Bakgrund: Posttraumatiskt stressyndrom (PTSD) är en av de vanligaste psykiatriska följdsjukdomarna efter en traumatisk upplevelse. Många av de individer som har diagnostiserats med PTSD har samtidiga kroppsliga besvär och det finns forskning som tyder på att fysioterapeutiska interventioner skulle kunna vara betydelsefulla i behandlingen av PTSD. Trots att forskning på området fysioterapi vid PTSD har ökat i omfattning under de senaste åren är fysioterapeutprofessionen inte självklar i PTSD-teamet. En kvalitativ studie om hur fysioterapeuter upplever sin roll i behandlingen av patienter med PTSD skulle kunna bidra med värdefull information. Syfte: Syftet med studien var att beskriva fysioterapeuters upplevelser och erfarenheter av att arbeta med vuxna personer som har diagnostiserats med posttraumatiskt stressyndrom. Metod: Studien var en kvalitativ intervjustudie som baserades på fem semistrukturerade intervjuer med fysioterapeuter från Röda Korsets behandlingscenter i Sverige. Ett ändamålsenligt urval tillämpades i studien och datan analyserades utifrån en kvalitativ innehållsanalys. Resultat: Analysprocessen resulterade i 14 underkategorier och fyra kategorier. De fyra kategorierna var; Ett varierat och individanpassat fysioterapeutiskt arbetssätt vid PTSD, Fysioterapeutens betydelse och unika roll i PTSD-teamet, Variationsrika framgångsfaktorer inom vården av PTSD och Utmaningar som komplicerar vården vid PTSD. Konklusion: Fysioterapeuter har, i och med sin unika kompetens, en betydelsefull roll i PTSD-teamet och kan genom kroppsliga behandlingsmetoder göra en stor skillnad för patienter med PTSD. Utmaningarna för fysioterapeuter inom vården av PTSD var många och det fanns en önskan om mer forskning och utbildning. Vidare forskning på området fysioterapi vid PTSD behövs för att fylla de kunskapsluckor som finns inom området. / Background: Posttraumatic stress disorder (PTSD) is one of the most common psychiatric disorders following a traumatic event. Many of those who have been diagnosed with PTSD have bodily symptoms and research suggests that physiotherapeutic interventions may be meaningful in the treatment of PTSD. Even though research regarding physiotherapy and PTSD has grown over the last couple of years physiotherapists are not a natural part of the PTSD-team. A qualitative study regarding physiotherapists views on their role in the treatment of PTSD could provide valuable information. Purpose: The purpose of this study was to describe physiotherapists' views and experiences of working with adults who have been diagnosed with posttraumatic stress disorder.  Method: The study was a qualitative interview study which was based upon five semi-structured interviews with physiotherapists from the Red Cross treatment centers in Sweden. A purposive sampling was used and the data was analyzed using qualitative content analysis.   Results: The analysis resulted in 14 subcategories and four categories. The four categories were; A varied and personalized physiotherapeutic way of working with PTSD, The physiotherapists importance and unique role in the PTSD-team, Variating successful factors in the healthcare of PTSD and Challenges that complicates the healthcare of PTSD. Conclusion: Physiotherapists have, because of their unique competence, an important role in the PTSD-team and can make a big difference for their patients through physiotherapeutic interventions. There are many challenges in the healthcare of PTSD for physiotherapists and more research and education was requested. Further research regarding physiotherapy and PTSD is needed.

