It has been shown previously in our lab that mutations in the pyrimidine pathway reduced the ability of Pseudomonas aeruginosa to produce virulence factors. Knockout mutations in pyrB, pyrC and pyrD genes of the pyrimidine pathway showed that virulence factor production was decreased. Pyoverdin, pyocyanin, hemolysin, iron chelation, motility, and adherence are all considered virulence factors. Here I further investigate the effects of mutations in the pyrimidine pathway by studying a pyrE mutant. I studied the effect of the pyrE mutation on the production of the above virulence factors. Just like the effect of pyrB, pyrC and pyrD mutations,the pyrE mutation also showed that the bacteria were deficient in producing virulence factors when compared to the wild type. The broader impact of this research would be the possibility of finding drugs that could treat patients infected with P. aeruginosa and possibly extend the lives of chronically infected patients with cystic fibrosis.

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This essay concerns the osteological cremated skeletal remains from Broe, Halla parish, Gotland of  The Late Iron Age. The focus of this essay is to determine the position of the corpse on the pyre, whether the cremated bones were crushed after or prior to the cremation and if the individual was buried in the pyre. In addition the species, age, sex and skeletal abnormalities of the bones in question will be studied. The result of the research has yielded that the cremated bones did not need to be crushed to result in the small fragment sizes. Crushing of the bones may have happened during the cremation, this conclusion has been drawn through the comparison of a pyre experiment were bone fragmentation is evident with cremated foxes, and dogs in the cremation graves from Broe. The construction of the pyre and the position of the corpse could not be determined through colouring and fragmentation of the bones. The human's were commonly buried in the pyre, and in some cases it is possible that the "grave" in fact is the remainder of a pyre. The species found are dog, horse, cow, sheep/goat and bear. The unburnt human bones in the cremation graves come from children. Of the cremated human’s, ages range from 18-44 to 50-79 occur. Three probable men and two probable women have been estimated, but there is no clear segregation between the sexes on the grave field, which is evident on some medieval church graveyards. Skeletal abnormalities in humans are found on the cranium, more specific senile osteoporosis, and on the dog’s osteophytosis.

Pseudomonas aeruginosa are medically important bacteria that are notorious for causing nosocomial infections. To gain more knowledge into understanding how this organism operates, it was decided to explore the pyrimidine biosynthetic pathway. Pyrimidine synthesis, being one half of the DNA structure, makes it a very important pathway to the organism’s survivability. With previous studies being done on various genes in the pathway, pyrE has not been studied to the fullest extent. To study the function of pyrE, a site directed mutagenesis was done to completely knock out pyrE, which encodes the protein orotate phosphoribosyl transferase that is responsible for converting orotate into orotate monophosphate (OMP). A mutation in this step leads to accumulation and secretion of orotate into the medium. Analyzing virulence factors produced by the mutant and comparing to the wild type, some intriguing features of the mutant were discovered. One of the findings was the over expression of virulence factors pyoverdin and pyocyanin. Pyocyanin over expression, based on the results of this study, is due to the accumulation of orotate while over production of pyoverdin is due to the accumulation of dihydroorotate. The other virulence factors studied were motility assays, exoproducts, and growth analysis. All virulence factor production was reduced significantly in the mutant compared to the wild type. The casein protease assay showed absolutely no production of proteases in the mutant. The conclusion is that orotate accumulation leads to a significant reduction in virulence factor production in Pseudomonas aeruginosa. In addition to that, it was found that excess orotate in the wild type led to a decrease in quorum sensing regulated products.

