DIPLOMA THESIS: The impact of acupuncture on heart rate variability in patients with migraine SOCIAL AND CLINICAL PHARMACY DEPARTMENT, FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ, CHARLES UNIVERSITY IN PRAGUE AUTHOR: Kateřina Jehličková SUPERVISOR: PharmDr. Jitka Pokladníková, Ph.D. INTRODUCTION: Migraine is one of the most common diseases that affects up to 25% of human adult worldwide. The popularity of alternative therapeutic method is increasing, in this case namely acupuncture, currently between 11-46% globally, 4-9,9% respectively. OBJECTIVE: The primary goal of this work is to evaluate if acupuncture has any effect on HRV in patients with migraine. Secondary goal is to find out which psychosocial factors may influance HRV parameter changes. METHODOLOGY: HRV measurment was performed before acupuncture and after 12 weeks of acupuncture therapy. At first, results were processed in KUBIOS software which is specially designed for the study of heart rate variability and the needed parameters. Then the statistical analysis of a paired t-test (p=0,05) and linear regression (p=0,1) were done in SPSS software. MIDAS questionnaire (quality of life in patients with migraine), life satisfaction questionnaire (Satisfaction with Life Scale, SWLS) and The Symptom Checklist (SCL-90) were used to determine factors...

Cyclical component of aggregate net inventory investment in Czech republic between I.quarter 1996 and IV.quarter 2010 can be described by Production smoothing model, which says, that it is more advantageous for firms to absorb shocks of their product demand into inventories than to adjust their production permanently. This statement stands on a discovery, that cyclical component of net inventory investment is negativelly corralated with cyclical component of final sales and at the same time variability of cyclical component of final sales is higher than cyclical component of production (GDP). Although it is not possible to expect that every firm in the economy will behave according to this model, it seems, with respect to the analysis, that the cost of adjustment is one of the most important factors considered by firms within their optimalization problem.

Reuse is considered as one of the most crucial software engineering concerns. It allows for delivering software systems faster with less effort. Therefore, the thesis explores limits of reuse in the context of component systems. It analyzes in depth contemporary component systems, finds their commonalities and variation points, and introduces a meta-component system -- a software product line which allows for producing a tailored component system based on a set of requirements. The thesis addresses the meta-component system definition and focuses on its crucial aspects which play the key role in component systems preparation- (1) a configurable execution environment and (2) generation of implementation artifacts. To address the first aspect, the thesis proposes a model-driven method for creating configurable execution environments. Motivated by creating execution environments, the thesis contributes to (2) by introducing a notion of domain-specific languages interoperability in the context of the code generation. Furthermore, the thesis elaborates the proposed notion resulting into a family of interoperable domain-specific languages which is parametrized by a general purpose language.

The thesis deals with algorithms for detecting anomalies in the data collected by the Problem Solving Tutor research. In the theoretical part, the author introduces the term of anomaly, the ideas of the PROSO research and a detailed overview of existing algorithms to detect anomalies. In the practical part, selected algorithms are implemented. Real outputs and results as well as recommendations to a user are presented in this part, and the chapter is supplemented by a number of graphs. The implemented algorithms are also compared to existing data mining software. An example of working with the data mining tools, applied to the data coming from PROSO, and explanation of their outputs is also part of the thesis. In the summary, the appropriate methodology for behavior analysis and anomaly detection is determinied.

This work was the structure of resonant spruce wood devoted to. The tree-ring widths and summer wood measure were measured. By use of non - destructive methods their acoustic properties were measured - speed of sound, acoustic constant, logarithmic decrement of damping, acoustic wave resistance, wood - density and Young's modulus. Data were statistically analyzed and the wood structure coefficient of variation for each specimen was calculated. There were three different methodologies for verification of the wood structure variability effect formulated. Subsequently, it was found that it is possible to determine the effect on the acoustic properties of the wood macroscopic structure variability.

Variability in the genetic information DNA tracking individuals carry is easy to detect using molecular markers. In the thesis we examined related Dracaena species. For the study of genetic diversity in the genus Dracaena are used mainly noncoding regions of cpDNA, spacer trnH -- psbA, regions trnL -- trnF and trnS -- trnG -- trnG, which are more variable than coding regions. Also used in this work were coding regions of matK and rbcL. In the genus Dracaena belongs xerophytic species that are characterized by typical shaped treetop. 14 representatives of species were used in thesis growing in tropical regions of the African continent and adjacent islands and the southeastern part of the Arabian peninsula. They are monocotyledonous trees with atypical abilities of secondary thickness of trunk, which I find interesting. The massive trunk is very strong and there is potencial to used it for wood. Trees are very significant for their red plant sap which flowing from demaged trunk. The sap is very precious resource which is used in many areas of industry, for example pharmacy, traditional medicine, dye making etc. Determination of genetic affinity was based on an amplification of cpDNA template of individual Dracaena samples with primers for the studied regions. Data was obtained and evaluated by Multiple alignment program ClustalX and BioEdit after their sequencing. Evalueted data was used to create dendograms affinity. According the resulting phylogenetic tree we find out similarities and identified relationship of the monitored species of the genus Dracaena. The main purpose of research was to get answers to understand phylogenetic relationship between group of Dracaena forestry used trees. The thesis was made in cooperation of Department of Forest Botany, Dendrology and Geobiocoenology, Faculty of Forestry and Wood Technology of Mendel University in Brno.

