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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
11

Patterns of Genetic Variation in <i>Festuca hallii</i> (Vasey) Piper across the Canadian Prairie

Qiu, Jie 30 July 2009 (has links)
<i>Festuca hallii</i> [(Vasey) Piper] (plains rough fescue) is a dominant native grass species in the Fescue Prairie region of North America that has undergone dramatic range reduction in the past century. This research is undertaken to address the related issues associated with the effectiveness of sampling in capturing genetic diversity, the influence of habitat fragmentation on genetic variation, the geographic variation of seed germination characteristics, and the comparative genetic variation of differential germination. It was found that the tiller samples revealed slightly larger among-population variation than the seed samples. The fescue plant was genetically diverse, as revealed by the proportion of polymorphic bands, the mean band frequency, and the within-population variation. The genetic variation was not highly differentiated with only 6.5% of the total AFLP variation residing among populations. Mantel test revealed a significant correlation between genetic and geographic distances and a spatial autocorrelation up to 60 km among populations was detected. Base temperatures (<i>T<sub>b</sub></i>, minimal or base temperature permitting germination) of the 15 populations fell into a narrow range within 2.2°C with an average of 1.1°C. High final germination percentage was reached at a wide temperature range from 5 to 20°C with the highest germination percentage at 10°C. Germination rate index increased with increasing temperature from 5 to 20°C. <i>T<sub>b</sub></i> was positively correlated with latitude and negatively with longitude and the thermal time requirement for 50% germination was negatively correlated with latitude. The AFLP variation and germination responses were significantly associated with environmental attributes related to moisture, indicating local adaptation. However, the AFLP variation and germination was not significantly associated with the estimated population size and geographic distance to the nearest neighbor, suggesting that fragmentation has not generated considerable genetic and germination impact on the fescue populations. Marked differences in estimates of mean band frequency were observed for various groups of germinating seeds under different test temperatures. Comparisons of AFLP variation among 27 groups of seeds representing population, germination timing and test temperature indicates seed genotypes respond slightly differently to environmental variation, resulting in significant but small impact of germination timing and temperature on the genetic diversity of populations. These findings are significant not only for understanding and predicting the ecological adaptation of the species, but also for formulating effective restoration strategies for remnant populations.
12

Spatial variations in soil and plant delta 13 C and delta 15 N values in a subtropical savanna: implications for vegetation change and nutrient dynamics

Bai, E 15 May 2009 (has links)
Grass-dominated ecosystems in many regions around the world have experienced increased abundance of woody plants during the past 100 yrs. In the Rio Grande Plains of southern Texas, subtropical woodlands, dominated by C3 trees/shrubs capable of symbiotic N2-fixation, have become significant components of landscapes that were once dominated by C4 grasslands. Upland areas in this region now consist of small discrete clusters and large groves of woody vegetation embedded in a grassy matrix, while lower-lying portions of the landscape are dominated by closed-canopy woodlands. I used soil δ13C in conjunction with aerial photography and geostatistics to quantify landscape-scale vegetation dynamics in uplands of this savanna parkland. Spatial patterns of soil δ13C in grids and transects traversing woody patches indicated larger woody groves were formed from small discrete clusters of woody plants that spread laterally and eventually coalesced. Soil δ13C contour maps revealed some clusters are currently growing rapidly towards each other and might coalesce into groves in the near future, while some clusters remained relatively stable. Kriged maps of soil δ13C provided a strong spatial context for future studies aimed at understanding the functional consequences of this change in landscape structure. The dominant invading woody plant, honey mesquite (Prosopis glandulosa), was important in determining the spatial pattern of soil δ13C, supporting the hypothesis that they serve as recruitment foci and facilitate the establishment of subordinate woody species. Leaf δ15N values suggested that the N2-fixing mesquite influenced the N nutrition of nearby non-N2-fixing shrubs, thus, suggesting a mechanism by which mesquite could facilitate establishment of other woody species. In closed-canopy drainage woodlands, however, spatial patterns of soil δ13C were no longer controlled by the presence of mesquite, but by the amount of soil organic carbon and soil texture. The positive correlation between silt+clay and soil δ13C indicates that the formation of organomineral complexes and microaggregates may slow SOC turnover rates and favor the persistence of C4-derived SOC from the original grassland. This study enhances our understanding of potential patterns, causes and consequences of grassland to woodland conversions which are underway today in many grass-dominated ecosystems around the world.
13

