Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-10-13T17:45:33Z No. of bitstreams: 2 Dissertação - Luciano Ferreira da Silva - 2016.pdf: 1661946 bytes, checksum: 28fb8508d545a9e09562fc653abfc880 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-10-13T17:45:33Z (GMT). No. of bitstreams: 2 Dissertação - Luciano Ferreira da Silva - 2016.pdf: 1661946 bytes, checksum: 28fb8508d545a9e09562fc653abfc880 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-09-05 / Although the search for strenghtening regulation and control had only been explicited in the 1995 with the Managerial Reform of Ministry of Government Administration and Reform (MARE), through State's managerial reform master plan, the basic sanitation sector only achieved federal regulatory landmark with the 11.445/2007 law. This law has brought universal access to services as one of the fundamental principles and objectives pursued by organizations related to this sector, which, after analyzing financial resources needed, estimated by the Ministry of Cities in 2013, would be inconceivable without the participation of private capital. On the other hand, introducing private capital necessarily improves regulatory action, once the more transparent and stable are the rules and mechanisms behind regulatory agencies, the greater are the chances of return to investors and the impacts on cost of companies’ capital are smaller. Thus, assessment of risk impacts derived from the action of regulatory agencies is of fundamental importance to the awareness of the need for greater transparency predictability and stability of the rules. Thus, whereas the Basic Sanitation Company of the State of São Paulo (Sabesp), is commonly used as a benchmark for the sector, among other reasons because it is the largest segment, this study examined the effects of Resolutions of the Regulatory Agency of sanitation and Energy of the State of São Paulo (Asesp), the regulatory events, of economic and financial nature, from 2007 to 2015, on the clusters of volatility of return of sanitation Company shares Basico do Estado de Sao Paulo (Sabesp ) by the method TGARCH (Threshold Generalized Autoregressive Conditional Heteroscedastic). The results constitute evidence that the regulatory agency decisions can influence the volatility when it generates change in expectations and may cause changes in the risk and cost of capital. At the end has crafted a diagnosis that considered correct use of the CAPM and WACC to define the cost of capital, but pointed out that the decision of Arsesp in not recognizing a specific component for regulatory risk was inadequate. Recommends to the next tariff review cycle of Sabesp, the assessment by the Arsesp of possibility of including a specific component to reward investors due to regulatory risk. / Embora a busca pelo fortalecimento das funções de regulação e controle tenha sido explicitada em 1995, com a reforma gerencial, pelo Ministério da Administração Federal e Reforma do Estado (MARE), por meio do Plano Diretor da Reforma do Aparelho do Estado, o setor de saneamento básico só veio a contar com um marco regulatório federal, a Lei nº 11.445, em 2007. Essa lei trouxe como um dos princípios fundamentais e objetivo perseguido pelas organizações do setor a universalização do acesso aos serviços, algo que, pelo montante de recursos financeiros necessários, estimado pelo Ministério das Cidades em 2013, seria inconcebível sem a participação do capital privado. Por outro lado, a atração do capital passa necessariamente pela melhoria da ação regulatória, pois quanto mais transparente e estável forem as regras e os mecanismos de atuação das agências reguladoras, maiores serão as chances de retorno aos investidores e menores serão os impactos causados no custo de capital. Assim, a avaliação dos impactos decorrentes do risco derivado da atuação das agências reguladoras é de fundamental importância para a sensibilização quanto a necessidade de maior transparência previsibilidade e estabilidade das regras. Desse modo, considerando que a Companhia de Saneamento Básico do Estado de São Paulo (Sabesp), é comumente utilizada como um benchmark para o setor, dentre outras razões porque é a maior do segmento, este trabalho analisou os efeitos das Deliberações da Agência Reguladora de Saneamento e Energia do Estado de São Paulo (Asesp), os eventos regulatórios, de natureza econômico-financeira, no período de 2007 a 2015, sobre os clusters de volatilidade do retorno das ações da Companhia de Saneamento Básico do Estado de São Paulo (Sabesp), por meio da metodologia TGARCH (Threshold Generalized Autoregressive Conditional Heteroscedastic). Os resultados constituem-se em evidências de que as decisões da agência reguladora podem influenciar a volatilidade quando gera modificação nas expectativas, podendo provocar alterações no risco e no custo de capital. Ao final foi elaborado um diagnóstico que considerou correta a utilização do CAPM e da metodologia WACC para a definição do custo de capital, mas apontou que a decisão da Arsesp em não reconhecer um componente específico para o risco regulatório foi inadequada. Recomenda para o próximo ciclo de revisão tarifária da Sabesp, a avaliação por parte da Arsesp da possibilidade de inclusão de um componente específico para premiar o investidor em razão do risco regulatório.