L’étude du bûcher funéraire fondée sur une approche anthracologique, s’inscrit dans la continuité d’unerecherche orientée vers la compréhension de l’organisation sociale des sociétés anciennes. La pratique dela crémation met en oeuvre une succession d’actions qu’il est nécessaire d’identifier pour enrichir nosconnaissances. Au travers de l’étude des bûchers en fosse individuels sous un angle anthracologique,deux objectifs ont été proposés : le premier porte sur un regard critique des données produites par lesanalyses anthracologiques en vu de proposer des catégories d’informations de premier ordre à prendre encompte lors d’études sur les structures de crémation. Le second, proprement archéologique est lié à larestitution du bûcher funéraire abordée sous l’angle de sa chaîne opératoire afin de discuter des moyensmis en oeuvre qui, d’un point de vue technique ou rituel, jalonne la conduite de la crémation. Pourrépondre à ces objectifs trois approches ont été développées.La première, anthracologique, a porté sur l’étude de dix-neuf structures de crémation du quart sud-est dela France, dix-sept appartenant à l’Antiquité, deux à la période protohistorique. L’étude de ces structuresa vu la mise en place de protocole de prélèvements et d’analyses permettant l’obtention de donnéesfiables pour une interprétation des gestes et pratiques funéraire.La deuxième, historique, propose une lecture inédite et approfondie des représentations de bûcher dansl’Antiquité, sur lesquelles un regard d’archéoanthracologue a été porté. Puis une relecture de sourcestextuelles connues et commentées, en mettant à profit nos connaissances techniques sur le sujet, enprenant le parti de vérifier, et parfois de modifier, les traductions tout en prenant soin de recontextualisercertains textes, a mis au jour des détails techniques relativement précis.La troisième approche se veut résolument accès sur la technique, il en ressort une description de la chaîneopératoire et une mise en évidence des moyens engagés pour réaliser une crémation. La conduite de lacrémation peut être vue comme un enchainement d’opérations techniques et rituelles qui ont été en partiecaractérisées par nos analyses anthracologiques. / The study of the funeral pyre based on an anthracological approach, is part of the continuity of a research oriented towards understanding the social organization of ancient societies. The practice of cremation implements a succession of actions that it is necessary to identify, to increase our knowledge. Through the study of individual pyres from an anthracological angle, two objectives have been proposed: the first concerns a critical look at the data produced by charcoal analyzes in order to propose categories of information to be taken in studies of cremation structures. The second, archaeological, is related to the restitution of the funeral pyre approached from the angle of its "chaîne opératoire" to discuss the ways implemented which, from a technical or ritual point of view, punctuates the conduct of cremation. To meet these objectives, three approaches have been developed. The first, anthracological, focused on the study of nineteen cremation structures in the south-east of France. , allowing the establishment of a protocol for sampling and analysis. The second, historical, offers a new and in-depth reading of the representations of the pyre in antiquity and textual sources on which a look of archaeanthracologist was carried. The third approach on the technique, proposes a description of the "chaîne opératoire" and a demonstration of the means committed to achieve a cremation.

The crematory funerary rites practiced by those living in parts of mainland Britain during the first millennium AD included burning complete or parts of animals on the pyre. This thesis highlights the potential for archaeozoological analysis of faunal pyre goods using assemblages from the first millennium AD as a dataset. Experimental study and the integration of current research from a number of disciplines is used to suggest that although pyrolysis and cremation practices fragment and distort burnt bone assemblages, careful analysis can reveal a wealth of data leading to the interpretation of various forms of pyre good. The results of the author's analysis of material from the sites of Brougham, Cumbria, St. Stephen's, Hertfordshire, Castleford, West Yorkshire and Heath Wood, Derbyshire are combined with data from other published cemeteries to suggest a series of chronological and regional continuities in the use of animals but with a distinct change at the start of the Early Medieval period. The results from Brougham are particularly significant as they alter preconceived views on the utilisation of animals in Romano-British funerary practice. Cremation burials in first millennium AD Britain are shown to include the burnt remains of predominantly domestic taxa with occasional wild species. The pyre goods are interpreted as representing food offerings, companions, amulets, gaming items and sacrifices. This thesis demonstrates that cremated animal bone should not be disregarded but rather valued as source of archaeozoological data, and a significant functional tool for interpreting past funerary behaviour and animal utilisation.

The crematory funerary rites practiced by those living in parts of mainland Britain during the first millennium AD included burning complete or parts of animals on the pyre. This thesis highlights the potential for archaeozoological analysis of faunal pyre goods using assemblages from the first millennium AD as a dataset. Experimental study and the integration of current research from a number of disciplines is used to suggest that although pyrolysis and cremation practices fragment and distort burnt bone assemblages, careful analysis can reveal a wealth of data leading to the interpretation of various forms of pyre good. The results of the author¿s analysis of material from the sites of Brougham, Cumbria, St. Stephen¿s, Hertfordshire, Castleford, West Yorkshire and Heath Wood, Derbyshire are combined with data from other published cemeteries to suggest a series of chronological and regional continuities in the use of animals but with a distinct change at the start of the Early Medieval period. The results from Brougham are particularly significant as they alter preconceived views on the utilisation of animals in Romano-British funerary practice. Cremation burials in first millennium AD Britain are shown to include the burnt remains of predominantly domestic taxa with occasional wild species. The pyre goods are interpreted as representing food offerings, companions, amulets, gaming items and sacrifices. This thesis demonstrates that cremated animal bone should not be disregarded but rather valued as source of archaeozoological data, and a significant functional tool for interpreting past funerary behaviour and animal utilisation.

