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Phosphorus (P) is one of the three major macronutrients that plants need to grow and survive. When P is scarce, plants utilize a network of characterized responses known as the Phosphate Starvation Response (PSR) to remobilize internal stores of P as well as external P from soil. Emerging evidence shows the PSR is regulated by a specialized group of secondary messenger molecules, inositol pyrophosphates (PP-InsP). PP-InsPs and their precursors, inositol phosphates (InsPs), are important for plant abiotic stress responses, hormone signaling, and other stress responses. While PP-InsPs are critical for plant survival, much about the roles of PP-InsPs and how they are regulated remains to be understood. Further, the enzymes responsible for the synthesis of PP-InsPs in plants have been recently discovered; however, not much is known about the enzymes that degrade PP-InsPs in plants. The goal of the work presented herein is to understand critical aspects of the PP-InsP signaling in plants and leverage this information into a P phytoremediation strategy. To achieve this, I have investigated a group of PP-InsP phosphatases and assessed long-term impacts of depleting PP-InsPs in two plant species, Arabidopsis thaliana (Arabidopsis) and Thlaspi arvense (Pennycress). Exploring the impact of plant PP-InsP phosphatases has allowed me to explore critical aspects of PP-InsP sensing that show great promise for informing P remediation strategies. / Doctor of Philosophy / The Phosphorus (P) crisis presents a major challenge to food security. While Phosphorus (P) is critical for crop growth, P is a nonrenewable and increasingly limited resource. Our global population is fed at the expense of the remaining mineable P reserve, which may be depleted in as early as 30 years. Further, fertilizer runoff from farmland and urban areas poses a dangerous problem as increased nutrients in watersheds toxifies our water supply and aquatic ecosystems. Time is running out to preserve our P supply. New and innovative strategies that reduce fertilizer inputs and watershed pollution are key to securing the global food supply and protecting the environment. Emerging evidence shows plants sense and respond to P using signaling molecules known as inositol pyrophosphates (PP-InsPs). My work and that of others are key in showing that alteration of the levels of PP-InsPs can decrease plant P dependency or cause plants to hyperaccumulate P. Understanding how plants are able to sense, respond, and acquire P is crucial to inform future P phytoremediation strategies to secure global food security.

Phytoremediation is an attractive remediation technology due to its relative low cost and maintenance requirement. Acceptance of phytoremediation requires that the contaminant removal mechanisms are characterized and demonstrated in the field. Quantification of contributions from each mechanism to the overall remediation rate is crucial for optimization of phytoremediation systems, risk management and prediction of the total remediation time. The objective of this research was to characterize and quantify removal mechanisms for naphthalene at a creosote-contaminated site with poplar trees in Oneida, Tennessee. Groundwater monitoring for seven years in the surficial aquifer at this site demonstrated a reduction in polycyclic aromatic hydrocarbons (PAHs) with selective removal of naphthalene and three-ring compounds. Naphthalene mass loss mechanisms investigated at this site are biodegradation in the saturated zone, volatilization and biodegradation in the vadose zone and phytovolatilization. This is probably the most comprehensive field study of PAH phytoremediation mechanisms conducted to date. The significance of this research is to contribute to predictions of remediation time and end result for phytoremediation of PAHs. The understanding of in situ factors controlling each mechanism can facilitate future optimization of phytoremediation systems as well as improve risk assessment and monitoring strategies. Biodegradation rates were determined for different conditions at this site with in situ respiration tests, laboratory soil microcosms and laboratory soil columns. The combined remediation mechanisms of volatilization and biodegradation in the vadose zone were investigated in the field and in laboratory columns. Field measurements show that lower groundwater elevations in the summer and early fall lead to elevated groundwater concentrations of naphthalene and increased volatilization. The increase in the fraction of the porespace occupied by gas (gas saturation) in the unsaturated zone during the summer and fall further enhances the volatilization by increasing effective diffusion rates. Water consumption and interception by the phytoremediation system are believed to enhance mass transfer to the vadose zone. Column experiments and field measurements show that more than 90% of the naphthalene vapors are biodegraded within 5-10 cm above the groundwater table. The data indicate that biodegradation increases the overall volatilization flux out of the source by 10-300 times, when the source is exposed directly to the gas phase. In situ the naphthalene is generally dissolved from the source into the groundwater and then volatilezed from the groundwater to the gas phase. Under these conditions biodegradation in the vadose zone will still indirectly have an enhancing effect on the flux out of the source. This is the result of removal naphthalene from the soil gas by biodegrdation driving removal from the groundwater by volatilization, which in turn drives dissolution form the source into the groundwater. Phytovolatilization was quantified in flux chambers mounted on trees and calculated from transpiration rates. A laboratory uptake study and analysis of tree cores from the site provided supplementary evidence for naphthalene uptake by poplar trees. Phytovolatilization was detected throughout the year and was highest in the summer and fall when the groundwater concentrations were highest and transpiration was active. The role of biodegradation relative to physical removal mechanisms was compared for a year, for winter and summer conditions and with and without the impact of phytoremediation. Biodegradation of naphthalene in the saturated zone dominates by orders of magnitude over the removal by volatilization and phytovolatilization of naphthalene at this site. The removal of the total residual naphthalene mass was estimated to require up to 100 years with phytoremediation, but more than twice as long without phytoremediation. The estimated removal of naphthalene was three times larger in the summer than in the winter due to slower biodegradation in the saturated zone and smaller rates of volatilization to the vadose zone in the winter. The research shows that phytoremediation enhances the overall naphthalene removal, mainly by stimulating faster biodegradation in the rhizosphere and promoting mass transfer of naphthalene to the vadose zone followed by rapid vadose zone biodegradation. In the future, phytoremediation research focusing on the capillary zone is desirable. / Ph. D.

