Thesis (D. Min.)--Perkins School of Theology, Southern Methodist University, 1985. / Abstract. Includes bibliographical references (leaves 147-150).

Orientador: Francisco Jorge Cividanes / Coorientador: Terezinha Monteiro dos Santos Cividanes / Banca:Eduardo Suguino / Banca: Daniel Junior de Andrade / Resumo: A nogueira-macadâmia (Macadamia integrifolia Maiden e Betche), originária da Austrália, encontra-se em expansão no Brasil. Por ser uma cultura recentemente introduzida no País, a maioria das informações e as tecnologias recomendadas para sua produção foram adaptadas de pesquisas efetuadas para as condições edafoclimáticas dos Estados Unidos e da Austrália. Esse fato ressalta a necessidade de se desenvolver estudos sobre a fauna entomológica, visando ao manejo integrado de pragas dessa nogueira. No presente estudo, foi realizado levantamento populacional de insetos em nogueira-macadâmia em Jaboticabal, São Paulo. O objetivo foi identificar as principais espécies de insetos fitófagos e predadores e determinar as espécies predominantes por meio de estudos de flutuação populacional desses organismos em relação aos estádios fenológicos da cultura. O total de 540 exemplares de insetos predadores foram coletados pertencentes a 8 ordens, 13 famílias e 37 espécies; os insetos fitófagos totalizaram 7588 exemplares de 5 ordens, 35 famílias e 83 espécies. Os predadores Chrysoperla externa (Hagen) (Neuropetra: Chrysopidae), Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), Allograpta hastata Fluke (Diptera: Syrphidae) e Nusalala sp. (Neuroptera: Hemerobiidae) e os fitófagos Toxoptera aurantii (Fonscolombe) Hemiptera: Aphididae), Frankliniella gardeniae Moulton (Tysanoptera: Thripidae), Haplothrips gowdeyi (Franklin) (Tysanoptera: Phlaeothripidae), Mormidea sp., Piezodorus sp. (Hemip... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The macadamia nut (Macadamia integrifolia Maiden e Betche) is native from Australia and is in expansion in Brazil. It was recently introduced in the country, therefore, most of the knowledges and technologies recommended for production were adapted from researches conducted for soil and climatic conditions in United States and Australia. This fact highlights the importance of studies about the entomological fauna, aiming at the integrated pest management of this walnut. In this study was conducted a population survey of insects in macadamia nut in Jaboticabal, São Paulo. The objective was to identify the main species of phytophagous insects and predators and to determine the predominant species through the study of its population dynamics in relation to the phenological stages of the crop. A total of 540 predatory insects specimens belonging to eigth orders, 13 families and summing up 37 species were collected. A total of 7588 specimens of phytophagous insects from five orders, 35 families and and summing up 83 species were captured. The insect predators Chrysoperla externa (Hagen) (Neuropetra: Chrysopidae), Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), Allograpta hastata Fluke (Diptera: Syrphidae) e Nusalala sp. (Neuroptera: Hemerobiidae) and the insect phytophagous Toxoptera aurantii (Fonscolombe) Hemiptera: Aphididae), Frankliniella gardeniae Moulton (Tysanoptera: Thripidae), Haplothrips gowdeyi (Franklin) (Tysanoptera: Phlaeothripidae), Mormidea sp., Piezodorus sp. (Hemiptera: Pentatomidae), Zicca sp. (Hemiptera: Coreidae), Stenocoris sp. (Hemiptera: Alydidae), Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae), Charidotis marginella (Fabricius) (Coleoptera: Chrysomelidae), Ophiderma sp. (Hemiptera: Membracidae), Epitragus sp.3 (Coleoptera Tenebrionidae) e Ptilodactyla sp.2 (Coleoptera: Ptilodactylidae) were classified as... (Complete abstract electronic access below) / Mestre

Master of Science / Food Science Institute / Kadri Koppel / Black walnut (Juglans nigra L.) is a Juglans species native to the United States. Nuts are collected each fall from black walnut trees and the kernels are consumed in many food products like ice cream, candies, and baked goods. Flavor profiles of black walnut cultivars have been examined, but no studies have looked at the effect of growing season on flavor profile, and few studies have determined consumer acceptance of black walnut food products. The sensory profiles of 10 black walnut cultivars (Football, Vandersloot, Brown Nugget, Pounds, Sparks 127, Davidson, Sparrow, Neel, Emma K, and Tomboy) were evaluated using descriptive sensory analysis. A trained panel scored the intensity of 3 appearance, 7 aroma, 23 flavor, and 6 texture attributes. Results showed that the cultivars differed significantly (P≤0.05) on 11 of these attributes. The results from this study were also compared to results collected in 2011 of 7 black walnut cultivars. Two flavor attributes (black walnut ID and overall nutty) had an interaction effect of year and cultivar, while 7 attributes showed a main effect of year (brown, caramelized, floral/fruity, fruity,-dark piney, musty/dusty, and oily). In general, flavor attributes had higher intensities in 2011 than in 2013. Six of the black walnut cultivars were also incorporated into a gelato base and evaluated by both a trained panel and consumer panel. Trained panelists developed a lexicon for the gelato samples and scored the intensity of 18 flavor attributes. The gelato samples differed on 3 main flavor attributes: black walnut ID, overall nutty, and sour (P≤0.05). Based on consumer liking, there were 3 distinct clusters of consumers. One cluster preferred samples with a milder black walnut flavor, another preferred a more intense black walnut and overall nutty flavor, and the third cluster liked all of the samples. Results from this study indicate that growing season should be considered when determining flavor profile of agricultural products. These results can also help guide growers in selecting cultivars that may produce a more consistent crop year after year, and cultivars that consumers find acceptable in food products.

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Air drying of naturally infested soils (0.12 to 0.38% water or about -2,000 bars) resulted in no recovery of Cylindrocladium crotalariae microsclerotia, but rewetting soils to near field capacity for 1 to 4 wk before assay resulted in partial recovery from the air drying-induced decreased germinability. Numbers of germinable C. crotalariae microsclerotia (assayed at 26 C) decreased progressively over 4 wk when naturally infested soils were incubated at 6 C. No germinable microsclerotia were recovered when soils were incubated at -10 C. When soils incubated at -10 C and at 6 C were transferred to 26 C for 4 wk, the low-temperature effect was partially reversed. Incubation of naturally infested soils under field conditions over the winter months (November-February) indicated that a similar low-temperature phenomenon exists in nature. Germinability of axenic, laboratory-grown microsclerotia of C. crotalariae, C. floridanum, and C. scoparium incubated 4 wk at 6 C ranged from 0 to 91.3% (mean = 37.7%) of the initial germinability. Partial recovery of laboratory-grown microsclerotia from the lowtemperature effect, by incubation at 26 C, was demonstrated. Conductivity measurements of solutions bathing microsclerotia incubated at 6 C and 26 C for 4 wk indicated that chilling injury may account, in part, for decreased germinability of microsclerotia. Direct observation of washed conidia of C. scoparium on rewetted, non-sterile soils at 26 C indicated that peak germination (33-58%) occurred after 24 h incubation. Peak germination on continually moist soils was somewhat lower (18-26%) than on rewetted soils. Conidia did not germinate on continually moist soils at 6 C. Conidia germinated at a high level (93-95%) in axenic culture in the absence of exogenous carbon and nitrogen substrates. The inhibition of conidium germination on soils was due, in part, to the presence of fungistatic soil volatiles. Addition of low levels of carbon and nitrogen substrates nullified the inhibitory effect of soil volatiles. Germinability of C. scoparium, C. crotalariae, and C. floridanum conidia in artificially infested soils (assayed on a selective medium at 26 C) decreased progressively during incubation at 26 C from 1 wk to 4 mo. No germinable conidia were recovered after incubation of soils at 6 C for 4 wk. Control of Cylindrocladium root rot of black wainut seedlings with sodium azide at 224 kg/ha applied by the plowdown method was comparable to MC-33 at 504 kg/ha. Sodium azide at 67 kg/ha was only marginally effective in disease control. Reduction of Cylindrocladium microsclerotium populations, qualitatively assayed by the azalea leaftrap method, was found in soil samples from plots fumigated with sodium azide and MC-33. A small-spored Cylindrocladium sp. with clavate to papillate vesicles, identified as C. parvum, was consistently recovered from diseased roots and soil samples from a Virginia forest nursery. Percentage recovery from necrotic roots and soil samples collected from root zones of necrotic black walnut seedlings was appreciably higher for C. parvum than for C. floridanum or C. scoparium. In greenhouse tests, limited pathogenicity of C. parvum on black walnut seedlings was demonstrated. / Ph. D.