Temporal and Spatial Variations of Arginine Kinase Allozyme Among Populations of Thais clavigera From the West Coasts of Taiwan

Wu, Jing-ying 23 February 2004 (has links)
The variation of Arginine kinase (Ark) genotype frequency among populations of Thais clavigera has been reported in previous study. However, the difference which is originated from self-seeding or differential selection after recruitment is not understood. To answer this question, the spatial (i.e. Shainsan, Taisi, Budai, and Chiku) and temporal (sell length: <15mm, 15-20mm, 20-30mm, 30-40mm, and >40mm; year: 2001 and 2003) variations of Ark allozyme were examined by starch gel electrophoresis. There was no significant difference in the ratio of Ark genotypes (ark63/63, ark63/100, ark100/100) in the size range of <15mm among Shainsan, Taisi and Chiku. The result indicated that the populations of T. clavigera is from the same recruitment. In the size range of 15-20mm, the frequency of ark63/63 increased from southern to northern populations, i.e. 0.21(Chiku) < 0.33(Budai) < 0.60(Taisi) < 0.66(Shainsan), respectively. It is proposed that the variation of Ark genotypes among populations is resulting from stress of temperature or dessication. A significant difference in the ratio of Ark genotypes was found in the population of Taisi with the size range of 30-40mm. Besides, the frequency of Ark genotypes among populations was not related to the degree of imposex.
14

The ecological impacts of invasive Pinus radiata in eucalypt vegetation: pattern and process

