The study arose out of a need to investigate and control a nosocomial outbreak caused by multidrug resistant Acinetobacter spp in the fifteen-bed intensive care unit of King Edward VIII Hospital. Following the discovery of the index case, four other patients were found to have a similar strain of Acinetobacter spp. All fifteen patients in the ward were subsequently screened for the organism. Forty-seven isolates were obtained from 12 patients. Eight of the patients were infected with the organism and six of these eight patients subsequently died. Swabs from the ward environment were also screened for the organism, which was found in patients' baths, suction water and urine collection jars. The outbreak was aborted by the use of strict infection control techniques. Minimum inhibitory concentrations (MICs) of 20 of the 47 isolates were determined for the following antimicrobials: imipenem, ciprofloxacin, gentamicin, amikacin, netilmycin,cefotaxime, ceftazidime and tetracycline. The same 20 isolates were further typed using ribotyping. Seven different antibiogram patterns were obtained using the MIC data. The majority of isolates (11) fit into a Single type, and showed resistance to all drugs tested, except for susceptibility to tetracycline and netilmycin only. Ribotyping revealed 5 different types. There were 9 isolates of ribotype a, 2 of ribotype b, 3 of ribotype c, 5 of ribotype d and 1 of ribotype e. In conclusion, this study describes a nosocomial outbreak with a multidrug resistant Acinetobacter spp. in an intensive care unit. The results showed that there was no correlation between the two typing methods used, ribotyping was more discriminatory than antibiogram types, with the majority of strains belonging to two different ribotypes. / Thesis (M.Med.)-University of Natal, Durban, 2000.

There have been many publications written about Henry VIII. It was his private life, because of which he primarily went down in history. Henry inherited the royal throne at a very young age. The Tudor dynasty ascended the English throne just for one generation. Henry's father ended the long−standing civil war. However, the fear of its continuation still remained. The most important task for the young king was to stabilize the position of the dynasty and to protect the throne against other pretenders. His whole life Henry lived in fear, that without a male heir, his family would be brought down. Catherine of Aragon became Henry's wife. There were many benefits from this union for England. The island kingdom was actively involved in continental policy. Nevertheless, Catherine was not able to give birth to an heir to England and fell into disgrace. Her nephew, Holy Roman Emperor, was the most powerfull ruler of the Christian Europe. Although he tried to prevent the annulment of their marriage by various means, he failed. Throughout his reign Charles was in a war conflict with the French king. He needed England like an ally against France even at the cost of his aunt's repudiation, which would remain without retaliation.

A presente pesquisa de doutorado pretende levantar, catalogar e examinar os vestígios arqueológicos dos contextos funerários, datados entre o intervalo do século XI ao VIII a.C. nos principais sítios da região da Argólida (Mapas 1, 2 e 3), Argos, Tirinto, Asine, Micenas, Náuplia e Lerna. Comparando tais dados entre si, e discutindo-os a partir de fundamentos teórico-metodológicos da Arqueologia das Práticas Mortuárias, objetivamos, por fim, levantar considerações sobre os costumes funerários dessa área, revelando possíveis padrões de enterramento e de comportamento sócio-cultural inseridos nas mudanças políticas ocorridas em um período de longa duração, principalmente, durante o período denominado de Alto Arcaísmo, o século VIII a.C., com o processo de formação da pólis argiva. / This thesis intends to gather, catalogue and analyze the archaeological data from the funerary contexts, dated from the XI to the VIII centuries BC in the main sites of the Argive plain (Maps 1, 2 and 3). Finally, it is also our aim to compare the data collected and discuss them through the theoretical and methodological basis of the Archaeology of Mortuary Practices trying to reach some questions and considerations about the funerary customs and burial patterns of this region and also possible social and cultural behavior characteristic of the shifts occurred mainly during the VIII century BC.

