alla tre frågor (2.2%) (P<0.001). Odds ratio för TMD behandling för 3Q-positiva jämfört med 3Q-negativa var 12.1 (95% CI:6.3-23.4). Slutsats Sammanfattningsvis visar resultaten att en betydande andel av befolkningen, framför allt kvinnor i arbetsför ålder, har smärta och/eller funktionsstörningar i käksystemet. Frågorna är lämpliga för att screena patienter i behov av en fördjupad utredning. De som svarat nej kommer med stor sannolikhet inte att kvalificera för en diagnos enligt DC/TMD. Bland remitterade patienter kommer majoriteten att svara ja på någon av frågorna. I allmäntandvården får två tredjedelar av det som svarat ja på minst en fråga, inte ett synliggjort omhändertagande baserat på vad som är angivet i deras tandvårdsjournal. Detta kan tyda på brister bland allmäntandläkare och tandhygienister i kliniskt beslutsfattande för patienter med TMD.

Disfunção temporomandibular (DTM) é um termo coletivo que engloba um largo espectro de alterações articulares e musculares no sistema estomatognático. Estas disfunções são caracterizadas por dor, ruídos articulares e funções irregulares da mandíbula, e representam a principal causa de dor não dentária da região orofacial. A prevalência de indivíduos com necessidade de tratamento está entre 5 e 12%, calcula-se que no Brasil 8,5 milhões de brasileiros precisariam ter algum tipo de intervenção. Estudos epidemiológicos sobre DTM têm se apresentado deficientes quanto à padronização de índices e classificações. Conscientes disso realizou-se um levantamento de prontuários dos pacientes atendidos no Serviço de Oclusão, Disfunção Temporomandibular e Dor Orofacial, do projeto DAPE da Faculdade de Odontologia de Ribeirão Preto USP, que realiza atendimento na área de DTM para população encaminhada pelo Sistema Único de Saúde (SUS), entre os anos de 2010 e 2011. Avaliou-se 117 prontuários de indivíduos, que foram submetidos à classificação do Índice Anamétido de Helkimo, divididos de acordo com a severidade de sinais e sintomas, em dois grupos : AiI (leves a moderados) e AiII (severos). Posteriormente os grupos foram subdivididos com relação ao gênero e faixa etária. Os dados foram coletados por meio de entrevista, onde os indivíduos foram questionados quanto à presença de hábitos parafuncionais, alterações otológicas, oftalmológicas, sistêmicas e comportamentais. Os dados foram tabulados em planilha de Excel, e submetidos à Análise Estatística, utilizando o Teste de Kruskal-Wallis e Teste de Miller, ambos com significância de 0,05. A prevalência de cada alteração também foi avaliada. Os resultados evidenciaram que indivíduos com DTM apresentaram altas prevalências nas alterações locais e sistêmicas pesquisadas. / Temporomandibular (DTM) is a collective term that encompasses a broad spectrum of clinical problems and deformations in orofacial area. These disorders are characterized by pain, joint noises and irregular functions of the jaw, and represent the leading cause of orofacial pain not dental. The prevalence of individuals in need of treatment is between 5% and 12%, it is estimated that in Brazil 8.5 million Brazilians would have some kind of intervention. Epidemiological studies on TMD have presented handicapped on the standardization of indexes and ratings. Aware of this was done a survey of medical records of patients seen in Orofacial Pain service of special Patients clinic FORP-USP, who performs service in the area of TMD for population forwarded by the Central regulating (SUS), the city of Ribeirão Preto, between the years 2010 and 2011. Assessed-if medical records of individuals, these 117 were subjected to the classification of the Anamétido Index of Helkimo, and divided according to the severity of symptoms and signs, in two groups AiI (mild to moderate) and AiII (severe). Later groups were sub-divided in relation to gender and age group. The data were collected through interview, where individuals were questioned as to the presence of parafunctional habits, changes otologicals, ophthalmologicals, systemic and behavioral. The data were tabulated into Excel spreadsheet, and subjected to statistical analysis, using the Kruskal-Wallis test, and Miller\'s Test, both with significance of 0.05. The prevalence of each change was also evaluated. The results showed that TMD patients showed a high prevalence in the surveyed local and systemic changes.