Thesis (MSc)--Stellenbosch University, 1967. / ENGLISH ABSTRACT: no abstract available / AFRIKAANSE OPSOMMING: geen opsomming

Dressed, bagged whole chickens from three Oregon and several out-of-state processors were purchased from retail markets in each season in 1988. Birds were stored at 3°C for 6 days. Total aerobic microorganisms, total psychrotrophic microorganisms, pseudomonads and fluorescent pseudomonads were determined by appropriate procedures. Total aerobic microorganisms and psychrotrophic microorganisms were counted on standard plate count agar with incubation at 20°C for 3 days and at 5°C for 7 days, respectively. Two media, King's B medium and CFC medium, were used in counting pseudomonads. Fluorescent colonies were observed on King's medium under ultraviolet light. A simple slime smear test was used to determine the sliminess. Sensory evaluation was done by thirteen panelists using 9-point scales. The flavor of cooked white and dark meat and skin, the flavor intensity of cooked white and dark meat and skin, the aroma of raw and simmered meat, the aroma intensity of raw and simmered meat and raw sliminess were evaluated. Simple regression analysis was used to determine the relationships between the microbial parameters and sensory evaluations. The paired t test was used in determining the difference between counts on King's medium and CFC medium. A significance level of 95% was set for all tests. Correlation coefficients were also calculated. All the microbial counts were at or below 10⁷/cm², which indicated from literature comparisons that most of the fryers purchased from retail markets and stored for six days were of acceptable quality. The season had no significant effect on the microbial counts and sensory qualities. The means of flavor of cooked meat and skin and aroma of raw and simmered meat were all above fair. Only the raw aroma intensity was significantly (p<0.05) and strongly correlated (r=-0.88) to the aroma quality. Relationships between microbial counts and flavor of cooked meat and aroma of raw and simmered meat were all significant but the correlations were weak. The narrow range of microbial counts may explain the weakness of the correlations found. The slime smear tests had a positive relationship (p<0.05) to the raw sliminess score by panelists, total aerobic microorganisms, total psychrotrophic microorganisms, pseudomonads, and fluorescent pseudomonads. / Graduation date: 1990

Blackberries have been enjoyed for their delicious flavor and high nutritional value for hundreds of years. In North America, blackberries were cultivated since 1850-1860. Blackberries have their characteristic and unique aromas depending on the cultivar and growing conditions. The objective of this study was to evaluate the impact of seasonal variation and growing environment on blackberry aroma. Volatile compositions of 'Marion' and 'Thornless Evergreen' blackberries from three growing seasons were analyzed using gas chromatography-flame ionization detection (GC-FED) and GC-mass spectrometry (GC-MS). Seasonal variations were observed for some volatile compounds in these two blackberry cultivars. In 'Marion', the compounds were mainly acids, while in 'Thornless Evergreen', they were mainly acids, alcohols, and a few terpenoids. Although seasonal variations were present, it was generally observed that the most abundant volatiles in 'Marion' blackberry were acetic, 2/3-methylbutanoic, hexanoic and decanoic acids and linalool, whereas the most abundant volatiles in 'Thornless Evergreen' were 2-heptanol, hexanol, octanol, α-pinene, nopol and/p-cymen-8-ol. Compared with 'Marion', 'Thornless Evergreen' contained significantly more total volatiles, especially in alcohols, terpenoids and phenols, whereas 'Marion' contained more organic acids. Odor Activity Values (OAVs) were used to determine the most potent odorants in each cultivar. The compounds with high odor activity values (OAV > 10) in 'Marion' were ethyl hexanoate, β-ionone, linalool, 2-heptanone, 2-undecanone, α-ionone and hexanal. The compounds with high odor activity values (OAV > 10) in 'Thornless Evergreen' were ethyl hexanoate, 2-heptanone, ethyl 2-methylbutanoate, 2-heptanol, 3-methylbutanal, α-pinene, limonene, p-cymene, linalool, t-2-hexenal, myrtenol, hexanal, 2- methylbutanal and sabinene. To investigate the influence of growing environment on blackberry aroma, aroma extraction dilution analysis (AEDA) was employed to characterize the aroma profile of 'Chickasaw' blackberries from two growing regions in United States: Oregon and Arkansas. Eighty-seven odorants were detected in the two berries, in which seventy-seven could be identified. Comparative AEDA analysis showed that the berries grown in these two regions had similar aroma compositions, however, those odorants had various aroma impacts in each region. The compounds with high FD factors in Oregon's 'Chickasaw' were ethyl butanoate, linalool, methional, trans,cis-2,6-nonadienal, cis-l,5-octadien-3-one, and 2,5-dimethyl-4-hydroxy- 3(2H)-furanone, whereas in the 'Chickasaw' grown in Arkansas, they were ethyl butanoate, linalool, methional, ethyl 2-niethylbutanoate, β-damascenone and geraniol. The flavor formation in 'Chickasaw' blackberries was largely influenced by their growing environment. / Graduation date: 2005

