This thesis reports the results of an investigation into the factors affecting the fertilising ability of avian germ plasm. The study was limited to males of a Rhode Island Red control population and females of a commercial white egg-laying hybrid. The stock was replaced annually from the same source. The investigation was broadly organised according to three major objectives: 1) Development of an objective in vitro test to predict fertilising ability.2) A study of some management and biological factors affecting fertilising ability of semen.3) Development of a diluent and conditions for extended storage of fowl semen at high temperatures of 20 and 40?C. A simple, objective, controlled colourimetric technique was developed for estimating the capacity of fowl spermatozoa to reduce a tetrazolium dye to a coloured compound, formazan, which could be measured spectrophotometrically. The reduction of this dye is dependent on number of spermatozoa, incubation temperature and time of incubation. The test is shown to give a quantitative measurement of the metabolic activity of spermatozoa, as judged by their rate of oxygen utilisation, and thus of the quality of semen. The rate of tetrazolium dye reduction was shown to be highly correlated with sperm motility, morphology, ATP content and fertilising ability. The method has many practical advantages over existing tests for the prediction of fertilising ability. A study of frequency of semen collection, over a period of 4 weeks, showed that the fertilising ability was not significantly different when semen was collected twice a day, once a day or thrice a week. Exchange of seminal plasma between samples of semen from males designated as highly or poorly fertile, or as young or old, did not influence the de novo fertilising ability of the original spermatozoal samples. The investigation of the age of male and female revealed that age of male influenced fertilising ability but not the age of females. However, interaction in fertilising ability between age of male and age of female was significant. A simple diluent and storage condition was developed for holding fowl semen at high temperatures of 20? and 40?C for 17 hr. A simple method for storing semen for 6 hr in a still condition on a bench top around 20?C, which should be suitable for field situations, is described. This thesis examines some features of fowl semen physiology concerned with identifying quality of spermatozoa, and some beneficial procedures for the better exploitation of artificial insemination of probable benefit to breeders of commercial poultry.

No description available.

Made available in DSpace on 2014-06-11T19:35:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-03-28Bitstream added on 2014-06-13T18:46:30Z : No. of bitstreams: 1 carreira_jt_dr_jabo.pdf: 630709 bytes, checksum: f21215f6ef9c8182c2586466e4629742 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Fundação para o Desenvolvimento da UNESP (FUNDUNESP) / Os objetivos deste projeto de pesquisa foram testar se a qualidade espermática, e a integridade da cromatina são diferentes em um grupo de touros Nelore jovens, adultos e senis. Três ejaculados, de 40 touros Nelore foram subdivididos em três faixas etárias: de 1,8 a 2 anos – Grupo Jovens, de 3,5 a 7 anos – Grupo Adultos e de 8 a 14,3 anos – Grupo Senis. As amostras foram avaliadas quanto à motilidade, o vigor, teste de termoresistência lento (TTL), análise computadorizada do movimento espermático (CASA), avaliação simultânea da integridade da membrana plasmática e do acrossoma, potencial mitocondrial, protaminação espermática, danos de DNA causados por radicais livres, suscetibilidade a denaturação da cromatina, pelos testes “Sperm Cromatin Structure Assay” (SCSA) e laranja de acridina (AOT), e danos na cromatina com a técnica TUNEL. Os animais jovens apresentaram valores superiores de motilidade e vigor antes e após o TTL, e maior porcentagem de células com alterações morfológicas, assim como maior porcentagem de espermatozóides com membrana e acrossoma íntegros e com alto potencial mitocondrial, estes parâmetros tiveram os menores índices nos animais senis (P<0,05). Nas avaliações da integridade do DNA, os touros