Three species of woody plants, Ilex crenata 'Helleri', Juniperus chinensis procumbens 'Nana', and Ligustrum lucidum, were grown in one-liter containers filled with pine bark to determine Fe and Mn requirements with regard to rate and ratio. FeEDTA and MnEDTA were applied at either a 2:1 or 1:2 ratio of Fe:Mn at 5 concentrations each, 3 times per week with each irrigation. Medium solutions were collected every 21 days on one species and analyzed for Fe and Mn levels. Dry weight and tissue Fe and Mn levels were determined for all three species. Neither rate nor ratio of applied Fe and Mn had an effect on shoot dry weights. Control treatments, in general, had the lowest medium solution and tissue levels of Fe and Mn, however, there was no difference in dry weights between control and treatment plants. These results suggest that pine bark supplies adequate levels of Fe and Mn for growth under the conditions of this study. In a second study, three sources of Fe and Mn were applied to Tagetes erecta 'Inca' growing in 500 cc plastic pots containing sieved pine bark at 3 lime rates: 0, 3, and 6 kg m⁻³. Sources of Fe and Mn were pre-plant Micromax, liquid sulfate salts, and liquid chelates applied in the irrigation water. No difference in growth between micronutrient sources was detected, however, growth was greater at the 3 and 6 kg m⁻³ lime rates. Levels of Fe and Mn in medium solution and tissue decreased with increasing lime rate, with availability of Fe and Mn greatest with chelate as the source, regardless of lime rate. A similar study was conducted with a control and liquid sulfate treatment. There was no difference in dry weight between the sulfate treatment and the control, except at 0 kg m⁻³ lime where the control plants were larger. Again, lime additions increased growth, and Fe and Mn availability in medium solution and tissue levels decreased. These results suggest that if Fe and Mn additions are needed, all sources provide adequate Fe and Mn for growth. / M.S.

The effect of date and method of lifting on ethylene production by 1-0 loblolly pine (Pinus taeda L.) seedlings during cold storage, the dose-response relationship between ethylene and loblolly pine seedlings during cold storage, and the effect of water stress on ethylene and aminocyclopropane-1-carboxylic acid (ACC) production in two half-sib loblolly families were investigated. Seedlings stored in Kraft-Polvethvlene (K-P) bags showed a general trend of increasing ethylene concentrations from November through February, with an abrupt drop in March. Production rates may be related to the level of dormancy of the seedlings, with the peak in production corresponding to fulfillment of the chilling requirement. Ethylene concentrations within the K-P bags generally declined over the twelve weeks in cold storage. Roots produced significantly higher levels of ethylene while stored in the K-P bags; however, when incubated under light, the needles produced higher concentrations. Roots of machine-lifted seedlings produced significantly higher levels of ethylene than roots of hand-lifted seedlings. However, rates tended to moderate during storage and differences in production between HL and ML whole seedlings were not significant, which suggests that mechanical lifting is not a source of increased ethylene production. A dose-response study indicated that ethylene fumigation during cold storage tended to slightly enhance growth of outplanted seedlings. The effects of water stress on a Virginia Coastal Plain (CP) and an East Texas Drought Hardy (DH) loblolly family were also investigated. Ethylene production during severe stress (-2.8 MPa) appeared to be related to drought hardiness, with the CP seedlings producing much higher levels. Roots of both families produced greater levels of ethylene than the needles and may be due to an enhanced ability to convert 1-Aminocyclopropane-1-carboxylic acid (ACC) to ethylene. / Master of Science

A two year study examined Root Growth Potential (RGP) in a hydroponic system and Dormancy Release Index (DRI) in one-year-old (1-0) and two-year-old (2-0) eastern white pine (Pinus strobus L.), red pine (Pinus resinosa Ait.), and Scotch pine (Pinus sylvestris L.) seedlings with emphasis on eastern white pine. During 1985-86 all three species of 2-0 stock displayed a similar pattern of RGP with high levels of RGP limited to mid-March. Only 2-0 eastern white pine had a statistically significant relationship between RGP and DRI; however, this relationship was not consistent in 1986-87. The RGP/DRI relationship in 2-0 eastern white pine was strong over both years within a DRI range of 0.22 to 1.00. One-year-old and 2-0 eastern white pine stock had very different patterns of RGP over both years with 1-0 stock maintaining much wider "lifting windows" for high RGP. Cold storage (2C) of 2-0 eastern white pine resulted in variable effects on RGP although it typically progressed dormancy release. There were no strong RGP trends between northern and southern provenances of 1-0 eastern white pine and heritability values indicate that RGP is under minimal genetic control in 1-0 eastern white pine seedlings. Northern and southern provenances did display clear differences in seedling morphology and seasonal patterns of shoot activity. Results of a field outplanting study show that RGP does show promise as a measure of 2-0 eastern white pine seedling quality. The predictive ability of RGP was increased on non-irrigated versus irrigated seedlings. A comparison of greenhouse versus growthroorn RGP testing demonstrated the validity of using greenhouse RGP testing of 2-0 eastern white pine. / M.S.