Ao longo dos anos a implementação das políticas de saneamento básico no Brasil foram evoluindo gradativamente, porém as metas de universalização não serão alcançadas até 2030. Os piores índices historicamente se concentram nas regiões norte e nordeste, sendo mais acentuados nas áreas rurais. O Amapá é o estado mais preservado do país, abrange em seu território diversas modalidades de áreas protegidas e comunidades tradicionais, dentre elas a comunidade Água Branca do Cajari, localizada na Reserva Extrativista do Rio Cajari, objeto de estudo desta Dissertação, que consistiu na formulação e avaliação de alternativas para suprir a demanda de água potável e ao encaminhamento seguro dos esgotos sanitários. Para o alcance dos objetivos, foram realizadas duas campanhas de campo, com o intuito de conhecer o perfil social, econômico e ambiental da comunidade, através da aplicação de questionários e observação in situ, avaliação da qualidade das águas destinadas ao consumo humano, através de análises físico-químicas e microbiológicas e estimativas da geração de esgoto. A proposição das alternativas foi feita a partir do dimensionamento e avaliação dos custos de instalação, operação e manutenção ao longo do horizonte de planejamento de 20 anos. Os orçamentos foram elaborados a partir de dados disponíveis na literatura técnica e a composição dos custos por pesquisas de preço e fontes oficiais como o SINAPI. Treze poços freáticos são utilizados pela população que reside na parte alta da comunidade, enquanto os que moram na parte baixa, retiram diretamente do rio, adicionam polímero que adquirem informalmente para a clarificação das águas, em média 95% da população afirma realizar a desinfecção com hipoclorito de sódio, sendo que 41,1% da população tem acesso ao mínimo estabelecido pela ONU e 7,6% abaixo do recomendado. Estavam em não conformidade, de acordo como o que estabelece a Portaria n° 2.914/2011 do Ministério da Saúde, 75% das amostras analisadas para o parâmetro pH, 62,5% para turbidez, 90% para coliformes totais/Escherichia coli. Cerca de 82,3% do esgoto séptico gerado é encaminhado a fossas negras, e 13% das unidades domiciliares não dispõe de banheiro. O custo total dos sistemas foi de: sistema de bombeamento fotovoltaico 430.644,97 R$ (0,18 R$/hab.dia); aproveitamento de água de chuva de 432.236,72 R$ (0,19 R$/hab.dia); filtro lento domiciliar 7.326.27 R$; sistema de desinfecção solar (SODIS) 9.768,54 R$ e para o tratamento de esgoto sanitário unifamiliar, fossas sépticas \"Imhoff\" e sumidouro 434.371,32 R$ ou 0,19 R$/hab.dia. Portanto, verificou-se que as alternativas avaliadas são viáveis economicamente e operacionalmente. A efetividade da implantação das tecnologias de tratamento de água e esgoto em comunidades isoladas ou de difícil acesso na Amazônia devem ser precedidas de estudos socioeconômicos, ambientais e culturais. / In the world, about 748 million of people do not have access to a potable water, more than 30% of the world population do not have appropriate installations and 1 million of people do their physical necessities in open areas, of theses, nine out ten are in rural areas. The policies about basic sanitation in Brazil gets better long of the years, but the marks for universalization cannot be touched until 2030. The indications get worst in north and northeast, especially in the countryside. Amapá is the most preserved state of Brazil, that has in its territory many spaces of protected areas and traditional communities, one of them is Àgua Branca do Cajarí, located in an extractive reserve, and is the focus of this issue, whose objective is propose and estimate better ways to get a high quality of water and sanitary wastewaters. To get better results, it was necessary to visit at these areas to know the community social, economic and environmental profile through questionnaires and in situ observation, beside doing physic-chemical and microbiological analyzes. The proposition of the best alternatives was made by starting from the estimative of installation and conservation along of 20 years, getting the present prizes. Those estimative were based on technical literature, prizes researched and official sources as SINAPI. Thirteen phreatic wells are used by people who live on the higher part of the community while people that live on the lowest part need to take water right from the river, put some polymer that is taken informally to clarify the water. About 95% of people say that use sodium hypochlorite in water, but just 41% has the least required by UNO and 7.6% is low from what is demanded. According with the governmental decree N. 2914/2011, 75% of the sample that were analyzed showed to the pH parameter; 62.5% for turbidity; 90% for total coliforms/ Escherichia coli. About 82.3% of the septic sewer created is sent to black cesspool and 13% of the houses do not have a bathroom. The total cost of the systems was about R$ 430,644.97 for a photoelectric system (0.18 R$/resident.day). The perforation and preservation of the wells were not considered; R$ 432,236.72 if use rain water (0.19 R$/resident.day). R$ 7,326.27 for slow domiciliary filter. R$ 9,768.54 for solar disinfection system and R$ 434,371.32 for unifamilial sanitary sewer treatment, septic tank \"imhoff\" and escape hole (0.19 R$/resident.day). Therefore, it was possible to see that the alternatives analyzed were economically and operationally possible. The implementation of water and sewer treatment technologies in isolated communities of by difficult access in Amazonia must be followed by socioeconomic, environmental and cultural studies.

