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Pathophysiological aspects of severe falciparum malaria in Thailand and GhanaAngus, Brian John January 2001 (has links)
No description available.
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Trypanosmoma brucei : Studies on the uptake and effects of daunorubicin and daunorubicin carrier preparationsGolightly, L. January 1986 (has links)
No description available.
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Development of a vaccine against the human hookworm, Necator americanusGirod, Nadine January 2001 (has links)
No description available.
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Parasite-induced changes in host behaviourEdwards, J. C. January 1987 (has links)
No description available.
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The behavioural control of helminth infection by sheepCooper, Jane January 1996 (has links)
Parasites have detrimental effects on an animal's fitness and could play a significant role in shaping the adaptive behaviour of animals. Natural selection may favour those behaviours utilised by animals that minimise the risk and intensity of infections. Animals use a range of behavioural strategies associated with grooming, social, mating, migratory and foraging behaviours to minimise their parasite burdens. Herbivores have two means available for reducing the effects of parasites while foraging. They may avoid parasites or consume plants with anti-parasitic properties. Sheep (Ovis aries) were shown to avoid foraging in areas of the sward contaminated with O. circumcincta infective larvae but could only do so when larvae were associated with faeces. The ability of sheep to avoid contaminated patches of the sward increased as the size of contaminated sward patches increased. This avoidance behaviour resulted in a reduction in the numbers of parasites ingested. Animals infected with O. circumcincta were more selective in their grazing behaviour than uninfected animals with respect to faeces avoidance. In order to determine which plants possess anti-parasitic properties Lotus pedunculatus, Pinus sylvestris, Tanacetum vulgare and Artemisia absinthium extracts were screened against Trichostrongylus colubriformis infections in the Mongolian gerbil (Meriones unguiculatus). Only A. absinthium merited further investigation as it appeared to suppress worm burdens when administered in a high single dose. A. absinthium extracts were subsequently administered to sheep infected with T. colubriformis.
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Epidemiology of the caecal threadworm Trichostrongylus tenuis in red grouse (Lagopus lagopus scoticus Lath.)Shaw, Jennifer Lyn January 1988 (has links)
The prevalence of Trichostrongylus tenuis in red grouse was high and the distribution of parasites in their hosts highly aggregated. The prevalence and intensity of threadworms were greater in old birds than in young ones. There was no relationship between the body condition of individual grouse and their burden of adult worms. On the moor, T. tenuis eggs did not develop to third-stage infective larvae during the winter. In the summer, when development did occur, yields of third-stage larvae were dependent on temperature. Third-stage larvae moved laterally through the heather but were relatively short-lived and very few survived over winter. The proportion of larvae, ingested by captive grouse, which developed to adult worms varied with season and between individuals. Red grouse acquired little or no effective immunity to reinfection after challenge with infective larvae. Consequently, caecal threadworms produced a chronic infection in grouse: mature worms survived for over two years with very little mortality. Parasite fecundity decreased as the worms aged but not with intensity of infection. Third-stage larvae ingested by wild red grouse in the autumn were found to go into arrested development over the winter, resuming their development in the spring. Captive grouse with parasite burdens were later in starting to lay in spring and laid fewer eggs at a slower rate than uninfected hens. However developing larvae had greater effects than adult worms on egg production. Effects of developing larvae included inappetence and a reduced gain in body weight prior to laying. Consequently, overwintering larvae developing simultaneously in spring may reduce the breeding success of wild red grouse. This observation may also explain why outbreaks of trichostrongylosis are usually found in spring and summer. The transmission of T. tenuis is presumably influenced mainly by the density of infective larvae, which in turn was affected by weather, particularly temperature. A major regulatory constraint on this parasite is host mortality. Mortality, however, is unlikely to be linearly related to parasite intensity because developing larvae were more pathogenic than adult worms. Furthermore, there seemed to be no important intensity-dependent processes acting to regulate T. tenuis in red grouse populations. On the moor, T. tenuis eggs did not develop to third-stage infective larvae during the winter. In the summer, when development did occur, yields of third-stage larvae were dependent on temperature. Third-stage larvae moved laterally through the heather but were relatively short-lived and very few survived over winter. The proportion of larvae, ingested by captive grouse, which developed to adult worms varied with season and between individuals. Red grouse acquired little or no effective immunity to reinfection after challenge with infective larvae. Consequently, caecal threadworms produced a chronic infection in grouse: mature worms survived for over two years with very little mortality. Parasite fecundity decreased as the worms aged but not with intensity of infection. Third-stage larvae ingested by wild red grouse in the autumn were found to go into arrested development over the winter, resuming their development in the spring. Captive grouse with parasite burdens were later in starting to lay in spring and laid fewer eggs at a slower rate than uninfected hens. However developing larvae had greater effects than adult worms on egg production. Effects of developing larvae included inappetence and a reduced gain in body weight prior to laying. Consequently, overwintering larvae developing simultaneously in spring may reduce the breeding success of wild red grouse. This observation may also explain why outbreaks of trichostrongylosis are usually found in spring and summer. The transmission of T. tenuis is presumably influenced mainly by the density of infective larvae, which in turn was affected.
