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Xeroderma Pigmentosa Group a (XPA), Nucleotide Excision Repair and Regulation by ATR in Response to Ultraviolet IrradiationMusich, Phillip R., Li, Zhengke, Zou, Yue 01 January 2017 (has links)
The sensitivity of Xeroderma pigmentosa (XP) patients to sunlight has spurred the discovery and genetic and biochemical analysis of the eight XP gene products (XPA-XPG plus XPV) responsible for this disorder. These studies also have served to elucidate the nucleotide excision repair (NER) process, especially the critical role played by the XPA protein. More recent studies have shown that NER also involves numerous other proteins normally employed in DNA metabolism and cell cycle regulation. Central among these is ataxia telangiectasia and Rad3-related (ATR), a protein kinase involved in intracellular signaling in response to DNA damage, especially DNA damage-induced replicative stresses. This review summarizes recent findings on the interplay between ATR as a DNA damage signaling kinase and as a novel ligand for intrinsic cell death proteins to delay damage-induced apoptosis, and on ATR’s regulation of XPA and the NER process for repair of UV-induced DNA adducts. ATR’s regulatory role in the cytosolic-to-nuclear translocation of XPA will be discussed. In addition, recent findings elucidating a non-NER role for XPA in DNA metabolism and genome stabilization at ds-ssDNA junctions, as exemplified in prematurely aging progeroid cells, also will be reviewed.
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A New Structural Insight Into XPA-DNA InteractionsHilton, Benjamin, Shkriabai, Nick, Musich, Phillip R., Kvaratskhelia, Mamuka, Shell, Steven, Zou, Yue 01 January 2014 (has links)
XPA (xeroderma pigmentosum group A) protein is an essential factor for NER (nucleotide excision repair) which is believed to be involved in DNA damage recognition/verification, NER factor recruiting and stabilization of repair intermediates. Past studies on the structure of XPA have focused primarily on XPA interaction with damaged DNA. However, how XPA interacts with other DNA structures remains unknown though recent evidence suggest that these structures could be important for its roles in both NER and non-NER activities. Previously, we reported that XPA recognizes undamaged DNA ds/ssDNA (double-strand/single-strandDNA) junctions with a binding affinity much higher than its ability to bind bulky DNA damage. To understand how this interaction occurs biochemically we implemented a structural determination of the interaction using a MS-based protein footprinting method and limited proteolysis. By monitoring surface accessibility of XPA lysines to NHS-biotin modification in the free protein and the DNA junction-bound complex we show that XPA physically interacts with the DNA junctions via two lysines, K168 and K179, located in the previously known XPA(98-219) DBD (DNA-binding domain). Importantly, we also uncovered new lysine residues, outside of the known DBD, involved in the binding. We found that residues K221, K222, K224 and K236 in the C-terminal domain are involved in DNA binding. Limited proteolysis analysis of XPA-DNA interactions further confirmed this observation. Structural modelling with these data suggests a clamp-like DBD for the XPA binding to ds/ssDNA junctions. Our results provide a novel structure-function view of XPA-DNA junction interactions.
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Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum ARajakulendran, Nishani 15 November 2013 (has links)
This project addressed mechanisms of the neuronal DNA damage response after treatment with the model DNA damaging agent ultraviolet light (UV). The thymidine analogue, 5-bromo-2’-deoxyuridine (BrdU) protected against UV-initiated neuronal apoptosis in a concentration-dependent manner (p<0.001). BrdU did not protect proliferating mouse embryonic fibroblasts from UV-induced apoptosis. We assessed whether the mechanism of BrdU neuroprotection was through a modification in the neuronal DNA damage response. BrdU neuroprotection was independent of BrdU incorporation into DNA, neuronal DNA repair, p53 activation or cell cycle re-entry, a neuronal DNA damage response. Neurons deficient in Cockayne Syndrome B (CSB) or Xeroderma Pigmentosum A (XPA) were paradoxically resistant to UV-initiated apoptosis. Therefore, CSB and XPA play essential roles in the neuronal DNA damage response.
