Thesis (Ph. D.)--University of Wisconsin--Madison, 1967. / Typescript. Vita. Description based on print version record. Includes bibliographical references (leaves 305-309).

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As the drone market expands, the corresponding standardization follows. Drone standardization can vary geographically based on the regulations and requirements of different areas. This study mainly focuses on the European Union Aviation Safety Agency (EASA) regulations and investigates Aerit’s role, as a drone operator in Sweden, within this standardization framework. In particular, Specific Operations and Risk Assessment (SORA) process, developed by EASA, is illustrated. The process covers a comprehensive range of factors related to drone operations to assess and manage risks. In addition to the drone design standardization process, the study looks into drone flight control systems at component-level redundancy and at system-level redundancy with a scientific grounding of dependability. An investigation of what a voting system looks like is then conducted for implementing a redundant flight control architecture. Furthermore, results from Software-In-The-Loop (SITL) implementation in this study show that the performance differs not much for the two flight control architectures (component-level and system-level). Thus, the decision of whether to use one flight controller or redundant flight controllers depends on the specific requirements and priorities of the drone application as well as the level of pre-flight testing. / Eftersom drönarmarknaden växer, följer motsvarande standardisering med. Standardisering av drönar kan variera geografiskt baserat på olika områdens lagar och krav. Denna studie fokuserar främst på Europeiska unionens byrå för luftfartssäkerhet (EASA) och undersöker Aerits roll som drönaroperatör i Sverige inom detta standardiseringsramverk. Särskilt beskrivs processen för Specifika Operationer och Riskbedömning (SORA), utvecklad av EASA. Denna process täcker ett omfattande utbud av faktorer relaterade till drönaroperationer för att bedöma och hantera risker. Utöver standardiseringsprocessen för drönardesign, granskar studien drönarflygkontrollsystem på komponentnivå för redundans och på systemnivå med en vetenskaplig grund för tillförlitlighet. En undersökning av hur ett röstningssystem ser ut genomförs sedan för att implementera en redundant flygkontrollarkitektur. Vidare visar resultaten från mjukvara-i-slingan (SITL) -implementeringen i denna studie att prestandan inte skiljer sig mycket mellan de två flygkontrollarkitekturerna (komponentnivå och systemnivå). Därför beror beslutet om att använda en flygkontroll eller redundanta flygkontroller på de specifika kraven och prioriteterna för drönapplikationen samt nivån av före-flygtestning.

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Call-response surveys were conducted to better delineate and estimate the population of the nationally threatened least bittern and their habitat requirements in southern Manitoba, Canada. Other marsh bird species whose populations are believed to be declining due to wetland loss throughout, or in parts of, their range were also surveyed including the American bittern, pied-billed grebe, sora, Virginia rail and yellow rail. Surveys were conducted during the 2003 and 2004 breeding seasons within 46 different wetlands. Least bitterns were encountered on 26 occasions at 15 sites within 5 wetlands. The sora was the most abundant and widely distributed target species and was encountered on 330 occasions in 39 of the 46 surveyed wetlands. Yellow rails were not detected during either survey year due to survey methodology. Use of the call-response survey protocol led to an increase in the numbers of all target species detected. This increase was more significant for the least bittern, sora and Virginia rail. Habitat was assessed as percent vegetation cover within a 50-m radius around the calling sites, and forest resource inventory data were used in a Geographic Information System to determine the landscape composition within a 500-m radius around the sites and within a 5-km radius around the wetlands surveyed. Logistic regression analyses were used to evaluate the relationship between the presence of the target species and the site and landscape characteristics. The target species responded differently to different site and landscape characteristics. Least bittern and pied-billed grebe selected areas with higher proportions of Typha spp. and tall shrubs; American bittern also selected areas with higher proportions of tall shrubs. At the 5-km scale, the American bittern responded positively to the amount of wetland and some positive trends were also detected for the pied-billed grebe. Sora and Virginia rail were not associated with any of the measured landscape characteristics. One of the most important steps towards the conservation of marsh bird species in Manitoba and elsewhere is the development, adoption, and implementation of a standardized survey protocol. Based on the results of the present study, I recommend that future surveys include both a passive and call-broadcast period for marsh bird species. Future surveys should be conducted in both the morning and evening and sites should be visited 3 times each during the breeding season. In southern Manitoba, call-response surveys should begin as early as the beginning of May to ensure the survey incorporates the period of peak vocalization. I recommend that future yellow rail surveys be conducted after dark. In this study many of the target species selected sites that had a greater area of wetland habitat surrounding them. Future wetland conservation efforts should focus on the protection and/or restoration of wetland complexes to ensure that remaining wetlands do not become smaller and increasingly isolated from one another. In addition, the Rat River Swamp was found to be the most productive marsh complex for least bittern in southern Manitoba. Measures should be taken to protect this area from future development and alteration. / May 2006