Thesis (MTech (Food Technology))--Cape Peninsula University of Technology, 2018. / The aim of this study was to evaluate the physical, chemical and sensory characteristics of non-alcoholic pearl millet beverage produced using isolated and purified cultures of bioburden lactic acid bacteria (LAB). Traditional non-alcoholic pearl millet beverage (TNAPMB) was produced through spontaneous fermentation. The slurry was fermented for 36 h at 37°C while monitoring the microbial growth at 3 h interval. LAB were grown on deMan, Rogosa and Sharpe agar and identified using Vitek 2 system. The initial numbers of LAB were 7.04 log cfu/ml and increased to 8.00 log cfu/ml after 21 h. The beverage was dominated by LAB and contaminants and their survival was in succession. LAB from the genera Leuconostoc, Pediococcus, Streptococcus and Enterococcus were the main fermenting species in TNAPMB. Pearl millet extract (PME) was produced by hydrating pearl millet flour (PMF) with water (1:10, PMF:Water). To the mixture sprouted rice flour (10%), ground ginger (10%) and pectin (0.6%) were added. Stable PME was used in the production of plain non-alcoholic pearl millet beverage (PNAPMB). PME was pasteurized at 98°C for 30 min, hot filled and cooled to 25°C. The fluid was inoculated with Leuconostoc mesenteroides, Pediococcus pentosaceus and Enterococcus gallinarum each at 0.05, 0.075 and 0.1%, respectively, using factorial design and fermented for 18 h at 37°C. The pH of the beverage ranged between pH 3.32 and pH 3.90. L. mesenteroides, P. pentosaceus, E. gallinarum, the interaction between L. mesenteroides and P. pentosaceus and the interaction between L. mesentoroides and E. gallinarum had a significant effect (p ˂ 0.05) on the pH of PNAPMB except the interaction between P. pentosaceus and E. gallinarum (p = 0.631). The total titratable acidity (TTA) of the beverage ranged from 0.50 to 0.72%. All cultures had a significant influence (p ˂ 0.05) on the TTA of the beverage with the exception of the interaction between L. mesenteroides and E. gallinarum (p = 0.102). However, Monte Carlo simulation showed that E. gallinarum caused an increase in the pH and a decrease in the TTA of the beverage. During fermentation, the pH of the beverage is desired to decrease while the TTA increases, hence E. gallinarum was removed. The interaction between L. mesenteroides and P. pentosaceus at 0.05% and 0.025%, respectively produced an acceptable PNAPMB with potential for commercialization. Furthermore, moringa supplemented non-alcoholic pearl millet beverage (MSNAPMB) was produced by adding 4% of moringa (Moringa oleifera) leaf powder extract during the production of PNAPMB. The physicochemical, nutritional, microbial (LAB) and sensory characteristics of the PNAPMB, MSNAPMB and TNAPMB were determined. LAB were significantly (p < 0.05) affected by the fermentation period and increased from 3.32 to 7.97 log cfu/ml and 3.58 to 8.38 log cfu/ml in PNAPMB and SNAPMB, respectively. The pH of PNAPMB decreased from pH 5.05 to pH 4.14 while the pH of MSNAPMB decreased from pH 5.05 to pH 3.65 during the 18 h fermentation. The growth of LAB during fermentation had a significant effect (p < 0.05) on the pH of the beverages. The TTA increased from 0.14 to 0.22% and increased from 0.17 to 0.38%, in PNAPMB and MSNAPMB, respectively. The TTA of the beverage was affected significantly (p < 0.05) by the 18 h of fermentation. The protein content was 1.62, 2.17 and 1.50% in PNAPMB, MSNAPMB and TNAPMB, respectively. PNAPMB sample was deemed acceptable in comparison to the MSNAPMB. The total colour difference (ΔE) was 5.91 and 10.60 in PNAPMB and MSNAPMB, respectively in comparison to the TNAPMB. Volatile compounds with beneficial effect such as anti-inflammatory and anti-pathogenic properties were identified in the beverages. Principal component analysis indicated that the variations in characteristics of PNAPMB and MSNAPMB could be explained using total fat, saturated fat, total sugar, ash, moisture, proteins, chroma (C), hue and b*. The results showed that isolated pure cultures could be used as starter cultures in the production of non-alcoholic cereal beverages at a commercial level with predictable quality and safety properties.