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Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum ARajakulendran, Nishani 15 November 2013 (has links)
This project addressed mechanisms of the neuronal DNA damage response after treatment with the model DNA damaging agent ultraviolet light (UV). The thymidine analogue, 5-bromo-2’-deoxyuridine (BrdU) protected against UV-initiated neuronal apoptosis in a concentration-dependent manner (p<0.001). BrdU did not protect proliferating mouse embryonic fibroblasts from UV-induced apoptosis. We assessed whether the mechanism of BrdU neuroprotection was through a modification in the neuronal DNA damage response. BrdU neuroprotection was independent of BrdU incorporation into DNA, neuronal DNA repair, p53 activation or cell cycle re-entry, a neuronal DNA damage response. Neurons deficient in Cockayne Syndrome B (CSB) or Xeroderma Pigmentosum A (XPA) were paradoxically resistant to UV-initiated apoptosis. Therefore, CSB and XPA play essential roles in the neuronal DNA damage response.
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ATR-Dependent Checkpoint Modulates XPA Nuclear Import in Response to UV IrradiationWu, X., Shell, S. M., Liu, Y., Zou, Y. 01 February 2007 (has links)
In response to DNA damage, mammalian cells activate various DNA repair pathways to remove DNA lesions and, meanwhile, halt cell cycle progressions to allow sufficient time for repair. The nucleotide excision repair (NER) and the ATR-dependent cell cycle checkpoint activation are two major cellular responses to DNA damage induced by UV irradiation. However, how these two processes are coordinated in the response is poorly understood. Here we showed that the essential NER factor XPA (xeroderma pigmentosum group A) underwent nuclear accumulation upon UV irradiation, and strikingly, such an event occurred in an ATR (Ataxia-Telangiectasia mutated and RAD3-related)-dependent manner. Either treatment of cells with ATR kinase inhibitors or transfection of cells with small interfering RNA targeting ATR compromised the UV-induced XPA nuclear translocation. Consistently, the ATR-deficient cells displayed no substantial XPA nuclear translocation while the translocation remained intact in ATM (Ataxia-Telangiectasia mutated)-deficient cells in response to UV irradiation. Moreover, we found that ATR is required for the UV-induced nuclear focus formation of XPA. Taken together, our results suggested that the ATR checkpoint pathway may modulate NER activity through the regulation of XPA redistribution in human cells upon UV irradiation.
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Modula??o da express?o da prote?na XPA em resposta ao tratamento com azul de metileno fotossensibilizadoSilva, Acarizia Eduardo da 06 October 2010 (has links)
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Previous issue date: 2010-10-06 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Reactive oxygen species (ROS) are produced by aerobic metabolism and react with biomolecules, such as lipids, proteins and DNA. In high concentration, they lead to oxidative stress. Among ROS, singlet oxygen (1O2) is one of the main ROS
involved in oxidative stress and is one of the most reactive forms of molecular oxygen. The exposure of some dyes, such as methylene blue (MB) to light (MB+VL), is able to generate 1O2 and it is the principle involved in photodynamic therapy (PDT).
