This diploma thesis deals with non-native species of crustaceans, which are already present not only in Europe but also successfully inhabit the territory of the Czech Republic. The species that this work deals with is the signal crayfish (Pacifastacus leniusculus), marbled crayfish (Procambarus fallax f. virginalis) and the killer shrimp (Dikerogammarus villosus). The main goal of this study was to demonstrate the ability of killer shrimp to attack and prey on the crayfish eggs and hatchlings of above mentioned species, which were used not only for theuir presencein Czech Republic but also as model representatives of families Astacidae and Cambaridae. It was therefore the confirmation of high aggressiveness of killer shrimp and point out, that this species is capable of negatively influencing even the bigger invertebrates by direct predation on their early developmental stages. Another aim was to assess whether the killer shrimp can be a suitable prey for a crayfish. As the experiments have shown, killer shrimp is able to predate on crayfish eggs and hatchlings even directly on females abdomens wehre they are incubated and protected. The results confirmed the theory of killer shrimp hazards to aquatic ecosystems and its potential to directly influence the populations of larger organisms. At the same time, it has been found that killer shrimp may be a suitable prey for adult and subadult individuals of signal crayfish and adults of marbled crayfish.

The study is focused on the evaluation of crayfish physiological reaction on hygienically treated water with chlorine dioxide (ClO2). A patented non-invasive monitoring system was used for observation of crayfish cardiac and locomotor activity. Monitoring was conducted from February to August 2017 under conditions of private commercial enterprise "Pivovar Protivín" in Czech Republic. Adult individuals of signal crayfish, Pacifastacus leniusculus, were kept separately in water-flow aquariums directly after the water treatment device producing ClO2 in concentration from 0.01 to 0.29 mg.l-1. Observed crayfish response to the disinfectant varied among individuals which could be explained by a different functional state and individual reaction on stimuli. Diurnal rhythm of some crayfish was disturbed even at a lower concentrations of chlorine dioxide (0.01-0.2 mg.l-1), while higher concentrations affected all animals. In addition to that, higher levels of chlorine dioxide ( 0.2 mg.l-1) significantly increased mortality. Maximum concentrations (0.2-0.29 mg.l-1) were observed 28 times in total during 202 days of monitoring, which resulted in 25 mortality cases occurred several days after exposure. In average, mortality of crayfish occurred three-four weeks after stocking to the experimental system. Possible lethal concentration of ClO2, which caused animal mortality, is 0.2 mg.l-1. Results suggested that crayfish exposure to ClO2, obviously, negatively affect their physiological processes; however, further studies are needed to examine specific effects of chlorine dioxide on internal organs of crayfish.

The diploma thesis observed the indicators of growth in individual developmental stages of our native crayfish (Astacus astacus, Austropotamobius torrentium, Astacus leptodactylus) and non-native crayfish (Pacifastacus leniusculus, Orconectes limosus) under experimental conditions. The next aim of work was to describe the time of hatching of these species, the moulting interval, the frequency of moulting and increase after individual moulting during the first vegetation season in two different temperatures (15{$\rm^o$}C a 21{$\rm^o$}C).

Main purpose of this diploma work was to find out possibility using PIT telemetry in monitoring strange crayfish species Spinycheek crayfish Orconectes Limosus. Research of the theme consisted of three steps: two laboratory researches and one research in the natural environment. The first laboratory study concerned tagging passive integrated transponder (PIT) to various part of crayfish body. PIT tags were internally implanted to cephalothorax, abdomen and claw. The highest mortality was in tagging to claw. The second laboratory research consisted of comparing different tagging methods to PIT telemetry. Crayfish were tagging by PIT telemetry, soldering, visible implant elastomers and visible implant Alpha tags. Among tagging groups there were not to find out different in mortality. In the third research the crayfish were tagging in their natural environment. There were using three methods: PIT telemetry, soldering and VI Alpha tags. This experiment wasn´t successful. During recapturing crayfish there were not to find any tagging crayfish.

Astacologic survey took place in ÚN Lipno and was conducted in time from 2012 till 2013. For the actual mapping of the occurrence of spiny-cheek crayfish, the locations of total 17 places were detected. These monitoring areas were carried out in two ways for collecting the samples. The trapping of all individuals was achieved with special instruments, like pots or traps by hands or with the help of nets. For the caught specimens of spiny-cheek crayfish, which were determined by sex, the length of carapace was measured and as well if possible, the postorbital carapace length was taken. Throughout the whole monitoring, 228 individuals of spiny-cheek crayfish were caught in the amount of 107 (47%) females (CL = 21.5 ? 7.3 mm) and 121 (53%) males (CL = 20.1 ? 5 mm). Another aim of this work was to demonstrate that crayfish figure in fish food menu. Catches of fish were carried out in two ways, using fishing rods or into the clutches. The stomachs and intestines contents were analyzed at caught fish samples. If the condition of the contents of stomachs or intestines were allowed, it was also identified, what kind of food it was. For our annual issues, were included mainly species of piscivor fish. Spiny-cheek crayfish were identified in the stomachs of two species of fish, namely perch and pike.

