Charles University Faculty of Pharmacy in Hradcec Králové Department of Pharmaceutical Technology Supervisor: doc. RNDr. Milan Dittrich, CSc. Student: Simona Medviďová Title of thesis: Formulation and testing of branched polyesters siRNA-loaded nanoparticles The progression of development increases demand for new and more effective drug systems formulations. This diploma thesis is focused on the preparation of nanoparticles from biodegradable branched polyesters based on PLGA, which are suitable for the transport of an oligonucleotide of a small interfering nucleic acid, siRNA. The theoretical part focuses on the characteristics of usable polymers, their possible modifications, the methods of preparation with closer look at the selected nanoprecipitation method, the important parameters such as particles size, zeta potential, and polydispersity. The main part is also characterization of siRNA in terms of structure, properties, modifications, and its function. A more extensive experimental part deals with the appropriate choice of type and concentration of polyester and stabilizer, methods of preparation and characterization of nanoparticles, and the possibility of analyzing encapsulated siRNA. Chapters results and discussion compares polyesters branched on tripentaerythritol, and polyacrylic acid...

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As part of the EU’s sustainable strategy in reducing emissions and combating climate change the EU introduced the Corporate Sustainability Reporting Directive (CSRD) in 2022. This directive requires the reporting of sustainability information from a significantly wider group of companies. The CSRD imposes higher demands on reporting and especially within a company’s Scope 3 transportation emissions. The study aims to identify and understand the challenges newly affected companies face in collecting and reporting scope 3 transportation emissions to comply with the CSRD. The study also intends to shed light on the resource and capability gaps that newly affected companies encounter and propose strategies and solutions to address these challenges. The study draws from institutional theory combined with are source-based view of a company to propose a hypothesis that companies face resource and capability gaps due to institutional pressure. The research methodology employed was exploratory multi-method qualitative research. The findings identified how companies, needing to adapt to the CSRD, perceived a regulatory institutional pressure in the form of new and higher requirements and demands now placed on them. In addition the exploration resulted in identification of external challenges, affecting companies ability to comply with the new legalization. Furthermore, internal and supplier challenges were discovered as factors within companies that affect their compliance. These highlighted the presence of resource and capability gaps within companies. In order to close these gaps, the study proposes several strategies and solutions together with a roadmap to address these challenges, ultimately guiding companies towards managing institutional pressures and enhancing their sustainable competitive advantage. Solutions regarding management and strategy as well as tangible operations were identified. These solutions and strategies include developing a sustainability culture within the organization, engaging with stakeholders through collaboration and communication efforts, and implementing technology solutions for data collection and analysis. The study can potentially be utilized by companies to navigate useful solutions when trying to comply with the CSRD. Additionally, the study contributes to guiding companies towards a future position of managing institutional pressure while enhancing their sustainable competitive advantage. Companies are encouraged to shift and transform their viewpoint of complying with the institutional pressure of CSRD from a compliance-focused approach towards a corporate social responsibility-driven sustainable approach with long-term value development. The study thus contributes with a new perspective by highlighting how complying with sustainability reporting can become a tool for advancing corporate responsibility and contributing to a more sustainable development and responsible business landscape.

Made available in DSpace on 2016-08-10T10:55:15Z (GMT). No. of bitstreams: 1 Paulo Lopes da Silva.pdf: 2017866 bytes, checksum: 9d8986584beaa350fa0d44c75ba8bf36 (MD5) Previous issue date: 2006-08-16 / The increasing quality lost of underground water all over the world, due to intensified antropic action during many decades, may make impracticable the future use of this natural resource. The modernity including its promise of adventure, power, joy, growth, interior change and change of the things around it (...) is at the same time the threat to all we have, all we know, all we are (Berman, 1988). According to this idea, the environment issue claims the problematicals consequences of the modernity and capitalist dynamics. Many are the environment problems, such as the freatic water pollution. These resources already supply more than 50% of the world population with water. The way today we face the environment problem and the way we understand them, its instruments, its politics such as all the dynamic brought in the search of its confrontation, are part of the big questions of this century. The freatic water environment problems occurs whenever more people demand higher life style standard with cheaper technologies, even if the involuntary sub products include the soil degradation, the toxic polluter, the animal species´ extinction or the climate changes. The environment is the totality of physiographic factors (soil), water, forest, relief, geology, landscape, meteorological factors and climate factors added to psycho-socials inherent to human nature (such as behavior, well-being, mind spirit, job, health, food) added to sociological factors such as culture, civility, sociability, respect and peace. So the planet Earth should be considered as a unique system. As we expand our understanding about the system which controls the environment and its never-ending interconnections, potential solutions will consolidate. The study of freatic water systems capacity/potentiality and natural geosystems as support to human needs will be a way of consolidating the scientific knowledge. It will do the necessary inter-relation between the natural environment and human potentiality. This way, an increasing consciousness related to environment issue appears as the possibility of the union between human and nature, both can t be analyzed as excludents poles. In the next pages, Goiânia and Aparecida de Goiânia cities are explore about yours underground water. / A crescente perda da qualidade das águas subterrâneas em todo o mundo, devido à ação antrópica intensificada ao longo de muitas décadas, pode inviabilizar a utilização futura desse recurso natural. A modernidade dentro de sua promessa de aventura, poder, alegria, crescimento, autotransformação e transformação das coisas ao seu redor (...) é ao mesmo tempo ameaça a tudo que temos, tudo o que sabemos, tudo o que somos . Nesse sentido a questão ambiental evoca as conseqüências problemáticas da modernidade e da dinâmica capitalista. Vários são os problemas ambientais, entre eles a poluição dos lençóis freáticos, que já abastecem mais de 50% da população mundial, a maneira que hoje enfrentamos, a circunstância como se dá esse entendimento, de seus instrumentos, de suas políticas, assim como toda a dinâmica na busca de seu enfrentamento fazem parte das grandes questões do nosso século. Os problemas ambientais com os lençóis freáticos surgem porque cada vez mais pessoas exigem padrões de vida mais altos com tecnologias mais baratas, mesmo que os subprodutos involuntários incluam a degradação dos solos, os poluentes tóxicos, a extinção de espécies animais ou alterações climáticas. Assim o meio ambiente sadio, ou seja, a totalidade dos fatores fisiográficos tais como o solo, a água, a floresta, o relevo, a geologia, a paisagem, fatores meteoros-climáticos mais os fatores psicossociais inerentes á natureza humana (comportamento, bem-estar, estado de espírito, trabalho, saúde, alimentação, etc.) somados aos fatores sociológicos como cultura, civilidade, convivência, o respeito, a paz etc. é que iluminará a sobrevivência humana. Dessa forma, planeta Terra deve ser visto como um único sistema e na medida em que expandirmos nossa compreensão sobre o sistema que controla o meio ambiente, das suas infinitas interligações, soluções potenciais se consolidarão. O estudo da capacidade/potencialidade dos sistemas freáticos e geossistemas naturais, como suporte às necessidades humanas, é ou será uma forma de consolidar o conhecimento cientifico e que dará a inter-relação necessária entre o meio natural e as potencialidades humanas. Desse modo, uma crescente conscientização acerca da questão ambiental, surge como a possibilidade de união entre o HOMEM e a NATUREZA, que não podem ser tomados como pólos excludentes. Nas páginas seguintes, exploram-se as condições ambientais, especialmente o lençol freático subterrâneo das cidades de Goiânia e Aparecida de Goiânia.