Submitted by Alice Araujo (alice.caraujo@ufpe.br) on 2018-05-03T22:53:59Z No. of bitstreams: 1 DISSERTAÇÃO Patricia Duarte Costa Silva.pdf: 3456999 bytes, checksum: 56be3feb38164b0d9539609239d30a57 (MD5) / Made available in DSpace on 2018-05-03T22:53:59Z (GMT). No. of bitstreams: 1 DISSERTAÇÃO Patricia Duarte Costa Silva.pdf: 3456999 bytes, checksum: 56be3feb38164b0d9539609239d30a57 (MD5) Previous issue date: 2016-11-30 / CAPES / Thuja occidentalis Linn. (Cupressaceae), é tradicionalmente utilizada na homeopatia e fitoterapia para o tratamento de diversas doenças. A espécie vegetal é descrita por apresentar atividade antiviral, anti-inflamatória e anti-cancerígena, por isso, tem sido muito explorada para tratar condilomas provocados pelo Papilomavírus humano (HPV). Este é responsável por grande parte dos casos de câncer cervical em todo o mundo. Diversos tratamentos já estão disponíveis, contudo o arsenal terapêutico ainda é limitado, havendo a necessidade por novas alternativas de curas com baixos índices de efeitos adversos. Apesar dos relatos sobre atividade anti-HPV demonstrada por T. occidentalis, estudos direcionados à produção tecnológica de formas farmacêuticas preparadas a partir do vegetal ainda são escassos. Logo, o presente trabalho objetivou desenvolver e validar o método analítico por Cromatografia Líquida de Alta Eficiência (CLAE) para a quantificação do marcador quercitrina na tintura, no extrato bruto e na formulação semissólida contendo 10% da tintura de T. occidentalis. Adicionalmente, foram realizados testes preliminares de estabilidade da forma farmacêutica a fim de verificar possíveis indícios de instabilidade. A tintura a 10% (p/v) foi elaborada com etanol 70 °GL por digestão, sendo analisada quanto à densidade, teor alcóolico, resíduo seco e pH. O extrato bruto foi obtido pela secagem da tintura em evaporador rotativo, sendo avaliado também quanto ao teor de quercitrina por CLAE. Para o método de doseamento da tintura foi empregada coluna C18 (250 x 46 mm, 5 μm), a 32°C, fluxo de 0,8 mL/min com detecção de 255 e 345 nm. A fase móvel foi composta por água acidificada 0,3% de ácido acético pH 3,1 (A) e metanol (B) em sistema gradiente. O extrato bruto foi doseado em condições de análise semelhante aos da tintura com temperatura de 31°C. Os dois métodos foram validados segundo as normas oficiais e demonstraram resoluções adequadas para o marcador que eluiu aproximadamente em 17,45 min. Os métodos mostraram-se seletivos, específicos e lineares na faixa de 5-40 μg/mL e 5-50 μg/mL para a quercitrina na tintura e extrato bruto respectivamente. Exibiram boa precisão e exatidão, com recuperação acima de 99,84% do marcador, sem interferência da matriz vegetal. Os métodos apresentaram-se robustos com exceção para o tempo de retenção com a variação da temperatura. A tintura apresentou teor de quercitrina de 0,836 mg/mL e o extrato bruto, teor de 26,62 mg/g. Para a produção do gel foi utilizado os polímeros Carbopol 940® e hidroxipropilmetilcelulose (HPMC) com a finalidade de melhorar as propriedades mucoadesivas da formulação. No estudo de controle de qualidade, a forma farmacêutica apresentou pH compatível a mucosa vaginal com mucoadesão, espalhabilidade e viscosidade adequada. Na avaliação da estabilidade preliminar, a formulação fitoterápica apresentou conformidade, permanecendo estável após a realização da centrifugação, do estresse térmico e armazenamento à temperatura ambiente. O gel foi quantificado, fazendo pequenas modificações na metodologia previamente utilizada para a tintura nas mesmas condições de análise, a qual permitiu a recuperação de 19,17 mg/g de quercitrina a partir da matriz polimérica. Os métodos desenvolvidos mostraram ser adequados para o controle de qualidade da tintura e do extrato bruto, mas com necessidade de ajuste para o doseamento da formulação. / Thuja occidentalis Linn. (Cupressaceae), is traditionally used in homeopathy and herbal medicine for the treatment of various diseases. The plant species is described as having antiviral, anti-inflammatory and anticancer activity, so it has been extensively exploited to treat Human papillomavirus (HPV) condylomas. This is responsible for most of the cases of cervical cancer worldwide. Several treatments are already available, however the therapeutic arsenal is still limited, and there is a need for new cures alternatives with low rates of adverse effects. Despite the reports on anti-HPV activity demonstrated by T. occidentalis, studies aimed at the technological production of pharmaceutical forms prepared from the vegetable are still scarce. Therefore, the present work aimed to develop and validate the analytical method by High Performance Liquid