Root growth potential (RGP), the ability of a transplanted seedling to rapidly elongate existing roots and initiate new roots, is used as an indicator of seedling physiological quality. Three separate experiments were conducted on loblolly pine RGP during 1983-84 and 1984-85 using 1-0 nursery-grown seedlings. In the first study, seedlings were hand-lifted from September to April at 17-day intervals in 1983-84 and 7-day intervals in 1984-85. The RGP variation over time was related to environmental and seedling phenological changes. In addition, the new root systems were morphologically described. In the second study, changes in loblolly pine seedlings following different lift-date X cold storage duration treatments were described. These changes were compared to the RGP, shoot activity, and new root system morphology of fresh-lifted seedlings. Genetic variation in RGP on different lift-dates was quantified in the third study. Variation patterns over time among 14 half-sib loblolly pine families hand-lifted at one-month intervals were examined. Root growth potential was low in the autumn until the cessation of shoot activity. Seedlings stored poorly if put in cold storage during this time, or prior to the accumulation of at least 400 chilling hours. Seedling RGP increased in the early-winter to a late-February peak, and then decreased when spring shoot elongation occurred during the RGP tests. Similar trends over time were noted with seedlings removed from cold storage during these times. The RGP pattern over time was consistent among years and among the half-sib families, although there were some minor differences among half-sib families in the rate of increase to, and decrease from, the late-winter peak. Most new roots resulted from elongation of existing roots rather than initiation of new roots regardless of lift-date; however, when seedling metabolism was high (early autumn and late-winter) both root initiation and elongation of existing roots contributed to the new root system. In addition, the morphology of root systems of cold-stored seedlings was similar to fresh-lifted seedlings. Differences in numbers and lengths of new roots among half-sib families were due to the differential ability to produce new roots through both root initiation and elongation. / Ph. D. / incomplete_metadata

The effect of moisture stress conditioning on the drought tolerance and performance of three open-pollinated families of loblolly pine (Pinus taeda I.) during water stress were investigated. Seedlings were subjected to prolonged sublethal drought treatments which included a watered control, a moderate stress treatment (MWS, seedlings watered only when needle water potential reached -0.8 MPa) and a severe stress treatment (SWS, seedlings watered only when water potential reached -1.4 MPa). After the conditioning period, numerous physiological and morphological parameters were measured, and performance of seedlings during water stress evaluated. Significant decreases in needle osmotic potential occurred in moisture stress conditioned seedlings. As a result, turgor in conditioned seedlings was equal to or greater than control seedlings even at lower needle water potentials. Photosynthesis vas decreased greatly with reduced needle water potential. However, the MWS and SWS seedlings maintained photosynthesis to water potentials 0.15 and 0.45 MPa lower than control seedlings, respectively. This response is likely the result of both osmotic adjustment, and an acclimation of the photosynthetic process resulting in less non-stomatal inhibition of photosynthesis at low needle water potentials. Initial needle conductance and transpiration, but not photosynthesis, were reduced greatly by the conditioning treatments, and resulted in improved water-use efficiency in conditioned seedlings. The response of stomata to changing vapor pressure deficit was increased through moisture stress conditioning. Boot growth was affected more by moisture stress than shoot growth, causing a decrease in root/shoot ratio. Changes in root morphology as a result of conditioning are not likely to improve the drought tolerance of loblolly pine seedlings. SWS conditioning significantly improved the height growth increment and resulted in slightly greater shoot and root biomass of outplanted, containerized seedlings after the first growing season, despite these seedlings being much smaller at the time of planting. Differences between seed sources did occur in the experiments, with a source from Texas generally showing the least response to moisture stress conditioning. / Ph. D.