Williams, Moira Caroline January 2008 (has links)
Doctor of Philosophy (PhD) / Early recognition of plant invaders is key to their successful management. Yet knowledge of the ecological impacts of species before they become widespread is poor. This thesis examines the ecology of invasive Pinus radiata, a species which is known to spread from introduced plantings in Australia but is currently a low profile invader. Pinus invasions are considered major ecological problems in New Zealand and South Africa where wildlings are beginning to dominate natural areas and suppress native vegetation. Invasion success elsewhere and the large softwood estate in Australia suggest that pines may begin to dominate native eucalypt forests bordering large commercial plantations. This research focused on three components of impact of P. radiata; extent, abundance and effect per individual. The borders of 29 P. radiata plantations in NSW were surveyed in order to quantify the current level of invasion and to identify factors facilitating pine spread. Of particular interest was the role of propagule pressure, vegetation type and fire in the invasion process. The area of land in NSW currently invaded by P. radiata was estimated at almost 4 500 ha, although this is likely to be an underestimate due to an inability to detect wildlings (self-sown pines) at long distances from the plantation. Twenty six of the 29 plantations produced wild pines, however most of the sites are in the very early stages of invasion. Noticeable wildling populations were recorded at nine sites indicating that P. radiata is capable of establishing within native vegetation. Pine spread was most severe in the world heritage listed Blue Mountains region where pine densities reached up to 2000 per hectare in areas adjacent to the plantation and isolated pines were recorded up to 4 km from the source. The presence of isolated pines within intact native vegetation suggests that disturbance is not required for pine establishment in forested environments. Furthermore, high pine emergence and survival rates in eucalypt woodland and evidence of self reproduction by wildlings suggest that in the absence of adequate control measures pines may become established invaders in the Australian landscape. While low levels of current invasion at many sites hindered the ability to examine the factors facilitating invasion some variables that appear to be driving pine success were identified. At the landscape scale plantation size and residence time were significant predictors of the level of invasion at a site. Areas of native vegetation vi adjacent to plantations less than 40 years experienced very low levels of invasion suggesting a lag period between plantation establishment and invasion. However, pines with diameters up to 60 cm were observed growing adjacent to plantations younger than 40 years implying that the first colonisers are capable of establishing soon after plantation trees become reproductive. Propagule pressure was also found to have a strong influence on invasion success on a smaller scale manifesting in a significant positive relationship between the age of a plantation compartment and the likelihood of invasion. A negative relationship between plantation size and level of invasion was a surprising result and was influenced by just two large sites that happened to be located in areas of high rainfall. All sites receiving more than 1300 mm annual rainfall experienced low levels of invasion suggesting that this is a limiting factor for pine spread in NSW. There were significant differences in the level of invasion between vegetation types implying that some communities are more susceptible to invasion. Patterns of spread confirmed ideas regarding the facilitative effect of disturbance in the invasion process and the resistance of wet sclerophyll forest to invasion in Australia. An absence of wildlings in cleared land and areas of remnant bushland was attributed to high levels of grazing pressure. Wind direction did not appear to influence the distribution of pines close to the plantation, but evidence of long distance wind dispersal of pines was provided by an investigation of pine spread from the air at one site where large pines were found growing 10 km downwind from a mature plantation. Fire was found to have both a positive and negative influence on the invasion process. High intensity wildfires are capable of destroying large pines with diameters exceeding 50 cm. However, fire can stimulate seed release from cones resulting in large post-fire recruitment pulses. Seedling densities of up to 3050 per hectare were recorded almost 3 years after wildfire, suggesting that follow up control prior to recruits reaching coning age, i.e. within 5 years, would be beneficial. Surveys of wildling pines exposed to low intensity hazard reduction burns suggest that the majority of pines greater than 3 m in height and with a diameter of more than 10 cm will survive the fire. Low intensity prescribed fires that are carried out after pines have reached this size will fail to control wildling populations. To examine the influence of P. radiata once it has established in the native community this study focused on two mechanisms of impact, the addition of pine litter and increased shade due to an increase in canopy cover. Collection of pine litterfall vii within an invaded eucalypt woodland over a 2 year period recorded rates of up to 1400 kg/ha/year in the most heavily invaded area with a pine basal area of 11.3m2/ha. More than 70 % of pine litter fell directly below the pine canopy suggesting that the most severe litter effects will be limited to these areas. Glasshouse and field experiments were conducted to examine the influence of this increased litter load on the emergence of P. radiata and two native species. Recruitment of native plant species was impeded by litter levels of 6000 kg/ha, the equivalent of approximately 4 years of pine litterfall. Both P. radiata and the two natives responded similarly to pine and eucalypt litter suggesting the two litter types are influencing the recruitment phase equally. However, where pines are added to the system, increased litterfall rates could potentially result in the doubling of the litter load and hence a greater barrier to seedling establishment. Pine invaded eucalypt woodlands are also subjected to three fold increases in canopy cover. Trends in reduced emergence of native species under a pine canopy suggest that the addition of pines to eucalypt forests is likely to have a negative influence on native recruitment and may result in a shift towards a shade tolerant community. However, reversal of trends in emergence below pine canopy between seasons implies that quantifying invasion impacts requires a consideration of temporal variation. Increased levels of disturbance, forest fragmentation and an increasing pine estate are likely to lead to the infestation of new areas. Furthermore the lag phase associated with pine spread means that even if no new plantations are established the number of invasion events will increase. This study has identified a number of risk factors that can be used to guide plantation establishment and the management of invasion events. Minimising disturbance at plantation borders and increasing the ‘no planting’ zone will help to reduce the impacts of pines. Where possible new plantations should be established upwind of cleared land or at least, wet sclerophyll forest. Frequent monitoring of the borders of plantations yet to source invasions, particularly those greater than 40 years of age, will help identify problem areas before control becomes difficult and costly. Maps of the 29 plantations marked with areas of pine infestation will help prioritise sites for control and provide base level knowledge for future monitoring of pine spread. Stringent legislation that binds plantation managers to control wildlings beyond their boundaries is critical for the effective management of pine invasions. With infinite numbers of invaders and limited funds to dedicate to their control, a method of triaging species for management is critical. This is particularly difficult viii when information is typically biased towards invaders that are already widespread. By focussing on the ecological impacts of invaders it becomes possible to rank species on the basis of the threat they pose to native communities. Ecological research is capable of providing the knowledge to quantify invasion impacts and must remain at the centre of policy decisions.
15

