Hydraulic conductivity (K) is a key parameter required for the accurate prediction of contaminant transport in an aquifer. Traditionally, pump tests, slug tests, grain size analysis and, to a lesser extent, tracer tests have been employed to estimate the K of an aquifer. These methods have disadvantages in respect to assessing the K of a contaminated aquifer, for example, pumping tests generate large quantities of potentially contaminated water, slug tests interrogate only a small portion of aquifer to generate K values, and tracer tests are costly to perform. The recirculating well pair (RWP) system, assessed in this study, attempts to minimise these disadvantages while producing accurate estimates of K. The RWP system uses two wells, each screened in two positions; one screen injects water and the other extracts water from the aquifer. One well extracts water from the lower screen and injects it into the aquifer via the upper screen, whereas the second well extracts water from the upper screen and injects it through the lower screen. When these two wells are pumped in tandem a recirculation system is created within the aquifer. No water is lost or gained from the aquifer in this system. Hydraulic conductivity can be estimated from a RWP system by either the multi dipole or the fractional flow methods. The multi dipole method estimates K by measuring steady state hydraulic heads, whereas the fractional flow method uses a tracer test to obtain steady state concentrations at the four screens to estimate K. Both methods utilise a 3D flow model to simulate the aquifer system. Inverse modelling in conjunction with a genetic algorithm simulate the hydraulic head values obtained from the multi dipole experiments or the tracer steady state values obtained from the fractional flow method. Hydraulic ii conductivity estimates are obtained by matching the simulated and observed steady state hydraulic head, or tracer steady state values. An investigation of the accuracy of the two RWP methods, when system parameters are varied, in estimating K values was undertaken. Five multi dipole experiments were undertaken with varying dipole flow rates to assess the effect of altering dipole flow rate on estimates of K. Two experiments were also undertaken to assess the effect of altering the pumping well incidence angle as compared to the regional flow on the accuracy of K estimates. Five fractional flow experiments were conducted, four to assess the effect of changing dipole pumping rates and one to assess the influence of altering the incidence angle of the pumping wells on estimation of K. All experiments were undertaken in an artificial aquifer that allowed control of hydraulic parameters and accurate measurement of aquifer K by independent methods. Experimental results were modelled with the two RWP methods. Results indicate that both the multi dipole and fractional flow methods provide accurate estimates of the K of the artificial aquifer (5 % to 57% greater than the actual K and -14% to 17% of the actual K, respectively). Altering the ratio between the pumping well and regional aquifer flow rates had no effect on the estimated K results in both methods. Although preliminary results were positive, further work needs to be undertaken to determine if changing the orientation of the well pairs affects the estimation of K.

<p> Recirculating Sand Filters (RSFs) provide a compact method of secondary treatment to septic systems and lagoons, are relatively easy to operate and require little maintenance. Together, these characteristics render RSFs particularly appropriate for small communities and municipalities, as they offer a number of economic and operational advantages over conventional technologies. A preliminary study investigating RSF effluent quality, conducted jointly by McMaster University, the Great Lakes Sustainability Fund (GLSF) and the Ontario Ministry of the Environment (MOE) in 1999-2001, conducted pilot-scale experiments and demonstrated that municipal sewage can be successfully treated year-round by RSFs. The results of the preliminary study recommended that further work be conducted to investigate the selection of media size, dosing frequency, recycle ratio, and hydraulic loading rate. </p> <p> The primary objective of this study was to develop design and operating conditions under Ontario climatic conditions with respect to media size, dosing frequency, recycle ratio and hydraulic loading rate by conducting further pilot-scale studies. Three pilot-scale RSFs, operating in parallel, were loaded intermittently with septic tank effluent to evaluate the above mentioned operating parameters on the removal of total suspended solids (TSS), 5-day carbonaceous biochemical oxygen demand (cBOD5), total ammonia-nitrogen (TAN) and total nitrogen (TN). The addition of alum was also implemented to evaluate the removal of total phosphorus (TP). The effluent objectives for this study were based on the MOE general secondary treatment level requirements of monthly averages based on a minimum of four weekly samples. The four-phase experimental program began in April, 2004 and ended in June, 2005. Three media sizes were investigated, with d10 of 2.6, 5 and 7.7 ·mm. The applied hydraulic loading rates were 0.2 and 0.4 rnlday. Dosing frequencies of 24 and 48 times/day were observed. Recycle ratios of 300% and 500% were also evaluated. </p> <p> It was found that the RSF operating with 2.6 mm media, 500% recycle ratio and 24 times/day dosing frequency under a hydraulic loading rate of 0.2 rnlday produced the best quality effluent, and achieved the effluent objectives required by the MOE. These operating criteria, however, must still be investigated under cold weather conditions to ensure acceptable year-round performance in Ontario. With proper addition of alum, the TP effluent objective was achieved under the optimum operating conditions. </p> / Thesis / Master of Applied Science (MASc)

