Fish predation can have structuring effects in aquatic communities, but the most important fish predators are not always immediately obvious. Generalist fish predators often occupy similar habitats and consume similar prey making determination of their consumptive impacts difficult. Understanding these consumptive impacts is important for understanding complex wetland food webs. I collected warmouth (Lepomis gulosus), bluespotted sunfish (Enneacanthus gloriosus), and dollar sunfish (Lepomis marginatus) in two seasons from sloughs for both diet and bioenergetics analysis. Macroinvertebrates dominated diets of the three species, and nonparametric analyses revealed evidence of diet ontogeny in warmouth and potential competition for prey items among gape-matched individuals. Bioenergetics modeling revealed high levels of macroinvertebrate comsumption by these species relative to macroinvertebrate reproductive output suggesting that when combined with other sources of mortality, consumptive pressures placed by sunfish on benthic macroinvertebrates may be quite large. / by Jacob Bransky. / Thesis (M.S.)--Florida Atlantic University, 2012. / Includes bibliography. / Mode of access: World Wide Web. / System requirements: Adobe Reader.

Rapid bioassessment techniques used for aquatic macroinvertebrate and plants in wetlands were tested in New South Wales, Australia. Wetlands surveyed ranged from coastal wetlands in the North and Central parts of the State, to tableland wetlands west of Sydney. Wetlands varied in dominant vegetation, hydrology, substrate and level of human impairment. Different options for sampling (mesh size, live-picking times, quadrat size, number of samples or quadrats) and analysis (taxonomic resolution, transformations, biotic indices, multivariate and univariate analyses) are compared to determine optimal sampling effort and evaluate the effects of errors or variability. Results show that, for wetlands of New South Wales, sampling procedures developed and tested in streams and other regions of Australia may not be the most efficient. Using the data from 21 wetlands in New South Wales, a number of analytical techniques were evaluated for the effects of errors. Results show that species-level multivariate analysis is more sensitive in detecting less obvious differences between wetlands (i.e., small effect sizes), while family-level analyses are more appropriate for large effect sizes. A modified waterplant index was developed that is simpler and has a wider application than the other Australian options available. Inherent problems in each index tested were addressed. The results show that the process of summarising a large amount of information into a single value will result in the loss of both information and variability between samples and this cumulative effect of error may effect the assessment of wetland condition. The practical outcome of this thesis is a set of standardised steps to assess wetland quality using biological assemblages. The results show that protocols and indices for rivers are not directly transferable to palustrine, vegetation dominated wetlands. I present protocols that are more appropriate to wetlands and recognise that each protocol would need to be adapted for each wetland type. Despite the need for flexible protocols, I promote the need for a standard approach to wetland sampling and the need for consideration of the effects of errors in sampling designs. This study highlights the need for more research on the response of specific stressors to wetlands flora and fauna. The results from this study also show that wetland macroinvertebrates and plant communities can be used as surrogates in multivariate analyses for detecting large differences between wetlands (wetland types) but that impact assessment requires more detailed investigations including species identification and careful consideration of the choice of reference and control sites. In conclusion I emphasise the need for scientific rigour in the use of biological indicators and consideration of the effects of errors and implications to sampling designs. / Doctor of Philosophy (PhD)

