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Call number: LD2668 .T4 1962 F54

Call number: LD2668 .T4 1951 L46 / Master of Science

Electrophoresis is a widely used analytical technique in the medical and biotechnology industries. It can provide for the determination of thousands of individual compounds on this small scale. The operating conditions are quite conducive to use with the delicate products of genetic engineering. Due to other complexities associated with scale-up, the process is not widely used on a large scale. A novel recycling electrophoretic instrument was investigated as a preparative protein separation device. The process occurs in a thin film of liquid between two flat plates, in a direction perpendicular to the flow. This device was unique in its use of a relatively high flowrate, and recycling of the process fluid as a method for increasing residence time. The apparatus was operated in three modes, isoelectric focusing (IEF), zone electrophoresis (ZE) and isotachophoresis (ITP). For use in the ITP mode, a computer was used for data acquisition and control functions. Model systems included monoclonal antibodies and lentil lectins.

Published Article / A concept CPD framework, to be implemented by medical technology workers, was compiled and needed to be evaluated by professionals. The aim of this survey was therefore to use the Delphi technique as a research tool to evaluate the concept CPD framework by a widely distributed group of medical technology workers. The Delphi technique is a research tool organizing group communication in gaining consensus among a panel of experts. A monitoring team co-ordinated and evaluated the Delphi process in response to the feedback received by a panel of experts. Panellists are selected for their contribution to the topic under investigation. They do not normally interact with one another during the Delphi rounds. Fifteen panellists, distributed throughout South Africa, participated. The panellists were knowledgeable about medical technology and CPD. During the three Delphi rounds, statements were added, moderated, rephrased and rated. This resulted in a final CPD framework that consisted of 71 statements, completed within the three months scheduled time frame with a very high percentage co-operation of the panellists. The Delphi technique was an ideal tool for evaluating the concept CPD framework among a widely distributed group of medical technology workers.

published_or_final_version / abstract / Botany / Doctoral / Doctor of Philosophy

published_or_final_version / Pathology / Master / Master of Medical Sciences

The rate of oxidation of copper metal to copper (I) by copper (II) in the presence of organic ligands has been determined for a variety of ligands using a flow injection analysis system to sample the reaction. The results obtained using the FIA sampling method compare favorably with those obtained in eariler work when allyl alcohol was used as the ligand. The mechanism proposed in that earlier work has been shown to be a general mechanism for the oxidation of copper metal in the presence of complexing ligands. It was found that some ligands increase the rate of the reaction beyond the ability of conventional FIA to sample many data points before the reaction reaches equilibrium. A method has been developed that allows for more rapid sampling of the reaction mixture by the FIA system, resulting in overlapping FIA peaks. These overlapping peaks can then be deconvolved by an iterative curve fitting/digital subtraction technique in which each peak in the FIA output is fitted to a model function and subtracted from the output, thus removing its effect on the peak height of subsequent peaks. The iodination of acetylacetone in acidic solution was also studied by the fast injection FIA technique. Iodine was generated coulometrically in the solution containing acetylacetone and hydrochloric acid. By this method, the keto-enol equilibrium constant of acetylacetone was determined over a range of pH's. They ranged from 2.3 x 10⁻⁵ at a pH of 2.63 to 1.8 x 10⁻⁴ at a pH of 4.03. It was also found that the reaction was first order with respect to the acetylacetone concentration.