"Transparency- the ability to see into and understand the inner workings of a landscape- is an absolutely essential ingredient to sustainability" -Robert Thayer from "Green World, Green Heart" Current land filling practices that bury waste and debris below layers of earth and synthetic caps do not take into account the potential of reclamation of the site after the landfill debris has become stable. As development and consumerism increases, the need for land reclamation grows stronger, as earth will succumb to overabundance of human excessiveness. Can a space be created that not only reclaims land, but also exposes what is hidden- in order to educate the public on the importance of recycling and sustainability? Is it possible to design a space that addresses the issues and culture of the past, present and future, particular to a geographic site? Can landscape architects use landscape as an educational medium for self-discovery? / Master of Landscape Architecture

Floating treatment wetlands (FTWs) are an innovative best management practice that can enhance the performance of traditional retention ponds by increasing removal of the nutrients nitrogen (N) and phosphorous (P). FTWs consist of floating rafts on which wetland plants are planted, allowing the roots to be submerged below the water surface while the shoots remain above. A growing body of research has documented FTW performance with regard to urban runoff treatment, however evaluation of FTW effectiveness for treatment of agricultural runoff has received less attention. Due to high fertilization and irrigation rates, commercial nursery runoff contains much higher concentrations of N and P than runoff from urban areas. We conducted this study over two growing seasons (2015 and 2016) to assess the effectiveness of FTWs for use in commercial nursery retention ponds. In the first study we used two different nutrient concentrations, one to simulate nursery runoff (17.1 mg∙L-1 TN and 2.61 mg∙L-1 TP) and one to simulate concentrations that fall between urban and nursery runoff (5.22 mg∙L-1 TN and 0.52 mg∙L-1 TP). Four treatments were used: 1) Pontederia cordata planted in cups supported by a Beemat, 2) Juncus effusus planted in cups supported by a Beemat, 3) a Beemat with no plants, and 4) no treatment (open-water). Performance was evaluated based on a 7-day hydraulic retention time (HRT). Pontederia cordata removed between 90.3% and 92.4% of total phosphorus (TP) and 84.3% and 88.9% total nitrogen (TN), depending on initial loads. These reductions were significantly more than other treatments at both high and low nutrient loading rates. Juncus effusus performed better than the control treatments for TP removal at low nutrient concentrations, but did not perform any better than the control at higher nutrient loads. In the second study, conducted in 2016, we evaluated different plant species over two 8-week trials using simulated nursery runoff. We used five monoculture FTWs with the following species: Agrostis alba, Canna ×generalis, Carex stricta, Iris ensata, and Panicum virgatum. Additionally, two treatments were created from mixed species plantings and the final treatment consisted of an open water control mesocosm. Nutrient removal performance was evaluated over a 7-day HRT. P removal (phosphate-P) by FTW treatments ranged from 26.1% to 64.7% for trial 1 and 26.8% to 63.2% for trial 2. Trial 1 N removal (sum of ammonium-N, nitrate-N, and nitrite-N) efficiencies ranged from 38.9% to 82.4%, and trial 2 ranged from 12.9% to 59.6%. Panicum virgatum removed significantly more N and P than the control and any other FTW treatment in the second study. Both studies indicated, depending upon plant species, that FTWs can effectively remove nitrogen and phosphorous from urban and commercial nursery retention ponds. / Master of Science

Pollution of soil by trace metals has become one of the biggest global environmental challenges resulting from anthropogenic activities, therefore, restoration of metal contaminated sites needs due attention. The use of phytoremediation technologies as nature-based solution to pollution, could support successful implementation of green economic development strategies; with economically affordable and environmentally friendly benefits. The present investigation employed an exploratory study on the phytoremediation potentials of three selected native plants; Phytolacca dodecandra (L’Herit), Adhatoda schimperiana (Hochst) and Solanum incanum L, dominating areas close to heavy metal contamination sources; in metropolitan centers of Addis Ababa. In this work, concentration of six heavy metals of interest chromium (Cr), lead (Pb), cadmium (Cd), nickel (Ni) copper (Cu) and zinc (Zn) were examined in soil and in different tissues (leaves, stems and roots) of selected plants (both seedlings and mature plants), in dry and rainy seasons using atomic absorption spectrophotometer. Efficiency of phytoremediation is discussed based on calculated values of Bio-concentration Factor (BCF), Translocation Factors (TF) and Bioaccumulation Coefficient (BAC). Phytolacca dodecandra showed BCF, TF and BAC > 1 for Zn, Pb, Ni, Cu and Cd Adhatoda schimperiana gave BCF, TF and BAC > 1 for Zn, Cu, Ni and Cr; likewise, BCF, BAC and TF values of > 1 were noted in Solanum incanum for Zn, Cu, Pb and Ni. Based on these scenarios, the three plants could be utilized for phytoextraction of contaminated soil. Conversely, BCF and BAC for Cr levels in tissues of Phytolacca dodecandra were all < 1, which indicates unsuitability for phytoremediation of Cr in contaminated soils. Besides, Adhatoda schimperiana retained Pb and Cd in their roots showing root BCF > 1, while BAC and TF < 1, which highlights its suitability for phytostabilization. Moreover, BCF, TF and BAC values of < 1 noted for Cr and Cd in Solanum incanum reveal that Solanum incanum may not be a good candidate for remediation of Cr and Cd contaminated environments. In conclusion, results from this study revealed that the selected plants can accumulate substantial amounts of the above trace metals in their tissues and can serve as prospective phytoremediators of most of these metals. Phytoextraction and phytostabilization were the main mechanisms of remediation in this study. / Environmental Sciences / Ph. D. (Environmental Sciences)