Background: The prevalence of immunological disorders such as type 1 diabetes (T1D) is increasingly common amongst children, adolescents and young adults. There is also an increase in psychosomatic symptoms (depression, insomnia, anxiety, headaches and fatigue etc.) as well as a decrease in physical activity amongst young people, affecting the well-being and overall health of our younger population. It is therefore important to study the effects of psychological and physical stressors on the immune system, to evaluate their impact on juvenile health. Aim: This thesis explores the impact of psychological and physical stressors on the cellular immune system with special focus on diabetes-related immunity in a young population, using an interdisciplinary approach. Method: When exploring the impact of psychological and physical stressors such as psychological stress due to exposure to psychological stressful experiences or degree of physical activity/training on the cellular immune system in children, adolescents and young women, peripheral blood mononuclear cells (PBMC) were stimulated with antigens (tetanus toxoid (TT) and β-lactoglobulin (βLG)) as well as diabetes-related autoantigens (insulin, heat shock protein 60 (HSP60), tyrosine phosphatase-2 (IA-2) and glutamic acid decarboxylase 65 (GAD65)) and secreted cytokines and chemokines were measured by multiplex fluorochrome technique (Luminex). Populations of Thelper (Th) cells (CD4+), T-cytotoxic (Tc) cells (CD8+), B cells (CD19+), Natural Killer (NK) cells (CD56+CD16+) as well as regulatory T (Treg) cells (CD4+CD25+FoxP3+CD127-), and their expression of CD39 and CD45RA were studied by flow cytometry. Diabetes-related parameters (glucose, C-peptide,proinsulin, pancreatic polypeptide and peptide YY) were measured to studyβ-cell activity and appetite regulation and cortisol was used as a biological marker for psychological and physical stress. Results: Children in families exposed to psychological stress showed an imbalanced cellular immune response as well as an increased immune response towards diabetes-related autoantigens. Also, previous exposure to psychological stress as well as current exposure to psychological stress in young women showed an increased immune response towards diabetes-related autoantigens. Further, previous exposure to psychological stress in young women showed increased numbers of circulating CD56+CD16+ NK cells as wellas decreased numbers of circulating CD4+CD25+FoxP3+CD127- Treg cells. High physical activity in children showed decreased spontaneous immune response as well as a decreased immune response towards diabetes-related autoantigens, while low physical activity in children showed an increased immune response towards diabetes-related autoantigens. Further, endurance training in adolescents, especially in adolescent males and young adolescents, showed an increased immune response towards the diabetes-related autoantigen IA-2. Conclusion: It is evident that psychological and physical stressors such as exposure to psychological stress and degree of physical activity/training impact the cellular immune system. Experiences associated with psychological stress seem to have a negative effect on the cellular immune system in a young population, causing an imbalance in the immune system that could possibly induce diabetes-related immunity. High physical activity in children seems to have a protective effect against diabetes-related immunity. In contrast, low physical activity in children and endurance training in adolescents seems to induce diabetes-related immunity. It is very likely that psychological stressful experiences, low physical activity and intense training such as endurance training all play important roles in the immunological process leading to the development of type 1 diabetes.

Orientador: Salvador Antonio Mireles Sandoval / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Educação / Made available in DSpace on 2018-08-05T03:52:27Z (GMT). No. of bitstreams: 1 Candido_MariaCristinaMachado_M.pdf: 12028541 bytes, checksum: 70ee33447773d71f0e3fe70f26cfc6c5 (MD5) Previous issue date: 2005 / Resumo: O objetivo do presente estudo é desenvolver através de uma pesquisa documental, por meio de relatórios e dados do Projeto Convivência e Cidadania e do Programa de Erradicação do Trabalho Infantil (PETI), crianças, adolescentes e respectivas famílias, visando atualizar dados e redefinir o perfil das crianças/adolescentes que ainda trabalham nas ruas de Campinas. A hipótese preliminar que orientou a presente pesquisa consiste na seguinte afirmação: ¿Graças à atuação do PETI e outros programas, o número de crianças, gradativamente, está diminuindo e alterando o perfil das crianças que ainda continuam trabalhando nas ruas e respectivas famílias¿. A expectativa é de que um dia, num futuro próximo, a curto ou médio prazo, as pesquisas revelarão a inexistência de crianças trabalhando nas ruas de Campinas. A justificativa para a realização deste estudo apóia-se na triste realidade do trabalho infanto-juvenil que a sociedade em geral luta para erradicar sem muito sucesso. A limitação do tema aos resultados das ações do PETI no município de Campinas funda-se na importância de se obter um retrato atualizado desse trabalho entre a população infanto-juvenil em uma das cidades mais desenvolvidas do país, nos