Huang, Xun. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 73-87). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

Le nombre de sujets du panel détermine en grande partie le coût des études descriptives et hédoniques de l'analyse sensorielle. Une fois les risques α et β fixés, ce nombre peut théoriquement être calculé, dés lors que l'on connait la variabilité de la mesure due à l'hétérogénéité de la population visée et que l'on fixe la taille de la différence que l'on désire mettre en évidence. En général, l'ordre de grandeur du premier de ces paramètres est inconnu alors que celui du second est délicat à préciser pour l'expérimentateur. Ce travail propose une documentation systématique des valeurs prises dans la réalité par ces deux paramètres grâce à l'exploitation de deux bases de données, SensoBase et PrefBase, contenant respectivement un millier de jeux de données descriptives et quelques centaines de jeux de données hédoniques. Pratiquement, des recommandations pour la taille de panel sont établies sous forme d'abaques prenant en compte trois niveaux pour chacun des deux risques et des deux paramètres.D'autre part, ce travail étudie le nombre de sujets dans chacun des deux types de panel par une approche de ré-échantillonnage qui consiste à réduire progressivement le nombre de sujets tant que les résultats de l'analyse statistique demeurent stables. En moyenne, la taille des panels descriptifs pourrait être réduite d'un quart du nombre de sujets, mais cette moyenne cache une forte hétérogénéité selon le type de descripteurs considéré. La taille optimale des panels hédoniques serait elle très variable et cette variabilité est induite beaucoup plus par la nature et l'importance des différences entre les produits que par l'hétérogénéité des préférences individuelles. De plus, une même approche de ré-échantillonnage appliquée aux répétitions en tests descriptifs suggère que les répétitions ne sont plus nécessaires en phase de mesure, c'est-à-dire une fois le panel entraîné / The costs associated with sensory evaluation increase with the number of panelists to be enrolled. Classical power computation can be used to derive the minimal number of subjects of a sensory panel in order to control both type I (α risk) and type II (β risk) errors. However, this power computation requires estimates of the size of the product effect to be sought and of the residual variability of the ANOVA model used. Generally, both product effect size and residual variability are difficult to estimate a priori by the sensory analyst. This work offers estimations of these two parameters thanks to the analysis of hundreds descriptive andhedonic studies collected respectively in two databases, SensoBase and PrefBase. The meta-analysis of the data allowed to quantify these two parameters and made possible the calculation of the number of panelists. Hence, tables of panel sizes were proposed for 3 levels of respectively product effect size, residual variability and type I and II errors. Of course, this was done independently for descriptive and hedonic tests.Another approach based on resampling in numerous datasets was applied for both descriptive and hedonic studies. The method used to derive adequate panel size consisted in removing k subjects from the N of the original panel and then measuring the loss of information in product comparisons. For descriptive panels, panel size could be reduced by a quarter but this reduction strongly depends on the type of attributes. For hedonic panels, panel sizes varied extremely and depended mainly on the size of the liking differences between products to be compared. We expect that this difference is directly affected by the level of sensory complexity of the products. Finally, the resampling approach was applied to examine the need to replicate with trained sensory panels. Results suggested that replicates are no longer necessary at the testing phase, that is once the panel is trained

Our perception of the world is limited by the senses we are endowed with. In the case of taste, its functional fidelity is so critical for our survival that we come into the world with innate preference for sweet and disgust for bitter. These stereotyped behaviors are hardwired at the lowest levels of taste processing and they support the view that taste serves as an arbiter of the chemical world, passing judgement before permitting ingestion. Yet our experience of foods is manifold. This complexity results from distinct contributions from the sights, sounds and smells of the foods we consume. Of these, odors are a co-equal component of flavor and the impairment of olfaction can disrupt enjoyment of eating and alter patterns of consumption. The goal of this thesis is to identify the neural basis of odor-taste perception and to characterize how neural activity is affected by odor-taste integration. In contrast to the discrete and innate categorization performed by the taste system, the sense of smell enables discrimination of thousands of unique odor percepts which have no innate value. At the level of olfactory cortex, odor representations are randomly distributed and have been shown to be conditioned through association with other stimuli. The act of eating produces near simultaneous taste and odor transduction originating from the same source. Yet despite ultimately projecting to neighboring cortical regions, taste and odor pathways are anatomically segregated prior to reaching the cortex. Using viral tracing strategies, we identified Agranular Insular cortex (AIc) as a putative site of odor-taste integration. We then used in vivo two-photon Ca2+ Imaging to characterize odor and taste responsive neurons and identify changes in population activity when these stimuli were simultaneously presented. We next asked whether specific flavor experiences altered activity in AIc compared to naive animals. Finally, we developed a behavioral task to test whether silencing AIc disrupted perception of a flavor compound.

