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Physiology of Escherichia coli in batch and fed-batch cultures with special emphasis on amino acid and glucose metabolismHan, Ling January 2002 (has links)
<p>The objective of this work is to better understand themetabolism and physiology of<i>Escherichiacoli</i>(W3110) in defined medium cultures with thelong-term goal of improving cell yield and recombinant proteinproductivity.</p><p>The order of amino acid utilization in<i>E. coli</i>batch cultures was investigated in a medium with16 amino acids and glucose. Ser, Pro, Asp, Gly, Thr, Glu andAla were rapidly consumed and depleted at the end of theexponential phase, while His, Arg, Val, Met, Ile, Leu, Phe, Lysand Tyr were consumed slowly during the following linear growthphase. The uptake order correlated to the maximum specificconsumption rate. Of the rapidly consumed amino acids onlyglyine and threonine improved growth when added individually.Serine was the first amino acid to be consumed, but inhibitedglucose uptake initially, which presumably is related to thefunction of PTS. Valine inhibited cell growth could be releasedby isoleucine. The critical medium concentration of valinetoxicity was 1.5 - 3 µmol L<sup>-1</sup>. Valine uptake was associated with exchange ofisoleucine out of the cells.</p><p>Glycine significantly increased the cell yield,<i>Y</i><sub>x/s,</sub>and growth rate of<i>E. coli</i>in batch cultures in a glucose-mineral medium.Maximum effect occurred at pH 6.8, at 6 - 12 mmol L<sup>-1</sup>glycine, and below 1.15 g dw L<sup>-1</sup>.<sup>13</sup>C NMR technique was employed to identify [1-<sup>13</sup>C], [2-<sup>13</sup>C]and [1,2-<sup>13</sup>C]acetate in the cultures supplied with [2-<sup>13</sup>C]glycine. The NMR data revealed that littledegradation of added glycine occurred, and that serine/glycinebiosynthesis was repressed below 1.15 g dw L<sup>-1</sup>, implicating that glycine was a source ofglycine, serine, one-carbon units, and threonine. Above 1.15 gdw L<sup>-1</sup>, 53% of the consumed glycine carbon was excretedas acetate. Degradation of glycine was associated with anincreased uptake rate, cleavage by GCV, and degradation of bothglycine- and glucose-derived serine to pyruvate. This switch inmetabolism appears to be regulated by quorum sensing.</p><p>A cell density-dependent metabolic switch occurred also inthe central metabolism. A 2 - 3 fold decrease in mostglycolytic and TCA cycle metabolites, but an increase inacetyl-CoA, occurred after the switch. The acetate productionrate decreased throughout the culture with a temporary increaseat the switch point, but the intracellular acetate poolremained relatively constant.</p><p>Two mixtures of amino acids were fed together with glucosein fed-batch cultures of<i>E. coli</i>W3110 pRIT44T2, expressing the recombinantprotein ZZT2. One mixture contained 20 amino acids and theother 5 so-called 'protein amino acids': Ala, Arg, Met, His andPhe. Although the amino aids increased the cell yield anddecreased the proteolysis rate in both cases, ZZT2 productionwas decreased. A decrease of ZZT2 synthesis rate is consideredto be the reason. Further studies of the 5 amino acidsindicated that a few amino acids disturb metabolism.</p><p>Carbon mass balances were calculated in glucose limitedfed-batch cultures of<i>E. coli</i>. In the end, the carbon recovery was ~90% basedon biomass, CO<sub>2</sub>and acetate, but ~100% if the all carbon in themedium was included. Outer membrane (OM) constituents,lipopolysaccharide, phospholipids, and carbohydratescontributed to 63% of the extracellular carbon. Little celllysis occurred and the unidentified (~30%) carbon was assumedto constitute complex carbohydrates. A novel cultivationtechnique Temperature-Limited Fed-Batch (TLFB) is developed toprevent OM shedding in high-cell density cultures.</p><p><b>Keywords</b>: Escherichia coli, amino acids, glycine, quorumsensing, metabolic switch, metabolite pools, carbon balance,outer membrane, lipopolysaccharide, batch culture, fed-batchculture</p>
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Function and regulation of the delta subunit of PDE6 /Cook, Terry Ann, January 2001 (has links)
Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves 113-137).