Chromatography (HPLC) for quantification of the quercitrin marker in the tincture, in the crude extract and in the semisolid formulation containing 10% of the T. occidentalis tincture. In addition, preliminary tests of stability of the pharmaceutical form were carried out in order to verify possible signs of instability. The tincture at 10% (w/v) was elaborated with 70 °GL ethanol by digestion, being analyzed for density, alcohol content, dry residue and pH. The crude extract was obtained by drying the tincture in rotary evaporator, it was also evaluated the content of quercitrin by HPLC. For the tincture assay method was used C18 column (250 x 46 mm, 5μm) at 32°C, flow 0.8 mL/min with detection at 255 and 345 nm. The mobile phase was composed of acidified water 0.3% acetic acid pH 3.1 (A) and methanol (B) in the gradient system. The crude extract was dosed under similar analysis conditions as that of the tincture at a temperature of 31°C. Both methods were validated according to official standards and demonstrated adequate resolutions for the marker that eluted at approximately 17.45 min. The methods were selective, specific and linear in the range of 5-40 μg/mL and 5-50 μg/mL for quercitrin in tincture and crude extract, respectively. The methods showed good precision and accuracy, with recovery above 99.84% of quercitrin, without interference from herbal matrix. The methods were robust except for the retention time with temperature variation. Tincture had 0.836 mg/mL quercitrin content and the crude extract, 26.62 mg/g. For the production of the gel were used the Carbopol 940® and hydroxypropylmethylcellulose (HPMC) polymers to improve the mucoadhesive properties of the formulation. In the quality control study, the pharmaceutical form presented compatible pH to the vaginal mucosa with mucoadhesion, spreadability and adequate viscosity. In the evaluation of the preliminary stability, the phytotherapic formulation showed conformity, remaining stable after centrifugation, thermal stress and storage at ambient temperature. The gel was quantified, making small modifications in the methodology previously used for tincture under the same conditions of analysis, which allowed the recovery of 19.17 mg/g of quercitrin from the polymer matrix. The methods developed proved to be suitable for quality control of the tincture and crude extract, but in need of adjustment for the dosage of the formulation.

Plants and microorganisms interact with each other constantly, with some interactions being mutually beneficial and others being detrimental to the plants. The features of the organisms involved in such interactions will determine the characteristics of individual pathosystems. Plants respond readily to pathogen attacks, regardless of the pathosystem; furthermore, variation in the resistance to pathogens within species is common and well documented in many plant species. The variability in pathogen resistance is at the core of genetic improvement programs for disease resistance. True resistance to pathogens in plants is a genetically determined and complex trait that can involve both constitutive and induced mechanisms at different levels of organization. The complexity of this phenomenon makes the study of compatible plant - pathogen interactions challenging, and typically, disease resistance studies focus on specific aspects of a pathosystem, such as field resistance, anatomical or physiological features of resistant plants, or molecular mechanisms of resistance. The Thuja sp. - Didymascella thujina (E.J. Durand) Maire interaction is an important pathosystem in western North America, which has been studied for more than five decades. Western redcedar (Thuja plicata Donn ex D. Don) is very susceptible to cedar leaf blight (D. thujina), a biotroph that affects the tree at all stages, although seedlings are the most sensitive to the pathogen. The characteristics of the Thuja sp. - D. thujina interaction, the wealth of information on the pathosystem and the excellent Thuja sp. genetic resources available from the British Columbia Ministry of Forests, Lands, Natural Resource Operations and Rural Development make this interaction an ideal system to advance the study of disease resistance mechanisms in conifers. This Doctoral project presents a comprehensive investigation of the constitutive and induced resistance mechanisms against D. thujina in T. plicata, Thuja standishii (Gord.) Carrière and a Thuja standishii x plicata hybrid at the phenotypic and gene expression levels, undertaken with the objective of exploring the resistance mechanisms against the biotroph in these conifers. The project also aimed to establish base knowledge for the future development