Despite being a low fat fish and consequently a low omega-3 fish, tilapia have widespread consumer acceptability due to its mild taste, cheap price and low mercury content. However some sources claim that farmed tilapia can be detrimental to human health due to high omega-6:3 ratios and low omega-3 content specifically eicosapentaenoic acid, docosapentaenoic acid, and docosahexaenoic acid. The objective of this study was to create an omega-3 enriching feed that would increase omega-3 content in tilapia and subsequently decrease the omega-6:3 ratio. An 8 week feeding trial was conducted. Tilapia were cultured in a recirculating aquaculture system on one of eight diets (control, commercial, 1, 3, 5% fish oil or 1.75, 5.26, 8.77% ALL-G-Rich (algae). Water quality, selected fish biometrics and growth performance were recorded. Fillet and rib meat tissues were collected at weeks 4 and 8, and liver and mesenteric fat tissues were collected at week 8. Fat was extracted, trans-methylated and identified as fatty acid methyl esters using gas chromatography-mass spectrometry. Docosahexaenoic acid increased in concentration in all tissues as percent fish oil and ALL-G-Rich increased in the diets with 8.77% ALL-G-Rich resulting in significantly (P<0.0001) greater concentrations in the fillet and mesenteric fat compared to all other diets after 8 weeks. The 8.77% ALL-G-Rich diet resulted in significantly (P=0.003) greater cumulative accumulation of EPA, DPA and DHA on a mg/4oz fillet basis after 4 weeks compared to control. The results of this study suggest that an ALL-G-Rich finishing feed could be produced that would result in a value added farmed tilapia fillet. / Master of Science in Life Sciences

