The solvent-solute interface of a biomolecule is a dynamic but yet highly structured domain that links a chemically diverse solute surface to the chemically homogeneous bulk aqueous phase. The role of the resulting intermediate domain, i.e. the "hydration shell", in regulating DNA structure and recognition has been addressed here by time-resolved infrared spectroscopy. A highly reproducible automated hydration pulse regime was established and implemented for attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy to monitor the structural response of DNA to an incremental growth of its hydration shell on its intrinsic time scale of seconds. The transition from the crystallographically defined BI to the BII substate of B-DNA was found to be driven by the increase of water disorder upon growth of the hydration shell, derived from the water OH-stretching absorption frequency and band width changes. 2D correlation analysis was used to identify different water clusters from the temporal behaviour of their water OH stretching frequencies. The results show that BII-stabilizing structural constraints are exerted by strong water-DNA H-bonds in the grooves of B-DNA and are relieved when the groove-bound water merges into a contiguous hydration shell with the less H-bonded PO2- -solvation sphere at ~14 water molecules per DNA phosphate. The H-bond imbalance at the disjunct hydration sites is split symmetrically around the average H-bond strength of bulk water. Thus, merging into a contiguous hydration shell proceeds at little enthalpic cost and homogeneous connectivity to the outer bulk-like H-bond network, such that alteration in the network distant from the DNA can regulate the BI-BII transition in a cooperative manner. The water connectivity is disrupted by DNA-binding peptides. Remarkably, the data show that the replacement of hydration shell water upon ligand biding is crucial in conferring substate specific recognition by peptides that have little intrinsic structural preference. The antibacterial peptide indolicidin secreted from bovine neutrophils dehydrates the non-PO2--bound hydration sites, thereby rendering the unstructured peptide highly specific for the BI state with vibrational signature almost identical to the bacterial minor groove binder netropsin. The proposed dominant role of hydration shell water for DNA conformation was challenged by studying the competing effect of structured water in the coordination-shell of the lanthanide Eu3+ on water structure in the DNA hydration shell. Whereas no effect is seen at low hydration, a hydrogen-like phase is formed at a stoichiometric ratio of Eu3+ :DNA:H2O of 1:10:140, characterized by a strong increase of the molar volume of hydration water. This novel phase appears attractive for lanthanide and possibly actine separation approaches based on biomolecular coordination.

The solvent-solute interface of a biomolecule is a dynamic but yet highly structured domain that links a chemically diverse solute surface to the chemically homogeneous bulk aqueous phase. The role of the resulting intermediate domain, i.e. the "hydration shell", in regulating DNA structure and recognition has been addressed here by time-resolved infrared spectroscopy. A highly reproducible automated hydration pulse regime was established and implemented for attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy to monitor the structural response of DNA to an incremental growth of its hydration shell on its intrinsic time scale of seconds. The transition from the crystallographically defined BI to the BII substate of B-DNA was found to be driven by the increase of water disorder upon growth of the hydration shell, derived from the water OH-stretching absorption frequency and band width changes. 2D correlation analysis was used to identify different water clusters from the temporal behaviour of their water OH stretching frequencies. The results show that BII-stabilizing structural constraints are exerted by strong water-DNA H-bonds in the grooves of B-DNA and are relieved when the groove-bound water merges into a contiguous hydration shell with the less H-bonded PO2- -solvation sphere at ~14 water molecules per DNA phosphate. The H-bond imbalance at the disjunct hydration sites is split symmetrically around the average H-bond strength of bulk water. Thus, merging into a contiguous hydration shell proceeds at little enthalpic cost and homogeneous connectivity to the outer bulk-like H-bond network, such that alteration in the network distant from the DNA can regulate the BI-BII transition in a cooperative manner. The water connectivity is disrupted by DNA-binding peptides. Remarkably, the data show that the replacement of hydration shell water upon ligand biding is crucial in conferring substate specific recognition by peptides that have little intrinsic structural preference. The antibacterial peptide indolicidin secreted from bovine neutrophils dehydrates the non-PO2--bound hydration sites, thereby rendering the unstructured peptide highly specific for the BI state with vibrational signature almost identical to the bacterial minor groove binder netropsin. The proposed dominant role of hydration shell water for DNA conformation was challenged by studying the competing effect of structured water in the coordination-shell of the lanthanide Eu3+ on water structure in the DNA hydration shell. Whereas no effect is seen at low hydration, a hydrogen-like phase is formed at a stoichiometric ratio of Eu3+ :DNA:H2O of 1:10:140, characterized by a strong increase of the molar volume of hydration water. This novel phase appears attractive for lanthanide and possibly actine separation approaches based on biomolecular coordination.