Understanding the neuronal basis of learning and memory is a fundamental problem in neuroscience. A leading theory, the origins of which date back to the beginning of the twentieth century, is that the neural basis for memory resides in engrams (also called memory traces), ensembles of cells that are activated during learning and reactivated during memory retrieval. Recent genetic tools have allowed researchers to visualize and manipulate memory traces in small brain regions; however, the ultimate goal is to analyze memory traces across the entire brain in order to better understand how memories are stored in neural networks and how multiple memories may coexist. In order to do so, methods and technologies need to be developed that allow labeling of engram cells throughout the brain, visualization of these cells, and automated quantification of cells in an anatomically precise manner. The first of these challenges has been addressed through the development over the past several years of different mouse models that permit the labeling of active cells throughout the brain at multiple time points. One of the most powerful models, the ArcCreERT2 mouse line, uses drug-induced genetic recombination to indelibly label cells throughout the brain in an activity-dependent manner. In this thesis, I present our work utilizing this model to solve the second and third challenges: imaging of brain-wide memory traces and automated quantification of labeled cells, as well as the application of these novel methods to understanding the engram network changes following fear extinction. Intact tissue clearing and imaging is a new and rapidly growing area of focus that holds great promise for enabling the brain-wide visualization of memory traces. We utilized the leading protocols for whole-brain clearing and applied them to the ArcCreERT2 mice. We found that CLARITY and passive clarity technique (PACT) greatly distorted the tissue, and immunolabeling-enabled three-dimensional imaging of solvent-cleared organs (iDISCO) quenched enhanced yellow fluorescent protein (eYFP) fluorescence and hindered immunolabeling. Alternative clearing solutions, such as tert-Butanol, circumvented these harmful effects, but still did not permit whole-brain immunolabeling. Clear unobstructed brain imaging cocktails and computational analysis (CUBIC) and CUBIC with Reagent 1A produced improved antibody penetration and preserved eYFP fluorescence, but also did not allow for whole-brain memory trace visualization. We developed CUBIC with Reagent-1A*, a modified CUBIC protocol that resulted in eYFP fluorescence preservation and immunolabeling of the immediate early gene (IEG) Arc in deep brain areas; however, optimized memory trace labeling still required tissue slicing into mm-thick tissue sections. Nonetheless, our data show that CUBIC with Reagent-1A* is the ideal method for reproducible clearing and immunolabeling for the visualization of memory traces in mm-thick tissue sections from ArcCreERT2 brains. Recent developments in brain-wide engram tagging strategies, primarily through the use of transgenic mouse models such as the ArcCreERT2 line, and whole brain imaging strategies, such as CLARITY, CUBIC, and iDISCO, have created the circumstances to, for the first time, be able to visualize throughout the brain neuronal activity that is directly linked to behavior. However, as noted above, quantifying and analyzing these brain-wide memory traces presents its own challenge, and widely applicable, readily accessible solutions to this problem have thus far been limited. Although a handful of freely available programs and suites do exist, such as CellProfiler and ClearMap, these are generally tailored to specific approaches, and in particular, no currently available solution exists for quantifying multi-labeled engram cells imaged in three dimensions along the coronal plane, a relatively common scenario that is sure to become even more prominent as greater adoption of the underlying technologies progresses. Using ImageJ and R, we developed an image analysis pipeline to solve precisely this problem. Our strategy allows for the segmentation of both the encoding and retrieval populations, including identification of the reactivated cells, and registration of segmented cells to an anatomical atlas in order to analyze cell activity in a region- and layer-specific manner. Post-traumatic stress disorder (PTSD) can develop following a traumatic event and results in heightened, inappropriate fear and anxiety. Approximately 8% of the US population suffers from PTSD, the main treatment for which is repeated exposure to triggering stimuli under controlled conditions. A better understanding of the neural circuits modified during this process would help advance therapeutic treatment for PTSD. We sought to determine the brain-wide neuronal activity changes underlying fear extinction, the best laboratory model of exposure therapy, by using the ArcCreERT2 x eYFP mice and our newly developed brain-wide segmentation and registration pipeline. ArcCreERT2 x eYFP mice were administered a 4-shock contextual fear conditioning (CFC) paradigm followed by either a 10-day extinction protocol or re-exposure to the aversive context without extinction. Following the final exposure session, mice were euthanized, and active cells were quantified throughout the brain using the pipeline. We found that fear learning leads to increased functional connectivity of amygdalar and hypothalamic regions, and extinction leads to a decentralization of the fear memory network and disengages the thalamus and striatal amygdala. Additionally, coordinated reactivation of the basomedial amygdala and secondary somatosensory cortex with frontal association regions are differentially modulated following extinction, and we identified the temporal association area and medial habenula as novel brain regions involved in modulating freezing behavior. In summary, in this thesis, we have developed a novel engram analysis pipeline and shown its potential for quantifying brain-wide memory traces. This is the first study to analyze brain-wide functional connectivity following fear learning and extinction of a recent fear memory, as well as the first study to analyze fear memory trace reactivation patterns across the brain and relate all three measures to behavioral output. This work both greatly enhances our understanding of the neural underpinnings of fear extinction and provides a toolset for readily exploring the neural underpinnings of other behaviors and types of associative memory.