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Using Live Cell Imaging to Probe Biogenesis of the Gram-Negative Cell EnvelopeYao, Zhizhong January 2012 (has links)
In Gram-negative bacteria, the three-layered cell envelope, including the cell wall, outer and inner membranes, is essential for cell survival in the changing, and often hostile environments. Conserved in all prokaryotes, the cell wall is incredibly thin, yet it functions to prevent osmotic lysis in diluted conditions. Based on observations obtained by genetic and chemical perturbations, time-lapse live cell imaging, quantitative imaging and statistical analysis, Part I of this dissertation explores the molecular and physical events leading to cell lysis induced by division-specific beta-lactams. We found that such lysis requires the complete assembly of all essential components of the cell division apparatus and the subsequent recruitment of hydrolytic amidases. We propose that division-specific beta-lactams lyze cells by inhibiting FtsI (PBP3) without perturbing the normal assembly of the cell division machinery and the consequent activation of cell wall hydrolases. On the other hand, we demonstrated that cell lysis by beta-lactams proceeds through four physical phases: elongation, bulge formation, bulge stagnation and lysis. Bulge formation dynamics is determined by the specific perturbation of the cell wall and outer membrane plays an independent role in stabilizing the bulge once it is formed. The stabilized bulge delays lysis, and allows escape and recovery upon drug removal. Asymmetrical in structure and unique to Gram-negative bacteria, outer membrane prevents the passage of many hydrophobic, toxic compounds. Together with inner membrane and the cell wall, three layers of the Gram-negative cell envelope must be well coordinated throughout the cell cycle to allow elongation and division. Part II of this dissertation explores the essentiality of the LPS layer, the outer leaflet of the outer membrane. Using a conditional mutant severely defective in LPS transport, we found that mutations in the initiation phase of fatty acid synthesis suppress cells defective in LPS transport. The suppressor cells are remarkably small with a 70% reduction in cell volume and a 50 % reduction in growth rate. They are also blind to nutrient excess with respect to cell size control. We propose a model where fatty acid synthesis regulates cell size in response to nutrient availability, thereby influencing growth rate. / Chemistry and Chemical Biology
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Changing pictures of social science theory and practice : a Wittgensteinian approach to human mind and experienceJones, Donald Earl, 1957- 31 January 2011 (has links)
This dissertation argues that there is a set of assumptions--or a picture, in Wittgenstein's language--that influences our thinking about who we are as human beings and our relationships to the rest of the world. These assumptions have their origins in Cartesianism and function as unrecognized, unacknowledged foundations on which all of the rest of our thinking and acting takes place. My argument is that these assumptions are deeply problematic and that we need to both examine the impact of those assumptions and beginning building alternative perspectives. I draw primarily from scholars who build upon a Wittgensteinian perspective that draws upon the Philosophical investigations, On certainty, and other volumes of Wittgenstein's work that have been published since the Philosophical Investigations. These scholars include Taylor (2007), Williams (2002), Mulhall (2007), Canfield (2004, 2007), Moyal-Sharrock (2004), Travis (2006, 2007), Schatzki (1996, 2001), and Stroll (2002, 2004). Of particular interest to me is the inner-outer distinction--or in Taylor's terms, dualist sorting--of Cartesian dualism, whereby all mental processes are contained within individual human minds that are separate and distinct from the rest of the reality. Taylor, Williams, Schatzki, and other Wittgensteinian scholars argue that this assumption continues to be relatively unacknowledged and unchallenged despite a long history of philosophical challenges to the Cartesian perspective. These scholars argue that the inner-outer distinction is deeply mistaken and yet continues to have an impact on contemporary life that is both pervasive and negative. A key part of my approach builds on Taylor's (2007) argument about the connection between ontology and epistemology within the Cartesian picture. Taylor argues that we get to a new picture only by carefully investigating the influences of the Cartesian picture