The thesis deals with the variability of spelling of English loanwords in the Czech language, both from the diachronic and synchronic point of view. It explores Anglicisms in the Czech vocabulary in general, and focuses on the borrowings and their spelling variants occurring in recent journalistic texts. It also examines how the original spelling and pronunciation influence the final form of the loanword which eventually becomes standardised in Czech. The theoretical part describes the phonetic changes that take place when a word is borrowed and also the differences in the phonological systems of Czech and English. The practical part deals with competing alternatives of English loanwords in Czech, both those already firmly established and the newly borrowed ones, which are more likely to occur in dual spelling in the current Czech language.

Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple chlorotic leaf spot virus (ACLSV) are economically the most important viruses in pome fruit trees, which are distributed worldwide and can caused significantly yield reduction. The major control strategies (namely pathogen detection, exclusion by crop certification or quarantine, control in infected orchards by eradication from infected cultivars and rootstocks, etc.) rely heavily on accurate and sensitive detection methods and on perfect knowledge of pathogens. In the doctoral thesis the diagnostic method quantitative RT-PCR (qRT-PCR) was optimized for detection and quantification of four studied viruses. The results suggested that qRT-PCR method was the most reliable technique in comparison with conventional diagnostic methods DAS/I-ELISA and RT-PCR. In our study the concentration of ASGV, ASPV and ACLSV, measured by qRT-PCR, were stable during vegetation and in different plant tissue. Only the concentration of ApMV changed during vegetation in leaves and inner bark. This result indicates that changes of virus concentration observed by DAS/I-ELISA and RT-PCR in plant tissues are caused by other way (inhibitors, plant senescence, lower sensitivity, ect.) than by changes of virus concentrations in plant. Under the monitoring (at all 351 trees were tested) it was showed, that studied viruses were more spread in orchards and gardens then in wild apple trees. Selected virus isolates from wild apple trees and apples from orchards and gardens were sequenced and molecular variability was studied also with already published isolates. However individual isolates of studied viruses were similar. The variability associated with geographic origin or with type of planting has not been confirmed.

This thesis deals with the relationship between the place of origin and the extend of cold hardiness of fire bug Pyrrhocoris apterus (Linnaeus, 1758). Thirteen populations from twelve European localities were used in the experiment. Linear regressions were used to relate the values of SCP (10th percentile, first quartile, mean and median) of the individual populations to minimum and average winter temperatures of given localities. The relationship between air temperatures and SCP values were significant provided that the data of all populations were used. After the removal of three southern non-diapausing populations this relationship lost its significance.

Pisum fulvum Sibth. & Sm. is one of the three species in genus Pisum L., which also includes species Pisum sativum L. and Pisum abyssinicum A.Br. P. fulvum together with subspecies P. sativum subsp. elatius M.Bieb. and P. sativum subsp. humile Boiss. et Noe. belong to the group of wild pea. P. fulvum belongs to secondary gene pool of cultural pea P. sativum L. subsp. sativum. Due to the potential use of P. fulvum as a donor of gene of interest in processes of cultural pea genetic diversity enrichment, it may be considered as an important plant species whose biological characteristics should be assembled within the possibilities. Divergence separating P. fulvum from the rest of the evolutionary lineage of the genus Pisum occured in the period before 1.7 million years +/- 0.4 million years. Currently, its habitats is limited to the eastern part of the territory of Levant where P. fulvum is more abundant plant in its range than other groups of the genus Pisum that occurs sympatric with P. fulvum. The genetic diversity of P. fulvum is characterized in the current thesis by three approaches of molecular biology using DNA sequencing of ITS nuclear region and trnSG chloroplast region, DArT-SeqTM and GenoPea 13.2km SNP. Sequencing ITS region of 149 accessions led to the identification of four ribotypes that were analyzed in biogeographical context. Based on the comparing the analysis of data from genome-wide genotyping DArT-SeqTM and origin of analyzed accessions three groups (south, central and north) which can be considered as intraspecific evolutionary line were identified. In case of analysis of data from GenoPea 13.2K SNP P. fulvum genotype was compared with genotypes of other groups of pea and P. fulvum asociated SNPs were identified. Recent gene flow between some accessions of P. fulvum and accessions of other groups of wild pea was confirmed by analysis of codominant SNP data and by analysis of trnSG sequences.