jovens apresentaram maior porcentagem de deficiência de protaminação, enquanto os senis danos por oxidação. No teste SCSA os touros mais jovens e mais idosos apresentaram maiores índices de danos na cromatina, diferindo dos demais (P<0,05). O teste de TUNEL não apresentou resultados satisfatórios. Estes resultados permitem concluir que touros jovens apresentam qualidade espermática superior, seguidos pelo grupo de animais adultos, e finalmente um declínio na qualidade nos diversos parâmetros estudados para o grupo de animais senis. Quanto à integridade... (Complete abstract click electronic access below) / The aims of this study were to test if sperm quality and chromatin integrity are different in young, adult and senile Nelore Bull cryopreserved semen samples. Three ejaculates from 40 Nelore bulls were divided by age in three groups: 1,8 to 2 years – Young Bulls, from 3,5 to 7 years – Adult Bulls and from 8 to 14,3 years – Senile Bulls. Samples were evaluated for: motility, vigor, slow termoresistance test (TTL), computadorized sperm movment analyses (CASA), simultaneous evaluation of plasmatic membrane and acrosome integrity, mitochondrial potential, sperm protamination, DNA oxidation damages, Sperm Cromatin Structure Assay (SCSA) and acridine orange test (AOT), and TUNEL. Young animals presented better values for motility and vigor before and after TTR , intact membrane and acrosome and high mitochondrial potential, but higher values for sperm defects, (P<0.05). On DNA integrity evaluations, Young Bulls presented more protamination problems while senile bulls guanine oxidation defects, both groups were more susceptible to DNA denaturation on SCSA (P<0.05). TUNEL results were not satisfatory. The results indicates that Young bulls may have a high quality sperm sample after freezing, followed by the adults and finally there is a decrease in quality on senile bulls group, nevertheless the results would probably not compromised the samples approval for commercial use. Regarding DNA integrity, Young and senile bulls are more susceptible to DNA damage, nevertheless the etiology of the damage seems to be different, by protamine deficiency in Young bulls and by oxidation in senile animals

Made available in DSpace on 2014-06-11T19:29:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2003Bitstream added on 2014-06-13T20:38:44Z : No. of bitstreams: 1 medeiros_asl_me_botfmvz.pdf: 309351 bytes, checksum: 0a3f78bcf756083f2e3741923096ea7b (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / The present work aimed to investigated several kinds of amides to freeze equine semen. Two ejaculates from 20 stallions of different breeds were used. All ejaculates with initial motility greater than 60 % were split and frozen in 0.5 ml French straws in a milk base extender (INRA 82) containing the different studied cryoprotectants. The post-thaw percentage of motility and other motility parameters wer evaluated using CASA .To evaluate cell viability fluorescent probes were used. Stains used were Carboxyfluorescein and propidium iodine (PI). The first experiment was designed to investigated the protective effect of 3 amides ; Dymethyl acetamide at 3 concentrations - 1, 3 and 5 % v/v; dymethyl formamide at 5% and methyl formamide at 5%. Differences on motion parameters between cryoprotectant treatments were determined by analysis of variance using SAS system. Utilization of amides significantly improved most of parameters evaluated when compared with Glycerol. Progressive motility evaluated by optical microscopy was higher (p< 0,0001) for semen that was frozen with MF 5% and DF5%. The progressive motility evaluated by CASA was higher for the MF 5 % and DA 3% groups. The electron microscopy evaluation showed that DA 5% and MF 5 % were superior in the ability to preserved the acrossomal integrity. The acrossomal status evaluated by fluorescence showed that the preservation was higher on the groups DA 5%, MF 5 % and DF5% (p<0.0001). On the second experiment the cryoprotective effect of two associations of glycerol (Gly) and dimethylformamide (DF) were tested ( Gly 3.5% + DF 1.5 % and DF 3.5% + Gly 1.5%)... (Complete abstract, click electronic address below).