1O2 e other ROS have caused toxic and carcinogenic effects and have been associated with ageing, neurodegenerative diseases and cancer. Oxidative DNA damage is mainly repaired by base excision repair (BER) pathway. However, recent
studies have observed the involvement of nucleotide excision repair (NER) factors in the repair of this type of injury. One of these factors is the Xeroderma Pigmentosum Complementation Group A (XPA) protein, which acts with other proteins in DNA
damage recognition and in the recruitment of other repair factors. Moreover, oxidative agents such as 1O2 can induce gene expression. In this context, this study aimed at evaluating the response of XPA-deficient cells after treatment with photosensitized MB. For this purpose, we analyzed the cell viability and occurrence of oxidative DNA damage in cells lines proficient and deficient in XPA after treatment with MB+VL, and
evaluated the expression of this enzyme in proficient and complemented cells. Our results indicate an increased resistance to treatment of complemented cells and a higher level of oxidative damage in the deficient cell lines. Furthermore, the treatment was able to modulate the XPA expression up to 24 hours later. These results indicate
a direct evidence for the involvement of NER enzymes in the repair of oxidative damage. Besides, a better understanding of the effects of PDT on the induction of gene expression could be provided / Esp?cies reativas de oxig?nio (ERO) s?o produzidas durante o metabolismo aer?bico e s?o capazes de reagir com diversas biomol?culas, como lip?dios, prote?nas e DNA. Dentre as ERO, o oxig?nio singlete (1O2) e conhecido como um dos principais agentes envolvidos no estresse oxidativo. A exposi??o de alguns pigmentos, como o azul de metileno (MB) a luz e capaz de gerar 1O2, sendo essa a base da terapia fotodin?mica (TFD). Quando em excesso, o 1O2 e outras ERRO mostram efeitos t?xicos e carcinog?nicos e est?o relacionados ao envelhecimento e a etiologia de varias doen?as, incluindo artrite, doen?as degenerativas e c?ncer. A principal via de reparo de danos oxidativos ao DNA e a via por excis?o de base (BER). No entanto, estudos recentes tem observado a atua??o de fatores da via de reparo por excisao de nucleotideo (NER) na corre??o desse tipo de les?o. Um dos fatores da via NER e a prote?na Xeroderma Pigmentoso do Grupo de complementa??o A (XPA), que atua em conjunto com outras prote?nas na etapa de localiza??o dos s?tios de danos e de recrutamento de outros fatores de reparo. Ainda, agentes oxidativos como o 1O2 sao capazes de induzir a express?o g?nica. Nesse contexto, o presente trabalho teve como objetivo principal avaliar a resposta de c?lulas deficientes em XPA ao tratamento com MB fotossensibilizado. Para isso, foram analisadas a viabilidade celular e a ocorr?ncia de danos oxidativos em linhagens proficientes e deficientes em XPA, assim como a express?o dessa enzima em c?lulas proficientes e complementadas. Nossos resultados indicam um aumento da resist?ncia ao tratamento em c?lulas complementadas com XPA e um maior n?vel de danos oxidativos em linhagens deficientes nessa enzima. Alem disso, o tratamento foi respons?vel pela modula??o da express?o dessa enzima ate 24h depois. Esses resultados indicam uma evidencia direta da participa??o de enzimas do NER no reparo de danos oxidativos e contribui para um melhor entendimento sobre os efeitos da TFD na indu??o da express?o g?nica
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Replication-Mediated Disassociation of Replication Protein A-XPA Complex Upon Dna Damage: Implications for RPA Handing OffJiang, Gaofeng, Zou, Yue, Wu, Xiaoming 01 August 2012 (has links)
RPA (replication protein A), the eukaryotic ssDNA (single-stranded DNA)-binding protein, participates in most cellular processes in response to genotoxic insults, such as NER (nucleotide excision repair), DNA, DSB (double-strand break) repair and activation of cell cycle checkpoint signalling. RPA interacts with XPA (xeroderma pigmentosum A) and functions in early stage of NER. We have shown that in cells the RPA-XPA complex disassociated upon exposure of cells to high dose of UV irradiation. The dissociation required replication stress and was partially attributed to tRPA hyperphosphorylation. Treatment of cells with CPT (camptothecin) and HU (hydroxyurea), which cause DSB DNA damage and replication fork collapse respectively and also leads to the disruption of RPA-XPA complex. Purified RPA and XPA were unable to form complex in vitro in the presence of ssDNA. We propose that the competition-based RPA switch among different DNA metabolic pathways regulates the dissociation of RPA with XPA in cells after DNA damage. The biological significances of RPA-XPA complex disruption in relation with checkpoint activation, DSB repair and RPA hyperphosphorylation are discussed.