Call-response surveys were conducted to better delineate and estimate the population of the nationally threatened least bittern and their habitat requirements in southern Manitoba, Canada. Other marsh bird species whose populations are believed to be declining due to wetland loss throughout, or in parts of, their range were also surveyed including the American bittern, pied-billed grebe, sora, Virginia rail and yellow rail. Surveys were conducted during the 2003 and 2004 breeding seasons within 46 different wetlands. Least bitterns were encountered on 26 occasions at 15 sites within 5 wetlands. The sora was the most abundant and widely distributed target species and was encountered on 330 occasions in 39 of the 46 surveyed wetlands. Yellow rails were not detected during either survey year due to survey methodology. Use of the call-response survey protocol led to an increase in the numbers of all target species detected. This increase was more significant for the least bittern, sora and Virginia rail. Habitat was assessed as percent vegetation cover within a 50-m radius around the calling sites, and forest resource inventory data were used in a Geographic Information System to determine the landscape composition within a 500-m radius around the sites and within a 5-km radius around the wetlands surveyed. Logistic regression analyses were used to evaluate the relationship between the presence of the target species and the site and landscape characteristics. The target species responded differently to different site and landscape characteristics. Least bittern and pied-billed grebe selected areas with higher proportions of Typha spp. and tall shrubs; American bittern also selected areas with higher proportions of tall shrubs. At the 5-km scale, the American bittern responded positively to the amount of wetland and some positive trends were also detected for the pied-billed grebe. Sora and Virginia rail were not associated with any of the measured landscape characteristics. One of the most important steps towards the conservation of marsh bird species in Manitoba and elsewhere is the development, adoption, and implementation of a standardized survey protocol. Based on the results of the present study, I recommend that future surveys include both a passive and call-broadcast period for marsh bird species. Future surveys should be conducted in both the morning and evening and sites should be visited 3 times each during the breeding season. In southern Manitoba, call-response surveys should begin as early as the beginning of May to ensure the survey incorporates the period of peak vocalization. I recommend that future yellow rail surveys be conducted after dark. In this study many of the target species selected sites that had a greater area of wetland habitat surrounding them. Future wetland conservation efforts should focus on the protection and/or restoration of wetland complexes to ensure that remaining wetlands do not become smaller and increasingly isolated from one another. In addition, the Rat River Swamp was found to be the most productive marsh complex for least bittern in southern Manitoba. Measures should be taken to protect this area from future development and alteration.

Call-response surveys were conducted to better delineate and estimate the population of the nationally threatened least bittern and their habitat requirements in southern Manitoba, Canada. Other marsh bird species whose populations are believed to be declining due to wetland loss throughout, or in parts of, their range were also surveyed including the American bittern, pied-billed grebe, sora, Virginia rail and yellow rail. Surveys were conducted during the 2003 and 2004 breeding seasons within 46 different wetlands. Least bitterns were encountered on 26 occasions at 15 sites within 5 wetlands. The sora was the most abundant and widely distributed target species and was encountered on 330 occasions in 39 of the 46 surveyed wetlands. Yellow rails were not detected during either survey year due to survey methodology. Use of the call-response survey protocol led to an increase in the numbers of all target species detected. This increase was more significant for the least bittern, sora and Virginia rail. Habitat was assessed as percent vegetation cover within a 50-m radius around the calling sites, and forest resource inventory data were used in a Geographic Information System to determine the landscape composition within a 500-m radius around the sites and within a 5-km radius around the wetlands surveyed. Logistic regression analyses were used to evaluate the relationship between the presence of the target species and the site and landscape characteristics. The target species responded differently to different site and landscape characteristics. Least bittern and pied-billed grebe selected areas with higher proportions of Typha spp. and tall shrubs; American bittern also selected areas with higher proportions of tall shrubs. At the 5-km scale, the American bittern responded positively to the amount of wetland and some positive trends were also detected for the pied-billed grebe. Sora and Virginia rail were not associated with any of the measured landscape characteristics. One of the most important steps towards the conservation of marsh bird species in Manitoba and elsewhere is the development, adoption, and implementation of a standardized survey protocol. Based on the results of the present study, I recommend that future surveys include both a passive and call-broadcast period for marsh bird species. Future surveys should be conducted in both the morning and evening and sites should be visited 3 times each during the breeding season. In southern Manitoba, call-response surveys should begin as early as the beginning of May to ensure the survey incorporates the period of peak vocalization. I recommend that future yellow rail surveys be conducted after dark. In this study many of the target species selected sites that had a greater area of wetland habitat surrounding them. Future wetland conservation efforts should focus on the protection and/or restoration of wetland complexes to ensure that remaining wetlands do not become smaller and increasingly isolated from one another. In addition, the Rat River Swamp was found to be the most productive marsh complex for least bittern in southern Manitoba. Measures should be taken to protect this area from future development and alteration.