Made available in DSpace on 2014-06-11T19:29:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2003Bitstream added on 2014-06-13T19:38:33Z : No. of bitstreams: 1 chirinea_vh_me_botfmvz.pdf: 882967 bytes, checksum: dbe4419b2561f045402e0da0d59ad8da (MD5) / Fundação para o Desenvolvimento da UNESP (FUNDUNESP) / Com o objetivo de se avaliar dois meios diluidores - Tris/Glicerol e MP50 - para a congelação do sêmen de cães, foram utilizados ejaculados de 10 cães adultos, colhidos por meio de manipulação digital. As características morfofuncionais foram analisadas no sêmen in natura (T1), refrigerado (T2) e descongelado (T3). As amostras foram avaliadas para: motilidade e vigor espermático, teste hiposmótico, integridade de membrana espermática, morfologia espermática e análise ultra-estrutural, além da mensuração do pH. Após a diluição com os diferentes meios, o sêmen foi envasado em palhetas francesas de 0,5mL, contendo 40 x 106 espermatozóides móveis/palheta, e mantidas por 60 minutos à 5ºC (T2); logo após, foram transferidos para o vapor de nitrogênio durante 20 minutos, e por fim, mergulhadas em nitrogênio. O armazenamento das palhetas foi em botijão criogênico. As palhetas foram descongeladas a 70ºC por 8 segundos. A análise de variância mostrou influência do efeito animal nas variáveis analisadas, com exceção da motilidade espermática e integridade de membrana espermática. O teste hiposmótico, a integridade de membrana espermática e o pH, nas amostras refrigeradas (T2), apresentaram diferença estatística significativa (p<0,05) entre os meios, com superioridade do MP50. Nas análises pós-descongelação (T3), não foram observadas diferenças significativas entre as variáveis estudadas para os dois meios. A análise ultra-estrutural, mostrou edema de membrana plasmática e acrossomal, nas diferentes etapas do processo de congelação. Em conclusão, considerando-se as características morfofuncionais do sêmen canino pós-descongelação, os meios diluidores demonstraram igualdade. / The objective of this study was to verify the efficiency of two different extenders for freezing dog semen: Tris/Glycerol and MP50. Ten ejaculates from different adult dogs were collected by digital manipulation. Seminal characteristics were evaluated in three different moments, fresh (T1), cooled (T2) and thawed (T3) semen for sperm motility and vigor, hypo-osmotic swelling test, sperm membrane integrity, sperm morphology, ultrastructural analysis and seminal pH. The samples were divided into two equal parts and each part was mixed with one extender type. After mixing, samples were packaged in 0,5mL French straws with 40 x 106 spermatozoa/straw. Semen samples were kept at 50C for 60 minutes (T2); then frozen in static vapor of nitrogen for the following 20 minutes and immersed in liquid nitrogen until being thawed in 700 C water for 8 seconds (T3). By analysis of variance, it would be possible to verify the animal effect on almost all variables observed in this study, except for sperm motility and membrane integrity. For cooled semen (T2), MP50 were significantly better for hypo-osmotic swelling test, sperm membrane integrity and pH (p<0,05) than Tris/Glycerol. For thawed semen (T3), there was no significant difference between extenders. By ultrastructural analysis, it was possible to verify swelling plasma and acrosomal sperm membranes in the different stages of freezing process. In conclusion, the extenders showed the same results as to morphofunctional characteristics the semen canine thawed.

Made available in DSpace on 2014-06-11T19:29:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-29Bitstream added on 2014-06-13T20:20:21Z : No. of bitstreams: 1 silva_lfi_me_botfm.pdf: 366320 bytes, checksum: b20723bbb3bc87f0ad3fe980c63f1375 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Poucos estudos avaliando o efeito da idade paterna nos resultados de RA tem sido descritos na literatura. A idade avançada do homem parece influenciar na função reprodutiva masculina em menor grau quando comparada com a idade da mulher, pelo simples fato da ocorrência de nascimentos de crianças de pais com idade entre 70 e 94 anos. O efeito da idade paterna na qualidade do sêmen tem sido discutido na literatura porém os resultados são contraditórios. Frente a tais contradições, este artigo de revisão tem como objetivo descrever e discutir os dados da literatura no que diz respeito a influência da idade paterna nos parâmetros seminais (volume, concentração, motilidade e morfologia) , nos danos do DNA espermático , nos resultados de rerpodução assistida (fertilização, desenvolvimento