and then building a new perspective out of alternatives to each piece of the Cartesian picture. Canfield (2004) argues similarly, referring to this as a bottom-up approach. In this work, I look at both theoretical and applied issues within the social sciences. I investigate how a few concrete practices play out within specific contexts when considered from an alternative perspective that takes unmediated knowledge and embodied practices (Taylor, 2007), a social conception of mind (Williams, 2002), and a relational ontology (Slife, 2004) as foundational. And finally, I present specific examples drawn from the applied practices of the social sciences with a focus on the delivery of psychological services (including psychology, psychotherapy, and counseling) and the teaching of communication (including writing, speaking, and interpersonal communication). The purpose of these examples is to bring out some of the contradictions and problems that occur because of the unacknowledged assumptions of the Cartesian picture and to show the kinds of solutions that an alternative perspective can provide. My goal is to provide concrete suggestions for thinking and acting within the context of particular practices using psychotherapy and teaching as the primary sources for examples. / text
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Expansion of perturbation theory applied to shim rotation automation of the Advanced Test ReactorPeterson, Joshua Loren 19 October 2011 (has links)
In 2007, the Department of Energy (DOE) declared the Advanced Test Reactor (ATR) a National Scientific User Facility (NSUF). This declaration expanded the focus of the ATR to include diversified classes of academic and industrial experiments. An essential part of the new suite of more accurate and flexible codes being deployed to support the NSUF is their ability to predict reactor behavior at startup, particularly the position of the outer shim control cylinders (OSCC). The current method used for calculating the OSCC positions during a cycle startup utilizes a heuristic trial and error approach that is impractical with the computationally intensive reactor physics tools, such as NEWT. It is therefore desirable that shim rotation prediction for startup be automated. Shim rotation prediction with perturbation theory was chosen to be investigated as one method for use with startup calculation automation. A modified form of first order perturbation theory, called phase space interpolated perturbation theory, was developed to more accurately model shim rotation prediction. Shim rotation prediction is just one application for this new modified form of perturbation theory. Phase space interpolated perturbation theory can be used on any application where the range of change to the system is known a priori, but the magnitude of change is not known. A cubic regression method was also developed to automate shim rotation prediction by using only forward solutions to the transport equation. / text
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Immobilizing Mutation in an Unconventional Myosin15a Affects not only the Structure of Mechanosensory Stereocilia in the Inner Ear Hair Cells but also their Ionic ConductancesSyam, Diana 01 January 2014 (has links)
In the inner and outer hair cells (OHCs) of the inner ear, an unconventional myosin 15a localizes at the tips of mechanosensory stereocilia and plays an important role in forming and maintaining their normal structure. A missense mutation makes the motor domain of myosin 15a dysfunctional and is responsible for the congenital deafness DFNB3 in humans and deafness and vestibular defects in Shaker-2 (Sh2) mouse model. All hair cells of homozygous Shaker-2 mice (Myo15sh2/sh2) have abnormally short stereocilia, but, only stereocilia of Myo15sh2/sh2OHCs start to degenerate after the first few days of postnatal development and lose filamentous tip links between stereocilia that are crucial for mechanotransduction. The exact mechanisms of this degeneration are unknown even though they may underlie DFNB3 deafness in humans. We hypothesize that structural abnormalities in Myo15sh2/sh2 OHCs may alter the mechanical forces applied to the mechano-electrical transduction (MET) channels resulting in abnormal ionic homeostasis, which may lead to eventual degeneration of Myo15sh2/sh2 OHCs. Therefore, we investigated the ionic conductances and integrity of mechanotransduction apparatus in Myo15sh2/sh2 OHCs. Surprisingly, we found that myosin 15a-deficiency is associated not only with structural abnormalities of OHC stereocilia but also with alterations of voltage-gated ion conductances.