Epithelial-mesenchymal transition (EMT) is a trans-differentiation program essential for development and wound healing that is pathologically activated during cancer progression. During this process, cells undergo complex changes at the transcriptional and translational levels leading to dissolution of cell-cell junctions, loss of apical-basal polarity, and cytoskeleton reorganization. Transforming Growth Factor-β (TGF-β) is well-established in driving cancer progression through EMT induction. Remodeling of cellular junctions, including gap junctions, is critical to acquiring migratory and invasive characteristics during EMT. The gene GJA1 encodes for Connexin43 (Cx43), the most ubiquitously expressed gap junction protein where altered regulation of Cx43 is associated with cancer progression. Intriguingly, Cx43 mRNA undergoes alternative ‘internal’ translation initiation, generating N-terminally truncated isoforms, including GJA1-20k, which regulates Cx43 gap junction formation. We have previously demonstrated GJA1-20k expression is inhibited during TGF-β-induced EMT, limiting gap junction formation; however, the relationship between GJA1-20k modulation of gap junction localization and cellular invasion and migration remains unknown. Given the role GJA1-20k has in regulating gap junctions, we hypothesize that suppression of GJA1-20k expression promotes metastatic trait acquisition through limiting gap junction formation. Utilizing lentivirally transduced stable mouse mammary gland epithelial (NMuMG) and triple-negative human breast epithelial (MDA-MB-231) cells expressing GJA1-20k, or Lac Z as control, we tested effects on TGF-β-induced EMT induction and metastatic trait induction. Boyden chambers, would/scratch assays were employed to analyze cell invasion and migration respectively. We found GJA1-20k overexpression during EMT results in decreased cell invasion and migration to LacZ controls. Future directions include evaluation of GJA1-20k restoration in a metastatic breast cancer model in vivo. Investigating the underlying role of GJA1-20k in EMT-induced cell junction remodeling could be promising as a potential pharmacological target process independent of transcriptional or post-translational pathways. Ultimately, by adding novel information in the expanding and compelling field of translational control, this work could aid in developing the future of precision medicine as new therapeutic solutions to treat cancer will require limiting cancer cell’s ability to metastasize. / R01 HL132236 JWS R41 CA250874 SL R01HL132236 Diversity Supplement KLY II 23PRE1025483 AHA Predoctoral Fellowship KLY II / Doctor of Philosophy / Every organ system relies upon cell-to-cell communication to properly function and is the basis of multi-cellular life. Gap junctions are nanoscale conduits allowing the passage of small signaling molecules and ions between adjacent cells, similar to telephone tubes. Gap junctions are formed from proteins called connexins. Interestingly, it is well known that shorter pieces of connexin proteins can regulate the formation of gap junctions and are uniquely created by a process called alternative ‘internal’ translation. Changes in the amounts of short-length and full-length connexin proteins are often found in cancer cells. Cancer is the uncontrolled growth of abnormal cells. Commonly, the morphology of cancer cells, and the way they communicate with neighboring cells, is altered. Cancer progression is aided by changes in cell signaling molecules, including TGF-β which can drive cancer cells to leave primary tumor sites and grow elsewhere in the body. This is important for the cancer cells to continue dividing and eventually metastasizing (invading other organ systems). Treating cancer once it has spread to other regions of the body is difficult and is the main cause of cancer deaths worldwide. Using TGF-β to model metastatic changes in mouse and human cell lines, we studied how short-length connexin protein affects metastatic cancer cell behavior. With this information we will be able to guide the development of druggable alternative ‘internal’ translation targets, by restoring the proper communication between neighboring cells and therefore preventing spread of cancer cells.

No description available.