This thesis offers a comparative historical analysis of sub-state violent contention in Quebec and Corsica. It focuses specifically on the Front de Libération du Québec (FLQ) and the Fronte di Liberazione Naziunale Corsu (FLNC), in 1963 to 1971 and 1976 to 1990, respectively. The thesis argues that the FLQ and the FLNC sought to extend radical ideological space to promote independence in order to achieve revolutionary social and economic change through campaigns of violence and kidnappings. Theoretically, the thesis draws on the contentious politics and social movements literatures, which it notably combines with Radical Flank Effect (RFE). RFEs are interactive processes that aim to map the beneficial and/or detrimental impact of radical group action on moderate groups. Whilst commonly used to understand the political outcomes of social movements, RFE is used in this thesis in conjunction with social movement literature to compare the relationship between these violent movements and their more moderate opponents. To understand the internal dynamics of these movements, I have identified four key elements of contrast: membership, ideology, network structure and strategy. I draw on, for example, McAdam, Tarrow and Tilly's (2001) mobilization method, which aids an understanding of membership and ideology by framing the interaction amongst challengers, their opponents and the media. This thesis seeks to understand FLQ and FLNC mobilization in light of the aim to shape and develop radical ideological space in the sub-states of Quebec and Corsica. It draws on an extensive study of archival data that includes police reports that have only recently been made available in Canada, transcripts of court cases, newspapers, and an interview with a former member of the FLNC, as well as secondary sources. The central orienting question is: what explains the contrasting patterns of sub-state violent contention in Quebec and Corsica? More specifically, why did the FLQ dissolve in 1971, yet the FLNC continued its violent trajectory, albeit less political and nationalist, until 2014? The FLQ and the FLNC differently subscribed to Marxism and postcolonialism. The FLQ was committed to a Marxist program of revolutionary change, and this commitment was shared by the FLNC until the collapse of communism in central and Eastern Europe in 1989. FLQ members considered themselves 'urban revolutionaries' and employed Marxism to understand the economic disparity in industrial Montreal. Early Corsican violent contention, in contrast, included Maoist influences, in particular, through their demand for agrarian reform. The two groups viewed the relationship between their sub-states (Quebec and Corsica) and central states (Canada and France) through a colonial lens, and understood their mobilization against these states and elite minorities (the Anglophone elite in Quebec and the pieds noirs in Corsica) in this light. Both violent movements targeted this colonial relationship. Both the FLQ and FLNC manifestos were economically and politically focused, land and culture were additionally highlighted by the FLNC. This thesis found that sub-state violent contention in the very different contexts of Quebec and Corsica shared an overall pattern, an arc of violent mobilization. The initial mobilization developed from a frustration with moderate political groups; radicalization grew and new tactics were embraced; until turning points that included the assassination of Pierre Laporte by the FLQ and the division of the FLNC into competitive factions, and then a decline of activity, mobilization and recruitment. Although the FLQ and the FLNC contrasted greatly in terms of membership, ideology, organization and strategy, both groups attempted to extend radical space through the use of violent contention in these two very different nations. Ultimately, however, while the FLQ and the FLNC were able to extend or maintain radical space at times, yet they failed to sustain the extension of ideological radical space on the basis on their revolutionary manifestos.

