<p>This thesis will investigate the possibilities to wireless communicate within time critical applications with the radio protocol IEEE 802.15.4.</p><p>IEEE 802.15.4 is a very quick protocol so the delays and jitter can be ignored in a small network.</p><p>The thesis handles the question: Is it possible to split a CAN-bus and send the data via radio to the other side of the CAN-bus?</p><p>The big problems with this are:</p><p>• Will it be treated like a transparent link and not disturb the original functionality?</p><p>• How does a system which is split up by radio treat new nodes on the bus?</p><p>• What will be the maximum speed and the maximum utilization factor for the split up bus?</p><p>• What new suitable protocols can be implemented on a higher level to get the split bus to work?</p><p>This is modeled and discussed, real measurement from a radio link is used in the model to see if it’s possible.</p><p>The radio communication will satisfy the demands from the company regarding their application. With an efficient error handling and a smart transmission protocol the application can be a very smart way of sending CAN-data via radio.</p> / <p>Denna rapport undersöker möjligheterna med att kommunicera trådlöst i</p><p>tidskritiska applikationer med hjälp av radioprotokollet IEEE 802.15.4.</p><p>IEEE 802.15.4 är ett väldigt snabbt protokoll så jitter och fördröjningen i sändningarna kan försummas för så små nätverk som tas upp i denna uppsats.</p><p>Går det att bryta en CAN-buss och koppla in denna radiolänk så att den</p><p>uppfattas som transparent?</p><p>Många problem kommer att dyka upp om detta görs, några av dem är dessa:</p><p>• Hur behandlar ett system nya noder som kopplas in om CAN-bussen är uppdelad?</p><p>• Vilken är den maximala hastigheten och utnyttjandefaktor som kan uppnås av den delade CAN-bussen?</p><p>• Måste ett överliggande protokoll användas för att sköta om datatrafiken mellan bussarna?</p><p>Detta modelleras och diskuteras efter det att de uppmätta mätvärdena har samlats in och analyserats.</p><p>Det som framkom var att det fungerar väldigt bra, data kommer fram i tid och felen i radiosändningarna är väldigt små. Med en väl genomtänkt felhantering och ett likaså genomtänkt omsändningsprotokoll kommer detta att fungera väldigt bra beroende på avstånd och antennval.</p>

A new method of connecting cross-frames to bridge girders had been proposed to alleviate concerns with current design practices. This new, half-pipe detail needs to be examined for fatigue issues that may exist which would make it infeasible as a replacement candidate for the current bent-plate design. A program of laboratory testing was carried out to determine the comparative performance between the half-pipe and the bent-plate designs. These tests were then translated into a finite element model which was examined to determine behavior over a wide range of designs scenarios. Finite element results, along with the laboratory testing data, were used to determine the appropriate use of the half-pipe stiffener. / text

Designing a modern processor is a very complex task. Writing the entire design using a hardware description language (like Verilog) is time consuming and difficult to verify. There exists a split architecture/microarchitecture description technique, in which, the description of any hardware can be divided into two orthogonal descriptions: (a) an architectural contract between the user and the implementation, and (b) a microarchitecture which describes the implementation of the architecture. The main aim of this thesis is to build realistic processors using this technique. We have designed an in-order and an out-of-order superscalar processor using the split-description compiler. The backend of this compiler is another contribution of this thesis. / text

No description available.

