Orientador:Caris Maroni Nunes / Co-orientador:David Anthony Orin Courtenay / Banca:Márcia Dalastra Laurenti / Banca:Mary Marcondes / Resumo:As estratégias de controle da leishmaniose visceral (LV) no Brasil estão baseadas no diagnóstico e tratamento precoce de casos humanos, no controle dos vetores, através do uso de inseticidas e na triagem sorológica com posterior eutanásia de cães positivos para leishmaniose. Assim, o diagnóstico da leishmaniose visceral canina (LVC) deve ser capaz de identificar adequadamente o reservatório canino, garantindo a eficiência desta medida de controle. Considerando-se que a resposta imune humoral desenvolvida por cães à infecção natural por Leishmania spp. é dependente da carga parasitária, o objetivo desta pesquisa foi avaliar se o resultado dos testes diagnósticos atualmente utilizados em inquéritos soro epidemiológicos no Brasil (teste rápido-DPP BioManguinhos® e teste ELISA indireto EIE-ELISA BioManguinhos®) apresentam relação com a carga parasitária do sangue de cães. Para tal foram avaliadas amostras de 610 cães da região noroeste do Estado de São Paulo a qual é endêmica para LV. A positividade variou de 15,9 a 68.4% acordo com as técnicas utilizadas, devido às diferenças na sensibilidade e especificidade dos testes. De modo geral, cães sorologicamente positivos apresentaram carga parasitária média maior que a dos cães negativos. Entretanto, a avaliação do potencial de transmissão dos cães sorologicamente negativos faz-se necessária / Abstract:Visceral leishmaniasis (VL) control strategies in Brazil are based on the early detection and treatment of the patients, vector control by insecticide and the serological screening and euthanasia of positive dogs. Thus, canine visceral leishmaniasis (CVL) diagnosis must accurately identify canine reservoir hosts to ensure the efficiency of screening. Considering that the humoral immune response of dogs to natural Leishmania spp. infection is dependent on parasite load, our aim was to evaluate if outcomes of the diagnostic kits currently used in canine seroepidemiological surveys in Brazil (DPP-BioManguinhos™ rapid test and EIA-ELISA Indirect ELISA BioManguinhos™) could be related to the blood parasite loads in dogs. Six hundred and 10 dogs from the VL endemic region of Northwest São Paulo State were sampled for this evaluation. Positivity varied from 15.9 to 68.4% between diagnostic techniques, due to differences in test sensitivity and specificity. Overall, positive dogs had higher mean parasite loads than negative dogs. Nevertheless, the potential of transmission of the negative dogs shall be investigated / Mestre

Made available in DSpace on 2015-10-06T13:02:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-17. Added 1 bitstream(s) on 2015-10-06T13:19:32Z : No. of bitstreams: 1 000849010.pdf: 646917 bytes, checksum: 6ff31432c39a9bdc92b648de138b7639 (MD5) / Visceral leishmaniasis (VL) control strategies in Brazil are based on the early detection and treatment of the patients, vector control by insecticide and the serological screening and euthanasia of positive dogs. Thus, canine visceral leishmaniasis (CVL) diagnosis must accurately identify canine reservoir hosts to ensure the efficiency of screening. Considering that the humoral immune response of dogs to natural Leishmania spp. infection is dependent on parasite load, our aim was to evaluate if outcomes of the diagnostic kits currently used in canine seroepidemiological surveys in Brazil (DPP-BioManguinhos™ rapid test and EIA-ELISA Indirect ELISA BioManguinhos™) could be related to the blood parasite loads in dogs. Six hundred and 10 dogs from the VL endemic region of Northwest São Paulo State were sampled for this evaluation. Positivity varied from 15.9 to 68.4% between diagnostic techniques, due to differences in test sensitivity and specificity. Overall, positive dogs had higher mean parasite loads than negative dogs. Nevertheless, the potential of transmission of the negative dogs shall be investigated