In this thesis we are focusing at the notion of psychical distance first introduced in the work "'Psychical Distance' as a Factor in Art and as an Aesthetic Principle" from Edward Bullough. There were many responses to the concept of psychical distance from many authors, some of them have rejected this notion, and others tried to support it. Initially we provided brief interpretation of Bulough's work with the focus at parts, which are cited and investigated most frequently in mentioned interpretations. We are interpreting and comparing gradually the most popular works, which involve the notion of psychical distance with respect to Bullough's original concept. We are pointing out the difference between notions psychical distance and aesthetic attitude, because they are mistaken one for another by many different authors. This thesis tries to provide new look at some papers responding to Bullough's conception.

O presente trabalho teve, como objetivos, a) desenvolver um procedimento para estabelecer, em abelhas Melipona quadrifasciata anthidioides, a resposta de escolha de acordo com o modelo, com base em dados da literatura; b) testar o procedimento elaborado; c) aperfeiçoar ou modificar esse procedimento, com base nos dados obtidos. Como o procedimento, inicialmente proposto para ser testado, tivesse se mostrado ineficaz para instalar a resposta desejada, várias modificações foram efetuadas com base nos dados que estavam sendo produzidos, até chegar à elaboração de um procedimento eficaz para instalar, nos sujeitos, a resposta de escolha de acordo com o modelo, utilizando estímulos de duas cores diferentes. Esse procedimento iniciava-se com a apresentação de apenas dois cartões de tamanhos diferentes, sendo que o maior (SD ) englobava os estímulos modelo e de comparação de mesma cor. Através de fading da dimensão tamanho, o modelo era gradualmente separado do estímulo de comparação de mesma cor, ate que os três estímulos ficassem igualmente espaçados e distribuídos em três cartões de igual tamanho. Os resultados obtidos com esse procedimento mostraram um ,aumento gradativo na porcentagem de RD, até estabilizar na porcentagem máxima; cada aumento na distância entre os estímulos era seguido de uma diminuição, pequena e de curta duração, na porcentagem de RD Esses resultados, e os obtidos com outros procedimentos utilizados, foram discutidos em ter mos dos efeitos das modificações efetuadas na situação experimental sobre o desempenho dos sujeitos. / The present work has, as main goal, the development of a procedure to stablish matching-to-sample response in bees (Melipona quadrifasciata anthidioides ). Based on an analysis of the literature, a procedure was chosed. The data of the testing of such a procedure, showed the need to introduce several modifications, until gradual improvement of the final procedure was achieved. The dimension along which the work was developed was wavelength. On the final procedure, two post cards of different sizes were used, the e larger one contained both the standard and one of the comparison stimuli, the smaller card contained the remaining comparison stimulus, and initially this was always the one not-to-be-chosen. Gradually, standard and comparison stimuli present in the same card were separated, by fading the size dimension, until all three stimuli were evenly spaced and distributed on three equal size cards. Under this procedure, correct responses showed a gradual increase reaching the maximum percentage of correct responses. Each increase in the space between the standard and the to-be-chosen comparison stimulus was followed by an small and short living decrease in the percentage of correct responses. Different features of this, and other discarded procedures were analyzed in connection to the subjects performance.