Homogene Immunoassays sind immunologische Testverfahren, bei deren Durchführung vollständig auf Separations- und Waschschritte verzichtet werden kann. Der Substrate Channeling Immunoassay beruht auf der Weitergabe eines Substrates in einem immunologischen Komplex aus zwei Enzymen. Das Produkt des ersten Enzyms dient dem zweiten Enzym als Substrat zur Generierung eines photometrisch nachweisbaren Produktes. Voraussetzung für diese Weitergabe ist die enge räumliche Nähe beider Enzyme. Diese Nähe wird durch eine Bindung zwischen Analyt und anti-Analyt Antikörper vermittelt. Ein solcher Substrate Channeling Immunoassay wurde unter Verwendung der Enzyme Glucoseoxidase und Peroxidase aufgebaut. Das so etablierte System war funktionstüchtig, jedoch blieb seine Sensitivität hinter der normaler, heterogener Immunoassays zurück. Die Grundlage eines Fluorescence Quenching Immunoassays ist der gegenseitige Ausschluß zweier Antikörper bei der Bindung eines Dihapten-Konjugates. Das Konjugat besteht dabei aus dem Analyten und einem Fluorophor. Die beiden um die Konjugatbindung konkurrierenden Antikörper sind ein anti-Analyt Antikörper und ein anti-Fluorophor Antikörper, der zudem über die Eigenschaft verfügt, bei Bindung des Fluorophors dessen Fluoreszenz zu löschen. Externe Gaben des freien Analyten verschieben das eingestellte Gleichgewicht in Richtung Fluorophor-Bindung und damit Fluoreszenz-Löschung. Die Änderung der Fluoreszenz ist direkt an die Konzentration des freien Analyten gekoppelt und dient zu deren Bestimmung. Ein solcher Fluorescence Quenching Immunoassays wurde für die Konzentrationsbestimmung des Herbizides Diuron etabliert. Die erreichten Sensitivitäten erlauben die praktische, immundiagnostische Anwendung des Systems. Ein Dihapten-Konjugat wurde ebenfalls zum Aufbau eines Verfahrens zur Selektion Antikörper produzierender Zellen eingesetzt. Die Selektion der Antikörper produzierenden Zellen erfolgt unter Verwendung eines Toxinkonjugates. Dieses Konjugat besteht aus einem Liganden und einem Toxin. Die Antikörperbindung des Liganden behindert sterisch die Wechselwirkung der Toxinkomponente im Konjugat mit deren Zielstruktur in oder auf der Zelle. Nur Zellen die einen geeigneten Antikörper sezernieren, überleben die Selektion und reichern sich in der Kultur an. Das Selektionsverfahren wurde erfolgreich für die Selektion von E.coli Zellen eingesetzt, die einen rekombinanten, Fluorescein bindenden Antikörper produzierten. Das hierfür synthetisierte Toxinkonjugat bestand aus Fluorescein (Ligand) und Ampicillin (Toxinkomponente). Eine Ablösung der bisher für diese Aufgabe gebräuchlichen, außerordentlich kostenintensiven, Screening Methoden wird damit möglich. / Homogeneous immunoassays are test systems which do not depend on separation steps. The substrate channeling immunoassay is based on the product/substrate transfer in an immunological complex built up by two enzymes. The product of the first enzyme functions as substrate for the second enzyme. The second enzyme generates a photometrically detectable product. The close proximity of these two enzymes is the basis of the substrate channeling. This proximity is created by antibody binding to the corresponding analyte. The enzymes glucose oxidase and peroxidase were used for the development of such an assay system. The established homogeneous immunoassay was functional. But the sensitivity of the assay was much lower than that of conventional heterogeneous immunoassays. The principle of a fluorescence quenching immunoassay is based on the fact that two antibodies exclude each other from binding to a dihapten conjugate. The conjugate consists of the analyte and the fluorophore. The two antibodies which compete for the conjugate binding are an anti-analyte antibody and an anti-fluorophore antibody. This anti-fluorophore antibody quenches the fluorescence of the fluorophore after binding. The addition of free analyte alters the equilibrium of the system so that the anti-fluorophore antibody is bound to the fluorophore and the fluorescence is quenched. The change in fluorescence is therefore an indicator of the concentration of free analyte added. A homogeneous fluorescence quenching immunoassay was established for the determination of the herbicide diuron. The sensitivities obtained allow the practical immunodiagnostic application of the system. A dihapten conjugate was also employed for the development of a selection method for antibody-producing cells. Toxin conjugates were used in this system. Each conjugate consisted of a ligand and a toxin. Antibody binding to the ligand sterically inhibits the toxin component to interact with its target structure. Only cells secreting a binding antibody will survive the selection and will accumulate in culture. The system was applied to the selection of E.coli cells producing a recombinant fluorescein-binding antibody. The toxin conjugate used in experiment consisted of fluorescein (ligand) and ampicillin (toxin component). This selection procedure allowed the isolation of recombinant antibody-producing E.coli cells. It has the potential to replace the time-consuming and labour-intensive methods used so far.