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New Insights into the Roles of Human DNA Damage Checkpoint Protein ATR in the Regulation of Nucleotide Excision Repair and DNA Damage-Induced Cell DeathLi, Zhengke 01 December 2013 (has links) (PDF)
Integrity of the human genome is frequently threatened by endogenous and exogenous DNA damaging reagents that may lead to genome instability and cancer. Cells have evolved multiple mechanisms to repair DNA damage or to eliminate the damaged cells beyond repair and to prevent diverse diseases. Among these are ataxia telangiectasia and Rad3-related (ATR)-mediated DNA damage checkpoint and nucleotide excision repair (NER) that are the major pathways by which cells handle ultraviolet C (UV-C)- or other exogenous genotoxin-induced bulky DNA damage. However, it is unclear how these 2 pathways may be coordinated. In this study we show that ATR physically interacts with NER factor xeroderma pigmentosum group A (XPA) where an ATR phosphorylation site on serine 196 is located. Phosphorylation of XPA on serine 196 is required for repair of UV-induced DNA damage. In addition, a K188A point mutation of XPA that disrupts the ATR-XPA interaction inhibits the UV-induced XPA phosphorylation and DNA repair. Moreover, we show that depletion of p53, a downstream checkpoint of ATR, and inhibition of p53 transcriptional activities reduced the UV-induced XPA import. Furthermore, we found that the ATR-directed XPA nuclear import happens primarily in the S phase of the cell cycle. In effort to determine the mechanism involved in the XPA nuclear import, we found that, in addition to the nuclear localization signal (NLS) of XPA, importin-α4 is required for the UV-induced XPA nuclear import in an ATR-dependent manner. These data suggest that NER could be regulated by the ATR-dependent checkpoint via modulation of XPA phosphorylation and nuclear import. In a separate study we show that, upon UV damage, cytoplasmic ATR translocates to mitochondria, blocks the recruitment of proapoptotic Bcl-2–associated X (Bax) protein to mitochondria and prevents the loss of mitochondrial membrane potential (ΔΨ) and apoptosis. Bax-depletion reduces the effect of ATR on ΔΨ. Remarkably, the cytoplasmic ATR exhibits no checkpoint kinase activity, a hallmark function of nuclear ATR. Silencing of ATR’s kinase activity failed to affect Bax relocalization to mitochondria. These results reveal a novel checkpoint-independent antiapoptotic function of ATR at mitochondria in the cellular response to DNA damage.
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Structural and Biochemical Investigation of the Molecular Mechanisms of DNA Response and Repair in Humans and <em>Escherichia coli</em>.Shell, Steven Michael 03 May 2008 (has links) (PDF)
The genomes of all living cells are under constant attack from both endogenous and exogenous agents that damage DNA. In order to maintain genetic integrity a variety of response pathways have evolved to recognize and eliminate DNA damage. Replication protein A (RPA), the eukaryotic single-stranded DNA (ssDNA) binding protein, is a required factor for all major DNA metabolisms. Although much work has been done to elucidate the nature of the interaction between RPA and ssDNA currently there is no structural information on how the full-length protein binds to ssDNA. This study presents a novel examination of the full nucleoprotein complex formed between RPA and ssDNA. We identified three previously unknown contacts between ssDNA and lysine residues in DNA binding domain C located in the p70 subunit. This represents the first single amino-acid resolution determination of how full-length RPA contacts ssDNA. The Ataxia-Telangiectasia Mutated and RAD3-Related (ATR) mediated DNA damage checkpoint and nucleotide excision repair (NER) pathway are primarily responsible for repair of UV-C-induced photolesions in DNA. However, it is unclear how these two pathways are coordinated. We found the ATR-dependent checkpoint induces a rapid nuclear accumulation of the required NER factor Xeroderma pigmentosum group A (XPA) in both a dose- and time-dependent fashion. Also, using surface topology mapping we have defined an α-helix motif on XPA required for XPA-ATR complex formation necessary for XPA phosphorylation. In addition, we have determined that XPA phosphorylation promotes repair of persistent DNA lesions, such as cyclobutane pyrimidine dimers. The basis for initial damage recognition in NER is structural distortion of duplex DNA; however, the effects of adduct structure and sequence on strand opening and recognition are unclear. Using the E. coli NER system we determined that the identity of the adduct dictates the size of the strand opening generated by the UvrA2B complex. In addition we found that the sequence immediately surrounding the damaged nucleotide affects damage recognition by influencing the amount of helical distortion induced by the adduct. These effects are a result of the equilibrium conformation the adduct adopts in addition to the amount of hydrogen bonding available to maintain the structure.