Nesta dissertação, o objetivo é investigar um conjunto de redações do Exame Nacional do Ensino Médio (Enem)/2012, analisando as relações dialógicas estabelecidas pelos escreventes a partir da interação ativa com as vozes reportadas para a defesa de um ponto de vista sobre o tema: O movimento imigratório para o Brasil no século XXI. Desde 2009, o Enem seleciona candidatos para o ingresso no ensino superior e a redação é o instrumento que solicita a elaboração de um texto dissertativo-argumentativo. A partir do total de 2720 redações cedidas pelo Instituto Nacional de Estudos e Pesquisas Educacionais Anísio Teixeira (Inep), o corpus foi constituído por 121 redações, segundo dois critérios: (a) a faixa de desempenho de 200 a 1000 pontos, respeitando a diversidade de notas, e (b) as cinco regiões brasileiras, marcando a representatividade regional. A fundamentação teórica deste trabalho centra-se na perspectiva dialógica da linguagem de Bakhtin e o Círculo, principalmente, nos conceitos de enunciado concreto e discurso citado, e na perspectiva ideológica dos estudos de letramento. Assumindo o trabalho com a escrita como um processo de compreensão responsiva, esta pesquisa buscou compreender cada texto como uma réplica ativa à proposta de redação e aos discursos oficiais que dela ecoam. No conjunto das redações, foram identificados quatro tipos de réplicas, que serviram como eixos norteadores para a análise dos modos heterogêneos de como os escreventes responderam às instruções objetivas da proposta e ao tema da imigração: (i) réplicas à exigência dissertativa; (ii) réplicas aos textos da coletânea, (iii) réplicas à imagem da identidade nacional e (iv) réplicas à história oficial do Brasil. Dentro de cada uma dessas réplicas, os resultados mostraram que os escreventes utilizaram procedimentos linguístico-discursivos para a construção do texto argumentativo, tais como: citações nos diferentes estágios composicionais da dissertação, paráfrases associadas à síntese, cópia e imitação dos textos da coletânea, construções referenciais para a caracterização do Brasil, como o uso de slogans, a mobilização de alusões históricas e narrativas da colonização. Tais procedimentos foram usados para assumir posicionamentos polêmicos e não polêmicos, alguns empregados com tendência à reprodução da palavra alheia e outros com vistas a sua reelaboração. Sob um olhar dialógico-axiológico, a análise da apreensão dos discursos de dentro e de fora da coletânea constatou não só o engendramento ativo dos sujeitos na produção de sentidos, mas os seus direcionamentos para os interlocutores presumidos e para as vozes institucionais dentro da rede de relações que envolve o projeto de escrita na avaliação do Enem. As réplicas puderam mostrar percursos discursivos construídos no diálogo com diferentes repertórios sociais, culturais e linguísticos, sinalizando que o trabalho com a escrita não está desvinculado dos contextos sócio-históricos das práticas letradas dos escreventes, refletindo e refratando os modelos normativos. / In this dissertation, the goal is to investigate a collection of essays from the National Secondary Brazilian Examination (Enem)/2012, analyzing the dialogic relations established by the writers from the active interaction with the reported voices to defend a point of view on the topic \"the immigration movement to Brazil in the 21st century\". Since 2009, Enem selects candidates for admission to higher education and the writing is the instrument that calls for the development of a dissertative-argumentative text. From the total of 2720 essays provided by the National Institute for Educational Studies Anísio Teixeira (INEP), the corpus was consisted of 121 essays, according to two criteria of selection: a) the performance range of 200-1000 points, respecting the diversity of scores, and b) the five Brazilian regions, marking the regional representation. The theoretical foundation of this work focuses on the dialogic language perspective of Bakhtin and the Circle, mainly, the concepts of \"utterance\" and \"quoted speech,\" and the ideological perspective of the literacy studies. Assuming the job with writing as a process of responsive understanding, this research sought to understand each text as an active response to the writing proposal and to the official discourses. In all the texts, four types of responses were identified, which served as a guide for the analysis of the heterogeneous modes of how the participants answered to the instructions of the proposal and to the issue of immigration: (i) responses to the dissertation requirement; (ii) responses to the texts of the collection, (iii) responses to the image of national identity and (iv) responses to the official history of Brazil. Within each of these responses, the results showed that the subjects used linguistic-discursive procedures for the construction of the argumentative text, such as: quotes in different compositional stages of the dissertation, paraphrases associated with synthesis, copy and imitation of the texts from the collection, characterization of Brazil as the use of slogans, the mobilization of historical allusions and narrative of colonization. These procedures were used to take controversial positions and not controversial ones, some employed with the tendency to reproduce the alien word and others with the tendency to remake them. Under a dialogical axiological perspective, the analysis of the comprehension of the inside and outside discourses of the collection found not only the active gendering of the subjects in the production of meanings, but their directions for the presumed actors and to the institutional voices within the network relationships of the writing project on the assessment of Enem. The responses could show discursive routes constructed in dialogue with different social, cultural and linguistic repertoires, signaling that the work with writing is not disconnected from the socio historical contexts of the student´s literate practices, reflecting and refracting the normative models.