Ce travail a pour fondement l’étude de trois ensembles funéraires périurbains situés dans les capitales de cité des peuples Médiomatriques (Metz-Divodurum), Nerviens (Bavay-Bagacum) et Morins (Thérouanne-Tervanna). Notre étude est basée sur un corpus composé de plus de 480 structures funéraires, auquel ont été ajoutées les données issues des autres découvertes funéraires effectuées dans ces trois villes, du 18ème siècle à nos jours. Sur la base de cette documentation, nous proposons une analyse des pratiques funéraires en usage dans le nord de la Gaule et plus particulièrement dans la province de la Gaule Belgique et de leur évolution au cours des trois premiers siècles de notre ère. La première partie de ce travail est consacrée à l’organisation des espaces funéraires en contexte urbain à travers l’analyse de différents paramètres : lieux et dynamique d’implantation des aires funéraires, organisation interne et structuration de l’espace ainsi que le mode de répartition des structures (fonctionnelle, chronologique et sociale). Les chapitres suivants sont consacrés à la compréhension et à l’interprétation des différentes pratiques et gestuelles funéraires qui ont pu être observées lors de l’étude des structures de notre corpus. Pour ce faire, nous avons tenté de restituer les différentes étapes du processus funéraire et de replacer l’ensemble de ces pratiques dans l’ordre selon lequel elles se sont déroulées. Dans un premier temps, nous nous sommes intéressées aux données relatives à l’étape de la crémation du corps observées lors de la fouille des bûchers funéraires, à travers l’examen de la structure des bûchers, des modalités de traitement du corps ainsi que du rôle et de l’origine du mobilier funéraire utilisés autour du bûcher. Ensuite, nous avons abordé le lieu de dépôt définitif des restes en présentant les éléments constitutifs de la tombe, les différents types de structures funéraires auxquels nous avons été confrontés ainsi que les divers modes de dépôts des ossements et leur organisation au sein de la fosse sépulcrale. Une attention particulière a également été portée aux étapes relatives au transfert et aux modalités de dépôt des restes osseux du défunt dans la tombe ainsi qu’aux pratiques et aux gestuelles qui en découlent. Enfin, dans un dernier chapitre, nous avons analysé les différents apports du mobilier funéraire afin de mieux cerner les différents gestes, perceptibles à travers les restes archéologiques, effectués lors de la constitution de la tombe mais également tout au long des funérailles ou encore lors de fêtes commémoratives où l’on rendait hommage au défunt. Cette étude synthétique s’appuie sur un catalogue détaillé des sépultures, composé de deux volumes (volume 1 : Metz « Avenue André Malraux », volume 2 : Bavay « La Fache des Près Aulnoys » et Thérouanne « Les Oblets ») présentant, par phases chronologiques, les observations de terrain, l’inventaire du mobilier, ainsi que les résultats des études des restes osseux humains et animaux. / This work is founded on the study of three funerary complexes of outlying suburbs located in the capital cities of Mediomatrici (Metz-Divodurum), Nervii (Bagay-Bagacum) and Morini (Thérouanne-Tervanna). Our study is based on a corpus composed of more than 480 funerary structures, which has been amended by the data issued from the other funerary discoveries carried out in these three cities, from the 18th century to nowadays. Based upon that documentation, we propose an analysis of funerary practices in use in the North of Gaul and more particularly in the province of Gallia Belgica and of their evolution in the first three centuries of our area. The first part of this work is related to the organization of the funerary areas in an urban context through the analysis of different parameters: places and settlement dynamic of funerary areas, internal organization and space structuring as well as the mode of distribution of the structures (functional, chronological and social). The following chapters are related to the understanding and interpretation of the different funerary practices and gestures, which may have been observed during the study of the structures of our corpus. To do that, we have tried to reconstruct the different steps of the funerary process and to replace the whole practices in the order in which they took place. First, we took an