Numerous informal houses have been built on and adjacent to a landfill in iBayi, Port Elizabeth, South Africa, which accepted domestic and industrial waste. Formal housing surrounds most of the site at a greater distance - some 60 m, or further, from the landfill. Both formal and informally housed residents complain of odours, burning eyes, sore throats and headaches - symptoms which they believed were caused by the landfill. The landfill gas and ambient air were analyzed to classify and quantify the VOCs (volatile organic compounds) emitted and then to compare the quantitative data with recognised standards to establish if the residents are at risk. Eighteen target (potentially hazardous) VOCs were quantified. A wide variety of compounds were detected in both the ambient air and landfill gas. The results of the VOC analyses were similar to those of other workers in both the qualitative and quantitative studies. The concentrations of the VOCs were mostly lower than the TLV (threshold limit values) values, but exceeded the MRLs (minimum recommended levels). The combined concentrations of the VOC’s in the ambient air either approached or exceeded the limit values for combined exposure thus indicating that a potential health hazard exists. One third of the VOCs were detected in both the ambient air and the subsurface gas, however, external pollution sources also appear to contribute to the VOC concentrations ambient air. Dangerously high methane concentrations were repeatedly detected in the landfill gas amongst the informal houses. There was a vast improvement in the aesthetic qualities of the landfill since the disposal restriction to accept only domestic refuse and building rubble in July 1997. The ambient air was less odorous and landfill site littered. Fewer informal recyclers were present and their concomitant squabbling over valuables had almost vanished. The management of the iBayi landfill holds much room for improvement. There is potential for serious injury or even death if no action is taken to remedy the problems at the iBayi landfill. A holistic solution will have to be found to make the landfill a safe neighbour. Some complementary analyses (such as pH, heavy metal concentrations in the water and sediments etc.) were performed on the leachate and water surrounding the landfill.

The Bangladeshi capital, Dhaka, is the world’s fastest growing primate city, having nearly 15 million people and approximately 6 million living in slum areas. Their high population density and growth rates, coupled with inadequate and inappropriate water and sanitation (WatSan) facilities, are creating social, economic and environmental effects. Until recently, several attempts have been made to provide infrastructure services to those slums. But the extent of the services is unsatisfactory due to resource constraints and a burdensome concentration of slums that contaminates the city ecology on a broad-spectrum. In consequence, the trend of development ventures through government (GO) and non-government organizations (NGO) is not only disappointing but also questionable due to disastrous project histories. The complex social dynamics of these informal settlements, together with inappropriate or inadequate WatSan facilities and incompetent governance systems obstruct the pace of sanitation interventions. Apart from this, Bangladesh has succumbed to political indiscretion and bureaucratic intemperance which have severely diminished the capacity of the GOs and NGOs to perform at a reasonable level. The result is all round deterioration in the quality and adequacy of the urban basic services and people of the informal settlements are the worst sufferers. It is widely recognized that the poor communities mostly have no proven demand for improved sanitation facilities, as their basic priority, rather, is managing their next meal. In this situation, some NGOs have come forward with their ‘flexible’ and ‘tailor-made’ working strategies developed from previous project experiences whereas government agencies are more geared to ‘facilitation’ and continue with their ‘supply-driven' strategy, ignoring criticisms and pitfalls. As one of the most dysfunctional sectors in Bangladesh, urban sanitation is traumatized and its coverage is affected by several interconnecting factors while the government continues to bypass questions related to slum improvement arguing that the slums are illegal settlements and do not qualify for government services. Several NGOs have come forward to work in the urban sanitation sector and in most instances, the poor slum-dwellers have appreciated the NGOs’ participatory working strategies. In fact the dynamics of the ‘social-technological-governance’ system of the slum areas often determines the success of sanitation interventions. In this research, the vibrant dynamics of ‘social-technological-governance’ systems and the roles of GO-NGO service providers and