This thesis describes the development of the real-time convolution system (RTCS) for a little-studied talker/listener in virtual acoustic environments. We include descriptions of the high-resolution directivity measurements of human speech, the RTCS system components, the measurement and characterization of oral-binaural room impulse responses (OBRIRs) for a variety of acoustic environments, and the compensation filter necessary for its validity. In addition to incorporating the high-resolution directivity measurements, this RTCS improved on that developed by Cabrera et al. [1] through the derivation and inclusion of the compensation filter. Objective measures in the time- and frequency-domains, as well as subjective measures, were developed to asses the validity of the RTCS. The utility of the RTCS is demonstrated in the study on vocal effort, and the results of an initial investigation into the vocal effort data are presented.

As early career researchers, we all want to be successful in securing our first big grant and publishing our research findings in the most prestigious journals. To achieve this success, we need to be able to understand why our grants proposals weren't funded in a grant cycle and why we are asked to make major revisions to the manuscript that we spent so much time on already. Even worse is when we get an outright rejection and don't understand why. A very important skill as a researcher is to be able to interpret the comments from grant and manuscript reviews objectively once we recover from the initial reaction of sadness, frustration, or even anger. This session will provide strategies to tackle the reviewers' comments that will hopefully lead to a successful resubmission.

Labeled by the National Academy of Engineering (NAE) as one of fourteen Grand Challenges for Engineering, the management of the nitrogen cycle has become an increasingly difficult obstacle for sustainable development. In an effort to help overcome this challenge, the goal of our study is to expand on the limited scientific understanding of how the nitrogen cycle within aquatic environments may be affected by increasing human- and climate-induced changes. To this end, we are using freshwater mussels as a sentinel species to better understand the impacts of ecosystem perturbation on nitrogen processing in large river systems. This was completed by examining the physical, biological, and chemical characteristics of a mussel habitat in the Mississippi River, evaluating the impact of the 2008 floods on the habitat and the ecosystem's nutrient processing, establishing a well-equipped mussel laboratory habitat to investigate mussel behavioral responses, and analyzing highly time resolved data to examine the mussels' contribution to daily nitrate fluxes.

Immunologic competence is correlated with resistance to infectious disease which may be affected by exposure to certain compounds. Methyl parathion (MP) and carbofuran (CF) have been reported to affect the development of active immunity in the rabbit and the present research was conducted to quantify the dosage relationships of these two pesticides to both host resistance and acquired resistance in the mouse. Preliminary study was made on the virulence of Salmonella typhimurium in mice. Mortality rates among infected animals fed Purina Laboratory Chow diet providing 0.08, 0.7 3.0 mg MP/kg/day or 0.1, 0.6, 1.0 mg CF/kg/day along with untreated controls were determined and protection afforded the animals by vaccination under the influence of chemical treatment was examined. The nature of dose response was further investigated with the study of various parameters of host defense against microbial infection. Measurements were made of the bacterial population in tissues of mice during and after the course of infection, differential leukocyte counts, in vivo and in vitro opsonic effect of serum, clearance of the reticulo-endothelial system, agglutination titers of serum, total serum gamma-globulin levels, serum immunoglobulin levels with classes and subclasses, and lymphocyte response to mitogen stimulation. Dosage-related increases in mortality were seen in unvaccinated mice under both chemical treatments, and protection by immunization was decreased. Pesticide treatment extending beyond two weeks was required to obtain significant increases in mortality. Increased mortality was associated with increased numbers of viable bacteria in blood, decreased total gamma-globulins and specific immunoglobulins in serum, and reduced splenic blast transformation in response to mitogens. Serum opsonic activity of CF-treated animals was slightly reduced. These results support those of other investigations indicating effects of environmental toxicants upon the resistance and immune competency of experimental animals.