of markers for marker-assisted breeding of T. plicata. The investigations included a combination of histological, chemical and next generation sequencing (NGS) methodologies. NGS data were analyzed, in addition to the traditional clustering analyses, with cutting edge machine learning methods, including grade of membership analysis, dynamic topic modelling and stability selection analysis. The studies were progressively more controlled to narrow the focus on the resistance mechanisms to D. thujina in Thuja sp. Histological characteristics related to D. thujina resistance in Thuja sp. were studied first, along with the relationship between climate of origin and disease resistance. The virulence of D. thujina was also documented early in this project. Chemical and gene expression constitutive and induced responses to D. thujina infection in T. plicata seedlings were studied next. T. plicata clonal lines were then comprehensively studied to shed light on the mechanisms behind known physiologically determined resistance. A holistic investigation of the resistance mechanisms to D. thujina in T. standishii, T. plicata and a T. standishii x plicata hybrid explored the possibility of a gene-for-gene resistance model. Thirty-five T. plicata families were screened during the four field seasons carried out between 2012 and 2015, totalling more than 1,400 seedlings scored for D. thujina severity. Thirteen of those families were used in the five studies performed during the program, along with two T. plicata seedling lines self-pollinated for five generations and three T. plicata clonal lines. One T. standishii clonal line, and one T. standishii x plicata clone were also investigated during the program. A total of 16 histological and anatomical characteristics were studied in more than 750 samples, and more than 270 foliar samples were analyzed for 60 chemical and nutritional compounds. Almost one million transcriptomic sequences in four individually assembled reference transcriptomes were examined during the program. The results of the project support the variability in the resistance to D. thujina in T. plicata, as well as the higher resistance to the pathogen in plants originating from cooler and wetter environments. The data collected also depicted the existence of age-related resistance in T. plicata, and confirmed the full resistance to the disease in T. standishii. Western redcedar plants resistant and susceptible to D. thujina showed constitutive differences at the phenotypic and gene expression levels. Resistant T. plicata seedlings had thicker cuticles, constitutively higher concentrations of sabinene, alpha-thujene, and higher levels of expression of NBS-LRR disease resistance proteins. Resistant clones of T. plicata and T. standishii had higher expression levels of bark storage proteins and of dirigent proteins. Plants from all ages, species and resistance classes studied that were infected with D. thujina showed the accumulation of aluminum in the foliage, and increased levels of sequences involved in cell wall reinforcement. Additional responses to D. thujina infection in T. plicata seedlings included the downregulation of some secondary metabolic pathways, whereas pathogenesis-related proteins were upregulated in clonal lines of T. plicata. The comprehensive approach used here to study the Thuja sp. - D. thujina pathosystem could be applied to other compatible plant-pathogen interactions. / Graduate / 2020-08-31

Ring-width chronologies from Pinus resinosa Ait., Pinus strobus L., and Thuja occidentalis L. were developed in two areas of the Boundary Waters Canoe Area Wilderness to assess their growth climate response and their potential for developing reconstructions of climate. New red pine chronologies were combined with existing chronologies to extend the ring-width record both into the past and into the present. Ring-width response to climate, assessed using correlation analysis and response functions, was broadly similar among all three species with relatively significant positive relationships with June–July precipitation and significant negative (but less consistent) associations with June–July temperatures (p < 0.05). White-cedar appeared to have a broader phenological window of response with a stronger spring influence when compared to other species included in this study. Comparisons with other nearby proxies showed relatively strong coherence overall but with some important regional differences. Overall, these species may be useful for placing current climatic patterns in the Boundary Waters within a longer term perspective but care should be taken with respect to identifying appropriate climatic records for calibration.