We investigated the effects of the Gulf War on two groups of Kuwaiti children and youth. Those children experiencing war-related traumatic events and those who did not experience or witness a war-related traumatic event participated. Data were obtained from the mother of each child. Each mother completed a structured Interview which Included Child Traumatic Reaction Index, developed according to the DSM-IV (1994) criteria for Post Traumatic Stress Disorder (PTSD). The data indicated a significant interaction effect observed by mothers according to the child's gender, experience of war traumatic events, and PTSD. The results are discussed In terms of the diagnostic criteria for PTSD, the necessity of early Identification, and the difficulty associated with the diagnosing PTSD in children.

Recent studies of children and adolescents who have experienced a residential, industrial, or wild fire have suggested a causal link between fire disaster and PTSD related psychological distress. Not everyone, however, is equally affected by the stress of experiencing such an event, and the role of coping in this process may be an important mediating factor. Additionally, several studies have found that girls and African Americans report more distress following disasters than do boys and Caucasians. The current study sought to investigate the roles of exposure/loss, coping efficacy, and coping strategy in mediating psychological distress in adolescents after a disaster. The current study included a representative sample of 206 9th graders from a Central Florida High School affected by severe wildfires who were assessed via self-report measures 3- and 10- months after the fires, in a latent variable path analysis to assess the fit of a model including exposure/loss, coping efficacy, coping strategy, and PTSD, depression, and anxiety scores. Moreover, acculturation level and SES were included along with gender and ethnicity in testing for the moderating role of sociodemographics, as little research has delved into the important proximal factors affecting reported racial differences, as ethnicity is better conceptualized as a distal variable that works through a variety of proximal variables to affect outcomes. Results indicated that although the assessment of the global fit of the latent variable path model revealed it to be a poor fit to the data, component fit of the model pointed to a possible mediating role of coping efficacy between exposure/loss and psychological distress, as well as coping efficacy being associated negatively with avoidant coping strategies. Likewise, post hoc regression analyses indicated an important role for exposure/loss, coping efficacy, and coping strategy as they related to PTSD symptomatology in adolescents at both Time 1 and Time 2. Finally, although relationships between the proposed variables and PTSD did not interact with gender, acculturation, SES, or ethnicity, there was a significant interaction between acculturation and ethnicity signifying that for African American youth, high acculturation levels were predictive of less PTSD symptomatology. / Ph. D.

We present an evidence-based review of post-trauma interventions used to prevent posttraumatic stress disorder (PTSD). Literature search of PubMed from 1988 to March 2016 using keywords “Early Intervention AND Prevention of PTSD” yielded 142 articles, of which 52 intervention studies and 6 meta-analyses were included in our review. Trauma-focused cognitive behavioral therapy and modified prolonged exposure delivered within weeks of a potentially traumatic event for people showing signs of distress have the most evidence in the treatment of acute stress and early PTSD symptoms, and the prevention of PTSD. Even though several pharmacological agents have been tried, only hydrocortisone prior to high-risk surgery, severe traumatic injury, or during acute sepsis has adequate evidence for effectiveness in the reduction of acute stress symptoms and prevention of PTSD. There is an urgent need to determine the best targets for interventions after trauma to accelerate recovery and prevent PTSD.