The cognitive and affective characteristics and responses to stimulant medication of children who were diagnosed as having attention deficit-hyperactivity disorder (AD-HD) or undifferentiated-attention deficit disorder (UADD) were investigated using a pretest-posttest experimental design. Nineteen AD-HD and 17 UADD children were compared using unpaired t-tests, prior to initiation of stimulant medication, on measures of intellectual functioning, impulsivity, problem behavior, and self-reported depression and self-esteem. Children from both the AD-HD (n = 12) and UADD (n = 12) groups were then compared before and after a 3-month trial of stimulant medication on measures of impulsivity, problem behavior, and self-reported depression and self-esteem using repeated measures analyses of variance. No significant differences were found between groups in cognitive ability, impulsivity, depression, self-esteem, anxiety, peer relationships, or social withdrawal. AD-HD children were found to exhibit more hyperactive, aggressive, and delinquent problem behavior. Significant improvement was found in both groups in self-reported depression and self-esteem following a trial of stimulant medication. A trial of stimulant medication was found to reduce hyperactive problem behavior in AD-HD children. Stimulant medication had a beneficial effect on peer relationships and aggressive problem behavior in UADD children but did not produce similar positive effects in AD-HD children. These results are discussed as in relationship to the issue of whether AD-HD and UADD are separate syndromes and to the previous literature regarding attention deficit disorder with hyperactivity (ADD/H) and attention deficit disorder without hyperactivity (ADD/WO). Ramifications regarding appropriate treatment are also discussed.

Disparities in the use, quality, and outcomes of treatments, and the barriers that deter Hispanics from receiving healthcare services have been widely studied. Yet, similar efforts have been slow in the field of Applied Behavior Analysis (ABA). A way to decrease treatment disparities is to assess the influence of cultural variables in behavior analytic interventions, and to evaluate whether manipulations to these variables improve the overall results of the interventions among Hispanic families. During this study, we adapted functional communication training (FCT) to Hispanic parent’s cultural values, we assessed the preference for culturally adapted and non-culturally adapted functional communication responses (FCR), and we evaluated the social validity of both FCRs. We achieved this by creating the Culturally-Adapted Response Evaluation Survey (CARES) for behavior analysts to use as a guide when interviewing parents to identify an FCR that adheres to the families’ cultural values. We evaluated parents’ preference for each FCR using a multiple-baseline design in which parents were trained to implement procedures to maintain the FCR that was taught and we provided parents an opportunity to choose the FCR they wanted to reinforce. The results of the study and their implications for ABA services are discussed.

The western rock lobster (WRL), Panulirus cygnus is a decapod crustacean which is found in abundance in the coastal waters of Western Australia and which supports a major fishery of economic importance for the State, with an annual harvest ranging between 10-12 million kilograms. The growth of the existing markets in Asia for live exports and the competition exerted by other countries marketing spiny lobsters prompted the need for the Industry to assess and develop post-harvest handling procedures likely to contribute to an improved quality of live product. The physiological responses of P. cygnus to handling and transportation were virtually unexplored.The objectives of this project were, (i) to generate information with regard to the biological phenomena underlying morbidity and mortality of lobsters during live export shipments, (ii) to investigate the physiological responses of P. cygnus to the post-harvest handling practices currently used by the WRL industry in their five export program and, (iii) to identify protocols by which post-harvest handling techniques could be modified to reduce the occurrence of morbidity and mortality during five export.These objectives were achieved through, (i) a field survey conducted during the course of the 1992/93 fishing season, investigating the relative influence of environmental factors and processing techniques on the incidence of lobster morbidity+mortality during simulated live shipments and, (ii) a series of field experiments that monitored the physiological responses elicited by lobsters to post-harvest handling procedures and simulated live shipment conditions.From the field survey, it appeared that the rate of morbidity+mortality