The focus of this project is the exploration of notions of the sacred through the study of functional, unadorned forms and the processes by which I translate them into drawn images. My objective is to observe and represent what I see as visually essential to objects such as bowl, egg and rag. My intention is to engage aspects of archetypal simplicity, providing a visual starting-point for contemplation in the viewer. Detached from specific liturgical contexts, the notion of 'sacredness' is not anchored by traditional icons. The logic behind the austerity of my 'generic' subject matter is to make a visual connection to the otherwise formless concept of a primal imprint of human consciousness, to anchor my exploration of the sacred. I aim to discover a position between figuration and abstraction that can communicate what I see as elemental to the subject of each drawing, concentrating the arena for contemplation within the bounds of the form depicted. By subduing contextual narrative in this way, I intend to amplify the tiny events of light, line and texture within the drawn form.

The implication of belief, that association between the veridical ghost tale and the fictional ghost tale—an association resulting from the onslaught of reason and science, and consequently spiritual doubt—remains largely responsible for the fictional ghost tale’s critical demise. A rise in the spiritualist movement produces a specific literature that coincides with the rise in interest in its fictional counterpart. Both the veridical ghost tale and the fictional ghost tale reach their heights in popularity at precisely the same time; not coincidental, but well planned by talented writers who viewed the preoccupation with ghosts as a platform from which a variety of contemporary issues could be candidly dealt. The Victorian literary ghost figure simultaneously, and ingeniously, fills a spiritual void, satisfies a consumer need for entertainment, and provides an opportunity for cultural commentary. The voice of the Victorian ghost, and the subsequent understanding of its haunted are of distinct cultural significance. / October 2004

För att installera flera operativsystem samtidigt finns det olika verktyg avsedda för att underlätta denna process. Det finns både kommersiella- och gratisalternativ ute på marknaden. En av de ledande kommersiella replikeringsmjukvarorna är Symantec Ghost. I detta arbete jämförs Symantec Ghosts mot Free open-source ghost (FOG) för att mäta användbarheten med avseende på kritiska attribut. Kritiska attribut är baserade på utförandet av en fullständig replikeringsprocess samt hur en användare upplever hanteringen av mjukvaran. Resultaten består av både kvalitativa och kvantitativa resultat som sammanställs i en jämförelsematris som presenterar för- och nackdelar med mjukvarorna. Resultaten visar att användbarheten är relativt likvärdig men att FOG presterar lite bättre än Symantec Ghost.

Thesis (Ph. D.)--University of Chicago, Divinity School, June 2001. / Includes bibliographical references. Also available on the Internet.

The processing of sensory signals is an important, yet complex task in which a system must extract behaviorally relevant stimulus patterns from a vast array of sensory cues. When a neuron within a major sensory area is presented with a stimulus, one of the important characteristics used to distinguish between types of input is frequency. Often sensory neurons are tuned to narrow stimulus frequency ranges and are thus charged with the processing of subtypes of sensory signals. The weakly electric fish Apteronotus lepthorhynchus senses it's environment through modulations of a self-generated electric field. Two main types of sensory signals can be distinguished based on their frequency patterns. Prey stimuli cause low frequency perturbations of the electric field, while communication signals often result in high frequency signals. Pyramidal neurons in the electrosensory lateral line lobe (ELL) encode the low frequency signals with bursts, while the high frequency signals are relayed with single spikes. This thesis describes how a pyramidal neuron's response patterns can be tuned to specific frequencies by the expression of distinct classes of potassium channels. / I have cloned 3 small conductance (SK) calcium activated potassium channels from cDNA libraries created from the brain of Apteronotus. I have subsequently localized the AptSK channels throughout the brain using both in situ hybridization (AptSK1, 2 & 3) and immunohistochemical (AptSK1 & 2) techniques. The 3 channels showed distinct expression patterns, with the AptSK1 & 2 channels showing a partially overlapping expression pattern, while AptSK3 appears to be expressed in unique areas of the brain. In the ELL AptSK1 & 2 show a partially overlapping expression pattern, appearing in similar pyramidal neurons. However, their distribution within individual cell is unique, with AptSK1 showing a dendritic localization, while AptSK2 is primarily somatic. We have demonstrated that the unique expression pattern of the somatic AptSK2 channel in the ELL coincides with the functional SK currents evaluated through in vitro electrophysiology. Further we have shown that neurons that encode low frequencies do not possess functional SK channels. It thus appears that the presence of the AptSK2 channel subtype can predispose a neuron to respond to specific types of sensory signals. / In an attempt to evaluate if second messengers could modify the AptSK control of frequency tuning I investigated the consequences of muscarinic acetylcholine receptor (mAChR) activation on a pyramidal neurons response patterns. While it had been shown in vivo that mAChR activation increased a pyramidal neuron's response to low frequencies, I have found that this was not due to a decrease in AptSK current, but rather appears to be the result of a down-regulation of an A-type potassium channel. / Taken together the studies that comprise this thesis show how the selective expression of a single potassium channel subtype can control a sensory neurons response to specific environmental cues. The secondary modulation of the A-type current highlights the potential for a second messenger to control a neuron's sensory response through the down-regulation of constitutively expressed potassium current.