Spatiotemporal Analysis of Escherichia coli along metro-Atlanta Surface Waters

Orquiola, Diana 08 August 2017 (has links)
Introduction: The Chattahoochee River and its lakes and tributaries are essential resources for metro-Atlanta; however, urbanization and other human impacts near these resources affects the quality of water. The objectives of this study are to describe the spatial and temporal patterns of Escherichia coli (E. coli), and examine the relationship between rainfall and E. coli levels in metro-Atlanta surface waters, including the Chattahoochee River and its lakes and tributaries. Methods: The water quality data used for the study was originally collected and analyzed by the Chattahoochee Riverkeeper (CRK) Neighborhood Water Watch (NWW) program. For this analysis, sites with a total of 50 samples or more were subjected to descriptive analysis. Results: Twenty-seven of the 36 sites had a mean E. coli concentration exceeding the US EPA’s recreational water quality criteria of 2.37 log Most Probable Number per 100 milliliters. Sites with the highest and lowest mean E. coli levels were located, on average, 2.77 and 20.22 miles from downtown Atlanta, respectively. Spikes in E. coli levels occurred 99 counts of 699 sampling events in the winter, 168/827 spring, 141/877 summer, and 170/950 fall. Seven of the 36 sites were analyzed to examine the relationship between 48-hour rainfall and E. coli levels. Four sites indicated that there was a significant positive correlation between the two variables. (r=0.52, p<.0001; r=0.67, p<.0001; r=0.39, p<.0001; r=0.38, p<.0001) Discussion: The water quality in waterways closer to downtown may be attributable to combined sewer overflows and the urbanization and land use associated with the city. Overall, there was temporal variation in E. coli concentrations, however there was no distinct seasonal pattern. The relationship between 48-hour rainfall and E. coli levels of four of the seven sites analyzed was consistent with other studies.
16

Disparate Growth in Hamilton's Central Area

Manojlovic, Drazen 04 1900 (has links)
<p> This paper attempts to quantify disparate growth in Hamilton's Central Area. The spatial variation over time of three economic indicators was studied to do this. These indicators were property tax assessments, and multi-family and single-family property sales. The Central Area was divided into four geographic zones so that the indicator change could be associated with different parts of the Area. The results indicate that the northeastern sections of the Central Area experienced and are continuing to experience slower economic growth compared to the southwestern sections. </p> / Thesis / Bachelor of Arts (BA)
17

Variação espacial e temporal de microcrustáceos planctônicos do lago Monte Alegre e experimentos abordando a influência da qualidade do alimento sobre o desempenho dos cladóceros / Spatial and temporal variation of planktonic microcrustaceans of lake Monte Alegre and experiments approaching the influence of food quality on the performance of cladocerans