Runnelling is a popular method of physical habitat modification employed on saltmarshes to control pest mosquito populations. The runnelling method involves linking the tidal source to isolated mosquito breeding pools via shallow channels that enable slow water movement of low amplitude tides. Increased tidal flushing inhibits mosquito development. The range of organisms which inhabit saltmarsh are likely to be influenced by altered tidal conditions as they exhibit specific physicochemical requirements for feeding, burrowing or growth. The dynamic nature of saltmarsh may mean that changes to the tidal frequency of a particular region of the saltmarsh promotes extension of marine-like conditions. Because runnels increase the frequency of flooding tidal events in specific regions of the saltmarsh this study predicted that resulting changes would be evident in the physical conditions of saltmarsh substrate, in the transport of buoyant vegetative propagules, in the population characteristics of surface grazing snails and in the density and aperture of crab burrows after flooding and non-flooding tidal events. The physical impacts of runnelling were determined at three marshes which appeared similar in terms of topography, substrate and tidal conditions. Soil water content and consolidation were measured using two sampling protocols: a) comparisons between modified and unmodified shores; and, b) comparisons with increasing lateral distance across the shore from the runnel edge. At one marsh, moisture levels were significantly higher at runnelled than at unrunnelled sites when tides filled the runnels, but this pattern was not found at the other marshes. Soil consolidation was greater at higher shore heights, but was not different between runnelled and unrunnelled shores. Measurements at different lateral distances from runnels demonstrated higher moisture levels and lower consolidation up to 5 m from the edge but not further away. Groups of marked Avicennia marina propagules were released at the three runnelled saltmarshes during flooding and non-flooding tidal events. Groups of propagules released within 10 m of a runnel were always transported significantly further from the starting position and further up the saltmarsh shore after both flooding and non-flooding tides than any other groups. In addition, the pattern of stranding on saltmarsh for significantly different groups was closely associated with the path of runnel construction so that propagules were located either in the runnel or in depressions linked to the runnel that had been isolated mosquito-breeding pools prior to runnelling. It is likely that altered physical soil conditions significantly affected the distribution and size structures of Salinator solida and Ophicardelus spp. snails recorded at the three saltmarshes. The interaction of tidal period and the presence of a runnel contributed to patterns with significant differences between runnelled and unrunnelled regions of the marsh. Generally, the runnel population of snails exhibited flood-like features even during non-flood periods. The distribution and size classes of snails did not differ with lateral distance from runnels. The burrow characteristics of the crab Helograpsus haswellianus were compared to increase the accuracy of estimating abundance from burrow counts. Including only those burrows which were obviously maintained by resident crabs significantly increased the confidence limits of estimating crab abundance using only burrow density counts. This method was applied to runnelled and unrunnelled sites to assess any changes in the density of burrows associated with the presence of runnels. Again, it is likely that physical soil conditions resulting from increased tidal frequency at the runnel did influence crab burrowing with fewer small burrows being found at the runnelled site, low on the shore. In addition, mid- and large-sized burrows tended to dominate close to the runnel edge. Site-specific soil characteristics may help to explain the lack of continuity in patterns associated with runnel effects on non-target saltmarsh resources. While the runnel may increase the soil water content of clayey substrates at some sites it could also result in de-watering of porous sandy soils at other shores. This was evident in the structure of the snail population and distribution of crab burrows which appeared to reflect altered soil physical characteristics associated with the runnel. Runnelling does affect non-target organisms in saltmarsh. However, the scale of impact was usually locally restricted (< 10 m from the runnel edge). The fact that patterns were not recorded at all sites suggests that the influence of runnels is variable and limited by substrate and some biological conditions. Given the efficiency and popularity of runnelling as a physical control method for reducing pest vector mosquito habitat, this study found no evidence to suggest that its use should be discontinued on any ecological basis measured.