embrionário, implantação, gravidez, aborto e nascimentos) e em doenças genéticas / Few studies evaluating the effect of paternal age on the results of ART has been described in the literature. Advanced paternal age seems to influence the male reproductive function to a lesser degree than the female’s, as evidenced by the fact that babies have been fathered by aged 70 to 94 years. The effect of paternal age on semen quality has been discussed in the literature but the results are not consistent. Front these contradictions this review article aims to describe and discuss the literature data on the influence of paternal age on the semen parameters (volume, concentration, motility and morphology), sperm DNA damage, outcomes of ART (fertilization, embryo development, implantation, pregnancy, abortion and birth) and genetic disease

Orientador: Aloisio Jose Bedone / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas / Made available in DSpace on 2018-07-21T17:15:55Z (GMT). No. of bitstreams: 1 Sousa_LourivaldoRodriguesde_D.pdf: 2842913 bytes, checksum: 2bb70d08cfbf4cfd1f12f95f12dfc7f4 (MD5) Previous issue date: 1996 / Resumo: Com o objetivo de avaliar os resultados gestacionais de um programa de Inseminação Artificial com Sêmen de Doador (IAD), foi realizado um estudo clínico observacional com 480 casais no Instituto Valenciano de Infertilidade (IV!) - Espanha, utilizando diferentes esquemas' de estimulação ovariana ou não, no período de janeiro de 1990 a dezembro de 1994. Esses casais submeteram-se a 1429 ciclos de inseminações intra¬ uterina (IIU), sendo que 235 eram pacientes cujos maridos apresentavam diagnóstico de azoospermia e 245 mulheres cujos maridos apresentavam esterilidade masculina de causa severa. O tempo médio de esterilidade foi de 4,6 anos e a idade média das mulheres foi de 31,8 anos. Para análise dos dados foi utilizado o modelo estatístico de Kaplan-Meier, sendo considerados significativos valores de P < 0,05. "Foram obtidas um total de 253 gestações, com uma taxa de gestação por paciente de 52,7% e uma taxa acumulada de gestação em seis ciclos de 67,1%. Observamos resultados gestacionais significativamente melhores no grupo com estimulação ovariana (notadamente no grupo com FSHhp) quando comparados com o grupo sem estimulação. Os resultados gestacionais obtidos após três e seis ciclos de inseminações foram muito semelhantes. Os resultados.gestacionais foram melhores no grupo de pacientes azoospérmicos apenas nos ciclos naturais. Não houve influência do fator idade nos resultados analisados. Concluímos que a taxa de gestação por paciente e a taxa acumulada de gestação foram superiores no grupo cuja ovulação fora estimulada com FSHhp e que os resultados gestacionais obtidos com três e seis ciclos não foram diferentes / Abstract: In order to evaluate the outcome pregnancy rate of the IAD program were performed a observationaI c1inic study on Institut Valenciano de InfertiIidad- Valencia - Spain ITom 1990 to 1994. 480 infertiIes coupIes had 1429 cyc1es of IUI inseminations resulting 253 pregnancies. Among this coupIes, 235 were pacients whose husbands presented azoospermia and 245 coupIes whose husbands presented infertiIity of severe male cause. The couples presented and average history of infertility of 4.6 :t 1.1 years. The women average was 31.8 :t 4.2 years oId. For this anaIysis we used the Statical model ITom Kaplan-Meier, using P < 0,05.The pregnancy rate per patient were 52.7% and the accumulated rate ofpregnancy in6 cyc1es of 67,1 %. We observed pregnancy rate significative higher in the ovarian stimulation (namely in the FSHhp group) who compared with natural cyc1es. Similar outcome pregnancy rate were obtained after 3 and 6 inseminations cyc1es. We observed the differences between the results obtained in couples with azoosperm partners and with oligoasthenosperm partners just in the natural cycles group. The age factor had no influence in the results.We conc1uded that the 111 pregnancy rate were superior in the group that received ovarian stimulation rather than the natural cycles, obtained with FSHhp higher pregnancy rate and, similar rates pregnancy with 3 and 6 cycles were observed. / Doutorado / Tocoginecologia / Doutor em Medicina