Souza, Bruno Barretto de 08 May 2015 (has links)
Este trabalho está dividido em dois capítulos. O primeiro aborda a distribuição espacial de microcrustáceos planctônicos nas zonas litorânea e limnética do lago Monte Alegre, situado em Ribeirão Preto, estado de São Paulo, e a influência de fatores abióticos e bióticos sobre o desenvolvimento e a distribuição. Este capítulo utiliza dados de Ferreira (2013) referentes à zona limnética e constitui um manuscrito, que deverá ser submetido para publicação; os dados coletados e analisados neste trabalho foram os referentes à zona litorânea, bem como todo tratamento estatístico para a comparação entre as duas zonas. Foram analisadas amostras semanais do zooplâncton e seus predadores invertebrados, obtidas no mesmo período nas zonas litorânea e limnética, durante um ano, com o objetivo de estudar a flutuação ao longo do ano, sua reprodução e quais espécies de microcrustáceos planctônicos ocupam o litoral do lago; os fatores físicos e químicos avaliados no mesmo período das coletas foram utilizados para determinar as condições em que os organismos se encontram nas duas zonas. A distribuição das espécies planctônicas mais abundantes ocorreu em ambas as zonas, sendo que Bosmina tubicen e Diaphanosoma birgei mostraram preferência pela zona litorânea, enquanto Daphnia gessneri foi mais abundante na zona limnética e Ceriodaphnia richardi e Daphnia ambigua foram indiferentes quanto a sua distribuição nas zonas litorânea e limnética. Os copépodos distribuíram-se preferencialmente na zona pelágica. Maior concentração de carbono algal foi registrada na zona limnética em relação à zona litorânea, porém algumas algas podem apresentar características indigestas. Nossos dados indicam que o litoral possui condições favoráveis para o desenvolvimento de cladóceros planctônicos, sendo que a preferência de algumas espécies por essa zona poderia ser uma adaptação para diminuir a predação por invertebrados limnéticos. Embora o litoral tenha outros predadores, a mortalidade na zona limnética deve ser maior. O capítulo dois apresenta a distribuição horizontal da comunidade de microcrustáceos, especialmente os cladóceros, em três zonas do lago Monte Alegre - no banco de macrófitas, na borda do banco de macrófitas e na zona limnética - e o efeito desta distribuição sobre sua reprodução e tamanho. As amostragens de zooplâncton e medições de fatores físicos e químicos foram realizadas durante 18 dias, em intervalos de 3 dias. Também foram realizados experimentos em laboratório para analisar a influência da quantidade e qualidade do alimento sobre os parâmetros de tabela de vida de duas espécies de cladóceros encontradas no lago, utilizando o séston de cada zona. Fatores físicos e químicos do séston também foram avaliados no período dos experimentos. D. birgei mostrou preferência pela zona litorânea e D. ambigua pela zona limnética, enquanto C. richardi e D. gessneri não apresentaram preferência significativa. A qualidade do alimento não influenciou de maneira negativa o desenvolvimento de C. richardi e D. birgei, no período dos experimentos, podendo não ser o fator responsável por sua distribuição no lago. Já D. birgei apresentou diferenças significativas entre as zonas, quanto ao total de neonatos por fêmea, fecundidade média e máximo de neonatos por prole. Dessa maneira, a zona litorânea do lago Monte Alegre mostrou- se propícia ao desenvolvimento de cladóceros planctônicos; a qualidade do séston nesta zona não influencia de maneira negativa os parâmetros de tabela de vida de C. richardi e D. birgei. / This work is divided into two chapters. The first deals with the spatial distribution of planktonic microcrustaceans in the littoral and limnetic zones of the Lake Monte Alegre, located in Ribeirão Preto, São Paulo, and the influence of abiotic and biotic factors on the development and distribution. This chapter uses data from Ferreira (2013), concerning the limnetic zone and is a manuscript to be submitted for publication; the data analyzed in this study were those related to the littoral zone, as well as all the statistical analysis for comparison between the two zones. Weekly samples of zooplankton and their invertebrate predators, obtained in the same period in both zones were analyzed for one year, with the aim of studying the fluctuation throughout the year, its reproduction and which species of planktonic microcrustaceans occupy the littoral; physical and chemical factors were evaluated in the same period of the plankton samples to determine the conditions for the organisms in the two zones. The most abundant planktonic microcrustaceans distributed in both zones, Bosmina tubicen and Diaphanosoma birgei showing preference for the littoral, while Daphnia gessneri was more abundant in the limnetic zone and Ceriodaphnia richardi and Daphnia ambigua were indifferent, regarding both zones. Copepods occupied preferentially the pelagic zone. Higher concentration of algal carbon was recorded in the limnetic zone in relation to the littoral zone, but some algae may have indigestible features. Our data indicate that the shoreline of the lake has favorable conditions for the development of planktonic cladocerans, and the preference of some species for this area could be a strategy to reduce predation by limnetic invertebrates. Although the littoral has other predators, mortality in the limnetic zone might be higher. Chapter two presents the horizontal distribution of planktonic microcrustaceans, especially cladocerans, in three areas of the Lake Monte Alegre - within macrophyte stands, the edge of macrophyte stands and in the limnetic zone - and the effect of this distribution on their reproduction and size. Zooplankton samples and measurements of physical and chemical factors were carried out for 18 days at intervals of 3 days. Laboratory experiments were also performed to analyze the influence of the food quantity and quality on the life table parameters of two species of cladocerans from the lake, using the seston of each zone. Physical and chemical factors of the seston were also assessed during the experiments. D. birgei showed preference for the littoral and D. ambigua for the limnetic zone, while C. richardi and D. gessneri were indifferent. The food quality did not influence negatively the development of C. richardi and D. birgei and could be not the main factor responsible for their distribution in the lake. D. birgei showed significant differences between zones, such as the total neonates produced per female, average fertility, and maximum offsprings per clutch. Thus, the littoral zone of the Lake Monte Alegre was favorable to the development of planktonic cladocerans at the time of the experiments, the seston quality not influencing negatively the life table parameters of C. richardi and D. birgei.
18