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Global retrievals of upper-tropospheric phosphine from the Cassini/CIRS Jupiter encounterParrish, Paul David January 2004 (has links)
On December 30th 2000, the Cassini-Huygens spacecraft reached the perijove milestone in its continuing journey to the Saturnian system. During an extended six-month encounter, the Composite Infrared Spectrometer (CIRS) returned spectra of the Jovian atmosphere, rings and satellites from 10 to 1400 cm^-1 (1000 to 7 µm) at a programmable spectral resolution of 0.5 to 15 cm^-1. The improved spectral resolution of CIRS over previous infrared instrument-missions to Jupiter, the extended spectral range and higher signal-to-noise performance provide significant advantages over previous data-sets. Both optimal-estimation retrieval and radiance-differencing are used to investigate the global variation of upper-tropospheric temperature, ammonia, phosphine and cloud opacity between ± 60˚ latitude. The analysis methods are shown to successfully reproduce Jovian conditions with results consistent with previous investigations. The composition results in particular are well characterised and suggest an important role played by mixing and transport within the upper-troposphere. Interpretation and validation of the retrieved results is conducted via the construction of a simple dynamic model incorporating transport, diffusion and (photo)chemistry.
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Implementing international standards for "continuing supervision"Spencer, Ronald L. January 2008 (has links)
The Outer Space Treaty established the obligation to provide continuing supervision of its national space activities by the appropriate state. The implementation of this obligation remains a matter of state discretion. Since this Treaty came into force the world has evolved to become reliant on space based utilities to enable the global economy and state governance. Today, space faring states are increasingly dependent upon the supervision practices of other states to assure its space interests as the attribution of state responsibility becomes more difficult to ascribe. / Therefore, the absence of binding supervision standards may become an impediment to future space applications due to three identified trends. First, the trend towards space commercialization requires active state supervision. Second, the rise in environmental hazards requires minimal safety standards to decrease the harmful effects on space applications. Third, space security requires identification of intentional acts and prudent measures to safeguard vital space applications.
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Molecular characterisation of Shigella flexneri outer membrane protease IcsP.Tran, Elizabeth Ngoc Hoa. January 2008 (has links)
Shigella is a genus of Gram-negative bacteria responsible for bacillary dysentery in humans. Shigella flexneri type 2a in particular is responsible for the majority of incidents in developing countries. The S. flexneri protease IcsP, is a member of the Omptin family of outer membrane (OM) proteases which cleaves IcsA, a polarly localised OM protein required for Shigella virulence. Mutations in icsP have been shown to effect the observed distribution of IcsA, however the significance of IcsP in Shigella virulence is incompletely understood. In this study, aspects of IcsP biology were investigated. S. flexneri 2457T and M90T icsP mutants were constructed to investigate the role of IcsP in Shigella intercellular spread, and it was found that icsP in both S. flexneri backgrounds did not appear to be essential for cell-tocell spread in human cervical cancer HeLa cells, but enhanced cell-to-cell spread in monkey kidney CV-1 cells (as determined by plaque assays). Complementation with icsP returned the mutant phenotype to wild-type. The results suggest IcsP does play a role in Shigella intercellular spread. The 2457T icsP mutant was subsequently complemented with an altered icsP gene encoding a haemagglutinin epitope tagged IcsP (IcsPHA) to determine the distribution of IcsP on the cell surface. In both S. flexneri and E. coli K-12 possessing smooth and rough lipopolysaccharide (LPS), the distribution of IcsPHA was found to be punctate across the cell surface. Deconvolution analysis revealed that IcsP distribution was punctate and banded in both LPS backgrounds. A smooth LPS E. coli K-12 yfdI mutant strain expressing IcsPHA was also constructed, and experiments involving treatment of this strain with bacteriophage Sf6 tail spike protein suggested that LPS O antigen chains masked IcsP in smooth LPS strains. During these studies, double-labelling of IcsPHA and LPS in a S. flexneri 5a M90T strain revealed a helical distribution of LPS in this strain. Overall, the results suggest IcsP has a punctate, banded