Proteins are intimately involved in almost every cellular phenomenon, from life to death. Understanding the interactions of proteins with each other and other macromolecules and the ability to rationally redesign them to improve their activities or control their function are of considerable current interest. Split-protein methodologies provide an avenue for achieving many of these goals. Since the original discovery of conditionally activated split-ubiquitin, the field has grown exponentially to include the activities of over a dozen different proteins. The flexibility of the systems has resulted in their use across a wide spectrum, both literally and figuratively, to primarily screen, visualize and quantitate macromolecular interactions in a variety of biological systems. In another arena, there is significant interest the apoptosis-regulating proteins: the Bcl-2 family. These proteins are found in many cell types and control, through expression levels as well as other mechanisms, the apoptotic state of a protein as governed by intrinsic death signals generated from such sources as DNA damage and viral infection. The apoptotic function of these proteins are mainly governed by a single type of interaction: the helix:receptor binding of the BH3-Only helices to the anti-apoptotic receptor proteins. While this often promiscuous helix:receptor interaction has received much scrutiny, the nature of the anti-apoptotic binding pocket, especially with regard to the specific residues that govern the interaction, has been lacking. With the high sensitivity and rapid analysis platform afforded by the cell-free split-luciferase analysis methodology, we devised and carried out the first systematic and large scale alanine mutagenesis of all five major anti-apoptotic members of the Bcl-2 family, validated these results both with biophysical methods as well as correlation with previous studies. Our results help explain how different receptors can bind a wide range of helices and also uncovered details regarding binding that are not possible with structural or computational analysis alone. In a second area of research, we have utilized the interaction of BH3 helices and their receptors for designing small molecule controlled protein kinases and phosphatases. In this protein design area, BH3-Only helices were inserted using a knowledge based approach into particular loops within both a protein kinase and a protein phosphatase. The BH3-Only helix interaction with added receptors, such as Bcl-xL provided an allosteric switch for turning-off the activity of the helix-inserted enzymes. The activity of the enzymes could then be turned-on by the addition of a cell-permeable small molecule that is known to bind the receptor. This plug-and-play design was demonstrated to be successful for two very different enzyme classes and likely provides a general and tunable biological element for controlling the activity of one or more proteins and enzymes in a biochemical networks.

The reversible phosphorylation of proteins plays a key role in nearly every aspect of cell life. This essential post-translational modification controls a myriad of cellular events from cell survival, differentiation, and migration to apoptosis. Two classes of enzymes, kinases and phosphatases, tightly control all phosphorylation events. Perturbation in the activity of any member of these classes of enzymes has been linked to numerous diseases including cancer, metabolic disorders, immune disorders and neurological disorders. Therefore, there is a great interest among the scientific community to develop methods to selectively modulate the activity of kinases and phosphatases not only for therapeutic purposes but also to understand the fundamental role of these enzymes in signaling events. The more than 500 kinases encoded in the human genome share a common catalytic fold and most inhibitors target the ATP binding site. Therefore selective targeting of a single kinase by an inhibitor at the highly conserved ATP binding site is one of the main concerns for designing probes or drugs. Our group has taken advantage of the potency and possible selectivity imparted by bivalent inhibitors and developed an in vitro selection approach to discover bivalent ligands. The strategy involves the use of an ATP-competitive small molecule warhead and a library of cyclic peptides displayed on phage that interact with the kinase of interest in a dynamic selection. The selection for a kinase binding peptide is carried out until consensus peptides are found and bivalent ligands are constructed by linking the selected cyclic peptide with the small molecule warhead through a synthetic linker. Using this approach a potent and selective bivalent inhibitor was found for PKA, a serine/threonine kinase. To interrogate the generality of this approach, a kinase closely related to PKA (PRKX) was used for which a very potent and selective bivalent ligand was found. The same selection strategy was further extended to the two kinases Lyn and Brk, which belong to the tyrosine kinase family. Though peptides were isolated that bound the desired kinase, potent bivalent inhibitors were not discovered. More generally, these experiments in sum are building a library of information regarding how to best design selections of potent and selective bivalent inhibitors. We further explored modulation of the activity of kinases and phosphatases by employing a ligand-gated split-protein approach. The small molecule gated spatial and temporal control of these enzymes should allow the study of signaling events in a controlled manner. The strategy employed consists in the identification of possible fragmentation sites within the catalytic domain of kinases and phosphatases by a sequence dissimilarity approach. Loop insertion mutants at the selected sites were tested for catalytic activity. Successful insertion mutants were further split into two catalytically inactive fragments, which were appended to two conditionally interacting protein domains. Upon addition of a small molecule, the two conditionally interacting domains reassemble the catalytic domain of the enzyme and restore catalytic activity. Using this approach we were able to modulate the activity of the tyrosine kinases Lyn, Fak and Src and the AGC kinase PKA. We also extended the approach to gate the activity of tyrosine phosphatases PTP1B, SHP1 and PTPH1. Finally, these ligand-gated split-kinases and phosphatases were validated in-cellulo. Thus, this work resulted in a new method for designing split-proteins and provided a palette of kinases and phosphatases that can be turned-on by small molecules. In total, these efforts describe two alternative routes that can be used to modulate phosphorylation events in a selective and controlled manner.