Little is known about how liking for different foods develops from birth to adulthood. Although there are both cultural and sensory aspects to the development of food preferences, the focus of this study is on the sensory aspects of food preference development, in particular, preferences for meat. Two main aims are addressed : 1/. To develop a robust methodology that can be used to determine pre-literate and recently literate children's liking for different foods and the determinants of that liking. 2/. To investigate the effects of early experience with foods on later food preferences.Several tests were conducted and results noted. Overall the results of this thesis show that it is possible to gather reliable hedonic data from young, even pre-school children, and that it is possible that very early feeding experience has some influence on adults' food preferences. / Doctor of Philosophy (PhD)

Radish anthocyanin extract has potential as a natural colorant because of its pigment stability and attractive red hue. Presence of undesirable aroma compounds could limit its applications in foods. The pungent principle of radish, 4-methylthio-3-butenyl isothiocyanate (MTBI), is produced enzymatically upon cell injury from its glucosinolate precursor, 4-methylthio-3-butenyl glucosinolate (MTBG), and undergoes subsequent degradation to produce a number of volatiles. To evaluate the potential of flavor formation, juices were prepared from winter and spring radish cultivars. Whole radishes, peels and flesh, as well as radish extracts, were analyzed for glucosinolates and isothiocyanates. Aroma intensities of radish juice extracts were evaluated using sensory analysis. MTBI was monitored by HPLC (detection level 160 ppb). MTBG was extracted from freeze-dried radish tissue with boiling methanol, purified by anion exchange and enzymatically desulfated. DesulfoMTBG was quantified by HPLC, using desulfosinigrin as internal standard. Identification was performed by fast atom bombardment and electrospray mass spectroscopy. MTBI formation was higher in winter than in spring cultivars (1.5-2.8 and 0.8-1.3 mg/100g fresh weight, respectively), and higher in flesh than in peels. MTBG ranged from 30-65 mg (spring cultivars) to 260-320 mg/100g fresh weight (winter cultivars) with greater concentration in peels than in flesh. Isothiocyanates and glucosinolates were not detected in radish juices. Overall aroma intensities of radish juice concentrates, diluted to 150, 300, 600 and 1200 mg anthocyanin/L in water, were rated using a 16-point scale. Radish concentrates from cultivars Fuego (pigmented peels) and Red Meat Takii (whole red flesh) were compared to commercial red cabbage and radish colorants. Aroma intensities followed first order relationships with anthocyanin concentrations. The commercial colorants were rated slight to moderate, while radish extracts (Fuego and Takii) were rated moderate to large. The aroma intensity of red flesh radish extract was more potent than those prepared from radish peels. Further work includes development of purification techniques which would provide an odorless aqueous extract. / Graduation date: 1997

Consumer preferences for legume cooking properties should be considered at an earlier stage in the breeding process. Hence, we developed an effective, low-cost method to analyze the cooking quality attributes of cowpeas. The objective was to develop a rapid screening method to evaluate the cooking quality attributes of cowpeas (Vigna unguiculata) and compare the results with currently used methods. Soaked samples (five grams) were boiled for 27 min, and seeds and their broth were separated into dishes with covers. Samples were subjectively rated on a 1-5 scale for cooked doneness, tactile texture, aroma intensity, and opacity of the broth. Water absorption, seed splitting, and soluble solid loss were also determined. The samples were evaluated in batches of 25 and replicated three times. Cooking properties showed significant correlations with each other, but did not correlate with raw seed size and color. The method is important because consumer acceptability largely depends on cooking quality in addition to seed appearance. Many properties like cooked doneness and tactile texture were significantly affected by genetics and environment. Compression force determined with a Texture Analyzer (TA) significantly correlated with doneness and tactile texture ratings at -0.67 and -0.69, respectively (P < 0.01). Cooking times from the Mattson bean cooker (MBC) were significantly correlated with doneness and tactile texture at -0.63 and -0.65, respectively (P < 0.05). The Texture Analyzer and MBC confirmed the subjective ratings of cooked doneness and tactile texture. A procedure to determine solid losses using a refractometer, instead of the time-consuming oven-drying method, saved time and has significant promise for use in simple evaluations. The rapid cooking method required 2 hr on the first day and 5 hr on the second day to evaluate 25 samples. The method is efficient, repeatable and uses inexpensive materials compared to the TA and MBC. It also provides descriptive information, and differentiates legume cultivars based on cooking properties. This method is a useful tool in the breeding program for selecting and advancing promising lines. Food processors may also use this method for a quick evaluation to check if their legumes meet required specifications for processing.