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This investigation deals with the isolation and identification of bacteria found on selected samples of spoons in various eating establishments in the vicinity of Denton, Texas.

Poor sanitation infrastructure in rural areas can often lead to high levels of fecal contamination in local waterbodies and subsequent exposure to waterborne disease can occur. Although standard water quality measures such as quantification of E. coli can reveal relative concentrations of fecal contamination, they do not pinpoint the sources of such contamination. Source assessment in rural areas affected by untreated household waste might be improved with the human-specific, microbial source tracking marker HF183. This study attempted to quantify HF183 in two particular Appalachia streams with known discharges of untreated household waste. Water samples were taken above and at multiple points below these discharges on 29 occasions between August 2012 and April 2016, and tested for both HF183 and E. coli. HF183 was detected consistently in one of the study streams, though the concentrations were generally much lower than those previously reported in raw sewage; in the other watershed, HF183 was never detected. Further analysis via a multiple linear regression model showed a positive correlation between the level of E. coli and the proximity and number of known waste discharge points upstream from each sampling site. Primary conclusions of this study include: 1) HF183 is not always detected, even in watersheds with known sources of human fecal contamination, 2) it may be a useful water quality assessment tool where such contamination is suspected, particularly in cases where contaminant source allocation is necessary for setting mitigation priorities. / Master of Science

Yes / Since the building roof acts as hub for atmospheric sediment deposition, the attached microbes can enter rainwater storage tank with ease to cause health issue for rainwater users. This study aims to explore the trend of roof-top deposited microbes in the different areas of Ikorodu local Government Area at Lagos, Nigeria. This paper also tests the hypothesis that the roof sediment deposition being a significant source to home bacteria. The total deposition rate on the roof for 34 weeks, which include 17 rainy and 17 dry weeks, were investigated. The enumerated bacteria was obtained from the roof deposit samples, where four representative different sites have been analysed to study the solid depositions and microbes within the area thoroughly. The sites were selected judging by their levels of sanitation and vegetation rate. The experimental investigations showed that enumerated microbes and total suspended solid (TSS) depositions in the different areas were higher in the dry season compared to rainy season, and the highest deposition was experienced in the Harmattan period. In addition, the results showed that areas with poor drainage infrastructure and poor sanitation have the roof-top deposition with higher bacterial count than good sanitation areas; while the unpaved and unvegetated areas produced higher deposits than the paved and vegetated areas. In terms of contamination sources, this study has illustrated that the particulate deposition on the roof-top prior to collection has further contaminated the roof-harvested rainwater.

Yes / The coronavirus (COVID-19) pandemic coincides with the tenth anniversary of the recognition of the rights to water and sanitation within the United Nations system. Although water, sanitation and hygiene (WASH) remain critical for COVID-19 infection prevention and control, billions of people around the world lack access to basic WASH services in different spheres of life. Mostly affected are people living in vulnerable situations. While the pandemic has significantly impacted regulatory practices and access, key actors in the WASH sector continue to adopt diverse approaches to ensure safety, continuity, and reliability of supply. This chapter explores how COVID-19 influences WASH services and how the rights to water and sanitation can ultimately strengthen resilience to health pandemics? It makes recommendations from the perspective of inclusive development theory, for strengthening WASH sector governance towards ensuring the progressive realization of the rights to water and sanitation during and post the COVID-19 pandemic. Experiences with the coronavirus pandemic illustrate the crucial importance of access to water and sanitation as basic human rights and as necessities for the realization of health, education, food, gender equality, and other human rights (United Nations 2020). Emergent issues, particularly include the high public health risks associated with lack of water and sanitation and the disproportionate burden borne by women and girls, transgendered people, people living in informal settlements, people living with disabilities, the urban poor, migrant workers, workers in the informal sector, people who are sick or living with underlying health conditions, the elderly, school-aged children, and other groups living in vulnerable situations (Banerji 2020; Tan 2020; UNESCO n.d.). These highlight intersecting layers of inequalities in different situations of vulnerability and the interconnectedness of human rights. The pandemic has also demonstrated the imperative of leaving no one behind and ensuring universal access to water and sanitation to achieve sustainable development. From Africa to the Pan-European region, it is a similar picture: there are remarkable inequities in access to water and sanitation based on whether people live in urban or rural areas, whether people are rich or poor, and whether they have any special circumstances which render them vulnerable (Local Burden of Disease WaSH Collaborators 2020; Wang et al. 2019; World Health Organization & UN-Water 2019; United Nations 2020). Furthermore, because of the pandemic, several assumptions and modes of service delivery need to be reexamined to ensure continued suitability for promoting universal access to water and sanitation. It is in light of these realizations that this chapter examines the question: How has COVID-19 influenced water, sanitation and hygiene services and how can the rights to water and sanitation strengthen resilience in health pandemics? This question is addressed from the perspective of inclusive development theory which emphasizes the need to address the social, relational, and ecological aspects of human development (Gupta, Pouw, & Ros-Tonen 2015).