Estrutura da fauna de invertebrados bentônicos de dois riachos da Bacia do Rio Paranapanema (São Paulo, Brasil)

Alves, Maria Ines Bulgari January 2017 (has links)
Orientador: Virgínia Sanches Uieda / Resumo: A remoção da vegetação ripária pode ser uma importante causa de redução da diversidade e alterações na distribuição de invertebrados bentônicos ao longo do curso de riachos. O presente trabalho foi realizado em trechos de terceira ordem de dois riachos, um trecho com mata ripária (fechado) e outro sem (aberto) em cada riacho, e em dois meses da estação seca de dois anos consecutivos, a fim de verificar possíveis alterações espaciais e temporais na estrutura da fauna de invertebrados bentônicos. Também foram coletados dados de 12 variáveis ambientais e analisados junto com a abundância da fauna com o objetivo de verificar a interação entre estes dois grupos de dados na diferenciação entre as quatro amostras realizadas (dois trechos e dois anos, por riacho). A manutenção ou retirada da vegetação ripária não atuou isoladamente sobre a estrutura da fauna de invertebrados bentônicos, a qual foi também influenciada pela posição (montante ou jusante) do trecho fechado e por características estruturais próprias de cada trecho estudado, como presença de intervenções antrópicas nas proximidades (açudes) e tipo de substrato do leito. No riacho que apresenta o trecho fechado a montante, a mata contribui com matéria orgânica em suspensão e detritos vegetais para o trecho aberto a jusante, aumentando a oferta de abrigo e alimento para a fauna, diversificada e abundante nos dois trechos. Por outro lado, a grande quantidade de macrófitas no trecho aberto, localizado a montante no outro riach... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Removal of riparian vegetation can be an important cause of reduced diversity and changes in the distribution of benthic invertebrates along streams. The present work was carried out in third order stretches of two streams, one stretch with riparian (closed) and one without (open) forest in each stream, and in two months of the dry season of two consecutive years, in order to verify possible changes Spatial and temporal changes in the fauna structure of benthic invertebrates. Data were also collected from 12 environmental variables and analyzed together with the abundance of the fauna, in order to verify the interaction between these two data groups in the differentiation between the four samples (two stretches and two years, by stream). The maintenance or removal of riparian vegetation did not act alone on the structure of the benthic invertebrate fauna, which was also influenced by the position (upstream or downstream) of the closed section and by the structural characteristics of each section studied, such as the presence of anthropic Proximities (weirs) and type of substrate of the bed. In the creek that presents the enclosed stretch upstream, the forest contributes with organic matter in suspension and vegetal debris for the open stretch downstream, increasing the offer of shelter and food for the fauna, diversified and abundant in both stretches. On the other hand, the large amount of macrophytes in the open section, located upstream in the other stream, provides a hete... (Complete abstract click electronic access below) / Mestre
19