In the nature, crayfish are represented like predators or a part of food base of some fish. Also in free waters they acts as detrivors and are an important element in the nutrient and energy circle in aquatic ecosystem. The crayfish weren't able to avoid negative impacts of anthropogenic activities such as water pollution and devastation of habitats. For these reasons reproduction of the crayfish is necessary in hatcheries and farms. The main aim of my work was to study the reproductive biology of the crayfish focused on the process of mating, the interval between mating and laying eggs, fertilization and morphological structure of the male and female gametes. Among the secondary objectives there was included using of repeated electrostimulation of the crayfish and the possibility of hybrids of the noble crayfish (Astacus astacus) and the narrow-clawed crayfish (Astacus leptodactylus). The observation of the period of reproduction was realized on representativies of the noble crayfish and the narrow-clawed crayfish throughout natural mating in the tanks. The structure of gametes was observed on the samples taken from thenoble crayfish (Astacus astacus), the narrow-clawed crayfish (Astacus leptodactylus), the signal crayfish (Pacifastacus leniusculus), the red swamp crayfish (Procambarus clarkii) and spinycheek crayfish (Orconectes limosus). The experiments were carried out during the autumn season in 2012 and 2013. The results of observations of the reproduction of crayfish made us clear as for the period and intervals for all phases of the process of mating crayfish. For females the noble crayfish and the narrow-clawed crayfish was found oviposition without the presence of males. Their individual components have been described by using ultrastructure of males and female gametes. Repeated electrostimulation showed at the family Astacidae as a suitable tool for collecting large mass of sperm material. Hybridization experiment proved that it can occur to mate the two species of crayfish in areas where they occur simultaneously. These experiments helped to further knowledge of reproductive biology of crayfish.

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Approved for public release, distribution is unlimited / This thesis explores the ongoing Malay-Muslim separatist insurgency in southern Thailand. In particular, it argues that historically-rooted structural factors, to include relative economic deprivation, limited political integration, and struggle for the maintenance of ethnic-religious identity, are at the root of this insurgency. The year 2001 produced two catalysts for the renewal of this insurgency, one internal and one external. The internal catalyst was a newly elected suppressive government regime under the leadership of Prime Minister Thaksin and the Thai Rak Thai Party. The external catalyst was the growing, increasingly radicalized Islamist movement, largely defined through terrorist violence, that expressed itself in the 9/11 attacks. The combination of these has produced rekindled secessionist violence of a previously unknown level in the Thai provinces of Pattani, Narathiwat, and Yala. Given the deeply ingrained structural cause of this insurgency, as well as a government administration whose policies and conflict mismanagement continually fuels the violence, the prospect for conflict resolution is not promising. Nonetheless, it remains in the best interests of the United States that this conflict is soon resolved. Should the insurgency continue growing, the situation may reach a point of drastic consequences for Thailand as well as the United States. / Captain, United States Air Force

The "Zákolanský potok" takes part in European nature program Special Area of Conservation because of the presence of stone crayfish (Austropotamobius torentium), which is listed as threatened species. The area around brook is intensively agriculturally cultivated. Water from wastewater treatments plants is drained to the local brooks. The aim of the work was to describe diversity of macrozoobenthos on the brook called "Zákolanský potok". Further aim was to evaluate the ecological condition of the watercourse using biotic indexes. The samples of macrobenthos were taken from three profiles in five sampling periods. Profile 1 was control, profile 2 was under the wastewater treatments plants and the third profile was placed down the stream. Diversity, biomass, saprobic index, BMWP, and ASTP scores were evaluated for the community of macrozoobenthos. The saprobic index was evaluated to the beta-mezosaprobity value (2.0-2.4) in the first profile, the saprobic index on the second profile showed a relatively wide range from worse oligosaprobity to worse beta-mesosaprobity (1.2-2.4). On the third profile, the saprobic index ranged from better beta-mesosaprobity to better alpha-mesosaprobity (1.6-2.6). The BMWP and ASPT scores in all profiles refer to medium to low quality aquatic environments. The diversity of the community gained the best values on the 3rd profile, here was found with one more taxon than on the 1st profile. With increasing organic load, diversity decreased on the 2nd profile, although a more sensitive species was found here.

The purpose of this work was to describe the current status of benthic communities on the main stream of Elbe River and Ohře River and their tributaries affected and non-affected by biological invasions - especially by round goby (Neogobius melanostomus) and spiny - cheek crayfish (Faxonius limosus). Sampling for this research took place from April 2018 to August 2018, while morphiologic survey of waterbodies was done in November 2017. Two tributaries of the Elbe River (Milešovský brook and Ploučnice River) and one tributary of the Ohře River (Čepel) were selected for this research. Two 100 m long sections (lower stretch potentially affected by invaders and upper stretch as referential) were monitored at each tributary. At the same time a 100 m long main flow section was monitored near the mouth of the tributaries. The following parameters were monitored: morphology of the stream and basic physical and chemical parameters of water, zoobenthos community, crayfish occurrence, and fish community. The benthic communities of the upper streams were less affected by biological invasions. The biodiversity of the benthic communities in the upper sections was richer compared to the lower sections. The occurrence of the round goby seemed to be factor affecting benthic and fish communities at the sites concerned. It was an eudominant species in the Elbe section in Děčín and becoming dominant in the lower section of Ploučnice River.