distribution across the cell surface. The effect of virK and rmlD mutations on IcsP was then investigated by constructing a virK, rmlD and virK/rmlD double mutant in S. flexneri 2457T. Western immunoblotting showed no change in IcsP expression levels in either the virK, rmlD or virK/rmlD mutants compared to wild-type. Surprisingly, the virK mutant showed no change in IcsA expression levels by Western immunoblotting and plaque assays (using HeLa and CV-1 cells) suggested that virK was not essential for Shigella intercellular spread (contradicting the published data on this gene). No effect was also observed on IcsP expression level or on IcsP’s ability to cleave IcsA into culture supernatants. Finally alternative substrates for the protease activity of IcsP were investigated against known Omptin substrates (plasminogen, α2-antiplasmin, complement, protamine and colicins). However, IcsP appeared to have no effect on these substrates as determined by proteolytic cleavage assays and antimicrobial assay. Interestingly, Plg cleavage by rough LPS S. flexneri, and α2AP cleavage by both smooth and rough LPS S. flexneri, was observed. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1339487 / Thesis (Ph.D.) - University of Adelaide, School of Molecular and Biomedical Science, 2008
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Outer membrane protein immunity to Pasteurella pneumotropica and the interaction of allergySee, Sarah Bihui January 2010 (has links)
[Truncated abstract] Infectious and allergic diseases of the respiratory tract are major contributors to global mortality, morbidity and economic burden. Bacterial infections such as pneumonia and otitis media are important diseases, especially in children, while allergic diseases such as asthma and allergic rhinitis afflict up to 30% of the world's population. A confounding aspect of respiratory disease is the evidence of a complex relationship between respiratory allergy and respiratory infection, with infection suggested to both promote and prevent the pathogenesis of allergic disease. Additionally, allergy is a risk factor for bacterial infection such as otitis media, pneumonia and sinusitis, while respiratory infection can exacerbate allergic symptoms. Given the burden of bacterial respiratory disease and respiratory allergy, the development of preventative treatments for these diseases is needed and will benefit from clearer knowledge of the underlying immune mechanisms. This thesis aimed to to extend current knowledge by using Pasteurella pneumotropica, a similar bacteria to the human pathogen nontypeable Haemophilus influenzae (NTHi), to study respiratory infection and protective anti-outer membrane protein (OMP) immunity as well as the interaction of respiratory infection and allergic inflammation. Homologues of the important NTHi vaccine candidates P4, P6, P26 and D15 were found to be encoded by P. pneumotropica and a high level of amino acid sequence identity was noted between the different P. pneumotropica strains, as well as between other Pasteurellaceae members. ... In contrast, anti-P6his serum antibodies transferred to naïve mice did not confer protection. These results suggested that T-cellmediated mechanisms were involved in P6his-mediated protection, and showed that the P. pneumotropcia model was useful for elucidating protective mechansims. The interaction of P. pneumotropica infection and papain-induced allergy was studied to investigate immune mechanisms underlying respiratory infection and allergy. Mice with ongoing allergic inflammation were intranasally challenged with bacteria and exhibited reduced pulmonary bacterial numbers, prolonged eosinophilia in the lungs and the induction of Th2 cytokines in the BALF, compared to nonallergic, infected mice. This suggested a protective role for allergic inflammation in this model. The effect of papaininduced inflammation on mice colonised by P. pneumotropica was also examined and allergic inflammation appeared to worsen infection in colonised mice. This suggested that allergic inflammation may also have a role in promoting infection in this model. In conclusion, this thesis explored mechanisms involved in vaccine-mediated immunity and the interaction of respiratory infection and allergy using a P. pneumotropica infection in its natural host. It was shown that intranasally administered recombinant P6 and P4 protected mice from lung infection, which justifies the inclusion of these OMPs as NTHi vaccine candidates. Additionally, it was demonstrated that the interaction of allergy and respiratory infection modulated immune responses. Overall, these results emphasize that a clearer understanding of the complex mechanisms underlying these interactions is required, and may be aided by the development of suitable animal models.
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