Philosophiae Doctor - PhD / As a consequence of various cellar activities the wine industry produces copious volumes of potentially hazardous wastewater each year. South Africa is one of the top ten wine-producing countries, making the successful treatment of cellar effluent an important environmental obligation in this country. Constructed wetlands (CWs) are resilient to the seasonal input fluxes associated with agri-industrial waste and are ideal systems for the in-situ treatment of cellar effluent in small to medium-sized wineries not connected to municipal reticulation systems. In a project sponsored by the Water Research Commission of South Africa, a number of studies were undertaken to assess the remediation of winery wastewater and common components of winery wastewater in sand-filled pilot-scale constructed wetlands operated in batch mode. This thesis contains the results of three studies. The first study evaluated the temporal aspects of CW equilibration as a basis for future studies of system response to amendment. Microbial biomass and hydraulic conductivity values were monitored and microbial community fingerprints were obtained using denaturing gradient gel electrophoresis. The study showed that microbial community fingerprinting provides a valuable tool to assess the time-scales of microbial equilibration, which was found to be in the order of 100 days. In the second study, the biodegradation and mineralization of ethanol by acclimated and non-acclimated microbial populations in CWs were compared. By increasing the influent ethanol concentration at incremental intervals (incremental priming), the biodegradative capacity was significantly enhanced. At an influent COD concentration of 15 800 mg/L, no volatile fatty acids were detected in the effluent of an incrementally primed system and the maximum effluent COD measured was 180 mg/L. In contrast, an identical, unprimed system, amended with a lower concentration of COD (7587 mg/L), exhibited a maximum effluent COD concentration of 1 400 mg/L, with the metabolites butyrate and propionate accounting for up to 83% of the effluent COD. It was conclusively demonstrated that the use of incremental priming, together with the batch mode of operation enhanced long-term function of the CWs. In the third study, the removal of the phenolic component of winery wastewater was evaluated in CWs, as well as in sand columns and microcosms. It was found that at low influent phenolic concentrations in CWs, complete organic removal was accomplished, but at high concentrations, there was incomplete substrate removal and an accumulation of potentially toxic metabolites, including catechol. The sand provided a suitable substrate for the treatment of phenolic-laden waste, and both biotic (48%) and abiotic (52%) removal mechanisms effected the removal of model phenolics. Prior acclimation of microbial communities increased the biodegradation rate of phenolic acids significantly.

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Building structure of wine-growing is situated in the countryside not far from the village Strachotin in South Moravia. Concerned area slopes wildly down to the level of the water reservoir. To the building of production concures parts of presentation, degustation, and distribution of wine. This area includes also leisure centre with possibility of accomodation , visit of wellness, seminary rooms, restaurant and coffee. Buildings for accomodation and some other are designed as energetically selfsufficient wooden structures using energy from renewable natural resources.

This thesis is embodied by the design of a new gravity flow winery on the eastern slopes of the Blue Ridge Mountains in Northern Virginia. The 100 acre site is home to an existing winery with grape vines aged over a decade. From its initial inception, this thesis has been in part a response to the rapidly growing wine industry in Virginia and the typical ad hoc architecture associated with the wineries serving the state. The swelling interest in the culture of wine has sparked an evolution of the role of wineries. The winery of today is both an establishment for the production of wine as well as a venue for the enjoyment of its product and the delight of its natural setting. Creating a dialogue between site and visitor via thoughtful engagement of earth, air and sky is key for establishing a sense of place. The winery at the heart of this thesis is the quintessence of these ideals. The architecture of this winery embraces the steps of gravity flow winemaking, which aims to preserve the integrity of the grapes by utilizing gravity instead of electric pumps. The design seeks to make these steps perceivable and understandable, taking a visitor from the wind swept heights of the mountainside to the cool air of the winery's subterranean vaults and back again. From vine to glass and from layperson to student of wine, this shared journey engenders a transformation of both grape and visitor. / Master of Architecture

The topic of the diploma thesis is a multifunctional building of a hotel, in an undeveloped area of picturesque vineyards. The expanding Nikolsburg winery plans to build a hotel with a beautiful view of the historic town Mikulov and the Pálavské vrchy. Hotel should include accommodation for at least 30 guests in **** rooms, a restaurant, a wellness area and, above all, wine storage areas. The wine will be matured in the building and further distributed. Visitors can degustate and buy the wine here. The architectural concept further expands the multifunctionality of the assignment. Common areas should be adapted to the situations and the problem of one-time occupancy of the building, especially in the season and on weekends.

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The aim of this thesis is to create a business plan of a winery, evaluate its economic performance and present a valuation in order to offer a share of the company to the investor. The theoretical part covers mainly structure of the business plan, content of respective chapters, advantages and disadvantages of particular legal forms of companies and main economic indicators that are relevant for assessment of economic viability of the project. The practical part covers the actual set-up of the winery according to the business plan structure as well as a comprehensive financial plan. The economic viability and DCF valuation of the project is assessed based on this plan in order to offer a share of the company to the investor.

ST´WINERY - Winery is situated in the countryside near the village Strachotin. The lot strongly descend down to the Nové Mlýny Dam. It brings the possibility of beautiful views toward the Palava hills. The winery itself is in connection with the additional services - wellness, accomodation, restaurant, media. These services increase the attractiveness of the location and represent the missing functions in the area. They are destined to the recreation of the winery guests. For these purposes I designed a complex of four buildings which are connected via green intensive roof. It is covered by vineyard and this fact allows an effective use of the roof area. In addition, this fact possitively affects the inner climate inside the buildings. The design is inspired by the ground fissures. It is visible on the inner square between the objects. The complex is in accord with the standards of passive housing.

The goal of the thesis was to design a small vineyard in the vicinity of the Strachotín town, together with other functions (wine production, tasting, accommodation, sheepfold, cheese production and accommodation for winemakers). The basis for the area design comes from the concept of urban landscape, defining the landscape (function division, usability of the territory, views, …), as well as the placement of main structures (vineyard) and smaller accompanying constructions. The size and function of the vineyard (the core part of the thesis) are based on its requirements to be operational, as well as possibilities given by the location. The architecture strives to respect the location in order to create a harmony with the nature.