beneficiaries in the selected slums are critically analysed through a qualitative methodology and a bottom-up approach that has the potential to identify inherent policy weaknesses and factors that facilitate or hinder the successful implementation of sanitation programmes. This research is entirely based on empirical evidences and the qualitative assessment of field data that were collected from five informal settlements of Dhaka city and associated GO-NGO sources. The outcome of this research suggests that the impacting factors are not equally weighted in WatSan projects as some could be defined as crucial and influencing factors that shape other interrelated factors. In order to smoothen the pathways of different WatSan projects it is necessary to carefully identify and restrict those problem-breeding factors on a priority basis. This research also describes different stakeholders’ practices and links with existing policies to identify the gaps between them. Here, the proposals are made for reality-based, short-term and long-term solutions and policy recommendations that might offer guidelines for addressing the overwhelming slum sanitation agenda in urban Bangladesh.

No abstract available. / Dissertation (LLM)--University of Pretoria, 2013. / gm2014 / Centre for Human Rights / unrestricted

Magister Artium (Development Studies) - MA(DVS) / The purpose of this study was to examine the level and extent of community participation in the Danga Ecological Sanitation Project carried out in the Zvishavane district of Zimbabwe. The people-centered approach was chosen as a theoretical background. Both quantitative and qualitative methods were used to gather relevant information. The results indicated that the community was not fully involved in the ecological sanitation project. As a result, the project had a poor performance record. In the course of this study, political interference in community projects carried out in Zimbabwean rural communities, resulting in the failure to reach the intended beneficiaries, was also noted. Full community participation in community projects may ensure that empowerment and ownership take place. Institutional arrangements, which in most cases impede development, need to be readdressed with clear demarcation of decision-making processes. / South Africa

Background: The cholera epidemic experienced in the province of KwaZulu-¬Natal between August 2000 and July 2001 resulted in 105, 000 reported cases of cholera and 220 human deaths. Objectives: The primary objective of this study was to make comparison between districts with cholera and districts with no cholera in rural KwaZulu-Natal on the basis of known risk factors for cholera and diarrhoeal diseases. Comparison was made with regards to key factors such as the prevalence of diarrhoeal diseases, the provision of safe water supply, ownership and effective utilization of sanitary facilities, knowledge and practice on water purification and prevention of cholera. Methods and materials: This was a descriptive, cross- sectional, ecological and comparative study among households of KwaZulu-Natal. The communities were stratified into two groups. One group had cholera (Group 1) and the other group (Group 2) had no cholera. Thirty communities were selected by systematic random selection from each of the two groups. From each community, eligible households were selected using simple random sampling technique. Trained field workers used a pre-tested questionnaire to collect data during the months of November and December 2001. Statistical procedures such as two-sample tests on means and proportions, Pearson's chi-square tests of association, odds ratios, binary logistic regression analysis, sensitivity tests, specificity tests and ROC (receiver of characteristics) analysis were used for data analysis. Results: A total of 1420 households from both groups were included in the study. The response rate for Group 1 and Group 2 was 84%,92%. Female respondents (70%) predominated male respondents (30%). Tap water supply was less common in Group 1 (54%) than in Group 2 (72%), (p< 0.05). A higher percentage of households in Group 1 (27%) used dam or river water compared to Group 2 (20%) (p<0.05). Household knowledge on water purification by boiling was (71%) in Group 1 and (87%) (p<0.05) in Group 2.38% of households in Group 1 practiced water purification by using disinfectant JIK. The corresponding figure for Group 2 was 50% (p<0.05). Groups 1 and 2 were similar with respect to ownership of toilets (84% and 85% respectively). Groups 1 and 2 showed a marked difference with respect to utilization of toilet facilities by all family members (70% and 89% respectively). The prevalence of diarrhoeal diseases for Group 1 was higher (14.3%) (p<0.05) than the Group 2 was (11.1%). Factors found to be helpful for protection against diarrhoeal diseases were the boiling of water (OR=0.41, 95% CI, 0.19-0.90) and the use of disinfectant JIK (OR=0.45, 95% CI, 0.19-0.94). The study also showed that the use of dam or river water was significantly associated with diarrhoeal diseases (OR=2.92, 95% CI, 1.06-7.80). Conclusion: The results showed that there was significant difference between the two groups of households in regards to basic provision of safe water, knowledge and practice of good hygiene, ownership and effective utilization of sanitary facilities. Findings from this study could be useful as baseline information for future planning, monitoring and evaluation of ongoing programmes. / Dissertation (MSc(Epidemiology))--University of Pretoria, 2005. / School of Health Systems and Public Health (SHSPH) / unrestricted