Members of voltage-gated potassium channel subfamily 3 (Kv3) have been extensively demonstrated to play a significant role in facilitating function of "fast-firing" neurons in the central nervous system. Kv3.1 and Kv3.3 channels, members of Kv3 channel subfamily, have different distribution profiles on the regional level of brain and on the subcellular level of neurons in mammals and in weakly electric fish, according to mRNA hybridizations in situ and immunohistochemical analysis. In mammals, Kv3.1 channels are expressed in soma, axon and proximal dendrites as well as presynaptic membrane of "fast-firing" neurons. In weakly electric fish (Apteronotus), Kv3.1 channels are distributed in the soma, in the basilar dendrites and in the proximal apical dendrites of pyramidal neurons; on the other hand, Kv3.3 channels are expressed in a larger region: soma, basilar dendrites and entire apical dendrites of these cells. Mechanisms underlying differential subcellular distribution of Kv3.1 and Kv3.3 channels in the apical dendritic compartment of pyramidal neurons are unknown. In order to identify peptide sequences responsible for the differential subcellular localization, I have used Semliki Forest virus as a modified viral expression system (PDE) in vivo to study dendritic targeting mechanisms in the pyramidal neurons of electrosensory lateral line lobe (ELL), where the primary processing for afferent input occurs in the apteronotid electrosensory system.

This thesis explores the use of architecture in the ghost story "Episode of Cathedral History" by Montague Rhodes James. The focus entails an examination of the architectural theories of John Ruskin, which impacted James's personal views on education, female empowerment, and Modernism. These views are reflected in "Episode of Cathedral History" as story elements that bear symbolic values that James hides under the auspices of entertainment for the purpose of creating a commentary and warning about the chaos of the emerging Modern world.

The implication of belief, that association between the veridical ghost tale and the fictional ghost tale—an association resulting from the onslaught of reason and science, and consequently spiritual doubt—remains largely responsible for the fictional ghost tale’s critical demise. A rise in the spiritualist movement produces a specific literature that coincides with the rise in interest in its fictional counterpart. Both the veridical ghost tale and the fictional ghost tale reach their heights in popularity at precisely the same time; not coincidental, but well planned by talented writers who viewed the preoccupation with ghosts as a platform from which a variety of contemporary issues could be candidly dealt. The Victorian literary ghost figure simultaneously, and ingeniously, fills a spiritual void, satisfies a consumer need for entertainment, and provides an opportunity for cultural commentary. The voice of the Victorian ghost, and the subsequent understanding of its haunted are of distinct cultural significance.

Partial cDNAs for each of the four known N-methyl-D-aspartate (NMDA) receptor 2 (NMDAR2A-D) subunits have been cloned from the brain of A. leptorhynchus and are found to display a high degree of sequence homology (83--78% amino acid identity) to their mammalian homologues. In situ hybridization experiments reveal that each transcript has a distinct expression pattern in the apteronotid central nervous system (CNS) and is present in a "mosaic" distribution within important cell types of the electrosensory lateral line lobe (ELL). Apt. NMDAR2A transcript is expressed in forebrain regions as well as throughout the pyramidal cell layer (PCL) and granule cell layer (GCL) of the ELL. Apt. NMDAR2B mRNA is enriched in mid- and forebrain structures as well as the PCL and GCL of the ELL. Apt. NMDAR2C transcript is largely restricted to cerebellar regions but is also found in the PCL and GCL of the ELL's medial, centromedial, and centrolateral segments. Apt. NMDAR2D mRNA is expressed in sites of cell proliferation and in a segmental gradient within granule cells of the ELL.

Members of the Dlg/PSD-95 protein family have a modular organization with multiple protein interaction domains and are thought to be important in the organization of synapses. A degenerate primer PCR strategy was used to screen for members of this family in the central nervous system of Apteronotus leptorhynchus, which possesses an electrosensory system that is well-suited for the study of synaptic organization and the subcellular localization of proteins. A. leptorhynchus was found to express at least four Dlg/PSD-95 family genes as in mammals, and the full coding sequences of the homologues of mammalian PSD-95 and SAP102 were determined. In situ hybridization experiments performed for the A. leptorhynchus Dig/PSD-95 family members in brain showed that they have a differential pattern of expression.