Integrating trees into pasture may be an effective management tool to improve water, nutrient, and light allocation and increase total system productivity in Appalachia. We tested this hypothesis in a silvopasture near Blacksburg, VA. In 1995, black walnut and honey locust trees were planted within plots (r=3) of predominantly tall fescue pasture. Across a 12% slope, trees were planted to create treatments of low, medium, and high tree densities at shoulder, mid, and toe slope positions within plots of honey locust and black walnut. Sampling sites (n=54) under tree density and slope position combinations were harvested May to October at 35-d intervals in 2002 and 2003 for determination of yield and nutritive value characteristics. Soil surface temperature, forage canopy temperature, soil moisture, and photosynthetically active radiation were measured to determine forage responses to field treatments as functions of resource allocation. Tree density had the greatest effect on forage production and nutritive value. Across both years, yields were 16% greater (P=0.0006) at medium density (6130 kg/ha) compared to forage mass at low (5280 kg/ha)) and high density (4970 kg/ha, SE=130). Increasing tree density did not affect (P>0.2) ADF, CP, P, K, and Mg levels, but reduced (P<0.001) NDF and TNC, and increased (P<0.0001) ADL and Ca. Elevated soil surface and forage canopy temperatures limited forage production and nutritive value at low density, while low light levels were the limiting factor at high density. Moderating forage microclimate with appropriately spaced trees is an effective way to improve forage production in temperate pastures. / Master of Science

Thesis (MSc)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: The aim of this study was to combine two common diagnostic tools: serological kits and genetic fingerprinting to identify cherry leaf-roll nepovirus (CLRV), and to establish a marker system to characterize walnut germplasm. The detection of plant viruses is difficult. Restrictions are imposed for quarantine purposes on the importation of plant material from foreign countries. Modern techniques such as a PCR based screening method for CLRV are required to ensure material do not harbour viruses. A primer pair was designed to amplify a 430 bp non-coding homologous region. For the choice of primers, consensus sequences were considered and areas where the sequence data shared 98.5% homology, were chosen. The sensitivity of this detection method was 100-fold higher when compared to the ELISA. The PCR fragment was verified by nucleotide sequencing. AFLP technology was used to identify polymorphic fragments for 6 walnut cultivars and a rootstock, and SCARs were developed from AFLP specific bands. The AFLP technique distinguished all the walnut cultivars and the rootstock. However, conversion of AFLP fragments to SCAR markers for the development of a simple robust technique for cultivar discrimination, was not successful. Using 27 AFLP primer combinations, polymorphic fragments as high as 47.8% were scored. The reason for the lack of efficient conversion was as the result of the AFLP technique. The SCAR primers were generated from sequences internal to the AFLP primers but the specificity of the markers was in the AFLP primers not the internal sequence. In this study using AFLP, walnut cultivars were found to be closely related. The AFLP primer pairs used, provided polymorphic fragments. From these fragments, 7 SCAR markers were developed. It was expected that these SCARs derived from the AFLP markers would detect slight differences between cultivars. The Paradox SCAR marker was the only one that could divide the cultivars into two groups. When Chandler SCAR products were digested with the restriction enzyme Rsal, the same banding pattern as that of Paradox SCAR products was observed. / AFRIKAANSE OPSOMMING: Die doel van hierdie