Western redcedar (Thuja plicata Donn ex D. Don; redcedar), an indeterminate conifer in the Cupressaceae family, is vulnerable to maladaptation in the face of climate change. Assisted gene flow is one mitigation strategy and involves human-mediated migration of populations, where the projected climate of the area of deployment matches the source climate of the population. Despite the overall projections of warmer temperatures globally, in British Columbia (B.C.), the risk of seasonal frost events will remain and therefore the potential for cold damage and mortality of redcedar exists if the newly migrated populations cannot withstand these freezing events. Knowledge of redcedar's ability to withstand freezing temperatures (cold hardiness) is therefore crucial. Redcedar, like many Cupressaceae species, produces and accumulates the purple-coloured carotenoid rhodoxanthin during the winter. This was hypothesized to be correlated with cold hardiness. Assessment of variation in overall, fall and spring cold hardiness and associated rhodoxanthin concentrations were done through repeated, seasonal freeze testing of clonal grafts originating from across the range of redcedar, and seedling progeny from a subset of these clones. Cold damage was quantified using electrolyte leakage and rhodoxanthin concentrations were quantified using high performance liquid chromatography. Cold hardiness and rhodoxanthin were individually modelled using univariate and bivariate mixed effect models with clone/family as a random effect. Model outputs were compared to climatic variables associated with clonal origin to test for climatic relationships. This study found genetic variation in cold hardiness of redcedar with weak climatic clines. This indicates that assisted gene flow of redcedar should be done on a case-by-case basis, with no need for a climatic threshold. Overall heritability of cold hardiness was 0.17 ± 0.03. Novel findings included the positive genetic correlation between fall and spring cold hardiness (0.55 ± 0.33); lack of reciprocal or parental effect for overall cold hardiness; and weak climatic relationships between cold hardiness and predominantly temperature, with the strongest correlation between number of frost-free days in January (0.38, p < 0.01) in the location of origin and cold hardiness. All findings related to rhodoxanthin were novel. Rhodoxanthin varied with family/provenance and season with heritabilities of 0.30 ± 0.09 in fall, 0.42 ± 0.09 in winter and 0.28 ± 0.09 in spring. Winter and spring rhodoxanthin concentrations were phenotypically correlated (0.50, p < 0.01) and genetically correlated (0.76 ± 0.14). Surprisingly, rhodoxanthin was not detected in clonal grafts of redcedar in any season. Results also indicate that rhodoxanthin cannot be used to estimate cold hardiness. The absence of rhodoxanthin in the clonal grafts compared to the seedlings suggests that plant age impacts rhodoxanthin accumulation. / Graduate / 2021-12-14

Cedar, represented by Western redcedar (Thuja plicata) and Yellow Cedar (Chamaecyparis nootkatensis) was known as the “Tree of Life” to the Nuu-chah-nulth on Vancouver Island’s west coast, and most other groups of the Pacific Northwest. This thesis investigates the Culturally Modified Trees (CMTs), or more specifically Tapered Bark Strips (TBS), created through the extraction of cedar bark removed for all manner of material goods. CMTs are now the most common archaeological site type within British Columbia. Current regional chronologies have inherent biases that make interpretations difficult. The chronologies created through Archaeological Impact Assessments (AIAs) are weighted heavily to the contact period and the highest frequency of use corresponds with indigenous population collapse rather than peak. Investigations are made into the true distribution of existing CMT features. This thesis details the survey of 16 recent old growth cedar clearcuts which found extensive unrecorded CMT features that have recently been logged throughout the southern Nuu-chah-nulth study region. Half of all TBS scars in exposed stumps were found embedded within healed trees, otherwise invisible to archaeologists. Comparing all AIA report dates (surveyed prior to logging activity) with all post-impact assessments surveys it was found the latter contain a greater and older distribution of scarring events corresponding to high First Nations populations before the contact period. The study also compares CMT chronologies with local histories, investigates the antiquity of Northwest Coast CMTs and the indigenous management of cedar trees to maximize bark harvests. The findings of this research hint at the expanded extent of anthropogenic forests in the Northwest Coast, the inadequate recording and heritage protections of CMTs, and what it all means for Aboriginal Land Rights in British Columbia. / Graduate / 0324 0740 0329 / kinze.earnshaw@gmail.com