Orientador: Mary Anne Heidi Dolder / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-03T17:20:21Z (GMT). No. of bitstreams: 1 Mancini_KarinaCarvalho_D.pdf: 22242865 bytes, checksum: 011013b83d6b4da00716d61fef0eeb42 (MD5) Previous issue date: 2003 / Resumo: Borboletas e mariposas apresentam um dos casos mais evidentes de polimorfismo espermático, com a produção de dois tipos de espermatozóides: os apirenes e os eupirenes, que diferem em morfologia e função. Estudos ultra-estruturais descreveram a morfologia e organização de ambos os tipos de espermatozóides ao longo dos tratos reprodutores masculino e feminino da borboleta Euptoieta hegesia. Os espermatozóides apirenes extratesticulares adquirem membranas concêntricas externas provenientes do rearranjo da membrana plasmática. Já os eupirenes, adquirem um complexo envoltório que sofre modificações ao longo dos tratos reprodutores e parece ser parcialmente resultante do rearranjo dos apêndices laciniados. Foram usados também métodos citoquímicos, à saber: ácido fosfotúngstico-etanólico, ácido tânico, cuprolinic blue, vermelho de rutênio, tiosemicarbazida/proteinato de prata e lectinas. Apirenes e eupirenes apresentaram, diferencialmente, proteínas e carboidratos no lúmen dos microtúbulos e nos elementos de ligação do axonema, nas membranas e principalmente nas estruturas extra-celulares. Nos espermatozóides apirenes ainda foram detectados esses componentes no capuz anterior e nas regiões paracristalinas dos derivados mitocondriais. Nos espermatozóides eupirenes, os apêndices laciniados apresentaram, principalmente, componentes protéicos, enquanto os apêndices reticulados apresentaram carboidratos. Ambos os tipos de apêndices apresentaram organização paracristalina, formada por estruturas cilíndricas. Além disso, por meio da técnica de ácido tânico, foi verificada significativa similaridade entre os envoltórios apirenes e eupirenes, indicando uma possível origem comum. Com o uso de lectinas, os apêndices laciniados e os envoltórios apirenes e eupirenes apresentaram os mesmos glicoconjugados, sugerindo que os envoltórios se originaram do rearranjo destes apêndices. O método imunocitoquímico para detecção de tubulinas mostrou que os apêndices laciniados não são compostos microtubulares. No trato reprodutor feminino foram descritas as morfologias da espermateca e dos espermatozóides apirenes e eupirenes armazenados. O epitélio espermatecal, desconhecido na literatura, apresenta morfologia similar àquela encontrada em outras ordens de insetos / Abstract: Butterflies and moths present one of the most evident examples of sperm polymorphism, with the production of two types of spermatozoa: the apyrene and the eupyrene, that differ in functional and morphological characteristics. Ultrastructural studies were carried out to describe the morphology and organization of both sperm types along the male and female reproductive tracts of the butterfly Euptoieta hegesia. The extra-testicular apyrene spermatozoa acquire external concentric membranes as a result of the plasma membrane rearrangement. The eupyrene spermatozoa acquire a complex coat that is modified along the reproductive tracts and is apparently originates from the rearrangement of the lacinate appendages. Different cytochemical methods were applied: ethanol fosfotungstic acid, tannic acid, cuprolinic blue, ruthenium red, tiosemicarbazide/silver proteinate and lectins. Both sperm types presented differences in the proteins and carbohydrates found in the microtubule lumens and in the links binding the axoneme, in the cellular membranes and, principally, in the extracelullar structures. In apyrene sperm these components were detected in the anterior cap and in the paracrystalline cores of the mitochondrial derivatives. In the eupyrene sperm, the lacinate appendages were predominant1y protein in composition, while the reticular appendages seem to be composed principa11y of carbohydrates. Both appendage types presented paracrystalline organization, made up of small cylindrical structures. With the tannic acid technique, a significant similarity was verified between the coats of both sperm types, indicating a possible common origin. With t he lacinate technique, the lacinate appendages and the c oat of both sperm types were shown to contain the same glycoconjugates, suggesting that the coats are originated by reorganization of these appendages. The immunocytochemical method for tubulin detection demonstrated that the lacinate appendages are not microtubular structures. In the female reproductive tract, the morphology of the spermatheca was described as well as the apyrene and eupyrene spermatozoa, stored in this organ after mating. The morphology of the spermatheca epithelium, which had not been previously investigated for Lepidoptera, presented a somewhat similar organization to what is known for other insect / Doutorado / Biologia Celular / Doutor em Ciências Biológicas