Conservation science in rivers is still lagging behind its terrestrial and marine counterparts, despite increasing threats to freshwater biodiversity and extinction rates being estimated as five times higher than in terrestrial ecosystems. Internationally, most protected rivers have been assigned reserve status in the framework of terrestrial conservation plans, neglecting catchment effects of disturbance. While freshwater conservation tools are mainly index based (e.g. richness, rarity), modern terrestrial and marine conservation planning methods use complementarity-based algorithms - proven to be most efficient at protecting a large number of taxa for the least cost. The few complementarity-based lotic conservation efforts all use broad river classifications instead of biota as targets, a method heavily disputed in the literature. They also ignore current condition and future vulnerability. It was the aim of this thesis to develop a framework for conservation planning that: a) accounts for the connected nature of rivers b) is complementarity based and uses biota as targets c) integrates current status and future vulnerability I developed two different approaches using macroinvertebrate datasets from Australia, Canada and the USA. The first new method was a site/based two-tiered approach integrating condition and conservation value, based on RIVPACS/AUSRIVAS � a modelling technique that predicts macroinvertebrate composition. The condition stage assesses biodiversity loss by estimating a site-specific expected assemblage and comparing it to the actual observed assemblage. Sites with significant biodiversity loss are flagged for restoration, or other management actions. All other sites progress to the conservation stage, in which an index of site-specific taxonomic rarity is calculated. This second index (O/E BIODIV) assesses the number of rare taxa (as defined by &lt50% probability of occurrence). Using this approach on a dataset near Sydney, NSW, Australia, I was able to identify three regions: 1) an area in need of restoration; 2) a region of high conservation value and 3) an area that had high conservation potential if protection and restoration measures could counteract present disturbance. However, a second trial run with three datasets from the USA and Canada highlighted problems with O/E (BIODIV). If common taxa are predicted at lower probabilities of occurrence (p&lt50%) because of model error, they enter the index and change O/E (BIODIV). Therefore, despite an attractive theoretical grounding, the application of O/E (BIODIV) will be restricted to datasets where strong environmental gradients explain a large quantity of variation in the data and permit accurate predictions of rare taxa. It also requires extensive knowledge of regional species pools to ensure that introduced organisms are not counted in the index. The second approach was a proper adaptation of terrestrial complementarity algorithms and an extension to the Irreplaceability-Vulnerability framework by Margules and Pressey (2000). For this large-scale method, distributions for 400 invertebrate taxa were modeled across 1854 subcatchments in Victoria, Australia using Generalised Additive Models (GAMs). The best heuristic algorithm to estimate conservation value was determined by calculating the minimum area needed to cover all 400 taxa. Solutions were restricted to include rules for the protection of whole catchments upstream of a subcatchment that contained the target taxon. A summed rarity algorithm proved to be most efficient, beating the second best solution by 100 000 hectares. To protect 90% of the taxa, only 2% of the study area need to be protected. This increases to 10% of the study area when full representation of the targets is required. Irreplaceability was calculated by running the heuristic algorithm 1000 times with 90% of the catchments randomly removed. Two statistics were then estimated: f (the frequency of selection across 1000 runs) and average c (contribution to conservation targets). Four groups of catchments were identified: a) catchments that have high contributions and are always selected; b) catchments that have high contributions and are not always selected; c) catchments that are always chosen but do not contribute many taxa; d) catchments that are rarely chosen and did not contribute many taxa. Summed c, the sum of contributions over 1000 runs was chosen as an indicator of irreplaceability, integrating the frequency of selection and the number of taxa protected. Irreplaceability (I) was then linked to condition (C) and vulnerability (V) to create the ICVframework for river conservation planning. Condition was estimated using a stressor gradient approach (SGA), in which GIS layers of disturbance were summarised to three principal axes using principal components analysis (PCA). The main stressor gradient � agriculture � classified 75% of the study area as disturbed, a value consistent with existing assessments of river condition. Vulnerability was defined as the likelihood that land use in a catchment would intensify in the future. Hereby current tenure was compared to land capability. If a catchment would support a land use that would have a stronger effect on the rivers than its current tenure, it was classified as vulnerable. 79% of catchments contained more than 50% vulnerable land. When integrating the three estimators in the ICV-framework, seven percent of catchments were identified as highly irreplaceable but in degraded condition. These were flagged for urgent restoration. Unprotected, but highly irreplaceable and highly vulnerable catchments that were still in good condition made up 2.5% of the total area. These catchments are prime candidates for river reserves. The ICV framework developed here is the first method for systematic conservation planning in rivers that is complementarity-based, biota-driven but flexible to other conservation targets and accounts for catchment effects, thus fulfilling all the gaps outlined in the aims.

Thesis (Ph.D.)--McMaster University, 2006. / Supervisor: Patricia Chow-Fraser. Includes bibliographical references.