Background The postpartum period represents a major transition in the lives of many women, a time when women are at increased risk for the emergence of psychopathology including depression and PTSD. The current study aimed to better understand the unique contributions of clinically significant postpartum depression, PTSD, and comorbid PTSD/depression on mother–infant bonding and observed maternal parenting behaviors (i.e., behavioral sensitivity, negative affect, positive affect) at 6 months postpartum. Methods Mothers (n=164; oversampled for history of childhood maltreatment given parent study's focus on perinatal mental health in women with trauma histories) and infants participated in 6-month home visit during which dyads engaged in interactional tasks varying in level of difficulties. Mothers also reported on their childhood abuse histories, current depression/PTSD symptoms, and bonding with the infant using standardized and validated instruments. Results Mothers with clinically significant depression had the most parenting impairment (self-report and observed). Mothers with clinically significant PTSD alone (due to interpersonal trauma that occurred predominately in childhood) showed similar interactive behaviors to those who were healthy controls or trauma-exposed but resilient (i.e., no postpartum psychopathology). Childhood maltreatment in the absence of postpartum psychopathology did not infer parenting risk. Limitations Findings are limited by (1) small cell sizes per clinical group, limiting power, (2) sample size and sample demographics prohibited examination of third variables that might also impact parenting (e.g., income, education), (3) self-report of symptoms rather than use of psychiatric interviews. Conclusions Findings show that in the context of child abuse history and/or current PTSD, clinically significant maternal depression was the most salient factor during infancy that was associated with parenting impairment at this level of analysis.

There is growing evidence to suggest an inverse association between physical activity and symptoms of posttraumatic stress disorder (PTSD). However, the mechanisms are not well understood and much of the research in this area stems from cross-sectional studies, thereby limiting what is known about these relationships at the intra-individual level. Chapter 1 of this dissertation is a systematic review examining the literature on the association between physical activity and PTSD in a variety of study designs (i.e., cross-sectional, longitudinal, and intervention). Chapters 2 and 3 used data from a 7-day diary study of 15 participants with a diagnosis of PTSD. In this study, participants completed twice daily surveys on mobile phones and wore Fitbit accelerometers measuring physical activity and sleep. Chapter 2 used multilevel modeling to examine the within-person and between-person associations between physical activity and symptoms of PTSD, sleep, positive and negative affect, and coping. Multiple operationalizations of physical activity were used (i.e., self-report and accelerometer-measured) in order to explore and better understand which metrics are most strongly related to psychosocial factors. Results from Chapter 2 add to the literature by providing evidence of within-person associations between physical activity and PTSD symptoms over the course of the day, such that on days when participants are more physically active than usual, they also report fewer symptoms of PTSD that evening. Chapter 3 discusses the utility of using N-of-1 study designs with an emphasis on the benefits of using frequent repeated measurements in clinical practice. Three case examples are presented to illustrate the intra-individual variability that is observed in symptoms of PTSD, affect, and health behaviours. These examples provide rationale for the use of intensive measurement designs in order to fully capture and understand how and when variables fluctuate over time. / Graduate

Veterans with post-traumatic stress disorder (PTSD) have increased rates of overweight/obesity, higher prevalence of obesity-related medical conditions, and greater morbidity and mortality compared to their peers without PTSD. The aim of this study was to assess the impact of PTSD on weight and metabolic parameters as well as the effect of weight change on metabolic indices. In a case-control cohort, we compared PTSD patients (n=364) with those without mental health disorders (NoMH, n=1,008) at baseline (i.e., eight years prior to enrollment) and on the trajectory of changes before the enrollment in the Miami VA MOVE!® (Weight Management for Veterans) program. Multilevel modeling for change was used to investigate changes in weight and physiological markers over time. The sample consisted of 1,372 veterans (1,208 men and 164 women). Mental health status, weight and metabolic parameters were obtained from medical record. The PTSD group was significantly younger, had lower average BMI at baseline, and higher prevalence of weight-related medical conditions. Groups did not differ by gender, race/ethnicity, or Operation Enduring Freedom/Operation Iraqi Freedom (OEF/OIF) status. At baseline, the PTSD group had significantly lower weight, lower HDL-cholesterol, and higher values for glucose and triglycerides than the NoMH group. The PTSD group had a significantly greater slope than NoMH group for HDL-cholesterol (p¬=.011). The difference in rate of change between groups for weight, glucose, and triglycerides was not significant. The sample as a whole showed a significant increase for weight and glucose while total cholesterol, blood pressure, and triglycerides decreased over time. Despite lower weight, veterans with PTSD had greater prevalence of weight related-medical conditions while rate of change in metabolic parameters over time was comparable to NoMH peers. Exploratory analyses showed that weight had a significant negative effect on total cholesterol and blood pressure. PTSD appears to be a significant risk factor for diabetes and overall metabolic dysfunction. The lack of results with regards to the effect of weight on HDL cholesterol, triglycerides, glucose and hemoglobin A1c are not surprising given the complex relationships among weight and these metabolic indices.

Indiana University-Purdue University Indianapolis (IUPUI) / Intimate partner violence is a problem in the United States (U.S.) military. Previous research has identified factors that increase a couple’s risk for engaging in violence. Most of these factors, such as age, alcohol, and relationship satisfaction are consistent across civilian and military samples. One factor that is unique to military samples is deployment; service members can be exposed to unique traumatic incidents while deployed which are generally unknown to most civilians. Deployments can also increase a service member’s risk for developing Post Traumatic Stress Disorder (PTSD), which can increase their risk for intimate partner violence. Previous research on the effect of deployments on intimate partner violence has produced mixed results. The purpose of this study was to analyze if deployment, total length of deployment, combat experiences from deployment, and PTSD symptoms increased risk for Air Force airmen to perpetrate intimate partner violence at a moderate or severe level of violence. Survey data from a representative sample of active duty Air Force airmen (N = 1,501) was used to conduct Chi-Square analyses and multinomial logistic regression models for perpetrated violence. Results of the study showed that deployment and combat experiences were not significant predictors of perpetrated violence. PTSD symptoms, in addition to alcohol misuse and relationship satisfaction, were significant predictors of moderate and severe perpetrated violence. In terms of practice and policy implications, the study results underscore the importance of widespread screening for these risk factors as well as the availability of interventions focused on alcohol use and relationship issues among service members. Future research could determine if PTSD symptoms moderate the relationship between combat experiences and intimate partner violence.

Thesis is submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Faculty of Humanities, University of the Witwatersrand, Johannesburg, 1998 / The study was directed at exploring the social and personal construction of meaning of male victims of violent crime. Three inter-related goals were identified: the extension of the nascent narrative/thematic tradition of analysis in the traumatic stress field; the deepening of appreciation of the experience of criminal victimization; and the exploration o f the role of social construction in the cognitive schematic adjustment of victims. In this instance the examination of the role of social construction was focussed centrally on the juxtaposition of the subject roles associated with masculinity and victimization. On the basis of both clinical observation and theory, it was postulated that aspects of masculine identification would create difficulties for men attempting to adjust to involuntary exposure to a context in which they were clearly victimized. The thesis provides comprehensive coverage of theory straddling cognitive adjustment to psychological trauma, mainstream and critical social psychological theory on victimization, developmental and descriptive dimensions of theory on masculinity and a range of literature on criminal victimization. The theoretical conceptualization underpinning the study offers a unique integration of this body of knowledge. In order to investigate the research question, ten adult, white, English speaking,South African men who had experienced a life-threatening, criminally motivated attack were interviewed about the event and their subsequent responses. The study was located within the qualitative research tradition allowing for the development of theory, and fox depth of description and interpretation. Semi-structured interviews were recorded and tnmscribdl w batim . Interview transcripts were subjected to a thematic content analysis by means of a computer-aided text interpretation. Frequency figures were calculated, but the emphasis of the analysis was in the area of descriptive and interpretive dimensions. Three central themes emerged as signi fi cant; those of Control, Disillusionment and Anger. Multiple facets of each theme are addressed, including centrally the interface between thematic dimensions and the social construction of masculinity. A meta-theoretical discourse analytic commentary is provided, addressing for example, the rob of the “hero” discourse in interviewee’s reports. In addition, the clinical implications of the data are explored. The study provides evidence of the inter-relationship between social and personal construction o f meaning in the aftermath of tramatization. It is suggested that in the context of victimization the social construction of masculinity is both more intractable and more permeable than might be supposed.