Orientador: Durval de Paula Rodrigues Junior / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Agricola / Made available in DSpace on 2018-08-09T10:00:40Z (GMT). No. of bitstreams: 1 Padovani_VeraCristinaRamalho_M.pdf: 5126190 bytes, checksum: 92c46be09d3a5c6b107a74a1fbeab24e (MD5) Previous issue date: 2006 / Resumo: Este trabalho tem como objetivo estudar as características físicas e químicas de misturas de diferentes proporções de composto orgânico de lodo de esgoto com casca de arroz carbonizada e avaliar a viabilidade da utilização desses materiais como meio de crescimento para produção de mudas de essências nativas e exóticas. O experimento foi dividido em três fases nas quais foram realizados diversos ensaios. A primeira fase abrangeu a caracterização do lodo de esgoto, do material estruturante e do substrato comercial utilizado no viveiro através de análises químicas. A segunda fase envolveu a realização do processo de compostagem do lodo de esgoto utilizando-se restos de capim e grama cortados, como agente estruturante . Após a compostagem, o lodo de esgoto passou a receber a denominação de COLE ( composto orgânico de lodo de esgoto ) e foi submetido a nova análise química , microbiológica, parasitológica , além da condutividade elétrica. Posteriormente foi misturado com a casca de arroz carbonizada (CAC) nas proporções de 100/00 a 00/100 de COLE/CAC estabelecendo as misturas ou tratamentos que foram submetidos a análises para determinação das propriedades físicas, como macroporosidade, microporosidade, porosidade total , capacidade de retenção de água, densidade aparente . A terceira fase foi dividida em dois ensaios, sendo o primeiro um teste preliminar que utilizou o composto orgânico de lodo de esgoto para a semeadura e cultivo de uma planta indicadora , o tomate, que forneceu resultados que determinaram os tratamentos (substratos) para a instalação do ensaio final , que teve como objetivo o estudo do efeito do substrato na produção de mudas. O segundo ensaio compreendeu a avaliação da produção de essências nativas e exóticas e foi desenvolvido no viveiro municipal da Faculdade Municipal Professor Franco Montoro/Moji Guaçu, onde foram testadas como substrato as proporções 100/00, 90/10,80/20, 70/30, 60/40 (composto orgânico de lodo de esgoto /casca de arroz carbonizada ) utilizando-se tubetes , os quais foram comparados aos substratos testemunhas Plantmax da Eucatex e esterco de curral curtido utilizado pelo viveiro. Foram avaliados parâmetros relativos ao crescimento das mudas: altura de planta (H); diâmetro de colo (D); relação H/D; produção de matéria seca de parte aérea e raiz; relação matéria seca de raiz/matéria seca de parte aérea , área foliar e qualidade de torrão. Os resultados obtidos quanto à caracterização física, microbiológica e química do composto orgânico de lodo de esgoto ( COLE) , indicam que o material está adequado para ser utilizado como meio de propagação de espécies florestais. As mudas de árvores nativas Ingá, Mirindiba rosa e Coração de negro desenvolvidas em substratos contendo composto orgânico de lodo de esgoto ( COLE) / casca de arroz carbonizada (CAC) nas proporções 90/10 e 80/20, apresentaram resultados satisfatórios quando comparados às testemunhas. A espécie exótica Ipê de jardim apresentou bons resultados de crescimento e desenvolvimento das mudas na proporção 90%COLE /10%CAC . Com relação a caracterização nutricional das mudas de Ingá, observou-se que os tratamentos contendo doses de COLE de 70 a 100% apresentaram maior acúmulo de nutrientes nas folhas. A dose de 100% de COLE apresentou resultados superiores em crescimento da parte aérea das mudas, mas insatisfatórios no desenvolvimento das raízes, dificultando a retirada da muda do tubete e interferindo na qualidade final da muda. Com base nos resultados obtidos, conclui-se que o uso do composto orgânico de lodo de esgoto para produção de mudas em tubetes é bastante promissor desde que ele seja utilizado em composição com outro material estruturante , como por exemplo a casca de arroz carbonizada, a fim de se obterem mudas com melhores qualidades fisiológicas e morfológicas / Abstract: This work has as objective to study the physical and chemical characteristics of mixtures of different ratios of organic mixture of sewage with carbonized rice straw and to evaluate the viability of the use of these materials as medium of growth for production of seedlings of native and exotic essences. The experiment was divided in three phases where diverse assays had been carried through. The first phase enclosed the characterization of the sewage sludge, the structural material, and the used commercial substratum in the fishery, through chemical analyses. The second phase involved the accomplishment of the process of composting of the sewage sludge using itself cut remaining portions of grass and gram, as structural agent. After the composting of the sewage sludge to be called COLE ( organic mixture of sewage sludge) and was subdue the new chemical analyses , chemical of microorganism and electric conductivity. Subsequently the sewage sludge and mixes the carbonized rice straw, had been carried through analyses for determination of the physical properties, as macroporosity, microporosity, total