Quarenta e nove amostras EPEC típica (tEPEC) e atípica (aEPEC) pertencentes a diferentes sorotipos, isoladas de humanos e animais (cães, gatos, bovinos, ovinos, coelhos e sagüis) foram investigadas quanto ao perfil de virulência pela PCR e similaridade clonal por Multilocus Sequence Typing (MLST) e Pulsed-Field Gel Electrophoresis (PFGE). O objetivo deste estudo foi verificar se animais atuam como reservatório e fonte infecção de aEPEC para humanos. Os marcadores de virulência analisados revelaram que cepas aEPEC isoladas de animais possuem potencial para causar diarréia em humanos. As técnicas MLST e PFGE revelaram que amostras isoladas de animais e humanos compartilham relações clonais próximas ou idênticas. Estes resultados indicam que os animais estudados atuam como reservatório de aEPEC e representam fonte de infecção para humanos. Pelo fato de humanos, também atuarem como reservatório de aEPEC, ciclos de infecção cruzada animal-humano não podem ser descartados, pois a dinâmica de transmissão entre reservatórios de aEPEC não é muito bem compreendida. / Forty-nine typical and atypical EPEC strains belonging to different serotypes, isolated from humans, pets (cats and dogs), farm (bovines, sheep and rabbits) and wild animals (monkeys) were investigated for virulence markers and clonal similarity by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The virulence markers analyzed revealed that atypical EPEC strains isolated from animals have the potential to cause diarrhea in humans. Close clonal relationship between human and animal isolates was found with MLST and PFGE. These results indicate that these animals act as atypical EPEC reservoirs and may represent sources of infection for humans. Since humans also act as a reservoir of atypical EPEC strains, the cycle of mutual infection of atypical EPEC between animals and humans, mainly pets and their owners, cannot be ruled out, since the transmission dynamics between the reservoirs are not yet clearly understood.

A mediação do professor é fundamental para a construção de argumentos em aulas de Ciências. Nesse sentido torna-se importante estudar as interações discursivas entre professor e alunos. O presente trabalho teve por objetivo analisar como os propósitos e as ações do professor estimulam a argumentação dos alunos em uma aula investigativa de ciências. Para caracterizar a mediação do professor foi utilizada a ferramenta analítica \"propósitos epistêmicos e ações típicas para promoção da argumentação\" de Ferraz e Sasseron (2014) e de Ferraz (2015), enquanto que o layout de Toulmin (TAP) (2006) foi utilizado para identificar os elementos do argumento construídos pelos alunos. Foram identificados dois argumentos contendo os elementos básicos do TAP construídos coletivamente ao longo da aula. Durante a construção dos argumentos, o professor utilizou os propósitos retomar, problematizar, explorar e qualificar. As ações \"retoma dado\" e \"explora ponto de vista\" contribuíram para a construção do elemento dado. A construção da garantia deveu-se as ações \"retoma conceito\", \"retoma informações\", \"explora ponto de vista\" e \"qualifica variáveis, objetos ou fenômenos\". A construção do elemento conclusão foi promovida pela ação \"propõe um problema\". As ações \"qualifica variáveis, objetos ou fenômenos\", \"qualifica explicações ou pontos de vista\", \"retoma informações\" e \"explora ponto de vista\" auxiliaram na construção do elemento refutação do TAP. A maior frequência dos propósitos explorar e qualificar deveu-se ao esforço do professor em promover a avaliação de dados e garantias que os alunos estavam utilizando para a construção de suas conclusões sobre a presença ou não de seres vivos na amostra analisada. / The mediation of the teacher is fundamental for the construction of arguments in science class. Like this it is important to study the discursive interactions between teacher and students. This study aimed to analyze how the teacher\'s purposes and actions stimulate the reasoning of students in an inquiry science class. The analytical tool \"epistemic purpose and typical actions to promote the argumentation\" (FERRAZ; SASSERON, 2014; FERRAZ, 2015) was used to characterize the mediation of the teacher while the Toulmin Argument Pattern (TAP) (2006) was used to identify argument elements built by students. Two arguments containing the basic elements of TAP collectively built along class were identified. During the construction of the arguments, the teacher used the purpose resume, problematize, explore and qualify. The actions \"given recovery\" and \"explores view\" contributed to the construction of the data element. The construction of the warrants was due to the actions \"recovery concept\", \"recovery information\", \"explores points of view\" and \"qualifies variables, objects or phenomena\". The construction element conclusion was promoted by the action \"proposes the problem\". The actions \"qualifies variables, objects or phenomena\", \"qualifies explanations or points of view\", \"recovery information\" and \"explores perspective\" helped in the construction of refutation element of TAP. The highest frequency of the purposes explore and qualify was due to the teacher\'s effort to promote the evaluation of data and warrants that students were using to build its conclusions on the presence or absence of living organisms in the sample.