Stepped chutes have been used as hydraulic structures for more than 3.5 millennia for different purposes: For example, to dissipate energy, to enhance aeration rate in the flow and to comply with aesthetical functions. They can be found acting as spillways in dams and weirs, as energy dissipators in artificial channels, gutters and rivers, and as aeration enhancers in water treatment plants and fountains. Spillways are used to prevent dam overtopping caused by floodwaters. Their design has changed through the centuries. In ancient times, some civilizations used steps to dissipate energy in open channels and dam over-falls in a similar fashion as natural cascades. However, in the first half of the twentieth century, the use of concrete became popular and the hydraulic jump was introduced as an efficient energy dissipator. In turn, the use of a stepped geometry became obsolete and was replaced with smooth chutes followed by hydraulic jump stilling basins. In recent years, new construction techniques and materials (Roller Compacted Concrete RCC, rip-rap gabions, wire-meshed gabions, etc.) together with the development of new applications (e.g. re-aeration cascades, fish ladders and embankment overtopping protection or secondary spillways) have allowed cheaper construction of stepped chutes, increasing the interest in stepped chute design. During the last three decades, research in the hydraulics of stepped spillways has been very active. However, studies prior to 1993 neglected the effect of free-surface aeration. A number of studies since this time have focused on air-water flows in steep chutes (θ ≈ 50o). But experimental data is still scarce, and the hydraulic performance of stepped cascades with moderate slope is not yet understood. This study details an experimental investigation of physical air-water flow characteristics down a stepped spillway conducted in two laboratory models with moderate slopes: the first model was a 3.15 m long stepped chute with a 15.9o slope comprising two interchangeable-height steps (h = 0.1 m and h = 0.05 m); the second model was a 2.5 m long, stepped channel with a 21.8o slope comprising 10 steps (h = 0.1 m). Different arrangements of turbulence manipulators (vanes) were also placed throughout the chute in the second model. A broad range of discharges within transition and skimming flow regimes was investigated to obtain a reliable representation of the air-water flow properties. Measurements were conducted using single and double tip conductivity probes at multiple span wise locations and at streamwise distances along the cavity between step edges to obtain a complete three-dimensional representation of the flow. Although the present study was conducted for two moderate slope chutes (θ = 15.9º & 21.8o), it is believed that the outcomes are valid for a wider range of chute geometry and flow conditions. The purpose of this study is to improve the understanding of turbulent air-water flows cascading down moderate slope stepped chutes, and gain new understandings of the interactions between aeration rate, flow turbulence and energy dissipation; scale effects are also investigated. The study provides new, original insights into air-water turbulent flows cascading down moderate slope stepped spillways not foreseen in prior studies, thus contributing to improve criterion designs. It also presents an extensive experimental database (available in a CD-ROM attached at the end of this thesis) and a new design criterion that can be used by designers and researchers to improve the operation of stepped chutes with moderate slopes. The present thesis work included a twofold approach. Firstly, the study provided a detailed investigation of the energy dissipative properties of a stepped channel, based upon detailed airwater flow characteristics measurements conducted with sub-millimetric conductivity probes. Secondly, the study focused on the microscopic scale properties of the airwater flow, using the experimental data to quantify the microscopic scale physical processes (e.g. momentum transfer, shear layer development, vertical mixing, airbubbles/ water-droplets break-up and coalescence etc.) that are believed to increase the flow resistance in stepped canals. The study highlighted the tridimensionality of skimming flows and hinted new means of enhancing flow resistance by manipulating turbulence in the stepped chute. Basic dimensional analysis results emphasized that physical modelling of stepped chutes is more sensitive to scale effects than classical smooth-invert chute studies and thus suggested that the extrapolation of results obtained from heavily scaled experimental models should be avoided. The present study also demonstrated that alterations of flow recirculation and fluid exchanges between free-stream and cavity flow affects drastically form losses and in turn the rate of energy dissipation. The introduction of vanes demonstrated simple turbulence manipulation and form drag modification that could lead to more efficient designs in terms of energy rate dissipation without significant structural load on the stepped chute.

Thesis--Auburn University, 2009. / Abstract. Includes bibliographical references (p. 40-43).

Recirculating turbulent flow within a cavity with an inlet wall jet was examined. In steady water flow velocity profiles were constructed with measurements taken with a Laser Doppler Anemometer system mounted on a traversing mechanism. Two test cases were examined Re jet = 1167 and Re jet = 3231 as well as developing wall jet profiles. The results are presented with mean velocity plots and turbulent kinetic energy contours. Comparisons are then made with results obtained using a finite difference computational scheme based on the k - e turbulence model. Good agreement was obtained between the computer code predictions and the experimental data. / Thesis / Master of Engineering (ME)