Yes / Global water security presents a complex problem for human societies and will become more acute as the impacts of climate change escalate. Water security connects the practical water and sanitation challenges of households to the dynamics of global hydroclimates and ecosystems in the Anthropocene. To ensure the successful deployment of attention and resources, it is necessary to identify the most pressing questions for water research. Here, we present the results of a scoping exercise conducted across the global water sector. More than 400 respondents submitted an excess of 4,000 potential questions. Drawing on expert analysis, we highlight 100 indicative research questions across six thematic domains: water and sanitation for human settlements; water and sanitation safety risk management; water security and scarcity; hydroclimate-ecosystem-Anthropocene dynamics; multi-level governance; and knowledge production. These questions offer an interdisciplinary and multi-scalar framework for guiding the nature and space of water research for the coming decades.

No / The current Intergovernmental Panel on Climate Change (IPCC) report reemphasises the fact that the African continent is particularly vulnerable to the disastrous impact of climate change. The Report highlights the urgent need for countries to adopt critical strategies towards ensuring the actualisation of 1.5 C temperature. Based on low adaptive strategies and geographical location, the impacts of climate change further exacerbate the inherent environmental challenges of the African continent, more so, the struggle to attain poverty-eradication. One of the adverse impacts of climate change is reducing access to water, thus increasing the burden on already water scarce African countries such as South Africa and Nigeria. In cognizance of the fact that access to water and sanitation is critical towards poverty eradication and realising the kind of continent that Africans want by 2063, African countries are increasingly adopting several measures to ensure citizens’ access to water. Taking a regional approach, this paper examines the current institutions and legal framework adopted by regional bodies in Africa, namely the South African Development Community (SADC) and the Economic Community of West African States (ECOWAS), proffering a practical template that States in sub-Saharan Africa can adopt to resolve this challenge of lack of access to clean, safe drinking water and basic sanitation. The chapter also discusses the existence of an adequate enforcement mechanism that ensures and provides the platform for citizens to assert and protect their right to access clean water and sanitation. Furthermore, the chapter’s focus on South Africa and Nigeria, analyses the extent to which regional frameworks are being adopted, implemented, and sustained at the national level, proffering recommendations which can be adopted by other countries within the sub-Saharan African region.

Providing clean water remains a challenge in many African countries. Ethiopia, with the second largest population in Africa is also faced with this predicament. Efforts to improve supply have focused on abstracting groundwater. Although relatively cheaper to utilize, groundwater is prone to contamination, from improperly disposed of waste, particularly urban areas with no appropriate sanitation services. The city of Addis Ababa is faced with this difficult situation. Currently about 75 % of the population of Addis Ababa has access to sanitation in the form of pit latrines, while 0.6 % has access to sewerage services. The rest of the population is considered to have no access. This proliferation of pit latrines in the city has enhanced the risk of groundwater contamination. This research was initiated with the objective of assessing the temporal and spatial extent of contamination of groundwater due to human waste. It attempted to review the quality of water in deep wells and springs. The data analyzed indicates that the temporal and spatial extent of contamination has increased over the past few decades. Over abstraction of groundwater has also been observed. Whilst contaminant levels such as nitrates and chlorides in many wells are below maximum permissible values, few wells in the centre of the city have exhibited higher values. This steady temporal increase may soon make some wells unsuitable for human consumption. Efforts to reduce this risk will need to focus on sewerage services provision, review of existing environmental policy, public awareness drive and sustainable groundwater management. / D.Phil. (Environmental Science)