Este trabalho tem por objetivo propor uma nova metodologia para o cálculo das perdas técnicas de energia e demanda por segmento do sistema de distribuição. As perdas técnicas foram divididas em oito segmentos: medidor de energia, ramal de ligação, rede secundária, transformador de distribuição, rede primária, subestação de distribuição, sistema de alta tensão e outros. Neste último segmento, foram incorporadas as parcelas referentes a equipamentos (capacitores, reguladores de tensão, etc), perdas em conexões, corrente de fuga em isoladores, etc. Desenvolveu-se um sistema computacional, para uso em microcomputador, que conta com dois módulos principais. O primeiro módulo destina-se ao cálculo das perdas técnicas em redes específicas. O cálculo é feito de forma hierárquica, por exemplo, selecionada uma subestação específica, são calculadas as perdas na subestação e em todos os componentes a jusante (redes primárias, transformadores de distribuição, redes secundárias, ramais de ligação e medidores de energia). As perdas técnicas, em termos de energia e demanda, são obtidas por meio de cálculo elétrico específico para os segmentos envolvidos, com a utilização dos dados cadastrais da rede, dados de faturamento e curvas de carga típicas por classe de consumidor e tipo de atividade desenvolvida. Com a aplicação deste módulo para todo o sistema da empresa, ou para uma parcela representativa, obtém-se um índice percentual de perda de energia para cada segmento. Estes índices são transferidos para o segundo módulo, que tem por objetivo efetuar o balanço de energia do sistema. A partir dos dados de energia nos pontos de suprimento, da energia total faturada mensalmente e dos índices de perdas por segmento, são obtidos os montantes de energia de perdas em cada segmento do sistema e uma avaliação das perdas não técnicas. O trabalho ainda apresenta um exemplo de aplicação, em sistema de distribuição real, mostrando os resultados obtidos, e termina apresentando as principais vantagens da metodologia. Os resultados obtidos com a nova metodologia são comparados com os resultados obtidos com outras metodologias. / This work focuses on the development of a new methodology for calculating technical losses within the different segments of the electric distribution system. In order to evaluate technical losses the electrical system was divided into eight segments: energy meter, customer connections, secondary feeder, distribution transformer, primary feeder, distribution substation, high voltage system and another segment that represents other technical losses. The latter comprises losses in equipment such as capacitors and voltage regulators as well as losses in electric connections, isolators, etc. A computational system was developed and it comprises two modules. The first one is intended to calculate technical losses in specific networks. The calculation is made in a hierarchical manner. If a distribution substation is selected for the calculation, the losses in that substation and the losses in all components downstream are calculated. The technical losses, in terms of energy and demand, are obtained by utilizing network topological data, consumed energy data and typical load curves per customer class and activity type. An energy losses index, calculated as a percentage of the supplied energy, is obtained for each segment by applying this module to the entire utility’s power system or in a representative part of it. These values are transferred to the second module, which has the objective of determining the overall energy balance. Technical and non-technical losses, in terms of energy, in each segment are obtained by utilizing the total amount of energy supplied to the system, energy consumed and energy losses indices per segment. This work shows the results obtained by applying the computational tool to a real distribution system. Such results are compared with the ones obtained by using other methodologies, and the main advan ages of this new methodology are outlined.

Past tense verb production in children with specific language impairment and language-matched children with typical language was compared using language samples and a standardized probe (Rice/Wexler Test of Early Grammatical Impairment). Analyses revealed accuracy and error type differences between elicitation types and groups. Results have important clinical practice implications.