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Characterisation of Community-Derived Hymenolepis Infections in AustraliaMarion.Macnish@abcrc.org.au, Marion Macnish January 2001 (has links)
Hymenolepis nana is a ubiquitous parasite, found throughout many developing and developed
countries. Globally, the prevalence of H. nana is alarmingly high, with estimates of up to 75
million people infected. In Australia, the rates of infection have increased substantially in the
last decade, from less than 20% in the early 1990s to 55 - 60% in these same communities
today. Our knowledge of the epidemiology of infection of H. nana is hampered by the
confusion surrounding the host specificity and taxonomy of this parasite. The suggestion of the
existence of two separate species, Hymenolepis nana von Siebold 1852 and Hymenolepis
fraterna Stiles 1906, was first proposed at the beginning of the 20th century. Despite ongoing
discussions in the subsequent years it remained unclear, some 90 years later, whether there were
two distinct species, that are highly host specific, or whether they were simply the same species
present in both rodent and human hosts. The ongoing controversy surrounding the taxonomy of
H. nana has not yet been resolved and remains a point of difference between the taxonomic and
medical literature.
The epidemiology of infection with H. nana in Australian communities is not well understood
as the species present in these communities has never been identified with certainty. It is not
clear which form of transmission commonly occurs in Australia, whether the H. nana
strain/species present in the north-west of Western Australia is present in human and rodent
hosts, or whether humans harbour their own strain/sub-species of Hymenolepis. Furthermore,
it is not known whether mice are a potential zoonotic source for transmission of Hymenolepis to
human hosts. In this study, 51 human isolates of H. nana were inoculated into highly
susceptible laboratory rodent species. However, these failed to develop into adult worms in all
instances, including when rodent species were chemically and genetically immunosuppressed.In addition, 24 of these human isolates were also cross-tested in the flour beetle intermediate
host, Tribolium confusum. Of these, only one isolate developed to the cysticercoid stage in
beetles, yet when inoculated into laboratory rodents, the cysticercoids also failed to develop into
adult stage.
Since isolates of H. nana infecting humans and rodents are morphologically indistinguishable,
the only way they can be reliably identified is by comparing the parasite in each host using
molecular criteria. In the current study, three regions of ribosomal DNA, the small subunit
(18S), the first internal transcribed spacer (ITS1) and the intergenic spacer (IGS) were chosen
for genetic characterisation of Hymenolepis spp. from rodent and human hosts from a broad
geographic range. In addition, a mitochondrial gene, the cytochrome c oxidase subunit 1 (C01)
gene and a non-ribosomal nuclear gene, paramyosin, were characterised in a number of
Hymenolepis isolates from different hosts.
A small PCR fragment of 369 bp, plus a larger fragment of 1223 bp, were sequenced from the
18S gene of reference isolates of H. nana and the rat tapeworm H. diminuta. Minimal sequence
variation was found in the two regions of the 18S between these two morphologically distinct,
phylogenetically recognised species, H. nana and H. diminuta, and this indicated that the 18S
gene was too conserved for further genetic characterisation of isolates of H. nana from different
hosts.
A large number of human isolates of H. nana (104) were characterised at the ITS1 using PCRrestriction
length fragment polymorphism (PCR-RFLP). The profiles obtained were highly
variable and often exceeded the original size of the uncut fragment. This was highly suggestive
of the existence of ribosomal spacers that, whilst identical in length, were highly variable in
sequence. To overcome the problems of the variable PCR-RFLP profiles, further
characterisation of the ITS1, by cloning and sequencing 23 isolates of H. nana, was conducted
and this confirmed the existence of spacers which, although similar in length (approximately
646 bp), differed in their primary sequences. The sequence differences led to the separation of
the isolates into two clusters when analysed phylogenetically. This sequence variation was not,
however, related to the host of origin of the isolate, thus was not a marker of genetic distinction
between H. nana from rodents and humans. Indeed, the levels of variability were often higher
within an individual isolate than between isolates, regardless of whether they were collected
from human or mice hosts, which was problematic for phylogenetic analysis. In addition, mixed
parasite infections of H. nana and the rodent tapeworm H. microstoma were identified in four
humans in this study, which was unexpected and surprising, as there have been no previous
reports in the literature documenting humans as definitive hosts for this parasite. Further studies
are required, however, to determine if the detection of H. microstoma in humans reflects a
genuine, patent infection or an atypical, accidental occurrence.