Temporal and spatial variations of cyanobacteria in Karori Reservoir, Wellington

Prentice, Matthew James January 2008 (has links)
The Lower Karori Reservoir (LKR) is a small, monomictic lake of 2.34 ha situated in the Karori Wildlife Sanctuary (KWS), Wellington. Over the past decade cyanobacterial blooms have become a common occurrence in this water body. In 2005 Anabaena planktonica was detected for the first time in the LKR and this species now forms dense blooms during summer. These blooms are problematic as they reduce aesthetic appeal and have resulted in odour problems for visitors to this high profile wildlife sanctuary. The objectives of this study were to identify key physical, chemical and biological variables influencing phytoplankton dynamics in the LKR and to use ecological models to investigate plausible management options. The key parameters investigated, that may cause bloom formation were; summer stratification, high nutrient levels, and the food web effects of a large population of European perch (Perca fluviatilis). High resolution sampling was carried out every six hours over a 72 hour period during pre-bloom, bloom and post-bloom periods in 2006/7 to elucidate short term temporal and spatial variations in biological and physico-chemical parameters. Quantitative polymerase chain reaction (QPCR) was used to enumerate A. planktonica populations, allowing a large number of samples to be simultaneously evaluated. Algal densities were also estimated using conventional phytoplankton enumeration and chlorophyll a analysis. Water samples were collected for nutrient analysis at discrete depths and profiles were taken for temperature, dissolved oxygen and photosynthetic active radiation. Secchi depth and pH were also measured. Weekly or fortnightly phytoplankton and zooplankton samples and physical variables have been collected at LKR since September 2005 as part of an independent sampling program carried out by the KWS, Waikato University and Cawthron Institute. In this project the 2-year data set was used to assist with analysis of lake processes and for validation of the hydrodynamic-ecological model DYRESM-CAEDYM. Between 12 and 15 February, 2007, electric fishing was undertaken within the LKR. A total of 3,946 P. fluviatilis were removed and the effects on phytoplankton and zooplankton concentrations were investigated. To increase knowledge of the physiology of A. planktonica, laboratory experiments were undertaken using cultures subjected to a range of different light intensities and temperature regimes The phytoplankton assemblage of the LKR shows very distinct temporal variations. Summer stratification occurred in the LKR for ~4 months each summer. During these periods A. planktonica comprised up to 99.9% of the surface phytoplankton population. During isothermy chlorophytes, bacillariophytes and small flagellated dinophytes are co-dominant in the phytoplankton assemblage. The results of the QPCR showed distinct diurnal vertical movement of A. planktonica, with the highest cell concentrations occurring at 1900 hours at the surface. Ammonium (NH4-N) is the dominant species of inorganic nitrogen during periods of stratification, while nitrate (NO3-N) is generally dominant during times of isothermy. Phosphate concentrations at surface and depth remained at low levels throughout the sampling period. The large surface populations of A. planktonica, are probably responsible for the elevated total nitrogen concentrations in surface waters during stratified periods. There appeared to be some short term effects of the P. fluviatilis removal with an increase in large crustaceans (e.g., Daphnia sp.) and a reduction in A. planktonica densities observed in the months following the P. fluviatilis removal. Only a small proportion of the total P. fluviatilis population was removed and it is unlikely that the effects will be long-lasting without subsequent removal steps. However, it seems likely that P. fluviatilis is one of the factors contributing to cyanobacterial blooms and management of this fish species should be considered in future lake restoration plans. Growth experiments indicated A. planktonica grow over a wide range of light intensities and temperatures, although highest growth rates were generally associated with higher temperatures (25 C) and light intensities (60 - 140 μmol m-2 s-1). Ecological and hydrodynamic trends within the LKR over a two year period were simulated with adequate success using the model DYRESM-CAEDYM. Management scenarios simulated using DYRESM-CAEDYM suggest implementation of an artificial destratification system in the LKR may be the most practical and effective means of controlling A. planktonica blooms. The addition of an artificial aeration system emitting air at a rate of approximately 50 l-1 s-1 should result in an isothermal system. Without summer stratification some of the physiological features of A. planktonica (e.g., buoyancy regulation and nitrogen-fixation) that give it a competitive advantage over other phytoplankton species will be reduced.
20

Spatial Variation of Dissolved Organic Carbon along Streams in Swedish Boreal Catchments