porosity, water retention capacity , apparent density and electric conductivity. The third phase was divided in two assays, having been first a preliminary test that used the organic mixture of sewage sludge for the sowing and culture of an indicating plant, the tomato, that it supplied resulted that they had determined the treatments (substratum) for the installation of the final assay, that had as objective the study of the effect of the substratum in the production of seedlings. As the second assay understood the evaluation of the production of native and exotic essences and was developed in the municipal fishery of the Municipal College Professor Franco Montoro /Mogi Guaçu, where ratios had been tested as substratum, 100/00 90/10,80/20, 70/30, 60/40 (organic mixture of sewage sludge/ carbonized rice straw) using tubetes, which had been compared with substrate witnesses Plantmax of the Eucatex and dung of tanned corral used for the fishery. Relative parameters to the growth of the changes had been evaluated: height of plant (h); collar diameter (d); relation H/D; dry matter production of aerial part and root; ratio between dry matter of root and aerial part; leaf area and quality of aggregation of the substratum. The gotten results how much the physical, microbiological and chemical characterization, of the organic mixture of sewage sludge (COLE), indicates that the material is adjusted to be used as half of propagation of forest species. The changes of native trees Ingá, Mirindiba rosa and Coração de negro in substrata I contend organic mixture of sewage sludge (COLE)/ carbonized rice straw (CAC) in ratios 90/10 and 80/20, had presented resulted satisfactory when comparative the witnesses. The exotic species Ipê de jardim presented good results of growth and development of the seedlings in the ratio 90%COLE /10 %CAC. With regard to nutrition characterization of the seedlings of Ingá, it was observed that the treatments I contend doses of organic mixture of sewage sludge (COLE) of 70 the 100%, had presented greater accumulation of nutrients in leaf. The dose of 100% of organic mixture of sewage sludge (COLE) presented resulted superior in growth of the aerial part of the seedlings, but unsatisfactory in the development of the roots, making it difficult the withdrawal of the dumb one of container and intervening with the final quality of the seedling . On the basis of the gotten results, the use of the organic mixture of sewage sludge for production of seedlings in containers is concluded that, is sufficiently promising since that, the same either used in composition with another structural agent , as for example the carbonized rind of rice, in order to get seedlings with better physiological and morphologic qualities / Mestrado / Agua e Solo / Mestre em Engenharia Agrícola

Western hemlock (Tsuga heterophylla (Raf.) Sarg.) and western red cedar (Thuja plicata Donn) seedlings from four dormancy induction treatment(s) (DIT) (i.e. long-day dry, long-day wet, short-day dry, and short-day wet) were planted on a high available soil moisture field site in British Columbia and monitored for physiological response and morphological development over the first growing season. Stomatal conductance (gwv) and net photosynthesis (Pn) were recorded over an environmental matrix of photosynthetically active radiation (PAR) (0 - 2.2 mmol m⁻² s⁻¹ ) and vapour pressure deficit (VPD) (0 - 2.5 or 4.0 kPa) on both first year grown and fully developed second year foliage. To compare stocktypes, physiological data were collected and analyzed in the following ways: (1) replicated data at stable environmental conditions once a month, (2) physiological response to one increasing environmental variable using boundary line analysis, and (3) physiological response surface to two simultaneously changing environmental variables. For western hemlock first year needles, short-day DIT had a higher gwv response to both increasing VPD and PAR. Both short-day and moisture stressed DIT improved Pn response to PAR, and the combination had the highest response. Short-day DIT seedlings initially were smaller, shorter, had a better seedling water balance ratio and lower shoot to root ratio. Short-day second year needles showed a slightly higher gwv response to both increasing VPD and PAR as compared to long-day seedlings; however, they showed no treatment differences for Pn versus PAR. All stocktypes had similar final morphological parameter values. For western red cedar first year foliage, moisture stressed DIT seedlings had greater Pn response to increasing PAR and VPD. Also, moisture stressed seedlings gwv response to increasing VPD and PAR was higher when compared to its' non moisture stressed daylength counterpart. The long-day wet seedlings initially had a larger root and shoot system as compared to the other stocktypes. Second year foliage exhibited no treatment differences for gwv and Pn response to PAR ' and VPD. All stocktypes had similar final morphological parameter values. Attempts to test stocktypes at stable environmental conditions proved, at times, difficult. Potentially large measurement variation due to atmospheric environmental changes, and not stocktype effect, could result. Boundary line analysis, when used correctly and with sufficient data, offers a good stocktype assessment method which enables the isolation of a physiological response to one environmental variable. Three dimensional surface response technique was required to provide a clear conceptual representation of two primary environmental variables' influence on seedling physiological response. / Forestry, Faculty of / Graduate