The aim of this diploma thesis is to provide a comprehensive overview of the economic part diplomacy of the Czech Republic and by comparing the approaches of the individual regions to identify possible examples of good practice that could be transferred to other regions and thus help to boost their further development in terms of economic paradiplomacy. The paper provides answers to the questions: why did the Czech regions involve in international relations? What regional instruments do they use to promote their interests? What goals and motives do the regions follow from their involvement in foreign affairs? What are the relationships between each region and the central level? Are the objectives of economic paradiplomacy consistent or inconsistent with the objectives of central government? The work is divided into four parts. The first part shows the key moments in the field of studies in paradiplomacy and presents the research framework of the diploma thesis. The second part is the analysis of strategic documents in terms of the objectives of economic paradiplomacy. The third part contains an analysis of the activities carried out by the regions in the framework of the economic paradiplomacy, by characterizing the cooperation with the central level and the regional actors and by analyzing the tools used by the regions between 2012-2016. The fourth, the final part, then, presents an assessment of economic paradiplomacy and provide answers to research questions.

L’action extérieure du Parlement écossais est l’objet de notre étude. D’abord, nous avons identifié la capacité opérationnelle de cette institution au sein du système institutionnel britannique, sur la scène communautaire et dans les échanges internationaux. Puis, nous avons détecté les facteurs structurant cette action. Selon notre étude, trois pôles prennent forme dans l’action parlementaire tels que les îles Britanniques, l’Europe qui couvre l’espace géographique européen, l’environnement institutionnel communautaire. Enfin, le troisième pôle est l’espace hors d’Europe, notamment les pays du Commonwealth et les Etats-Unis d’Amérique. Nous avons également établi une certaine spécialisation des méthodes et des moyens d’action dans les trois pôles évoqués. / The focus of my dissertation is the external action of the Scottish Parliament. My study identifies the operational capacity of this institution within the British institutional system, on the European Union arena and in international relations. I have identified the factors structuring the parliamentary action that shaped three poles such as the British Isles, Europe and outside the geographic European space. The pole of Europe covers both Continental Europe and the EC institutional environment. I have also identified the specialisation of methods and tools of action within the above mentioned poles

The following dissertation develops a theoretical framework for guiding the strategy of democratic states in successfully countering the hostilities of nationalistic substate terrorist organizations (NSTOs), and effectively manipulating the terrorist group’s (and its supporting elements’) decision-making calculus. In particular, the theory of strategic coercion has been chosen as a basis for formulating this framework, based upon: 1) the invaluable guidance it offers in dynamically drawing upon all instruments of national power—economic, diplomatic, military, etc.—to accomplish politico-strategic objectives; and 2) the unique insights it provides into making strategic moves aimed at influencing the choices taken by an adversary. However, strategic coercion theory as it currently stands is inadequate for applications against substate terrorist organizations. As a quintessential cornerstone for prescriptive policy in strategic studies, such a looming deficiency vis-à-vis one the most important security threats of the modern age is unacceptable. The new theoretical framework established in this dissertation—entitled the Balance Theory of strategic coercion—addresses this deficiency. The Balance Theory stresses that three key coercive elements of strategic coercion are fundamentally important for successfully ending the hostilities posed by NSTOs, being: A) Isolation of external/international support; B) Denial; and C) Isolation of popular support. It posits that these three aspects of strategic coercion serve as the sine qua non for success in countering an NSTO’s campaign of violence and effectively manipulating its decision-making process. Implementation of these three elements, moreover, must be pursued in tandem, taking care so as not to sacrifice one aspect for the other. The Balance Theory is tested through the employment of case-study analysis. In pursuing this end, both cross-case and within-case analyses are performed, accompanied by the utilization of the methods of focused, structured comparison. The cases examined are those of: 1) The United Kingdom versus Republican NSTOs (1969-2007); and 2) Israel versus Palestinian NSTOs (1967-present). The dissertation concludes with an examination of how the Balance Theory may provide insights for the formulation of counter-terrorism strategy against Al Qaeda in the current "War on Terror".