experienced in simulated live shipments, within the three processing units surveyed, averaged 5.22 +/- 0.63 %, with a highly significant difference (p [less than] 0.001) between the ++ / processing units. Scrutiny of the data revealed a major influence of two factors directly related to post-harvest handling procedures: a) the time spent under normal commercial conditions in packaging export cartons and, b) the ambient temperature within the export cartons. Following 30-36 hrs in packaging, the percent mortality increased twofold, from 5.2 +/- 0.0 % recorded following 20 - 24 hrs to 10.4 +/- 2.3%. With regard to the ambient temperature within the export carton, temperatures between 17.5 and 20.0 degrees celsius appear to be optimal for P. cygnus survival while temperatures above 20.0 degrees celsius induce significantly greater morbidity and mortality.A number of factors were identified as having a potential influence on lobster physiological responses to post-harvest handling conditions:a)Immediately after harvesting, lobsters are subjected to a range of transport environment options, according to their origins: i.e., (i) direct delivery from fishing vessels by local fishermen to the processing facility, (ii) onshore transportation by truck from coastal depots and, (iii) transport in baskets on board carrier-boats from the Houtman Abrolhos Islands.b) After delivery, lobsters are subjected to sorting and grading procedures, with a concomitant exposure to air and disturbance.c) Recovery in holding tanks is usually allowed for a period ranging between 24 to 72 hours.d) Prior to being packed in export cartons, lobsters are subjected to a short period (30 sec to 3 min) of immersion in chilled water (8 - 12 degrees celsius), the procedure varying from one processor to the other.e) During subsequent periods of transit in export cartons, (up to 48 hrs) lobsters are subjected to aerial exposure and fluctuating ambient temperature.A comparison of the physiological profiles of lobsters from different origins revealed significant differences, with respect to a ++ / range of physiological variables. On the basis of visual estimates of health status, lobsters from the "local" origin exhibited a consistently superior condition, when compared to "coastal" and "carrier-boat" animals. The examination of physiological variables revealed consistent trends reflecting the visual assessments. "Local" lobsters exhibited significantly lower levels in anaerobic metabolic waste concentrations, with the haemolymph lactate titre between 2.77 +/- 0.19 and 4.33 +/- 0.56 mmol L(subscript)-1, compared with the other groups, between 5.23 +/- 0.24 and 8.86 +/- 1.29 mmol L(subscript)-1. A 250 to 300% increase in haemolymph ammonia concentration was observed between "coastal" and "carrier-boat" groups and the "local" lobsters, at 0.32 +/- 0.02 mmol L(subscript)-1. Significantly higher pH values were recorded for the "local" group, at 7.72 +/- 0.04, compared with values below 7.64 +/- 0.04 for the other groups. "Local" lobsters recorded 8 to 15 times less circulating glucose, at 0.11 +/- 0.03 mmol L(subscript)-1, having 50% more ATP in their muscle tissues, at 6.07 +/- 0.15 mu mol g(subscript)-1 and 250% more arginine phosphate, at 6.56 +/- 0.72 mu mol g(subscript)-1.An assessment of the efficiency of the industry sorting procedures revealed differences between selected and rejected animals with regard to the ATP and arginine phosphate concentrations in their muscle tissues with, as a common trend for both variables, selected animals recorded higher values. Significant differences were identified within the "local" group of lobsters, with selected animals recording 40% more ATP, at 6.92 +/- 0.63 mu mol g(subscript)-1 and 30 % more arginine phosphate, at 7.77 +/- 1.01 mu mol g(subscript)-1.A monitoring of the physiological profiles of lobsters subjected to extended (up to 8 hours) periods of onshore transportation in trucks revealed a significant ++ / reduction in their health status. A consistent and almost linear fall in the concentration of total adenylate (35%), to 5.46 +/- 0.50 mu mol g(subscript)-1 and phosphagen reserve (70%),to 2.77 +/- 0.26 mu mol g(subscript)-1, were recorded throughout the 8 hour period. For up to 6 hours the concentration of lactate in the leg muscle tissues increased by 0.95 mu mol g(subscript)-1 h(subscript)-1 and then by 4.7 mu mol g(subscript)-1 h(subscript)-1,to reach 20.57 +/- 1.61 mu mol g(subscript)-1 after 8 hours. Haemolymph glucose and ammonia titres recorded a 3.5 fold increase over the first 6 hours, to reach 2.14 +/- 0.54 and 1.17 +/- 0.16 mmol L(subscript)-1, respectively, the last period (6 to 8 hours) being characterised by a 1.6 and 1.9 fold decrease in concentration. Lobsters were able to maintain their haemolymph pH close to 7.77 during the first