Rhizoctonia solani and Pythium spp. are aggressive soil-borne fungal pathogens responsible for seed rot and seedling damping-off of many crops. With increased environmental and public concern over the use of chemicals, biological control of these diseases has been attracting more attention. However, success with this strategy depends on the development of effective antagonists, which requires repeated in vitro and in vivo tests. Bacillus spp. were isolated from a soil sample obtained from a field where sorghum and tef had been grown for at least two years. Potential Bacillus isolates were screened for their ability to inhibit in vitro growth of R. solani and Pythium sp. Among 80 isolates tested, endospore forming Bacillus spp. H44 and H51 gave highest antifungal activity against the two test-pathogens in three consecutive tests. Results demonstrated that both H44 and H51 have potential as biocontrol agents against diseases caused by these two pathogenic fungi. The interaction between three isolates of Trichoderma (T. harzianum Eco-T, Trichoderma spp. SY3 and SY4) and Pythium sp. were investigated using in vitro bioassays together with environmental scanning electron microscopy (ESEM). Visual observation on the dual culture tests revealed that hyphal growth of Pythium was inhibited by these antagonists soon after contact between the two organisms within 3-4 days of incubation. The ESEM investigations showed that all three isolates of Trichoderma grew toward the pathogen, attached firmly, coiled around and penetrated the hyphae of the pathogen, leading to the collapse and disintegration of the host's cell wall. Degradation of the host cell wall was postulated as being due to the production of lytic enzymes. Based on these observations, antibiosis (only by Eco-T) and mycoparasitism (by all three isolates) were the mechanisms of action by which in vitro growth of Pythium sp. was suppressed by these Trichoderma isolates. The reduction of seedling diseases caused by R. solani and a pythium sp. were evaluated by applying the antagonists as seed coating and drenching antagonistic Bacillus spp. (B81, H44 and H51) and Trichoderma (T. harzianum Eco-T and Trichoderma spp. SY3 and SY4). On both crops, R. solani and Pythium sp. affected stand and growth of seedlings severely. With the exceptions of H51, applications all of isoltes to seeds reduced damping-off caused by R. solani in both crops. Application of Eco-T, H44 and SY3 to sorghum controlled R. solani and Pythium sp. effectively by yielding similar results to that of Previcur®. On tef, biological treatments with Eco-T and SY4 reduced seedling damping-off caused by R. solani and Pythium sp., respectively, by providing seedling results similar to the standard fungicides, Benlate® and Previcur®. Most other treatments gave substantial control of the two pathogens on tef. Overall, Bacillus sp. H44 and T harzianum Eco-T were the best biocontrol agents from their respective groups in reducing damping-off by the two pathogens. In all instances, effects of application method on performance of biocontrol agents and adhesive on emergence and growth of seedlings were not significant. A field trial was conducted at Ukulinga Research Farm at the University of Natal, Pietermaritzburg, South Africa, to determine efficacy of biological and chemical treatments on growth promotion and reduction of damping-off incited by R. solani and Pythium sp., and to evaluate the effects of a seed coating material, carboxymethyl cellulose (CMC), on seedling emergence and disease incidence. Seeds of sorghum and tef were treated with suspensions of antagonistic Bacillus H44 or T harzianum Eco-T, or sprayed with fungicides, Benlate® or Previcur®. Application of Benlate® and Previcur® during planting significantly increased the final stand and growth of sorghum seedlings. Seed treatments with both H44 and Eco-T substantially controlled damping-off caused by Pythium, resulting in greater dry weights of seedlings than the standard fungicide. However, they had negative effects when they were tested for their growth stimulation and control of R. solani. The CMC had no significant effect on germination and disease levels. These results showed that these antagonists can be used as biocontrol agents against Pythium sp. However, repeated trials and better understanding of the interactions among the antagonists, the pathogens, the crop and their environment are needed to enhance control efficiency and growth promotion of these antagonists. Some of these biocontrol agents used in this study have the potential to diseases caused by R. solani and Pythium sp. However, a thorough understanding of the host, pathogen, the antagonist and the environment and the interactions among each other is needed for successful disease control using these antagonists. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2003.