interest in the data connected with the stage of the body cremation, data observed during the excavation of the funerary pyres, through the examination of the pit structure, the methods of treatment of the body as well as the role and the origin of the grave goods used around the pyre. Then, we moved on to the place of final deposition of the remains by presenting the constituent elements of the grave, the different types of funerary structures we have been confronted with as well as the varied modes of deposition of the bones and their organization within the sepulchral pit. A specific attention has also been given to the steps which are relative to the transfer and to the modes of deposition of the bones of the deceased in the grave as well as the practices and body movements which follow that. Finally, in a last chapter, we have analyzed the different deposits of the funerary goods in order to define as well as possible the different gestures which were pointed out through the archaeological remains, and which were made at the time of the composition of the grave but also throughout the funeral or during the memorial feasts where respects to the deceased were paid. This overall study is based on a detailed catalogue of the tombs which is composed of two volumes (volume 1: Metz “Avenue André Malraux”, volume 2: Bavay “La Fache des Près Aulnoys” and Thérouanne “Les Oblets”) displaying by chronological phases the observations made during the excavation work, the inventory of the grave goods, as well as the results of the studies of animal and human bones rests. / Diese Arbeit befasst sich mit drei am Stadtrand gelegenen Grabensembles, die in den civitates-Hauptstädten der Mediomatriker (Metz-Divodurum), Nervier (Bavay-Bagacum) und Moriner (Thérouanne-Tervanna) liegen. Unsere Untersuchungen beziehen sich auf eine Sammlung von mehr als 480 Grabstrukturen, ergänzt durch Grabungsdokumentationen früherer Entdeckungen, die von 18. Jh. bis heute in diesen drei Städten zu Tage gekommen sind. Anhand dieser Dokumentation, ist eine Analyse der im Nordgallien und besonders in Gallia Belgica üblichen Bestattungsbräuche und deren Entwicklung während der drei ersten Jahrhunderte nach Christus möglich. Der erste Teil dieser Arbeit ist den Grabbereichen im Stadtbereich gewidmet, wobei verschiedener Faktoren untersucht worden sind: Orte und Ansiedlungsdynamik der Grabareale, innere Organisation und Raumstrukturierung sowie Verbreitungsart der Befunde (funktional, chronologisch und gesellschaftlich). Die folgenden Kapitel sind dem Verständnis und der Interpretation der verschiedenen Bestattungsbräuche und –gesten, die während der Studie der Befunde unseres Corpus beachtet wurden, gewidmet. Dabei wurde versucht, die verschiedenen Etappen des Bestattungsprozesses, in der Reihenfolge wie sie vollzogen wurden, zu rekonstruieren. Zuerst haben wir uns für die in den Grabungen beachteten Gegebenheiten der Kremationsetappen der Körper interessiert, durch Untersuchung der Scheiterhaufenstruktur, Behandlungsbedingungen der Körper sowie Rolle und Herkunft der Grabbeigaben, die um die Scheiterhaufen benutzt wurden. Dann haben wir die Stelle, die der endgültigen Deponierung des Leichenbrandes diente, angesprochen, durch Vorstellung der Grabgrundlagen, der verschiedenen Grabstrukturtypen mit welchen wir konfrontiert waren, sowie der diversen Formen der Knochendeponierungen und ihre Organisation innerhalb der Grabgrube. Ausserdem wurden die Etappen, die im Zusammenhang mit dem Transfer und den Deponierungsbedingungen des Knochenmaterials ins Grab stehen, sowie die Bräuche und Gesten die daraus folgen, besonders analysiert. Im letzten Kapitel wurden schließlich die verschiedenen Elemente des Grabmaterials untersucht, um die diversen durch archäologischen Gegebenheiten erkennbaren Gesten besser abgrenzen zu können, welche während der Komposition des Grabes, während des Begräbnisses selbst oder aber während der Gedenkfeiern, durchgeführt wurden. Diese zusammenfassende Arbeit stützt sich auf einem detaillierten Katalog der Gräber, eingeteilt in zwei Bände (Band 1: Metz « Avenue André Malraux », Band 2: Bavay « La Fache des Près Aulnoys » und Thérouanne « Les Oblets »), in welchem die Grabungsbeobachtungen, die Grabinventaren sowie die Ergebnisse des Studiums des menschlichen und tierischen Knochenmaterials in chronologischer Reihenfolge präsentiert sind.