Regular (e.g., jumped) and irregular (e.g., fell) past tense verb acquisition in children with typical language development (TL) occurs between ages 3-5. In children with specific language impairment (SLI), acquisition of these forms is extended and errors in spontaneous conversation may even continue into adulthood. However, there is a lack of consensus as to whether probed or spontaneous language samples give a more accurate representation of a child’s linguistic skills. The first aim of this study was to determine if there were differences in regular and irregular past tense verb production accuracy between two Elicitation Methods: probed vs. spontaneous language sampling. The second aim was to determine if accuracy and error patterns differed between children with SLI and children with TL. The participants included 11 children with SLI (mean age: 5 years) and 20 children with TL (mean age: 3 years 6 months). Each participant received a battery of tests to determine language status. This battery included two elicitation methods for regular and irregular past tense: a probe and a spontaneous language sample. The Rice/Wexler Test of Early Grammatical Impairment probed past tense verb production using picture prompts and a standardized verbal routine. Additionally, a language sample was recorded in which participants told three thematically related stories provided spontaneous productions. The first two stories were read by the examiner first and the child was asked to retell it. The first story was presented in the present tense. The second story was presented in the past tense. The third story was made up by the child based on the pictures and the tense was free to vary. These stories provided the language sample that was then transcribed and coded for a statistical analysis of verb production. Within and between groups ANOVAs revealed statistically significant differences between the probe and spontaneous language samples, with the probe yielding higher accuracy for regular and irregular past tense verb production in both groups. There was no significant Group effect or Group by Elicitation Method interaction. Analysis of the types of errors produced revealed a statistically significant Group by Elicitation Method interaction. Post hoc analysis found for regular past tense verbs, children with SLI produced more stem-form errors than children with TL. For irregular past tense forms, children with SLI produced more stem form errors, while children with TL produce more overregularization errors. The observed pattern of errors is consistent with inclusionary criteria for SLI, the literature, and theoretical foundations. The results add to the literature about the accuracy of probe and spontaneous language sample elicitation methods.

Speech-language pathologists are challenged with providing children with treatment that results in the greatest amount of improvement in the least amount of time. To aid in this challenge, we examined how quickly and efficaciously seven children with a specific language impairment (SLI) learned verbs compared to seven children with typical language (TL) development. Each child was taught the meaning and use of six nonsense verbs. Nonsense verbs were used to ensure that the target verbs had never been heard by any of the participants before the experiment and allowed us to control verb exposure. Each participant received up to four training sessions of 30-minutes each. During these sessions, the examiner utilized naturalistic conversational techniques and play activities with carefully selected sets of toys that corresponded to the meaning of the target words. The training sessions were ended when children demonstrated 100% accuracy in their understanding of the verbs and 50% accuracy in their production during a probe task. All sessions were audio-recorded and the experimenters and childs utterances were reliably coded. Analysis revealed that the two groups received the same input from the experimenter in terms of the number and type of exposures to the verbs. We examined the childrens nonsense verb productions for their rate and quality. Rate of verb acquisition was measured by counting: 1) the number of sessions required before the criteria for learning was met; 2) the number of exposures before childs first spontaneous production of each target verb; and 3) the number of exposures before childs first elicited production of each target verb. Quality of verb acquisition was measured by counting: 1) the number of alternative real verbs substituted for a nonsense verb and 2) the accuracy of transitive target verb usage. Although the results were not statistically significant for any of the measures tested, there was a trend for children with SLI to learn the target nonsense verbs more slowly but just as

One in 59 children is identified as having an Autism spectrum disorder (ASD). The Individuals with Disabilities Education Act (IDEA) mandates that students with disabilities be educated in the general education setting with typical peers to the maximum extent possible. This practice of inclusion has led to increased social-isolation and peer rejection among students with ASD. Research suggests inclusion alone without implementing peer intervention training is ineffective in fostering positive interactions between students with ASD and their typical peers. Therefore, the purpose of this study is to contribute to the literature by evaluating a peer educational intervention designed to promote positive peer relations among students with ASD, as well as, examine the effects of a peer educational intervention on typical peers’ perceived level of self-efficacy in interacting with students with ASD.