aspectos sócio-cultural e econômico. A metodologia do presente estudo orienta-se pela técnica de análise documental, que mais adequadamente atende aos objetivos propostos. O procedimento teve início com a autorização para a pesquisa e esta prossegue com a análise dos relatórios do Projeto Convivência e Cidadania, do Projeto de Erradicação do Trabalho Infantil (PETI) e do Mapa de vulnerabilidade Social (MVS) fornecidos pela Secretaria de Assistência Social da Prefeitura Municipal de Campinas. A presente dissertação faz um resgate histórico sobre o trabalho infantil, apresentando uma abordagem das transformações sócio-econômicas até os dias atuais, o surgimento de no município de Campinas, além dos dados de abordagens e acompanhamento do Projeto Convivência e Cidadania e do PETI. Os procedimentos metodológicos baseiam-se nos relatórios do PETI fornecido pela Prefeitura Municipal de Campinas e colaboração dos executores das ações junto à população infanto-juvenil assistida e respectivas famílias, sobre as ações e resultados do Programa durante o ano de 2004. A seguir, apresenta-se uma análise dos dados obtidos através da pesquisa documental, com destaque para um estudo sobre as crianças que trabalham nas ruas de Campinas organizações internacionais com o objetivo de defender os direitos das crianças, legislação de proteção a esses direitos e implementação de programas de combate e erradicação do trabalho infantil. Também apresenta um perfil da cidade de Campinas segundo o MVS e descreve as características do PETI federal, procurando destacar as mudanças que sofreu em sua implementação e respectivas famílias e como são atendidas pelo PETI. Concluímos que o perfil das crianças/adolescentes que têm sido encontradas pelas ruas e respectivas famílias continuam apresentando as mesmas características, porque, em sua maioria, são oriundas das mesmas regiões de onde vieram as anteriores. As que já foram ou estão sendo atendidas pelo PETI, já não são encontradas pelas ruas, pois já estão sendo orientadas e recebendo ajuda. As que estão hoje pelas ruas vendendo balas já pertencem a novos contingentes de migrantes, a espera de ações dos programas sociais. Conclui-se, então, que será necessário expandir as ações do PETI, visando fazer com que o número de beneficiados supere o número crescente de crianças/adolescentes e respectivas famílias que ainda esperam ter acesso aos benefícios sociais / Abstract: The objective of the present study is to develop a together research the children, adolescent and respective families, aiming at to bring up to date given and to redefine the profile of the crianças/adolescentes that still work in the streets of Campinas. The preliminary hypothesis that guided the present research consists of the following affirmation: "Thanks to the performance of the PETI and other programs, the number of children, gradual, it is diminishing and modifying the profile of the children who still continue working in the streets and respective families. The expectation is of that one day, in a next future, the short or average stated period, the research will disclose the inexistence of children working in the streets of Campinas. The justification for the accomplishment of this apóia study in the sad reality of the infanto-youthful work that the society in general fight to eradicate without much success. The limitation of the subject to the results of the actions of the PETI in the city of Campinas is established in the importance of if to get a brought up to date picture of this work more enters the infanto-youthful population in one of the developed cities of the parents, in the aspects partner-cultural and economic. The methodology is oriented for the qualitative boarding, through the documentary technique of analysis. The procedure had beginning with the authorization for the research and this continues with the analysis of the reports of the Project Convivência and Cidadania and the Project of Eradication of Trabalho Infantil (PETI) supplied by the Secretariat of Social Assistance of the Municipal City hall of Campinas. The present dissertação makes a historical rescue on the infantile work, presenting a boarding of the partner-economic transformations until the current days, the sprouting of international organizations with the objective to defend the rights of the children, legislation of protection to these rights and implementation of programs of combat and eradication of the infantile work. Also it presents a profile of the city of Campinas and describes the characteristics of the federal PETI, looking for to detach the changes that suffered in its implementation in the city of Campinas, beyond the data boardings and accompaniment the Project Convivência and Cidadania and the PETI. The metodológicos procedures are based on the reports of the PETI supplied for the Municipal City hall of Campinas and contribution of the executors of the together actions to the infanto-youthful population attended and respective families, on the actions and results of the Program during the year of 2004. To follow, an analysis of the data gotten through the documentary research is presented, with prominence for a study on the children who work in the streets of Campinas and respective families and as they are taken care of by the PETI. We conclude that the profile of the crianças/adolescentes