The issues concerning women and their participation (or rather lack thereof) in the development process have been increasingly examined over the last few decades. The interpretation of women's roles and gender relations, especially in water supply and sanitation projects, have been marked by shifting positions and changing political priorities over the last few decades. This study contributes to the knowledge regarding issues surrounding the roles and equality of women and men in water supply and sanitation projects in the Eastern Cape Province. It provides a background to the origins and development of gender and gender mainstreaming in the developing world in relation to the changing roles and responsibilities of women in water supply and sanitation projects. The roles and responsibilities of women, men and children are closely interlinked with their cultural perceptions, the way they grow up and the way they are brought up within their cultural environment and relationships with people close to them. The key to understanding how development work affects women, men, girls and boys, is in grasping the concept of gender. The term "gender" refers to those characteristics of women and men that are socially determined. This dissertation discusses gender-awareness approaches in development projects such as water supply and sanitation and the effect these projects have had to date on the empowerment, position and roles of women. The research in the Eastern Cape Province for this study is one of only a few case studies which could be identified in South Africa. This research and the case studies illustrate that development in South Africa needs to be made gender aware and gender sensitive, and that the mainstreaming of gender in South Africa is a long way behind the rest of the developing world. / Dissertation (MA (Antropology))--University of Pretoria, 2006. / Anthropology and Archaeology / unrestricted

The objectives of this study were to: assess the sanitation conditions and sustainability of sanitation technologies, using a comprehensive framework of sustainability indicators. A survey of 500 households and focus group discussions of 40 key informants were conducted in five villages. Ninety-six percent of the households had toilets, with only 9.4% having improved toilets. A strong relationship existed between the percent of sanitation coverage and the diarrhea incidence rates. Education and family wealth were the two significant determinants of sanitation coverage. On the basis of the sustainable development index, SanPlat and VIP latrine were the top two probable sustainable technologies. Variation did exist in the rankings of sanitation technologies across the study villages. Improvement in sanitation in rural Tanzania requires education of people about its health benefits, proper training, and extension of rural health workers

Orientador: Mirna Lucia Gigante / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-30T23:01:21Z (GMT). No. of bitstreams: 1 Ribeiro_MariaCeciliaEnes_D.pdf: 17038739 bytes, checksum: 54c8350faac4b14e773283e6e871aa78 (MD5) Previous issue date: 2015 / Resumo: O objetivo geral deste trabalho foi avaliar o efeito de diferentes matrizes na adesão e formação de biofilme em aço inoxidável por Bacillus cereus, bem como avaliar a eficiência dos procedimentos de higienização no controle de biofilmes de esporos desse micro-organismo. Nas duas primeiras etapas, avaliou-se a capacidade de adesão e formação de biofilme por B. cereus em aço inoxidável, com e sem prévio condicionamento da superfície, utilizando-se água, leite UHT desnatado e integral como matrizes e quatro diferentes tipos de inóculos, pool de células vegetativas de B. cereus isolados da indústria láctea, pool de esporos de B. cereus isolados da indústria láctea, células vegetativas da cepa de B. cereus ATCC 14579 e esporos da cepa de B. cereus ATCC 14579. Na terceira etapa do trabalho avaliou-se a influência da matriz condicionante (água e leite UHT integral), do meio de inoculação do pool de esporos de B. cereus (água e leite UHT integral) e do tempo de exposição (5 min (0,08h), 10, 24, 48 e 72 horas) sobre a adesão e formação de biofilme por B. cereus em aço inoxidável. Na quarta