Bibliography: leaves 184-209. This thesis describes the occurence of natural, biologically based suppression of Rhizoctonia barepatch in a direct drilled system at Avon, South Australia. The supressive characteristics are transferable, removed by biocidal treatments, and active against increasing doses of R. solani AG-8, Gaeumannomyces graminis var. tritici and Fusarium graminearum. Disease severity and the viable population of Rhizoctonia are reduced in suppressive soil but the causal agent is still present. The microbial populations in suppressive and non-suppressive soil appear to differ both in their functioning and composition. The control strategy is developed through manipulation of the existing soil biota with farming practices.

Thesis (M.S.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains viii, 77 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 71-77).

No description available.

No description available.

Stachybotrys elegans (Pidopl.) W. Gams is a destructive mycoparasite of the soilborne plant pathogen Rhizoctonia solani. It colonizes effectively all types of cells of R. solani, and is considered as an effective biological control agent (BCA). Monitoring the presence of this mycoparasite in the field trials requires the development of a reliable and sensitive diagnostic assay that is able to detect and differentiate the BCA from their target host. To achieve this, designed SCAR (sequenced characterized amplified regions) primers designated as SE-13F and SE-13R were generated from informative RAPD markers. They were tested in conventional PCR assays alone or in conjunction with the recently developed SCAR primers (SBU-177/336) designed for Rhizoctonia solani (Kuhn) on several types of DNA. These included DNA extracted from pure cultures, co-cultures of the BCA and the pathogen, plant tissue and several types of soils inoculated with both the BCA and the pathogen. Irrespective of the type of the biological samples from which the DNA was extracted, the primers SE-13F/SE-13R successfully amplified only S. elegans. No cross-reaction was observed when the primers were used to amplify DNA of other fungi, bacteria and plant tissues. Likewise, the primer pair SBU-177/336 detected only its target organism, i.e., R. solani. The detection limit using these primers on amplified DNA was as little as 1 pg DNA extracted from pure cultures of S. elegans, 100 pg DNA extracted from greenhouse soil and 33 pg DNA extracted from natural soil. This work is the first report on the development of SCAR markers for the BCA, S. elegans. These molecular markers offer not only an alternative diagnostic assay to conventional detection methods, but also the possibility of being used in ecological studies.

Bibliography: leaves 149-173. / xiv, 173 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1994

Bibliography: leaves 184-209. / xx, 219 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis describes the occurence of natural, biologically based suppression of Rhizoctonia barepatch in a direct drilled system at Avon, South Australia. The supressive characteristics are transferable, removed by biocidal treatments, and active against increasing doses of R. solani AG-8, Gaeumannomyces graminis var. tritici and Fusarium graminearum. Disease severity and the viable population of Rhizoctonia are reduced in suppressive soil but the causal agent is still present. The microbial populations in suppressive and non-suppressive soil appear to differ both in their functioning and composition. The control strategy is developed through manipulation of the existing soil biota with farming practices. / Thesis (Ph.D.)--University of Adelaide, Dept. of Soil Science, 1996

Zugl.: Hohenheim, Univ., Diss., 2007

Univ., Diss., 2008--Kassel.

Techn. Universiẗat, Diss., 2006--München.