Thesis (PhD (Forest and Wood Science))—University of Stellenbosch, 2006. / Experiments carried out in this study sought to contribute to the understanding of the ecological interactions involved in Uapaca kirkiana seedling establishment and growth in a natural woodland ecosystem. These include soil pH reactions, plant root surface pH change, root exudates that affect the chemical behaviour of the soil in the vicinity of the root, and microbial effects. Although it was difficult to determine which of these factors play a dominant role in the soil-plant relationship, the study contributed to the understanding of the mycorrhizal fungi-host plant association. It revealed the diversity of mycorrhizal fungal species occurring in the different ecological sites, and also analysed the relationship between soil factors. Soil pH and K had significant influences on the fungi population diversity. K was considered important in its role in the translocation of auxins to the root sites where they enhance root susceptibility to mycorrhizal fungal infection. More work needs to be undertaken to establish the role of soil pH.

The aim of the study was to determine the effect of day/night temperatures (22/l6°C, 2712l oC and 33/27°C) and phosphorus levels (0, 0.12 and 1.2g per 20 kg soil) on seedling establishment and seed viability during three stages of seed development (15, 22 and 33 days after flowering) for seed of normal and quality protein maize cultivars. Soluble carbohydrate accumulation and mineral element content were determined using environmental scanning electron microscopy (ESEM) in relation to seed phytate levels and seed germination capacity at different stages of development. Leaf emergence rate and plant height during seed development were significantly (P < 0.05) influenced by temperature and phosphorus nutrition. Phosphorus in seed is stored primarily in the form of phytic acid, also known as phytate. Accumulation of phytate takes place during maturation phase of seed development. Phosphorus nutrition and temperature also caused a.significant (P < 0.05) increase in seed germination at all stages of seed development. Furthermore, phosphorus nutrition and temperature influenced occurrence of soluble carbohydrates in seeds. Myo-inositol, the sugar alcohol that forms the basic structure of phytate, was increased by P nutrition and increasing growth temperature. Whereas, QPM maize was generally found to perform poorly than normal maize, with respect to phytate content, seed germination and seedling establishment, both cultivars displayed the same responses to phosphorus nutrition and temperature. In both cultivars, globoids, the sites of phytate synthesis and storage, were found only in the embryonic axis. Subsequently, there were significantly low levels of mineral elements (P, Mg and K) found in the endosperm, compared with embryonic axis. This finding suggested that the embryonic axis plays a major role in seed performance, through its effects emanating from phyate metabolism. Myo-inositol plays a role in membrane biogenesis during stress conditions such as temperature by maintaining the integrity of the cell wall and minimizes the leaching of cations essential during germination. Myo-inositol, although it occurs in small concentrations, could be used to indicate seed quality in maize, because its accumulation was found to be associated with enhanced phyate levels and better seed germination in a wide range of temperatures. Low vigour seeds are associated with high electrolyte leakage during imbibition. Mineral elements form a significant portion of the imbibition leachate, which causes seeds to lose nutrients for early seedling growth. This study provided evidence that phosphorus nutrition can alleviate poor seed vigour of maize by improving phytate levels. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.