studie was om twee algemene opsporingstegnieke te kombineer: serologiese toetsstelle en genetiese vingerafdrukke om cherry leaf-roll nepovirus (CLRV) te eien en om In merkersisteem te ontwikkel wat okkerneut kiemplasma kan karakteriseer. Die opsporing van plant virusse is baie moeilik. As gevolg van kwarantyn vereistes, word daar beperkinge geplaas word op die invoer van plant materiaal vanuit die buiteland. Moderne tegnieke soos hierdie een wat op PKR berus, word benodig om te verseker dat CLRV nie in plantmateriaal teenwoordig is nie. In Stel inleiers is ontwerp wat In 430 bp nie-koderende homoloë area amplifiseer. Hiervoor is konsensus volgordes bestudeer en slegs die volgordes wat 98,5% homologie getoon het, is gekies. In vergelyking met ELISA was die sensitiwiteit van hierdie deteksie metode 100 maal beter. DNA volgordebepaling is op die resulterende fragment gedoen om die PKR produk te verifieer. AFLP tegnologie is gebruik om polimorfiese fraqmente vir 6 okkerneut kultivars en 'n onderstok te identifiseer en SCARs is uit hierdie fragmente ontwikkel. Die AFLP tegniek kon tussen al die okkerneut kultivars en die onderstok onderskei. Die omskakeling van die AFLP fragmente in SCAR merkers om sodoende In eenvoudige kragtige tegniek vir kultivar onderskeiding te ontwikkel, was egter nie suksesvol nie. Met die gebruik van 27 AFLP inleier kombinasies, kon polimorfiese fragmente van so hoog as 47.8% verkry word. Die rede hoekom omskakeling onsuksesvol was lê by die aard van die AFLP tegniek. Die SCAR inleiers is ontwikkel uit volyordes intern tot die AFLP inleiers, maar die spesifisiteit van die merkers het juis in die AFLP inleiers gelê en nie in die interne volgordes nie. In hierdie studie, met die gebruik van AFLP, is gevind dat okkerneut kultivars baie naby verwant is. Die AFLP inleierstelle wat gebruik is, het polimorfiese fragmente gelewer. Uit hierdie fragmente is 7 SCAR merkers ontwikkel. Daar is verwag dat die SCARs wat uit die AFLP merkers ontwikkel is, klein verskille tussen kultivars sou opspoor. Dit was egter net die Paradox SCAR merker wat die kultivars in twee groepe kon verdeel. Restriksie ensiem vertering met Rsalop die Chandler SCAR produkte het dieselfde bandpatrone as die van die Paradox SCAR produkte gelewer.

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Under Cooperative Agreement Number H8601010007, BARA contracted with the NPS to amend the document review with primary data from representatives of the tribes that were found to be historically and culturally affiliated with Sunset Crater Volcano, Walnut Canyon, and Wupatki National Monument. Additionally, this addendum to the affiliation study includes information about traditional uses of park resources as described by tribal representatives. This information is critical to park management and for compliance with various laws, regulations, executive orders, and policies so that park managers can better address tribal requests for continued access and use of park resources. The first purpose of this study is to amend the completed literature search with primary data collected with tribal representatives of the six ethnic groups. This data will provide contemporary validation of the literature search and contribute additional information related to tribal affiliation. The second purpose of this study is to provide primary data about past and present tribal uses of park resources. As a Traditional Use Study of park resources, this effort contributes information essential to park management as well as to compliance with a myriad of laws, regulations, executive orders, and NPS policies. The specific objectives of this study include identification of natural resources at each park that are used for traditional purposes, descriptions of the cultural importance of these resources, and, where possible, links between contemporary resource use and records of historic use.