La loupe de thuya joue un rôle économique et social important pour le secteur artisanal marocain. Ces dernières décennies, pour répondre à une forte demande, le bois de la loupe de thuya a été surexploité entraînant une dégradation importante des forêts de thuya marocaines. Une réponse possible à cette problématique, à court terme, consiste à limiter les déchets d'usinage. Cette voie d'investigation passe par la caractérisation du matériau et notamment l'identification de son réseau de symétries matérielles. L'objectif de ce travail est donc de caractériser le bois de la loupe de thuya (Tetraclinis articulata (vahl) Masters) dont le comportement mécanique n'a pas été étudié jusqu'à présent. Pour atteindre cet objectif, on passe tout d'abord par une caractérisation multi-échelle. A l'échelle globale, la caractérisation est réalisée en étudiant la structure interne d'une loupe entière par tomographie X, et plus particulièrement la distribution des excroissances foncées caractéristiques de la loupe. Cette étude macroscopique est complétée, à l'échelle microscopique, par une analyse comparative de coupes anatomiques de bois de thuya et de loupe. À l'échelle du matériau, le bois de la loupe est caractérisé en étudiant la dispersion de sa rigidité par une méthode ultrasonore par contact direct. L'étude plus approfondie du comportement mécanique a nécessité la mise au point d'une méthode ultrasonore par contact direct sur des échantillons sphériques afin d'identifier les symétries matérielles d'un matériau. Cette méthode, appliquée au bois de la loupe de thuya, montre que la loupe a un comportement isotrope transverse. Par conséquent, il ne présente pas les mêmes symétries naturelles que le bois présent dans le tronc du même arbre. Cette méthode ne permet pas d'obtenir la matrice de rigidité complète : dans le cas d'un matériau orthotrope, elle donne accès aux 3 termes diagonaux de la matrice et à 3 combinaisons linéaires des 6 autres termes. Pour identifier complètement la matrice de rigidité, nous proposons, en perspective, l'utilisation de la Spectroscopie Ultrasonore Résonante (RUS). Le couplage de la méthode ultrasonore par contact direct développée et de la méthode RUS permettra de déterminer les symétries matérielles et la matrice de rigidité sur le même échantillon. Enfin, des coupes obtenues par tomographie X peuvent être superposées aux graphes de rigidité pour analyser la relation entre la structure interne et le comportement élastique de la loupe de thuya. / The wood of thuja burr plays an important economic and social role for the Moroccan artisanal sector. In last decades, as demand increased, thuja burr was overexploited resulting in an important degradation of Moroccan thuja forests. One possible solution, in the short term, consists in limiting waste machining. This approach requires the characterization of the material, especially of its materiel symmetries. This work aims thus at characterizing the wood of thuja burr (Tetraclinis articulata (Vahl) Masters), that was little studied up to now. First, a multi-scale characterization is proposed. At the global scale, the internal structure of an entire thuja is observed using X ray tomography, especially the distribution of dark growth elements typical of thuja burr. This macroscopic characterization is completed, at the microscopic scale, by a comparative analysis of anatomical microsections of thuja wood and thuja burr. At the material scale, the dispersion of the thuja burr stiffness is observed using a direct contact ultrasonic method. In order to progress further in the study of the mechanical anisotropy, a direct contact ultrasonic method for spherical samples is developed allowing to identify material symmetries. By applying this method to the thuja burr wood, a transverse isotropic behaviour is observed. Hence thuja burr wood does not exhibit the same natural symmetries as what is found in the trunk of the same tree. However, this method does not yield the complete stiffness matrix. In the case of an orthotropic material, it gives the access to 3 diagonal terms of the matrix and 3 linear combinations of the six other terms. The use of Resonance Ultrasound Spectroscopy (RUS) is proposed to identify the stiffness matrix completely: the coupling of the direct contact ultrasonic method developed and RUS method allows to determine the symmetries and stiffness matrix on the same sample. Finally, scanned images obtained by X-ray tomography can be superimposed on stiffness graphs to analyze the relationship between the internal structure and the elastic behavior of thuja burr.

Ce mémoire porte sur le cèdre blanc (Thuja occidentalis) dans les contextes de colonisation et d’exploitation forestière de l’arrière-pays montréalais au XIXe siècle. Il vise, d’une part, à documenter les stratégies d’exploitation locale du cèdre blanc au XIXe siècle et l’évolution du paysage culturel domestique d’un établissement colonial depuis sa concession initiale. D’autre part, ce mémoire cherche à identifier les réseaux d’échanges du cèdre blanc acheminé à Montréal au XIXe siècle pour la construction des bâtiments et des infrastructures portuaires. En raison de la quasi-absence de documents historiques sur le sujet, il devient évident que seule l’application de la dendrochronologie et de la dendroprovenance permet d’atteindre ces objectifs. Nous vous présentons ici l’analyse détaillée de six sites ruraux situés dans les vallées de l’Outaouais et du haut Saint-Laurent. Les analyses dendrochronologiques effectuées permettent d’aborder l’établissement colonial d’une façon originale. La deuxième partie de ce mémoire se consacre aux analyses de dendroprovenance de sept sites montréalais préalablement étudiés par Poudret-Barré (2007) et le Groupe de recherche en dendrochronologie historique (GRDH). Pour ce faire, les sites ruraux discutés précédemment servent de point d’ancrage géographique afin de déterminer l’origine des