Understanding the first order controls over resource cycling and limitation in ecosystems is critical for predicting ecosystem response to disturbances. Topography and vegetation self-organizing mechanisms are first order controls over resource fluxes across the landscape. Topography controls downslope flow of resources (i.e water and nutrients). Through spatial feedbacks, vegetation is able to actively modify its environment and maximize resource flows towards it. To date, the impacts of these controls on ecosystem dynamics have mostly been investigated separately. As such, there is a knowledge gap in the understanding of how these first order controls together dictate the dynamics of the ecosystem. This dissertation aims to gain a better understanding of how self-organizing mechanisms and topography operate together to affect wetland ecosystem dynamics. A spatially explicit, wetland vegetation patterning model that includes for both vegetation self-organizing control and topographic control is developed (Nutrient Depletion Model, NDM). The model describes a scale dependent feedback between vegetation, transpiration and nutrient accumulation that drives the formation of vegetation patterns. The model is applied to investigate the effects of topography and self-organizing mechanisms on form and orientation of vegetation patterns and vegetation growth dynamics of wetland ecosystems. Results show that the two first order controls synergistically impact the formation of the various patterns as observed in wetland ecosystems. Results also show the following: (1) Self-organizing mechanisms result in a more efficient retention of resources, which result in higher biomass in the model that include for both self-organizing mechanism and topographic control (SO+TC) than in the model that that includes only for topographic control (TC). (2) However, when resources or topographic gradients increase or annual rainfall decrease, the vegetation growth dynamics of the TC+SO and TC models converge. The NDM is applied to arctic Alaska to investigate how do the two first order controls impact present and future C-N dynamics of an arctic ecosystem. Simulation results show no significant difference in the dynamics between the SO+TC model and the TC model. The climate change simulation results suggest that changes in daily variability of temperature and precipitation can impact ecosystem dynamics as much as the changes in mean temperature and precipitation. Results from this dissertation provide a more complete picture on the relative roles of the two first order controls over ecosystem nutrient cycling and vegetative growth dynamics. Finally, in this thesis, in order to simulate small-scale feedbacks over large spatial domains, the NDM is implemented in a GPU computing language, which accelerates computational simulation by at least two orders of magnitude. These tools for grid-based simulations can provide a platform for using GPUs in other areas of scientific investigation.

Thesis (M.S.)--Mississippi State University. Department of Agricultural and Biological Engineering. / Title from title screen. Includes bibliographical references.

 This study primarily aimed to evaluate the usefulness of fatty acids (FAs) in revealing trophic relationships in Hong Kong wetlands, through a combination of field studies and laboratory experiments. A field-based study in Mai Po mangroves involved FA profiling of basal food sources (i.e., leaf litter from three mangrove species, diatoms and macroalgae, and sediments) and consumers (particularly crabs). FA composition of all mangroves was similar, and lacked some polyunsaturated FAs present in diatoms and macroalgae. Uca and Sesarma crabs, with different feeding mechanisms, had divergent FA profiles: Uca arcuata FAs reflected a diet of macroalgae and diatoms, while FAs of Sesarma spp. were typical of mangrove leaves. Temporal changes in consumer FA profiles between 2001 and 2007 appeared attributable to increased sedimentation at Mai Po and shifts in organic content of the substratum. A second field-based study was conducted at Luk Keng marsh where a salinity gradient (0 to 30?) allowed investigation of the effects of salinity changes in FA profiles and stable isotope (carbon and nitrogen) signatures of the consumers and their foods. Basal food sources were leaf litter, including a fungal biomarker of decomposition (ergosterol), fine particulate organic matter (FPOM) and periphyton. Both FPOM and periphyton (but not leaf litter) contained 20:4 and 20:5 FAs, but their concentrations were affected by salinity. FA 20:4 occurred at higher levels in samples from fresh water, whilst FA 20:5 exhibited the opposite pattern and was more abundant under saline conditions, and thus the ratio of FA 20:4 to FA 20:5 decreased with increasing salinity. Combined application of FA biomarkers and isotopic signatures were able to elucidate trophic relationships between consumers and their food at Luk Keng confirming that FA 20:4 as a useful biomarker in the freshwater portion and FA 20:5 in the more saline area. FA 20:4 was particularly associated with predatory freshwater insects that had high δ15N values, but was scarce in primary consumers (snails, detritivorous beetles) with low δ15N values. Two laboratory experiments were undertaken to investigate: 1) the effect of diet on FA profiles in the apple snail, Pomacea canaliculata, and 2) interacting effects of diet and salinity on FA profiles of the Indian medaka fish, Oryzias melastigma. The results of the apple snail study showed that dietary-mediated changes in FA profiles were only reflected in the snail tissues after at least three months, and FA profiles of digestive tissues and neutral lipids were first to respond to the dietary change. The results of the medaka study demonstrated that the ratio of FA 20:4 to FA 20:5 was affected by both diet and salinity, reflecting a similar finding in the Luk Keng field study, although diet had a stronger effect on this ratio. The results of both field studies supported the use of FA profiles as food web tracers in wetlands and were complemented by laboratory results that yielded insights which will allow refinement of FA biomarker applications in food-web studies. / published_or_final_version / Biological Sciences / Doctoral / Doctor of Philosophy