This dissertation is an analysis, assuming the framework of Government and Binding Theory, of the syntactic derivation of comparative correlative constructions (hereafter CCs for short) in Mandarin Chinese. It attempts to evaluate the theoretical adequacy of extant treatments of CCs and propose an alternative analysis to the prevailing adjunct approach. CC constructions exist crosslinguistically. An English example is The more chocolate I eat, the happier I feel. In Chinese, a simplex CC sentence consists of two non-coordinated clauses; the lexical word yue, which indicates degree, is obligatory in both clauses, as illustrated in (1): (1) tianqi yue₁ re, dian-fei yue₂ gao. weather [ YUE₁ hot], electricity-fee [YUE₂ high] `The hotter the weather is, the higher the electricity fee is.' Unlike the English comparative phrase, which has been shown to undergo A-bar movement in earlier studies, the yue-constituent remains in situ. I argue that yue is generated in [Spec, DegP] and behaves as an indefinite in-situ degree element on a par with an in-situ wh-element (Li 1992; Tsai 1994; Cheng and Rooryck 2000; Cheng 2003a, 2003b). The yue-variable in each clause is unselectively bound (Lewis 1975, Heim 1982, Cheng and Huang 1996) by an implicit CORRELATIVITY OPERATOR and does not undergo A-bar movement. In addition to the idiosyncratic in-situ yue-phrase, another property of CCs is the syntactic interdependency between the constitutive clauses. Earlier studies (Dikken 2005, Taylor 2006, 2009, Tsao and Hsiao 2002) treat the preceding clause as an adjunct. However, an adjunct approach cannot account for the property of syntactic interdependency. As an alternative, I assume Rizzi's (1997) work on the Split CP Hypothesis, arguing that Chinese CCs implicate the information structure in the left periphery and that they are a type of Focus construction. A Chinese CC sentence like (1) is projected by a null functional head Foc⁰. The first clause is focused and base-generated in [Spec, FocP] and the second clause is the complement of the null Foc⁰. The [+focus]feature in Foc⁰ licenses the co-occurrence of yue₁ and yue₂. This alternative analysis can capture not only crosslinguistic commonalities but also the language-internal property of topic-prominence in Chinese.

Split-screen innebär att filmduken är uppdelad i flera delar. Uppsatsen behandlar filmer där subjektiva upplevelser beskrivs med hjälp av split-screen. Det är ett berättande utifrån en karaktär. En subjektiv upplevelse kan vara en dröm, en hallinucation, ett minne, eller förnimmelse. Split-screen har funnits sen filmens begynnelse och haft sina glansdagar på bland annat 1910-talet och 1970-talet. I och med datorerna är det lättare att skapa split-screeneffekter Vi som publik är också mer vana vid flera datorfönster och bildrutor samtidigt. Split-screen har kommit in i en ny glansdager era. Det finns ett flertal filmer som använder sig av split-screen för att skildra subjektiva upplevelser. Uppsatsens metod är att lyfta fram exempel då det fungerar med split-screen och subjektiva upplevelser.

No description available.

In industrial experimentation, time and material costs are often at a premium. In designing an experiment, one needs to balance the desire for sufficient experimental runs to provide adequate data analysis, with the need to conduct a cost-effective experiment. A common compromise is the use of fractional factorial (FF) designs, in which only a fraction of the total possible runs is utilized. After discussing the basic concepts of FF designs, we introduce the fractional factorial split-plot (FFSP) design. Such designs occur frequently, because certain factors are often harder to vary than others, thus imposing randomization restric- tions. This thesis examines two techniques aimed at reducing run size that have not been greatly explored in the FFSP setting — nonregular designs and semifoldover designs. We show that these designs offer competitive alternatives to the more standard regular and full foldover designs and we produce tables of optimal designs in both scenarios.