pièces de cèdre blanc retrouvées à Montréal. L’étude du cèdre blanc et les résultats des analyses de dendrochronologie et de dendroprovenance réalisées dans le cadre de cette étude permettent d’aborder le patrimoine architectural et archéologique sous un angle nouveau. Venant compléter les données historiques disponibles, il ouvre la voie à de nouvelles recherches de ce genre. / This master’s thesis is about the colonization context and forestry exploitation of eastern white cedar (Thuya occidentalis) in the Montreal hinterland of the 19th century. On the one hand, it aims to document the local exploitation strategies of eastern white cedar and the evolution of the domestic cultural landscape of colonial landholdings since their initial concession. On the other hand, this thesis seeks to identify the exchange networks of the cedar timber that was shipped to Montreal for the construction of the city’s buildings and port facilities. With the near absence of historical documentation on the subject, only the application of the dendrochronology and the dendroprovenancing allows us to attain our objectives. We present a detailed analysis of six rural sites in the Ottawa and the Upper Saint Lawrence Valleys. Dendrochronology analysis of these areas enables us to tackle colonial establishment in an original way. The second part of this thesis is dedicated to the dendroprovenancing analysis of seven Montreal sites, all studied beforehand by Poudret-Barré (2007) and the Groupe de recherches en dendrochronologie historique (GRDH). To do so, the previously designated sites will serve as a geographic anchor point to help us determine the origin of the pieces of eastern white cedar found in Montreal. Our study of eastern white cedar along with the results of the dendrochronology and dendroprovenancing analysis allows us to tackle the architectural and archeological heritage from a new angle. Completing the available history data, it also opens the way to new research of the same kind.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Ce mémoire porte sur le cèdre blanc (Thuja occidentalis) dans les contextes de colonisation et d’exploitation forestière de l’arrière-pays montréalais au XIXe siècle. Il vise, d’une part, à documenter les stratégies d’exploitation locale du cèdre blanc au XIXe siècle et l’évolution du paysage culturel domestique d’un établissement colonial depuis sa concession initiale. D’autre part, ce mémoire cherche à identifier les réseaux d’échanges du cèdre blanc acheminé à Montréal au XIXe siècle pour la construction des bâtiments et des infrastructures portuaires. En raison de la quasi-absence de documents historiques sur le sujet, il devient évident que seule l’application de la dendrochronologie et de la dendroprovenance permet d’atteindre ces objectifs. Nous vous présentons ici l’analyse détaillée de six sites ruraux situés dans les vallées de l’Outaouais et du haut Saint-Laurent. Les analyses dendrochronologiques effectuées permettent d’aborder l’établissement colonial d’une façon originale. La deuxième partie de ce mémoire se consacre aux analyses de dendroprovenance de sept sites montréalais préalablement étudiés par Poudret-Barré (2007) et le Groupe de recherche en dendrochronologie historique (GRDH). Pour ce faire, les sites ruraux discutés précédemment servent de point d’ancrage géographique afin de déterminer l’origine des pièces de cèdre blanc retrouvées à Montréal. L’étude du cèdre blanc et les résultats des analyses de dendrochronologie et de dendroprovenance réalisées dans le cadre de cette étude permettent d’aborder le patrimoine architectural et archéologique sous un angle nouveau. Venant compléter les données historiques disponibles, il ouvre la voie à de nouvelles recherches de ce genre. / This master’s thesis is about the colonization context and forestry exploitation of eastern white cedar (Thuya occidentalis) in the Montreal hinterland of the 19th century. On the one hand, it aims to document the local exploitation strategies of eastern white cedar and the evolution of the domestic cultural landscape of colonial landholdings since their initial concession. On the other hand, this thesis seeks to identify the exchange networks of the cedar timber that was shipped to Montreal for the construction of the city’s buildings and port facilities. With the near absence of historical documentation on the subject, only the application of the dendrochronology and the dendroprovenancing allows us to attain our objectives. We present a detailed analysis of six rural sites in the Ottawa and the Upper Saint Lawrence Valleys. Dendrochronology analysis of these areas enables us to tackle colonial establishment in an original way. The second part of this thesis is dedicated to the dendroprovenancing analysis of seven Montreal sites, all studied beforehand by Poudret-Barré (2007) and the Groupe de recherches en dendrochronologie historique (GRDH). To do so, the previously designated sites will serve as a geographic anchor point to help us determine the origin of the pieces of eastern white cedar found in Montreal. Our study of eastern white cedar along with the results of the dendrochronology and dendroprovenancing analysis allows us to tackle the architectural and archeological heritage from a new angle. Completing the available history data, it also opens the way to new research of the same kind.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal