Abstract not available

Thesis (Ph. D.)--York University, 1999. Graduate Programme in Biology. / Typescript. Includes bibliographical references. Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNQ56221.

Smyd1 is the founder of a gene family whose members contain split SET and MYND Domains. Smyd1 has several SET dependent lysine methyl-transferase substrates, including multiple histone lysines and at least one non-histone protein, skNAC. The MYND domain of Smyd1 is required for protein interactions, such as that with skNAC. Conventional Smyd1 knockouts die at E10 due to cardiac defects, including an enrichment of cardiac jelly, a decrease in trabeculation, and the loss of ventricular septation. dHand, a transcription factor specific for right ventricular development, and Irx4, a ventricle specific gene, are down regulated. I have shown that an approximately one kb stretch of DNA sequence upstream of the muscle specific first exon of Smyd1 is sufficient to drive expression of a reporter in transgenic mice. Cardiac specific expression is mediated by a proximal Mef2 binding site whereas skeletal muscle expression is dependent on E-boxes. I have fully analyzed this stretch of sequence via computational methods and made predictions on other potential regulatory factors. Through the use of Cre mediated conditional knockouts, I have shown that the phenotype of the conventional knockout was not due to the introduction of the Neomycin cassette at the gene locus or due to cell non-autonomous effects on the heart. Smyd1 is not only essential for cardiac septation, but throughout embryonic cardiac development, during embryonic skeletal muscle development, and in adult cardiac tissue. Conditionally deficient Smyd1 embryonic hearts are less affected than conventional Smyd1 knockouts, but are embryonically lethal and show poor trabeculation, cardiac hemorrhaging, and a pericardial edema. I detail that the Nkx2.5-Cre mediated Smyd1 deletion phenocopies the skNAC conventional knockout and that both knockouts have similar changes in the expression levels of several genes. Furthermore, when Smyd1 is conditionally removed from adult cardiac tissue, survival rates are diminished. Surprisingly a skeletal muscle specific CKO of Smyd1 mediated by Myogenin-Cre has resulted in perinatal lethality, with a visible phenotype as early as E15. Evident in the phenotype is a large edema between the epithelium and skeletal muscle, fewer myoblasts, decreased muscle mass, increased degenerating cells, and a potentially defective differentiation process. / text

published_or_final_version / abstract / toc / Physiology / Master / Master of Philosophy

Thesis (Ph.D.)--University of Delaware, 2007. / Principal faculty advisors: John F. Rabolt and D. Bruce Chase, Dept. of Materials Science & Engineering. Includes bibliographical references.

Resumo: Objetivos: avaliar as alterações morfológicas das fibras musculares estriadas tipos I e II da uretra de ratas prenhes diabéticas submetidas à cesárea. Métodos: Foram avaliadas 20 ratas Wistar distribuídas em quatro grupos: virgem, prenhe, diabético virgem e prenhe diabético. Os três primeiros grupos foram estudados para servir como controle do grupo principal, o prenhe diabético. O diabete foi induzido com streptozotocin na dose de 40mg/kg de peso corpóreo. O critério de inclusão foi uma glicemia acima de 200mg/dL. No final do experimento, as ratas foram anestesiadas e eutanasiadas para realização da laparotomia exploratória. A vagina e a uretra foram retiradas em monobloco, congeladas em nitrogênio líquido e mantidas a -80°C. O bloco foi submetido a cortes em criostato (6 μm de espessura). As lâminas foram coradas por H&E e utilizados anticorpos anti-miosina lenta e rápida para tipagem das fibras. Foi realizada análise morfológica e semi-quantitativa dos quatro grupos. Resultados: O músculo estriado uretral do grupo prenhe diabético apresentou: adelgaçamento, atrofia, desorganização e rompimento associado à perda de localização anatômica normal das fibras rápidas e lentas e diminuição na proporção de fibras rápidas. Conclusões: Este estudo sugere que o binômio diabete e prenhez danificou o músculo estriado uretral e alterou a composição e a distribuição das fibras tipo I e II . / Abstract: Urinary incontinence (UI) in women is defined as any involuntary urine loss. It is a frequent condition of high economic cost to the government that also results in women's physical, psychological and social damage and impaired quality of life. Various risk factors are involved in UI development; however, association with Diabetes mellitus (DM) is of great interest at present. DM affects multiple organ systems, including the urinary system in approximately 52% of diabetic patients and in those showing only hyperglycemia; however, the association between gestational DM and UI has not been fully explained. Health care professionals must be attentive to this new parameter and attempt to analyze it in more detail so that prophylactic and therapeutic measures can be established. It is necessary to delineate the chronology of the relationship between gestational Diabetes mellitus and vesical complications, the relationship between controlled diabetes and the incidence of incontinence as well as effective treatment modalities for diabetic patients with symptoms in the lower urinary tract. / Orientador: Marilza Vieira Cunha Rudge / Coorientador: Angélica Mércia Pascon Barbosa / Coorientador: Selma Maria Michelin Matheus / Banca: Débora Cristina Damasceno / Banca: Jorge Milhen Haddad / Mestre

Made available in DSpace on 2014-06-11T19:29:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-02-25Bitstream added on 2014-06-13T18:39:54Z : No. of bitstreams: 1 marini_g_me_botfm.pdf: 541869 bytes, checksum: 5d00927490e16293300df917e946209a (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Objetivos: avaliar as alterações morfológicas das fibras musculares estriadas tipos I e II da uretra de ratas prenhes diabéticas submetidas à cesárea. Métodos: Foram avaliadas 20 ratas Wistar distribuídas em quatro grupos: virgem, prenhe, diabético virgem e prenhe diabético. Os três primeiros grupos foram estudados para servir como controle do grupo principal, o prenhe diabético. O diabete foi induzido com streptozotocin na dose de 40mg/kg de peso corpóreo. O critério de inclusão foi uma glicemia acima de 200mg/dL. No final do experimento, as ratas foram anestesiadas e eutanasiadas para realização da laparotomia exploratória. A vagina e a uretra foram retiradas em monobloco, congeladas em nitrogênio líquido e mantidas a -80°C. O bloco foi submetido a cortes em criostato (6 μm de espessura). As lâminas foram coradas por H&E e utilizados anticorpos anti-miosina lenta e rápida para tipagem das fibras. Foi realizada análise morfológica e semi-quantitativa dos quatro grupos. Resultados: O músculo estriado uretral do grupo prenhe diabético apresentou: adelgaçamento, atrofia, desorganização e rompimento associado à perda de localização anatômica normal das fibras rápidas e lentas e diminuição na proporção de fibras rápidas. Conclusões: Este estudo sugere que o binômio diabete e prenhez danificou o músculo estriado uretral e alterou a composição e a distribuição das fibras tipo I e II . / Urinary incontinence (UI) in women is defined as any involuntary urine loss. It is a frequent condition of high economic cost to the government that also results in women’s physical, psychological and social damage and impaired quality of life. Various risk factors are involved in UI development; however, association with Diabetes mellitus (DM) is of great interest at present. DM affects multiple organ systems, including the urinary system in approximately 52% of diabetic patients and in those showing only hyperglycemia; however, the association between gestational DM and UI has not been fully explained. Health care professionals must be attentive to this new parameter and attempt to analyze it in more detail so that prophylactic and therapeutic measures can be established. It is necessary to delineate the chronology of the relationship between gestational Diabetes mellitus and vesical complications, the relationship between controlled diabetes and the incidence of incontinence as well as effective treatment modalities for diabetic patients with symptoms in the lower urinary tract.

Thesis (Ph. D.)--University of Oregon, 2008. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 151-168). Also available online in Scholars' Bank; and in ProQuest, free to University of Oregon users.

Thesis (M.S.)--School of Bioengineering, Georgia Institute of Technology, 2005. Directed by Thomas Burkholder. / Thomas Burkholder, Committee Chair ; Cheng Zhu, Committee Member ; Grace Pavlath, Committee Member. Includes bibliographical references.

No description available.