Neste trabalho foram estudados diversos métodos cromatográficos para a purificação de fatores recombinantes de coagulação VII (FVIIr) e VIII (FVIIIr) derivados de linhagens celulares humanas SK-Hep. O FVIIIr é utilizado para o tratamento da Hemofilia A, enquanto o FVIIr é utilizado para o tratamento da Hemofilia B e também a Hemofilia A. Produzir estes fatores em linhagens celulares humanas faz com os padrões de glicosilação, sulfatação e enovelamento destas proteínas sejam extremamente parecidos com os fatores endógenos produzidos no organismo humano. A purificação do FVIIIr através de técnicas de cromatografia multimodais usando a resina CaptoMMC, afinidade usando a resina FVIIISelect e troca iônica (SP-Sepharose) permitiu obter um produto bastante homogêneo e com perfil de banda (por SDS-PAGE) bem definido que demonstrou a presença esperada das cadeias leve e pesada (o Westen-Blott indicou que os anticorpos comerciais reconheceram a cadeia pesada da molécula estudada). As técnicas permitiram uma alta reprodutibilidade do processo onde sequencias de purificação indicaram o mesmo comportamento de perfis cromatográficos e o processo eliminou 99.5% ± 0,5% de proteínas inespecíficas, recuperando até 64% de FVIIIr. O FVIIr foi purificado com apenas uma única técnica cromatográfica usando a resina FVIISelect que isolou a proteína de interesse eliminando cerca de 99% de impurezas, recuperando praticamente todo o produto. O eluido da cromatografia de afinidade foi dialisado em membranas de 5 kDa o que resultou no processo de auto ativação da molécula de FVIIr, resultando em um aumento de sinal de até 5x em relação a quantidade inicial. O gel de SDS-PAGE e o Westen-Blott comprovaram o processo de auto-ativação no qual uma migração de banda de 50 kDa para 30kDa foi observada e os anticorpos comerciais contra FVII foram capazes de detecta-la. O método de purificação também foi bastante reproduzível e o perfil de banda muito semelhante se comparado ao produto comercial existente no mercado. Sendo assim, foi possível obter plataformas de purificação para as proteínas FVIIr e FVIIIr. / In this work, several chromatographic methods were studied for the purification of recombinant clotting factors VII (FVIIr) and VIII (FVIIIr) derived from human SK-Hep cell lines. The FVIIIr is used for the treatment of Hemophilia A, while the FVIIr is used for the treatment of Hemophilia B and Hemophilia A. Producing these factors in human cell lines results in glycosylation, sulphation and folding patterns similar to the endogenous factors produced in the human organism. Purification of FVIIIr by multimodal chromatography techniques using CaptoMMC resin, affinity using FVIIISelect resin and ion exchange (SP-Sepharose) yielded a fairly homogeneous and well-defined band profile (by SDS-PAGE) which demonstrated the expected presence of the light and heavy chains, Westen-Blott indicated that commercial antibodies recognized the heavy chain of the studied molecule. The techniques allowed a high reproducibility of the process where purification sequences indicated the same behavior of chromatographic profiles and the process eliminated 99.5% ± 0.5% nonspecific proteins and recovering up to 64% FVIIIr. FVIIr was purified with only a single chromatographic technique using the FVIISelect resin which isolated the protein by removing about 99% impurities and recovering virtually the entire product. The affinity chromatography eluate was dialyzed on 5 kDa membranes which resulted in the autoactivation process of the FVIIr molecule resulting in a signal increase of up to 5 fold over the initial amount. The SDS-PAGE gel and Westen-Blott demonstrated the auto-activation process where a migration of 50 kDa to 30 kDa band was observed and the commercial antibodies against FVII were able to detect the band. The purification method was also quite reproducible and the band profile very similar compared to the commercial products. Thus, it was possible to obtain purification platforms for the FVIIr and FVIIIr proteins.

Factor VIII is a clotting factor found on the intrinsic side of the coagulation cascade. A mutation in the factor VIII gene causes the disease Hemophilia A, for which there is no cure. The most common treatment is administration of recombinant factor VIII. However, this can cause an immune response that renders the treatment ineffective in certain hemophilia patients. For this reason a new treatment, or cure, needs to be developed. Gene editing is one solution to correcting the factor VIII mutation. CRISPR/Cas9 mediated gene editing introduces a double stranded break in the genomic DNA. Where this break occurs repair mechanisms cause insertions and deletions, or if a template oligonucleotide can be provided point mutations could be introduced or corrected. However, to accomplish this goal for editing factor VIII mutations, a way to deliver the components of CRISPR/Cas9 into somatic cells is needed. In this study, I confirmed that the CRISPR/Cas9 system was able to create a mutation in the factor VIII gene in zebrafish. I also showed that the components of CRISPR/Cas9 could be piggybacked by vivo morpholino into a variety of blood cells. This study also confirmed that the vivo morpholino did not interfere with the gRNA binding to the DNA, or Cas9 protein inducing the double stranded break.

No description available.

Thesis (DTh (Old and New Testament))--University of Stellenbosch, 2006. / This dissertation focuses on studying the relationship between humankind and nature in Psalm 8. The study of this relationship was triggered by Psalm 8 which, when closely examined, reveals a relationship suggesting an interrelatedness between humankind and nature. This research investigates the cause, nature, and result of that interrelatedness. The hypothesis that has guided this study has been that humankind and nature form a oneness or holism and partnership, and this oneness or holism and partnership lead to interrelatedness between these two entities. Our appreciation of this can be enhanced by approaching it from a Xhosa perspective. This approach also makes a contribution to Seventh-day Adventist theological reflection. In support of this hypothesis an exegesis of Psalm 8 is presented. Psalm 8 is one of the several Old Testament texts that seem to reflect this oneness and partnership, suggesting the interrelatedness between humankind and nature. In this exegesis a close reading approach was employed. The close reading approach refers to the studying of the language in the text, the style of the writing, the metaphors, and the relation of these to one another [Childs 1983:33, 34]. Xhosa and Seventh-day Adventist perspectives of the relationship between humankind and nature have been considered in this discussion: the Xhosa perspective because of my affiliation to these people, and also because Xhosas maintain that there is oneness or holism between humankind and nature. The Seventh-day Adventist perspective has been incorporated since I am a Seventh-day Adventist and have derived my theology from this tradition. Exegesis of Psalm 8 reveals a oneness or holism and partnership of humankind and nature, and this oneness or holism and partnership of these two entities leads to their interrelatedness. Moreover, this oneness and partnership that leads to the interrelatedness of the two entities is rooted in the creation of Genesis 1 of which Psalm 8 seems to be a development. In this relationship each party (humankind and other created beings) plays a role. Humans play the role of leading and representing the group in such a way that their actions or the results of those actions – good or bad - influence all members of the partnership. On the other hand, the other members of this partnership are there to serve the rest of the body, including humanity. In short, the emphasis on the relationship of Psalm 8 is that humankind and nature are so connected that they can hardly survive without each other. The benefit of this research is two-fold. First, it has provided an alternative perspective into the modern theological debate of the relationship of humankind and nature based on the Old Testament. This alternative is that humankind and nature form oneness or holism. Therefore, rather than separating humankind from nature and focusing the attention on humanity to the exclusion of nature, let the debate recognize this oneness and inseparableness.