four hours, with a concomitant rise in haemolymph calcium concentration. From the results, it appeared that, under current industry procedures, extending the period during which lobsters are transported in spray trucks to more than 6 hours is conducive of altered physiological status.An assessment of the effects of short periods (up to 60 min) of aerial exposure and disturbance revealed significant changes in the physiological profiles of lobsters. When exposed to air, lobsters exhibited a significant fall in haemolymph pH, a rise in lactate concentration, and a depletion in energy reserves. Lobsters left undisturbed were able to buffer an incipient acidosis for up to 40 min (7.78 +/- 0.03), after which a decline in pH was recorded to reach 7.71 +/- 0.02. Conversely, disturbed animals experienced an uncompensated acidosis and a decrease by 0.7 of a unit over a 60 minute period. Similarly, undisturbed lobsters did not demonstrate behavioural signs of stress while disturbed animals exhibited dramatically diminished responses ++ / to handling after 60 min of exposure. Both disturbed and undisturbed animals recorded a decline in ATP/ADP, to reach after 40 min, 8.31 +/- 0.77 and 5.05 +/- 0.45, respectively, compared to 13.18 +/- 1.69 (control). During the last period (40 to 60 min), the undisturbed animals recorded a 40 % decrease in ATP concentration, to reach 4.42 +/- 0.16 mu mol g(subscript)-1, while a 53% decline was recorded in the disturbed group, to 3.59 +/- 0.41 mu mol g(subscript)-1. During aerial exposure, the phosphagen reserve recorded a 55% decrease in the disturbed animals, at 4.82 +/- 1.37 mu mol g(subscript)-1, compared to 20% in the undisturbed group, at 8.64 +/- 0.87 mu mol g(subscript)-1. A 100% increase in lactate ion concentration was recorded in the muscle tissues of disturbed animals to reach 5.53 +/- 0.49 mu mol g(subscript)-1, compared to a 15% increase in the undisturbed group, at 2.83 +/- 0.29 mu mol g(subscript)-1.The monitoring of the physiological profiles of lobsters during extended periods (up to 72 hrs) of recovery in holding tanks revealed significant shifts in their physiological profiles and that a return to a steady state occurred only after 8 to 48 hours following re-immersion, according to the environmental conditions and the origin of the animals. Resting levels were identified after 24 hours for the haemolymph pH (close to 8.00), for the concentration in lactate, ions in the muscle tissues (2.00 to 3.00 mu mol g(subscript)-1 for the haemolymph glucose titre (0.30 to 0.60 mmol L(subscript)-1), for the haemolymph ammonia titre (close to 0.25 mmol L(subscript)-1), for ATP (6.50 to 7.70 mu mol g(subscript)-1), and for the phosphagen reserve (12.2 to 16.70 mu mol g(subscript)-1). Longer periods ([greater than]/= 48 hrs) were required for full recovery to occur when lobsters were stored at high stocking density and when lobsters were not isolated from their ++ / artificial environment. Extending the recovery period to 72 hrs resulted in slight changes in the physiological profiles of lobsters, with a 20 % decline in ATP/ADP, a 10% decrease in phosphagen concentration in the muscle tissues of the lobsters and subdued behavioural responses for those lobsters held at higher stocking densities.No attempt was made in the present study to establish the resting levels for the physiological variables surveyed. In a number of experiments, "control" groups have been studied and used as reference points to monitor changes accompanying exposure to a range of environmental conditions. The data pertaining to these different groups revealed a variability for most of the variables surveyed, suggesting that it would be hazardous to assume that these animals were undisturbed and to state with confidence that the values recorded for the variables surveyed would represent resting levels.The physiological responses of lobsters subjected to chilling procedures was investigated and significant physiological changes were identified. Short chilling procedures (3 and 6 min) were conducive of a dramatic reduction of the behavioural responses to handling, a reduction in pH values ranging between 0.13 and 0.24 of a unit, a rise in haemolymph lactate concentration by 1 to 2.6 mmol L(subscript)-1, a 20 to 100% increase in haemolymph glucose titre, a 35 to 75% decrease in phosphagen concentration. Extending the chilling period for up to 24 hrs resulted in a progressive return to control levels for most of the physiological variables surveyed. However, physiological signs of disturbance remained perceptible between 2 to 15 hours, as demonstrated by elevated lactate concentrations, lowered ATP and AP concentrations and lowered ATP/ADP values.Lobster body core temperature (CBT) reduction resulting from immersion in chilled water suggested that limited cooling ++ / effects were achieved by using the standard chilling procedures currently used by the WRL industry. Using "A" size lobsters (395 - 453 gr), a reduction of the CBT by 0.5 to 0.8 degrees celsius was recorded after 1 and 3 min immersion in 12 degrees celsius water, highlighting the limited low temperature effect exerted by these procedures in lowering the overall temperature of the mass of the product to be packed into export cartons.A study of the general physiological responses of lobsters to simulated live transport conditions in export cartons was conducted, investigating the effects of the period spent by the animals in export cartons, the effects of environmental temperature and the impact of chilling regimes. No attempt was made to duplicate exactly the conditions of cartons shipped overseas, that is carted by trucks to the airport and then transported by air to foreign markets. This study revealed that during the first 4 hours of transit, the animals exhibited physiological changes probably related to delayed responses to handling, disturbance and chilling procedures, as demonstrated by a decline in pH (0.1 to 0.3 of a unit), a rise in haemolymph ammonia (0.5 to 1 mmol L(subscript)-1) and glucose (0.5 mmol L(subscript)-1) titres, an increase in muscle lactate concentration (0.5 to 1 mu mol g(subscript)-1, a decrease in ATP concentration (1.5 mu mol g(subscript)-1, and a partial replenishment of the phosphagen reserve. These changes were less pronounced for those lobsters which underwent intermediate (30 min) chilling treatments.The subsequent periods (to 48 hours) were characterised, for all the treatments, by a rise in lactate concentration in the muscle tissues, this response being delayed for those lobsters which underwent a "6 min" or "30 min" chilling treatment. The data suggested that moderate changes in haemolymph lactate titre resulted from the increase ++ / in muscle lactate concentration, up to values ranging between 6 and 8 mu mol g(subscript)-1, beyond which levels, haemolymph lactate rose dramatically, to reach values up to 19.98 mmol L(subscript)-1. The ATP concentration remained relatively constant up to 18 - 26 hours, after which a steep decline was recorded to reach values below or close to 4 mu mol g(subscript)-1 after 42 hours, suggesting that the adenylate pool was maintained, probably through aerobic and anaerobic pathways of energy generation and by the "buffering" role played by the phosphagen reserve. After 24 to 36 hours, all the lobsters exhibited signs of energy depletion, as demonstrated by the changes in ATP/ADP ratio. A concomitant increase in lactate ion concentration and a decrease in haemolymph glucose titre was recorded, suggesting that anaerobic metabolism had become the major component of energy production. Simultaneously, a marked increase in the internal carton temperature was identified, which probably induced an increase in the metabolic rate of the lobsters. This "temperature effect" was delayed for up to 32-38 hrs transit, for those lobsters which underwent intermediate (30 min) and extended (24 hrs) chilling treatments. The changes in haemolymph pH and calcium titre suggest that the initial decline in pH identified at the completion of the first 4 hours of transit was, at least partially, compensated after 26 - 32 hours. A 30 to 40% increase in haemolymph calcium titre was recorded after 4 hours of transit, suggesting that bicarbonate ions were released in order to buffer the pH of the haemolymph. However, a decrease in pH (0.1 to 0.3 of a unit) was recorded during the subsequent periods suggesting that the bicarbonate buffering capacity did not suffice to match the recorded massive rise in lactate titre.By extending the duration of the chilling procedures and by using refrigerated ++ / material (wood-shaving fillers, ice-bottles), lower temperatures were achieved inside the packaging cartons and these were maintained for longer periods of transit (up to 20 hours), delaying the effect of the external environment on the temperature changes recorded inside the cartons and the concomitant metabolic responses of the animals. This effect was also achieved by maintaining the cartons in controlled temperature environments ([less than] 20 degrees celsius) and, to a lesser extent, by improving the insulation capacity of the polystyrene cartons.This study constitutes an overview of the physiological responses of Panulirus cygnus to post-harvest handling procedures currently used by the WRL industry. It revealed that an improved return for the Industry could be achieved by reducing the debilitating effects exerted on the lobsters by handling, exposure to air and elevated environmental temperature. It provides direction for future research, aimed at improving the quality and hence, the financial return in the live export of WRL.