Orientador: Mary Anne Heidi Dolder / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-03T17:19:44Z (GMT). No. of bitstreams: 1 Zama_UyradosSantos_D.pdf: 14815313 bytes, checksum: e7f1b0ffc689c69a7c9c5871c7f60bc4 (MD5) Previous issue date: 2003 / Resumo: O termo "espermiocladística" defini a utilização de características estruturais e ultra-estruturais dos espermatozóides na composição de matrizes de caracteres para estudos filogenéticos. Estudos realizados, inclusive com insetos, têm fornecido dados consistentes e úteis para este fim, pelo menos para táxons superiores. Neste sentido, propusemos para este trabalho de tese, caracterizar detalhadamente a morfologia dos espermatozóides nas tribos Meliponini, Euglossini e Bombini, buscando identificar caracteres potenciais que pudessem contribuir para o entendimento das relações filogenéticas do grupo. Para tanto, utilizamos as metodologias usuais de (1) microscopia de luz, contraste de fase e fluorescência com DAPI e (2) Microscopia Eletrônica de Transmissão, convencional e citoquímica com ácido tânico e E-PTA. Ainda, comparamos nossos dados com o descrito na literatura para a tribo Apini, para que pudéssemos compor uma matriz consistente de caracteres. Nossas análises filogenéticas, embora incipientes, sugerem (Meliponini (Euglossini (Bombini + Apini))). Entretanto, acreditamos que nossos dados devam ser associados aos dados de morfologia somática, comportamento e biologia molecular, para que as análises filogenéticas futuras sejam mais conclusivas. Este trabalho originou 2 manuscritos em fase de submissão, um já aceito para publicação e um artigo já publicado: Zama, U.. Uno-Neto, J. & Dolder, H. 2001 . Ultrastructure of spermatozoa in Plebeia (Plebeia) droryana Friese (Hymenoptera: Apidae: Meliponina). Journal of Hymenoptera Research, 10 (2): 261-270 / Abstract: The term "spermiocladistics" express the use of structural and ultrastructural charcteristics to compose character matrices for phylogenetic studies. Such studies, carried out for different animais, including insects, have been shown to be consistent and useful, at least for higher taxa. Therefore, we proposed, in this thesis, to carry out a detailed ultrastructural analysis of the spermatozoa of the tribes: Melliponini, Euglossini and Bombini, in order to identify characters that could potentially contribute to the understanding of phylogenetic relationships in this group. For this study, we employed the methods: (1) light microcopy using phase contrast, DAPI fluorescence and (2) transmission electron microscopy with conventional preparations and cytochemistry (EPT A). Our data were compared with those described in the literature for the Apini tribe, so as to compose a consistent character matrix. Phylogenetic analysis of our data suggested (Meliponini (Euglossini (Bombini + Apini»). However, we believe that our data should be considered together with the data obtained with research on somatic morphology, behavior and molecular biology, in an effort to develop more consistent results. This thesis includes two manuscripts that are being submitted for publication, one was accepted and one article already published: : Zama, U., Uno-Neto, J. & Dolder, H. 2001. Ultrastructure of spermatozoa in P/ebeia (P/ebeia) droryana Friese (Hymenoptera: Apidae: Meliponina). Journal of Hymenoptera Research, 10 (2): 261-270 / Doutorado / Biologia Celular / Doutor em Biologia Celular e Estrutural

Semen contain a heterogeneous population of viable and non-viable (damaged) spermatozoa. Proportions of non-viable spermatozoa interfere with male fertility, with available techniques unable to selectively remove prior to breeding. Nanobiotechnology may allow removal, enriching semen with high quality spermatozoa for improved productivity. Here, we applied double nanopurification with boar semen using functionalized magnetic nanoparticles. Non-viable and viable spermatozoa were magnetically separated and verified through various microscopy imaging. Viable (nanopurified) spermatozoa showed no additional damages. Nanopurification did not interfere with sperm motility and viability, with beneficial effects on motion parameters. Nanopurified spermatozoa maintained fertility following insemination, with resulting offspring indicating no impaired growth or health performance. Pork quality was unaffected showing comparable characteristics to the control. In summary, the use of magnetic nanopurification in boar spermatozoa showed sperm viability and fertility improvements with successful offspring performance. This study shows promise for large-scale commercial applications to enhance male fertility and offspring performance.