This study explores the different ways in which music of the early-Tudor period can be analysed. Approaching the analysis first from a performer’s perspective, it takes the surviving works of Robert Fayrfax (1464–1521) as a case study. Fayrfax was chosen both because of the important rôle he plays within the chronology of changing style in early Tudor England, and because of the lack of a convincing analytical survey of his surviving works. Various analytical methods are developed by drawing upon three areas of investigation: (1) previous analyses of renaissance polyphony; (2) sixteenth-century music theory; and (3) hermeneutics. The basic issues and problems encountered when approaching early Tudor works from an analytical perspective are addressed in the Preface, and discussed in more detail in Chapters 1-3. These chapters form a theoretical basis for the work as a whole. Chapters 4-8 provide a detailed analytical interrogation of Fayrfax’s works, addressing five areas of investigation: the selection and development of pre-compositional material; rhythm and metre; mode and cadential planning; texture and tessitura; and motif, imitation, and free counterpoint. Whilst this study focuses specifically on the works of Robert Fayrfax, it is hoped that works by other early Tudor composers can also be examined using the analytical methods developed.

Memoria para optar al título de Ingeniero Civil Mecánico / En Chile y en gran parte del mundo se utiliza el código ASME VIII, para el diseño, fabricación y montaje de recipientes a presión. Sin embargo, las reglas de montaje del piping, no suelen ser respetadas, provocando fallas en los equipos. Esto es de suma importancia en ingeniería ya que las empresas fabricantes son las responsables de entregar la memoria de cálculo correspondiente. En base a esto, el objetivo general de esta memoria consiste en calcular la capacidad de carga en las boquillas de recipientes a presión, debido a fuerzas externas. Para esto, se deben desarrollar dos procedimientos de análisis: El WRC 107 y una técnica FEM. Los cuales son aplicados en un caso base y en un conjunto de aplicaciones. De esta manera, poder realizar una comparación entre los resultados de ambos análisis. Para lograr los objetivos, se evalúan 3 tipos de cargas: Una carga radial (P), un momento circunferencial (Mc) y uno longtudinal (Ml). Se aplica la metodología del WRC 107 imponiendo la condicion del límite de fluencia del material. Mientras que el FEM (Ansys), consiste en encontrar el instante en donde comienza la deformación plástica, debido a la carga aplicada. De esta manera se puede determinar de forma directa la magnitud. El resultado del caso base, muestra una mayor capacidad de carga en la boquilla de 10 in con respecto a la de 6 in, en ambos análisis (WRC 107 y FEM). Sin embargo, la diferencia entre cada uno de ellos es aproximadamente de un 60 %. Esto se debe a que WRC 107 no considera todos los refuerzos cercanos a la boquilla que presenta el caso base real. Los resultados correspondientes al conjunto de estanques seleccionados, muestran una tendencia a disminuir la capacidad de carga de las boquillas, en función del aumento del parámetro D=T del recipiente. Tanto para el análisis del WRC 107 como para el FEM. Esto muestra que el manto se comporta cada vez más similar a una membrana, perdiendo rigidez. También, se aprecia una diferencia entre ambos métodos de al menos un 29 %. Sin embargo, para la carga ML en la boquilla de 10 in, se presenta una diferencia de solo un 8 %. Se concluye que realizar el análisis de capacidad de carga en un caso real, conlleva a muchas consideraciones que no estan incluidas en el WRC 107, afectando considerablemente los resultados (WRC 107 hasta un 60% más conservativo que FEM). Para los casos parametrizados en funcion de D=T del estanque, se reafirma que WRC 107 es conservativo. Sin embargo, solo para la carga radial y momento circunferencial. No así para el momento longitudinal. Finalmente se destaca la importancia de realizar una técnica FEM para obtener resultados confiables.

Queen Anne Boleyn (~1507-1536) failed to meet social norms during her time as Queen Consort to Henry VIII (1491-1548). By tracing concepts of queenship through the works of Chrétien de Troyes, Andreas Capellanus, Thomas Malory, and Juan Luis Vives this thesis demonstrates how Anne united the office of queen and mistress to bring her downfall and introduce a new construct of queenship.