etapa, avaliou-se a eficiência de nove procedimentos de higienização na remoção dos biofilmes formados pelo pool de esporos de B. cereus em aço inoxidável. Todos os experimentos foram repetidos três vezes e os dados estatisticamente avaliados. A hidrofobicidade e o potencial zeta das superfícies dos esporos também foram avaliados. Os resultados das duas primeiras etapas indicaram que o pool de esporos de B. cereus isolados de indústria láctea apresentou a maior capacidade de adesão e formação de biofilme em aço inoxidável quando comparado aos outros tipos de inóculos, em todas as condições avaliadas. O maior grau de adesão de esporos de B. cereus (4,93 log UFC/cm2) foi observado ao se utilizar leite integral como matriz condicionante do aço inoxidável. Entretanto, comparando-se todas as matrizes, a menor adesão (3,01 log UFC/cm2) foi observada quando o pool de esporos de B cereus foi veiculado no leite integral sem prévio condicionamento da superfície. Na terceira etapa do trabalho observou-se que a adesão e formação de biofilme pelo pool de esporos de B. cereus foi maior quando inoculados em água, independente das matrizes de condicionamento. A adesão de B. cereus aumentou 1,02 e 0,3 log UFC/cm2 ao longo do tempo de exposição, quando o pool de esporos de B. cereus foi inoculado em água e leite integral, respectivamente. O biofilme de esporos veiculados na água apresentou maior resistência aos procedimentos de higienização. A sanitização com hipoclorito de sódio foi mais eficiente na remoção dos biofilmes quando comparada ao ácido peracético. O pool de esporos de B. cereus isolados da indústria láctea foi altamente hidrofóbico e apresentou carga negativa em uma ampla faixa de pH, com ponto isoelétrico de aproximadamente 3,0. Os esporos de B. cereus isolados da indústria láctea apresentaram maior capacidade de adesão ao aço inoxidável quando comparados aos outros inóculos avaliados, o que pode estar relacionado à alta hidrofobicidade e a baixa carga de superfície dos esporos / Abstract: The aim of this study was to evaluate the effect of different matrices on the adhesion and biofilm formation by Bacillus cereus on stainless steel, and to evaluate the effectiveness of sanitation procedures for controlling biofilm from spores of this microorganism. The first two parts were carried out in order to evaluate the adhesion and biofilm formation by B. cereus on stainless steel, with and without previous conditioning of the surface, using water, skim and whole UHT milk as matrices and four different types of inocula: a pool of B. cereus vegetative cells isolated from dairy industry, a pool of B. cereus spores isolated from dairy industry, vegetative cells of B. cereus ATCC 14579, and spores of B. cereus ATCC 14579. The third part of the study evaluated the effect of the conditioning matrix (water and whole UHT milk), the inoculation medium of pool of B. cereus spores (water and whole UHT milk) and exposure time (5 min (0.08h), 10, 24, 48 and 72 hours) on the adhesion and biofilm formation by B. cereus on stainless steel. In the fourth part, the effect of nine sanitation procedures on the removal of B. cereus spores biofilm was evaluated. All experiments were repeated three times and data were statistically evaluated. Hydrophobicity and zeta potential from spore¿s surface were also evaluated. Regarding the results to the first and second parts, the pool of B. cereus spores isolated from dairy industry had the highest ability of adhesion on stainless steel when compared to the other inocula, for all tested conditions. After stainless steel surface conditioning with whole milk, B. cereus spores showed the highest adhesion (4.93 log CFU/cm2). However, lower adhesion (3.01 log CFU/cm2) was observed when B. cereus spores were delivered in whole milk as compared to the other matrices, without previous conditioning of the surface. The results of the third part indicated that the adhesion and biofilm formation by the pool of B. cereus spores was higher when they were inoculated in water, regardless of the conditioning matrix. B. cereus spores adhesion increased by 1.02 and 0.3 log CFU/cm2 over exposure time, when the pool of B. cereus spores was inoculated into water and whole milk, respectively. Biofilm of B. cereus spores inoculated in water showed the highest resistance against all tested sanitation procedures. Sodium hypochlorite was the most effective sanitizer for removing all biofilms when compared to the peracetic acid. The pool of B. cereus spores isolated from dairy industry was highly hydrophobic and showed a negative charge at a wide pH range, with an isoelectric point of about 3.0. B. cereus spores isolated from dairy industry showed the highest ability to adhere on stainless steel when compared to the other inocula, which is possibly related to its higher hydrophobicity and lower spore surface charge / Doutorado / Tecnologia de Alimentos / Doutora em Tecnologia de Alimentos