Le récent développement des nanotechnologies induit des expositions potentielles aux nanoparticules dans une diversité de milieux professionnels. Une meilleure appréhension des risques et des expositions professionnelles aux nanoparticules représente alors un enjeu essentiel pour les acteurs de la prévention, ce qui nécessite d’enrichir, voire de rompre, avec les approches classiques de métrologie et de prévention. Dans ce contexte, notre thèse se centre sur le développement d’une méthodologie transdisciplinaire intégrant l’analyse de l’activité à la mesure afin de produire des connaissances sur l’exposition aux nanoparticules et sa maîtrise. A partir de la construction et de la validation d’une méthodologie par des spécialistes de la mesure des nanoparticules, de l’épidémiologie et de l’ergonomie, nos résultats s’orientent autour de trois axes. 1) La revue systématique de la littérature des préconisations actuelles pour évaluer les expositions professionnelles aux nanoparticules met en avant l’existence de 23 documents centrés sur la mesure des nanoparticules manufacturées seulement sans s’intéresser aux nanoparticules émises non intentionnellement par certains procédés industriels. Par ailleurs, ces recommandations à destination des préventeurs doivent évoluer pour une meilleure prise en compte de l’activité de travail et devenir opérationnelles. 2) La méthode d’évaluation de l’exposition produite tenant compte de ces limites, est passée par une mobilisation des acteurs de l’entreprise pour collecter des informations sur les situations potentielles d’exposition. Ensuite, des mesurages en temps réel et intégrés des aérosols, accompagnés d’enregistrements de la fréquence cardiaque et respiratoire sont synchronisés à des vidéos de situations de travail. Un découpage à différentes échelles temporelles en phase de l’activité ou en action détaillée de l’opérateur, conduit à révéler des situations d’exposition réelles aux nanoparticules, et évaluer leurs niveaux d’exposition intégrant l’intensité physique. 3) Nous montrons que les échanges avec les opérateurs lors d’entretiens de confrontation aux vidéos et mesures synchronisées, permettent de rendre visibles ces situations d’exposition et leurs déterminants, sous forme de situations d’exposition caractéristiques. Cette présentation des résultats de l’analyse de l’activité, contribue à comprendre et mettre en perspective les pratiques de sécurité formelles pour les faire évoluer. C’est par ces échanges collectifs que les déterminants de l’exposition sont découverts, ce qui permet aux différents acteurs de renforcer la maîtrise de l’exposition. L’usage des situations d’exposition est aussi un moyen d’agir sur les projets de conception en transférant des repères pour la conception et la prévention. Il devient alors possible d’enrichir les évaluations de l’exposition aux nanoparticules et contribuer à la construction collective de la sécurité dans l’innovation. / The recent development of nanotechnologies has led to potential exposures to nanoparticles in a variety of workplaces. A better understanding of occupational exposures to nanoparticles represents a major stake for prevention stakeholders. These exposures require an overhaul of, and perhaps a break from traditional metrology and prevention approaches, as they are challenged by the particular characteristics of nanoparticles. In this context, this thesis focuses on the development of a transdisciplinary methodology integrating work activity analysis with measurement in order to produce knowledges on exposure to nanoparticles and its control.From the construction and the validation of a methodology between specialists in nanoparticle measurement, epidemiology and ergonomics, our results follow three axes. 1) The systematic review of the literature on current recommendations for assessing occupational exposures to nanoparticles highlights the existence of 23 documents focusing on the measurement of manufactured nanoparticles only, excluding nanoparticles emitted unintentionally by some industrial processes. In addition, these recommendations for preventionists must evolve to take better account of work activity and become operational. 2) The exposure assessment method developed in this thesis work is based on the mobilization of the company's stakeholders to gather information on potential exposure situations. Then, real-time as well as time averaged aerosol measurements, accompanied by heart rate recording, were synchronized with videos of work situations. A breakdown at different time scales, at the work activity stage or in more detailed action carried out by the operator, revealed real exposure situations to nanoparticles, and characterized the associated exposure levels, taking into account the physical intensity of the work. 3) We show that discussions with operators during confrontation interviews involving real-time measurement synchronized to videos of the work activity, made it possible to make these exposure situations and their determinants visible, in the form of “typical exposure situations”. Presenting the results in association with the work activity analysis in the companies contributes to understanding and questioning the regulated safety practices to make them change. It is through these collective exchanges that the determinants of exposure are discovered, allowing to the company’s stakeholders to improve exposure control. The use of exposure situations is also a means of influencing design projects by transferring requirements for conception or prevention. It therefore becomes possible to expand exposure assessment to nanoparticles and contribute to collectively build safety within innovation.