With World Health Organization estimation of population increase to over 9 billion by 2050, malnutrition and food insecurity are predicated to be two of the main challenges facing the world in the future. Fish is a source of protein that can have a significant contribution for solving the global food crisis. Fish for human consumption is provided by both the aquaculture industry and the capture fisheries, however, the flat-lined production of seafood from capture fisheries means aquaculture production should double in number to provide enough protein by 2050. Culturing fish at higher densities is being used as a way to increase the global aquaculture production. Modern aquaculture intensified practices expose the fish to various stressors that can negatively impact their welfare. Good nutrition can improve the health status of aquacultured fish while accumulation of specific nutrient can add value to the fillet. In line with that, the studies described in this dissertation aimed to examine the vital role of fish in human health and evaluate strategies to improve the sustainable aquaculture production of fish using novel aquafeeds. This was completed by conducting a survey of the essential minerals and heavy metals of commercially available catfish, salmon, and tilapia fillets in the United States marketplace and three fish nutritional trials to evaluate the impacts of novel aquafeeds on production and health of fish and the food quality of their respective fillets. Selenium with the established health benefit for human and fish was the trace mineral of interest in this dissertation. This element is the main component of a group of selenium containing proteins called selenoproteins that are antioxidant and maintain oxidative homeostasis of fish and human. Additionally, selenium is an antagonist for many heavy metals such as mercury. The market study revealed that catfish, salmon and tilapia samples available in United States marketplace are good source of selenium, zinc and phosphorous. The concentration of heavy metals in all the samples were lower than safety standards. Nile tilapia (Oreochromis niloticus) and rainbow trout (Oncorhynchus mykiss), which are two economically important aquacultured fish, were used as animal models in the fish nutritional trials. Different forms of selenium supplemented to Nile tilapia diet. The form of Dietary Se form did not have any significant (p>0.05) effect on growth, and plasma and hepatic GPX activity of Nile tilapia. However, organic selenium supplement showed significantly (p<0.05) higher bioavailability and accumulated in the fillets at the significantly higher concentration. In the second fish trial selenoneine, a novel selenium containing compound, was supplemented at various concentrations to rainbow trout diet. Other than growth parameters multiple other health biomarkers (lysozyme, glutathione peroxidase, superoxide dismutase activities) were examined to determine whether selenoneine supplementation had a positive impact on fish health. Selenoneine enriched diet significantly(p<0.05) increased the weight gain, lysozyme and oxidative enzymes activities of rainbow trout. Lastly rainbow trout feed was supplemented with commercially available probiotic (Actisaf®), prebiotic (Safmmann®), and their mixture (synbiotic). These dietary supplements did not have any significant effect on fish production but led to a significant increase in activity of oxidative enzymes in plasma when compared to control diet. These results suggest that feed supplements used in this dissertation can be used to promote the fish health raised in recirculating aquaculture systems and support an economically and environmentally sustainable aquaculture. / PHD / Fish contributes significantly to human’s diet in terms of high quality, easily digestible animal protein that helps fight malnutrition and food insecurity. More than half of the fish for human consumption is produced by the aquaculture industry and the growth of the industry is very critical for meeting the future needs for protein. Other than production of high-quality protein the aquaculture industry has made a great contribution to people’s livelihood, poverty alleviation, income generation, employment and, trade globally. To continue contributing effectively to global food security aquaculture industry should overcome various obstacles such as disease and sustainable production of high-quality feed. In this dissertation, important roles of fish in human diet was examined by measuring the mineral composition of commercially available catfish, salmon and tilapia in the United States market place. Also diets of Nile tilapia and rainbow trout were supplemented with various forms of selenium, prebiotics, and/or probiotics which have positive effect on health of both human and fish. These supplementations led to production of valued-added fish fillets enriched in selenium and improved the fish growth and health.

Accumulations of fine sediments in deep-ocean contourites form a sedimentary record that has been hypothesized to be directly related to bottom-current behavior. This is known as the 'sortable silt' hypothesis and states that the non-cohesive, coarse silt in the 10 to 63 µm size range within a deposit can be used as a proxy for paleocurrent velocity. Slow deposition rates on contourites (2−10 cm/kyr) make it difficult to test this hypothesis in the field and few laboratory studies have been conducted. To test the 'sortable silt' hypothesis in the laboratory, a non-recirculating flume was constructed in which silt and clay could be deposited under a variety of velocities, sediment concentrations, and silt to clay ratios. Samples of the deposited material from each experiment were analyzed to determine the grain-size distribution using a Micromeritics Sedigraph 5120 particle size analyzer. The results of these experiments were used to evaluate the following two hypotheses: 1. The proportion of sortable silt (SS%) compared to the proportion of clay is a better indicator of current velocity than the mean size of the sortable silt (SS). 2. The presence of clay will impact the movement and sorting of silt in the bed. Results show that increased velocity correlates with increased (SS), and that (SS) generally decreases downstream of the sediment source. (SS) was found to be more representative of velocity than (SS%) and, counter to the original hypothesis, clay did not have a significant effect on silt deposition. / Master of Science / The ’sortable silt’ hypothesis states that there is a relationship between the velocity of an ocean current and the size of the sediment that deposits on the bottom of the ocean. These deep-ocean deposits consist of material smaller than sand such as clay and silt. Smaller particles require less force than larger particles to remain suspended, and higher current velocities produce larger forces. For this reason larger current velocities are thought to be associated with the deposition of coarser sediments. It is challenging to test this hypothesis in the field because of the cost and the slow rates at which change occurs. Laboratory studies can help to overcome these challenged by test scenarios otherwise impossible in the field. For this research, a flume was constructed and used to examine how different sediment types sort under flowing water. Most laboratory flumes recirculate water using pumps, but this flume does not. A mixture of dry material and water flows through the flume, depositing a bed over time. This deposited material can then be tested for its size parameters. These size parameters are compared to the material type and the velocity of the current in the flume to help answer two main questions: 1. Is the amount of silt in a sample a better indication of the current velocity than the average size of the material deposited? 2. Will the addition of clay will change the way silt deposits in the system?. Results show that silt does sort with increasing velocity and that the mean sortable silt size is good indicator of current velocity.

The purpose of this study is to modify an existing static analytical model developed for a Re-circulating Aquaculture Systems through incorporation of risk considerations to evaluate the economic viability of the system. In addition the objective of this analysis is to provide a well documented risk based analytical system so that individuals (investors/lenders) can use it to tailor the analysis to their own investment decisions—that is to collect the input data, run the model, and interpret the results. The Aquaculture Economic Cost Model (AECM) was developed by Dr. Charles Coale, Jr. and others from the department of Agricultural and Applied Economics at Virginia Tech. The AECM is a spreadsheet model that was developed to help re-circulating aquaculture producers make strategic business decisions. The model can be used by potential producers interested in investing in re-circulating aquaculture through development of a financial analysis that in turn will help them obtain funding for the enterprise. The model is also useful for current producers who want to isolate inefficient aspects of their operation. AECM model consists of three major sections which include the Data Entry, Calculations and Analysis. The first section requires that the producer conducts background research about their operation to ensure accurate calculation and analysis. The calculation section provides a great deal of information about the operation's finances, while the analysis section provides information about the operation's financial stability. While the AECM is a powerful model, it is based on single, usually mean, values for prices, costs, and input and output quantities. However, market, financial and production uncertainties result in fluctuating prices, costs and yields. An individual who is making management decisions for a re-circulating aquaculture system will be faced with some or all of these uncertainties. By adding simulation to the AECM model to account for these uncertainties individuals will be able to make better management decisions. Information of the varying likelihoods or probabilities of achieving profits will be of crucial interest to individuals who plan on entering into or modifying an existing aquaculture system. Risks associated with six variables were examined in this paper: feed cost, feed conversion, mortality rate, capital interest rate, final weight, and output price. Data for the Interest Rate and output price were obtained from the Federal Reserve System and NMFS website respectively. Expert opinion was the source of data for the other variables. After probability distributions were applied to the random variables to account for the uncertainty the model was simulated for ten thousand iterations to obtain expected returns for three years in advance that the model calculates an income statement. In addition to that, sensitivity analyses were carried out in order to inform the producer which factors are contributing the most to the profitability of the operation. In this way the producer will have a better idea as to which aspects of the operation to monitor closely and consider modifying. The analysis shows that the mean income for the three years will be negative and thus the business would be losing money. The simulated mean net incomes were: -$216,905, -$53,689, -$53,111 for year1 through year3 respectively. Sensitivity analysis confirmed that output price is by far the most significant input that makes the overall bottom line to fluctuate most. Output price was on top of the list for all the three years analyzed in this study. Feed cost and Feed conversion were the next most significant inputs. The other inputs were also significant in explaining the fluctuation of the bottom line; however both their regression and correlation coefficients were small. / Master of Science

In order to facilitate research and testing of kV/MVA class power electronics systems, Virginia Tech has constructed the High-Power lab facility. This lab supports testing of equipment operating at up to 1.3 MW, with maximum supply ratings of 4,160 V or 480 A, depending on how the system is configured. When operated as a recirculating power ring, the system will make minimal demands on utilities. An industrial supervisory, control, and data acquisition (SCADA) system will be used to control the facility. In this paper we will detail the lab design and give insight to the decisions behind it, with an aim toward helping the reader in their own similar effort. / Master of Science