Sequencing of the mitochondrial cytochrome c oxidase 1 gene (C01) in a number of isolates of
Hymenolepis nana from rodents and humans identified a phylogenetically supported genetic
divergence of approximately 5% between some mouse isolates compared to isolates of H. nana
from humans. This provided evidence that the mitochondrial C01 gene was useful for
identifying genetic divergences in H. nana that were not resolvable using nuclear loci. Despite
a morphological identity between isolates of H. nana from rodent and human hosts, the genetic
divergence observed between isolates at the mitochondrial locus was highly suggestive that
H. nana is a species complex, or cryptic species (= morphologically identical yet genetically
distinct). In addition, whilst not supported by high bootstrap values, a clustering of the
Australian human isolates into one uniform genetic group that was phylogenetically separated
from all the mouse isolates was well supported by biological data obtained in this study. To confirm the phylogeny of the C01 tree a small segment of the nuclear gene, paramyosin, was
sequenced in a number of isolates from humans and rodents. However, this gene did not
provide the level of heterogeneity required to distinguish between isolates from rodent and
human hosts. The high sequence conservation of the paramyosin gene characterised in this
study did not refute the finding that H. nana may be a cryptic species that is becoming host
adapted. It simply did not provide additional data to that already obtained.
A DNA fingerprinting tool, PCR-RFLP, of the ribosomal intergenic spacer (IGS), was
developed in this study in order to evaluate its usefulness in tracing particular genotypes within
a community, thus determining transmission patterns of H. nana between rodent and human
hosts. Analysis of the IGS of numerous H. nana isolates by PCR-RFLP identified the presence
of copies of the IGS that, whilst similar in length, differed in their sequence. Similar to that
observed in the ITS1, the existence of different IGS copies was found in both rodent and human
isolates of H. nana, thus the variability was not evidence of the existence of a rodent- or humanspecific
genotype. Evaluation of the intergenic spacer (IGS) as a fingerprinting tool suggests
that this region of DNA is too variable within individuals and thus, cannot be effectively used
for the study of transmission patterns of the tapeworm H. nana between different hosts.
In summary, it appears that the life cycle of H. nana that exists in remote communities in the
north-west of Western Australia is likely to involve mainly human to human transmission.
This is supported by both the biological and genetic data obtained for the mitochondrial locus in
this study. The role of the intermediate hosts, such as Tribolium spp., in the Hymenolepis life
cycle is still unclear, however it would appear that it may be greatly reduced in the transmission
of this parasite in remote Australian communities. In the future, it is recommended that further
genetic characterisation of faster evolving mitochondrial genes, and/or suitable nuclear genes be
characterised in a larger number of isolates of H. nana. The use of techniques which can
combine the characterisation of genotype and phenotype, such as proteomics, may also be
highly valuable for studies on H. nana from different hosts.
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Studies on the pathophysiology of parasitic diseaseSymons, Lawrence Eric Alexander Unknown Date (has links)
1v. (various pagings) : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (D.Sc.1974) from the Dept. of Entomology, University of Adelaide
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The sympatric coexistence of two reproductively independent lines of the endoparasitic wasp Venturia canescens / by Harry Leslie Scougall Roberts.Roberts, Harry L. S. January 2005 (has links)
"February 2005" / Includes bibliographical references. / 1 v. (various paging) : ill., plates ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / It has recently been shown that a thelytokous laboratory culture of the parasitoid wasp Venturia canescens contains two genetically distinct lines, coexisting on their host, the flour moth Ephestia kuehniella. The primary aim of the research presented in this thesis was to determine the basis of the coexistence of the two wasp strains, and estimate whether it is an artefact of the laboratory rearing conditions, or could, in principle, occur in field populations. / Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Discipline of Plant and Pest Science, 2005
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Urban Mobility: Transference and Atlanta's TransitFutrell, Janae Maegan 09 April 2007 (has links)
The research segment of this thesis creates the first comprehensive repository of the current and proposed elements of public transit that will potentially operate in Atlanta. Beginning with a base GIS map of Atlanta Regional Commissions [ARC] Regional Transit Plan Mobility 2030, other GIS shapefiles from MARTA and Georgia Regional Transit Authority [GRTA] were added to complete the map of what Atlantas public transit might soon become. Working within this framework, the analysis provides the potential locations for ten nodes of transference located within Atlanta and its outlying areas - all classified by their relative locations within the city. This thesis analyzes methods of connectivity within these nodes and attempts to arrive at successful conditions of transference between various transit modes; resulting in a series of conceptual design proposals that create both modular efficiency and a standardized aesthetic language.
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