Tesis para optar al Grado de Magíster en Dirección y Administración de Proyectos Inmobiliarios / Se trata de intereses ejercidos hacia el mismo espacio urbano. Tanto patrimonio como mercado inmobiliario corresponden a áreas de estudio confrontadas, situación bastante lógica si piensa en ciertos grupos patrimoniales que preservan sus construcciones, versus aquellos preocupados por la búsqueda de oportunidades de desarrollo. Al aplicar una protección normativa a un determinado barrio, esto conlleva a que exista una serie de restricciones a la producción de obras nuevas, aspecto que complejiza -y muchas veces ahuyenta- los ánimos de ciertos desarrolladores. Sus efectos se reflejaron desde el 3º trimestre del 2016 al revisar una reducción de un 58% de los montos de inversión en la zona frente al mismo periodo en el 2017; y una notoria reducción de permisos ligados a patentes comerciales que ha relegado aquellos emprendimientos de tipo residencial, oficinas y educación. Frente a este comportamiento, el municipio santiaguino propuso no eliminar este tipo de acciones, sino regular la conformación de estas basándose en que este esfuerzo, significa a todas luces un aporte al contexto urbano histórico con el cual dialogar. Esta discusión escala en un contexto en el cual la demanda por vivir en áreas centrales va al alza, pero que reconoce desde el eje MATTA al sur un “territorio de especulación”. A simple vista se piensa en un desincentivo generalizado, toda vez existan tantas restricciones a la edificación continua en 18 metros como restricciones morfológicas en adyacencia a inmuebles de conservación histórica (ICH) que generan saltos en las rentabilidades resultantes. Sin embargo, esto puede ser reconocido como una oportunidad al aplicar estratégicamente los parámetros contenidos en la Zona de Conservación Histórica – E7 (ZCHE7) aprobada el 16 de agosto 2016 por el MINEDUC. La justificación final de esta tesis pretende desmitificar la postura que indica que la protección de los barrios congela su desarrollo inmobiliario, premisa que finalmente, modeló la hipótesis al buscar sus oportunidades dentro de radios acotados. Repensar más acuciosamente los nuevos productos para la zona se convierte en una oportunidad que brinda lineamientos con miras a generar nuevas tipologías y morfologías adaptadas al desarrollo local de barrio. / It’s all about interests exercised to the same urban space. Both heritage and real estate development correspond to study areas that are confronted nowadays, a logical situation if we think about certain heritage groups that preserve their constructions, versus those concerned in searching development opportunities. By applying a regulatory protection to a specific neighborhood, this regulatories lead to a series of restrictions applyed to the production of new urban pieces, an aspect that complicates – and oftently drives away - the spirits of certain developers. Its effects were reflected from the third quarter of 2016, when reviewing a reduction of 58% in investment amounts in the area compared to the same period in 2017; and a notable reduction in permits linked to commercial patents that has relegated residential, office and education ventures. Faced with this behavior, the city council of Santiago proposed not to eliminate this type of actions, but otherwise to regulate the conformation of these inititives, based on the fact that this effort, clearly means a contribution to the historical urban context with which to dialogue. This discussion escalates in a context in which, the demand for living in central areas of Santiago is constinously increasing, but which recognizes from MATTA axis to the south a “territory of speculation”. At first glance we think of a general disincentive, there are both restrictions on continuous building in 18 meters and morphological restrictions in adjacency to historical preservation buildings that generate leaps in the resulting returns. However, this can be recognized as an opportunity to strategically apply the parameters contained in the Historic Conservation Zone - E7 (ZCHE7 in spanish) approved on August 16, 2016 by MINEDUC. The final justification of this thesis aims to demystify the position that indicates that the protection of neighborhoods freezes their real estate development, a premise that finally modeled the hypothesis when looking for opportunities within limited radius. Rethinking new products more acutely for this area becomes an opportunity that provides guidelines that direct the generation of new typologies and morphologies adapted to local neighborhood development.

This thesis consists of five articles mainly devoted to problems in dynamical systems and ergodic theory. We consider non-uniformly hyperbolic two dimensional systems and limit distributions of point measures which are absolutely continuous with respect to the Lebesgue measure. Let $f_{a_0}(x)=a_0-x^2$ be a quadratic map where the parameter $a_0\in(1,2)$ is chosen such that the critical point $0$ is pre-periodic (but not periodic). In Papers A and B we study skew-products $(\th,x)\mapsto F(\th,x)=(g(\th),f_{a_0}(x)+\al s(\th))$, $(\th,x)\in S^1\times\real$. The functions $g:S^1\to S^1$ and $s:S^1\to[-1,1]$ are the base dynamics and the coupling functions, respectively, and $\al$ is a small, positive constant. Such quadratic skew-products are also called Viana maps. In Papers A and B we show for several choices of the base dynamics and the coupling function that the map $F$ has two positive Lyapunov exponents and for some cases we further show that $F$ admits also an absolutely continuous invariant probability measure. In Paper C we consider certain Bernoulli convolutions. By showing that a specific transversality property is satisfied, we deduce absolute continuity of the to these Bernoulli convolutions associated distributions. In Papers D and E we consider sequences of real numbers in the unit interval and study how they are distributed. The sequences in Paper D are given by the forward iterations of a point $x\in[0,1]$ under a piecewise expanding map $T_a:[0,1]\to[0,1]$ depending on a parameter $a$ contained in an interval $I$. Under the assumption that each $T_a$ admits a unique absolutely continuous invariant probability measure $\mu_a$ and that some technical conditions are satisfied, we show that the distribution of the forward orbit $T_a^j(x)$, $j\ge1$, is described by the distribution $\mu_a$ for Lebesgue almost every parameter $a\in I$. In Paper E we apply the ideas in Paper D to certain sequences which are equidistributed in the unit interval and give a geometrical proof of an old result by Koksma.