In my Master thesis I summarize up-to-date information on anorexia nervosa. In the theoratical part I introduce casuistics of young females suffering from anorexia nervosa. In the practical part I present the evaluation of research data dealing with particular risk factors that may lead to the development of anorexia nervosa in students of lower stage of grammar school.

This thesis deals with the issue of eating disorders of pupils attending upper primary schools of rural and urban type. The issue of eating disorders is very complex and public often fails to understand it. People do not see the fact that the food and its rejection can turn into a real habit. Eating disorders are not only observed in young women, but also increasingly in children and adolescents. Eating disorders involve serious psychosomatic disorders, significantly affecting the mental and physical status. Thus, they may very negatively affect personality development from childhood to adulthood. They are accompanied with many somatic problems and complications and they can have a very negative influence to the overall health status of an individual. Eating disorders cause long-term problems not only to patients themselves but also to their families, spouses and friends. It is very easy to fall ill with eating disorder, but is very hard to get better.

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Ghrelin analogs in food intake regulation Ghrelin, to date the only known peripherally produced and centrally acting peptide that stimulates food intake, is mainly synthesized in the stomach and acts through growth- hormone-secretagogue receptor (GHS-R). In addition to its orexigenic effect, ghrelin stimulates growth hormone (GH) release, gastric motility and acid secretion. The diverse functions of ghrelin raise the possibility of its clinical application for GH deficiency, eating disorders, and gastrointestinal diseases. Ghrelin agonists could be a promising therapeutics in cachexia occurring at cancer or chronic inflammatory diseases. Octanoylation of Ser3 is crucial for preservation of ghrelin activity. In this study, biological properties (binding to GHS-R, food intake stimulation in mice) of full length and shorter ghrelin analogues with octanoic acid coupled to diaminopropionic acid (Dpr) replacing Ser3 or without octanoic acid were followed. This substitution resulted in a prolonged stability and orexigenic effect of above mentioned ghrelin analogues. Importance of N-terminal part of ghrelin and octanoylation peptide was also confirmed.

Prolactin-releasing peptide (PrRP) is a member of the family of RF-amide peptides. These peptides have typical C-terminal sequence -Arg-Phe-NH2 and similar biological effects. PrRP was discovered as an endogenous ligand of an orphan receptor GPR10 while searching for a factor responsible for a prolactin secretion. This effect was not later confirmed and nowadays, PrRP is mainly considered as an anorexigenic peptide. This is supported by a fact that PrRP and GPR10 deficient mice suffer from hyperphagia and late-onset obesity. Besides GPR10, PrRP is bound to NPFF2 receptor whose endogenous ligand is neuropeptide FF (NPFF). In this study, the PrRP's analogues modified at the N-terminus with fatty acids of different lenghts were tested in vitro on binding and activation MAPK/ERK1/2 signalling pathway. In in vivo experiments on food intake, the central anorexigenic effects of lipidized PrRP-analogues were tested provided their crossing blood brain barrier. Binding studies showed that all analogues bound to rat pituitary RC-4B/C cells with high affinity, analogues containing fatty acid with Ki of one order of magnitude lower than native PrRP. High affinity was also confirmed for binding to cells overexpressing GPR10 receptor and cell membranes with overexpressed NPFF2 receptor. All tested analogues...

The diploma thesis is focused on the one hand on the ways of the coordination of the multidisciplinary teams and the interdisciplinary cooperation of the professionals helping people with eating disorders (EDs) and on the other hand on the challenges that these professionals are facing and what are their solutions. The findings of the empirical part are based on a qualitative research using mostly semi-structured interviews with 17 professionals. Most of the professionals from the nonprofit type of organizations and from the treatment facilities had one permanent coordinator and they used regular team meetings as a part of the coordination. On the other hand the professionals with their private practice cooperating with each other were choosing the coordinator case by case or had none at all and they shared the information mostly irregularly by phone or email. The most frequent challenges of this cooperation were its time, financial, energy and coordination consuming character, insufficient knowledge or experience of some other professionals regarding EDs or their unwillingness to cooperate, myths about EDs or a disharmony among the cooperating professionals. The recommendations for practice are: a careful selection of the colleagues according to their competence, similar views and willingness to...