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Étude protéomique des partenaires d`interaction de XPA en présence et en absence de dommage à l`ADNSekheri, Meriem S. 01 1900 (has links)
La réparation par excision de nucléotides (NER) permet l'élimination des lésions provoquant une distorsion de la double hélice de l’ADN. Ces lésions sont induites par plusieurs agents environnementaux comme les rayons UV, ainsi que par certaines drogues chimio- thérapeutiques tel que le cisplatine. Des défauts dans la NER conduisent à de rares maladies autosomiques héréditaires : La xérodermie pigmentaire (XP), le syndrome de Cockayne (CS), le syndrome de sensibilité aux UVSS et la trichothiodystrophie (TTD). Ces maladies sont associées soit à une prédisposition élevée au cancer de la peau et / ou à de graves anomalies du développement neurologique. Le groupe de patients XP-A représente le deuxième groupe (XP) le plus fréquent, et possède la forme la plus sévère combinant cancer de la peau avec un haut risque de dégénérescence neurologique. À date, aucune explication n`a été proposée pour les symptômes neurologiques observés chez ces patients. Nous avions suggéré ainsi que la protéine XPA possède d`autres fonctions dans d`autres processus cellulaires, ceci en interagissant avec des partenaires protéiques différents de ceux déjà connus. Afin de confirmer cette hypothèse nous avions réalisé une étude protéomique à grande échelle en combinant la spectrométrie de masse à une immunoprécipitation en Tandem d`affinité (TAP), afin d`identifier de nouvelles protéines interagissant directement avec XPA. Nous avions montré que XPA peut interagir avec MRE11, la protéine clé de la réparation par recombinaison homologue. Des études additionnelles sont requises pour confirmer cette interaction et comprendre sa fonction / To maintain genome integrity and ensure the continuation of transcription, helix distorting DNA lesions induced by UV and other environmental mutagens are eliminated through a highly-versatile DNA repair pathway: nucleotide excision repair (NER). Mutations in 11 genes (XPC, XPE, XPB, XPD, XPG, XPA, XPG, TTD-A, CSA, CSB and UVSSA), among the 30 genes directly involved in NER, have been associated with the human genetic disorders: xeroderma pigmentosum (XP), cockayne syndrome (CS), trichothiodystrophy (TTD), and UV-sensitive syndrome (UVSS). Patients of these syndromes display a wide variety of clinical features that range from normal development with extreme predisposition to cancer, to neurodevelopmental defects associated with premature aging abnormalities. The connection between DNA damage and neurodegeneration remains unclear, i.e. cannot be explained by a DNA-repair deficiency alone, implying that various repair factors perform other functions beyond the repair process. XP-A is the second most common form of XP. XP-A cells have very low levels of NER activity and are sensitive to killing by UV light. It is one of the most severely affected XP groups, with the onset of cutaneous features, skin cancer, ocular features, and severe early onset neurological disease. Therefore we hypothesize that XPA interacts with cellular proteins that regulate its functions either in UV damage repair or in neurological development. To test this, our major aim was to carry out a large-scale proteomics investigation to identify novel interacting partners for XPA in the absence or presence of genotoxic stress, thus providing clues on the origins of neurodegeneration observed in many XP-A patients. We provide evidence that XPA can interact with MRE11, the key factor in repair of double strand breraks by homologous Recombination. Future experiments will be aimed at determining the impact of the XPA/MRE11 interaction functions in cells.
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