Temnerud, Johan January 2005 (has links)
<p>Denna avhandling behandlar den småskaliga rumsliga variationen av löst organiskt kol (DOC, engelsk term: dissolved organic carbon), dess koncentration, masstransport och karaktär, i bäckar inom två barrskogsbeklädda avrinningsområden (delavrinningsområden 0,01-78 km²). Provtagningen gjordes uppströms och nedströms varje bäckförgrening under sommarbasflöde. Koncentrationen av DOC varierade inom en tiopotens (4-66 mg/l), liksom flera andra kemiska parametrar. Vid riksinventeringen av vattendrag för 2000 i norra Sverige noterades en snarlik spännvidd. Enligt Naturvårdverkets bedömningsgrunder för sjöar och vattendrag återfanns alla tillståndsklasser för DOC och pH, samt mänsklig påverkansgrad till försurningen inom de två studerade områdena. Den specifika avrinningen (flöde per areaenhet) varierade kraftigt i källflödena och inverkade på deras bidrag till kemin nedströms. Vattenkemin och specifika avrinningen var stabila i delavrinningsområden större än 15 km². Det kan vara nödvändigt att provta så stora områden om generella värden för landskapet önskas, men källflödenas kemi kommer då inte att kunna karakteriseras, ehuru källflödena utgör den största delen av bäcksträckan och det akvatiska ekosystemet.</p><p>Nedströms är DOC-koncentrationen, och många andra kemiska parametrar, lika med summan av bidragen från källflödena och nedströms utspädning av inflödande vatten. Tillkommer gör processer inom bäcken och vattnets utbyte med botten/grundvatten (den hyporheiska zonen), men även DOC-förluster/transformationer vid vissa bäckförgreningar och sjöar. Ovanstående resonemang förklarade bäckvattenkemins minskande variation och vanligen lägre DOC-koncentrationer nedströms i ett landskapsperspektiv. Längs bäcksträckorna observerades ingen generell minskning av DOC-koncentration eller ändring av dess karaktär. Skillnaden i DOC-koncentration och relaterade parametrar mellan källflöden och nedströms styrdes i hög grad av vilka landskapselement (myrar, sjöar och skogsmark) som vattnet hade passerat innan det hamnade i bäckarna, samt samspelseffekter med den specifik avrinningen och platserna med DOC-förlust. Prognoser med multivariata modeller baserade på kartinformation testades men kunde inte förutsäga det rumsliga mönstret eller DOC-koncentrationen med erforderlig precision.</p><p>Lämpliga rutiner inom miljöövervakningen för att övervaka den rumsliga variationen av kemin i ytvatten saknas alltjämt. Vid planering av terrestra och akvatiska skötselåtgärder bör man beakta bäckvattnets naturliga kemiska variation.</p> / <p>This thesis quantifies the small-scale spatial variation of dissolved organic carbon (DOC) concentrations, fluxes and character in two boreal catchments (subcatchments 0.01-78 km²) using ”snapshots” of summer base flow where samples were taken upstream and downstream from every node in the stream network. An order of magnitude variation was found in DOC-concentrations, and many other chemical parameters. The range was similar to that found in all of northern Sweden by the national stream survey in 2000. According to the official assessment tools used in Sweden, the entire range of environmental status for DOC, pH and human acidification influence existed within these two study catchments. A large variability in specific discharge had a major impact on the contribution of headwaters to downstream chemistry. The water chemistry parameters were relatively stable at catchment areas greater than 15 km². Sampling at that scale may be adequate if generalised values for the landscape are desired. However the chemistry of headwaters, where much of the stream length and aquatic ecosystem is found would not be characterized.</p><p>Downstream DOC-concentrations, as well as many other chemical parameters, are the sum of headwater inputs, in combination with progressive downstream dilution by inflowing water with its own DOC-concentration and character. Superimposed upon this are in-stream and hyporheic processes, as well as discrete loci of DOC loss/transformation at lakes and stream junctions. At the landscape scale, this results in a decreased downstream variation in stream water chemistry and often, but not necessarily, lower average DOC-concentrations. Along stream reaches there was not a loss of DOC-concentration or a consistent change in character. While the importance of in-stream/hyporheic processes that consistently alter DOC-concentrations along the channel network cannot be ruled out, the differences between headwater and downstream DOC-concentrations and related parameters depend largely on the mosaic of landscape elements (mires, lakes and forest soil) contributing water to the channel network, combined with patterns of specific discharge and discrete loci of DOC loss. Assessment would be facilitated by map information that could predict spatial patterns. Multivariate models using maps, however, did not give satisfactory predictions.</p><p>Appropriate procedures for dealing with spatial variation in the environmental assessment of surface waters are not yet established. An awareness of stream water chemistry’s natural spatial variability should be considered when planning aquatic and terrestrial management.</p>

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