Agricultural practices have altered natural wetland habitats for thousands of years in lowland areas of Southeast Asia, and currently these highly modified wetlands constitute some of the most important remaining habitats for amphibians. However, decreasing area of arable land and increased use of chemicals may affect the persistence of lowland amphibian populations that are now dependent on these habitats. I investigated how amphibians responded to different kinds of farming treatments in a large agricultural wetland in Hong Kong. In the first part of the study, I assessed the occurrence of breeding amphibians in 53 farming plots in 2010 and 2011. Anuran call surveys were conducted at night at the plots once a week from March to August for two years. Environmental variables were measured and used to develop models to examine species presence, occupancy, and detection probabilities. Wet agricultural plots supported 10 species of amphibians and all had detection probabilities of < 1 that varied seasonally and yearly. Organically managed plots and shallow water plots yielded high species richness and particularly attracted the ornate pygmy frog (Microhyla fissipes) and the paddy frog (Fejervarya limnocharis). Air temperature and humidity were the relatively consistent predictors that influenced calling activity of the four most commonly detected species (M. fissipes; F. limnocharis; brown tree frog Polypedates megacephalus; and G?nther’s frog Hylarana guentheri). For the second part of the study, I assessed the impacts of fertilizers on amphibians. Using mesocosm experiments in the field, I compared the effects of a chemical fertilizer (granular urea) and an organic fertilizer (peanut cake) on the survival and growth of hatchlings of Polypedates megacephalus, the marbled pygmy frog (Microhyla pulchra), Asian common toad (Duttaphrynus melanostictus) and Chinese bullfrog (Hoplobatrachus rugulosus). Fertilizers were applied at low, manufacturer-recommended, and high levels, and survival and snout-vent-length were measured after 21 days. No individuals survived in the chemical fertilizer treatment at the recommended application level. Conversely, survival was high for P. megacephalus (96%), M. pulchra (54%) and D. melanostictus (90%), but relatively low for H. rugulosus (18%), at the recommended level of the organic fertilizer. P. megacephalus and M. pulchra tadpoles showed increased growth in elevated concentrations of organic fertilizer. Polypedates megacephalus tadpoles were 1.6 times longer in the low concentration and almost double in length in the high concentration treatments. Similarly, increased growth in M. pulchra in all organic treatments resulted in abbreviated time to metamorphosis. Chemical fertilizers are clearly detrimental to early life stages of these amphibians, but organic fertilizers may confer benefits including a shorter time to, and larger size at, metamorphosis. These results suggest that where amphibian conservation is a priority, shifts in the management of wet agricultural crops and limiting the use of chemical fertilizers may increase the suitability of breeding habitats and survival at early life stages. / published_or_final_version / Biological Sciences / Master / Master of Philosophy

The wetlands on the Eastern Shores of Lake St Lucia are primarily groundwater fed and exhibit a variety of hydrological regimes that give rise to a high degree of habitat and species diversity. Hydrologically unstable systems experience ecophasal shifts that can disrupt an established steady state within the wetland ecosystem. Communities of both plants and animals can accordingly disintegrate into more or less isolated populations, open to re-invasion by preceding or "new" species when conditions change again. Given the ephemeral and episodic nature of much of the surface water on the Eastern Shores, ecological dynamics of this type are likely. Fish and aquatic invertebrates were sampled from a number of routine and other sites between May 2002 and April 2003. Measurements of various environmental and abiotic factors (including pH, ionic conductivity and dissolved oxygen levels) were taken with each sample in order to establish relationships between environmental changes and the assemblages of aquatic fauna occurring within the Eastern Shores wetlands. Conditions on the Eastern Shores during the study were somewhat anomalous, as the region experienced drought conditions during this period. The Eastern Shores wetlands support a diversity of aquatic fauna, including at least four species of freshwater fish listed as rare or threatened by the IUCN. The aquatic organisms existing within this dynamic system exhibited changes in abundance and distribution that reflected the spatial and temporal changes in their environment. The relationships between aquatic organisms and their environment were complex, with assemblages being affected by combinations of changing environmental and habitat variables as well as other factors such as the environmental stability of habitats and stochastic effects. Given the complex nature of these interactions, aquatic macrofauna on the Eastern Shores are likely to be best conserved through the preservation a heterogeneous mix of wetland habitats, maintaining the diversity of wetland structure and function on the Eastern Shores that can facilitate an element of lottery in the development and structure in biotic assemblages. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2004.