that have been found for the streets and respective families continues presenting the same characteristics, because, in its majority, they are deriving of the same regions of where they had come the previous ones. The ones that already had been or are being taken care of for the PETI, already are not found by the streets, therefore already they are being guided and receiving aid. The ones that they are today for the streets vendendo bullets already belong the new contingents of migrantes, the wait of action of the social programs. One concludes, then, that it will be necessary to expand the actions of the PETI, aiming at to make with that the number of benefited surpasses the increasing number of crianças/adolescentes and respective families who still wait to have access to the social benefits / Mestrado / Educação, Sociedade, Politica e Cultura / Mestre em Educação

Title: The effect of one-off so called sensorimotor serie exercise on postural stability measured on Neurocom SMART Equitest Aim: The goal of this thesis is to evaluate the effect of one-off so-called sensorimotor serie exercise on postural stability on a healthy young human measured by computerized dynamic posturography (CDP). Methods: This experimental study is using a cross-over design, it means that participants got the intervention in randomized order. 20 healthy young subjects participated in the experiment, 12 women and 8 men aged 20 to 28 years (23,55± 1,63). The group of men and women was randomly divided into two groups. Group E1 was measured by Neurocom SMART Equitest using three testing protocols (SOT, MCT, LOS), went through the one-off so called sensorimotor serie, and was measured again immediately afterwards (almost 30 minutes after the first measurement) at the first appointment. In 1 week ± 1 day (wash-out period) participants from the group E1 were only measured without getting any intervention and after 30 minutes break measured again. Group E2 got the intervention in opposite order. Measured data were processed using the Neurocom Balance Manager Software. In the statistical analysis was used one-sample t-test respectively two-sample t-test (Welch's version) as a comparison...

The development of a mega project in relation with its local proximity is a major challenge in urban planning. This study explores young residents’ perceptions of Paris-Saclay, France, an academic mega cluster in the south of Paris. The intent is to understand the role an academic mega project can play in the reduction of social inequalities at a local scale, among young residents. Research about mega projects and academic clusters in relation to the local environment is a growing research field in planning research. To explore young residents’ perceptions is however an underexplored issue, but of high relevance considering that young French people face growing inequalities reflected in the urban fabric. An empirical study has been carried out to explore young people’s perceptions and attitudes towards the project by conducting focus group discussions in high schools inside and nearby the area of the academic cluster. The results show that the geographical proximity does not influence the respondents’ perceived connection to the academic cluster to a greater extent, rather, it is the respondents’ personal social relations that are the key point to create a sense of connection to the academic cluster. Future planning actions may be oriented towards improving the communication of the project and to offer various networks between the academic cluster and the young population. Such initiatives will not "solve" social segregation but are seen as measures to improve the situation for young residents within the planner's responsibilities.

Dados sobre a avaliação não invasiva da rigidez vascular e suas relações com variáveis de risco cardiovascular são escassos em jovens. Objetiva avaliar a relação entre a velocidade de onda de pulso (VOP) e a pressão arterial (PA), variáveis antropométricas, metabólicas, inflamatórias e de disfunção endotelial em indivíduos adultos jovens. Foram estudados 96 indivíduos (51 homens) do Estudo do Rio de Janeiro, em duas avaliações, A1 e A2, com intervalo de 17,691,58 anos (16 a 21 anos). Em A1 foram avaliados em suas escolas (10-15 anos - média 12,421,47 anos) e em A2 foram novamente avaliados em nível ambulatorial (26-35 anos - média 30,091,92 anos). Em A1 foram obtidos pressão arterial (PA) e índice de massa corporal (IMC). Em A2 foram obtidos a velocidade da onda de pulso (VOP)-método Complior, PA, IMC, circunferência abdominal (CA), glicose, perfil lipídico, leptina, insulina, adiponectina, o índice de resistência à insulina HOMA-IR, proteína C-Reativa ultrassensível (PCRus) e as moléculas de adesão E-selectina, Vascular Cell Adhesion Molecule-1(VCAM-1) e Intercellular Adhesion Molecule-1 (ICAM-1). Foram obtidos, ainda, a variação da PA e do IMC entre as 2 avaliações. Em A2 os indivíduos foram estratificados segundo o tercil da VOP para cada sexo. Como resultados temos: 1) Os grupos foram constituídos da seguinte forma: Tercil 1:homens com VOP < 8,69 m/s e mulheres com VOP < 7,66 m/s; Tercil 2: homens com VOP &#8805; 8,69 m/s e < 9,65m/s e mulheres com VOP &#8805; 7,66 m/s e < 8,31m/s;Tercil 3:homens com VOP &#8805; 9,65 m/s e mulheres com VOP &#8805; 8,31 m/s. 2) O grupo com maior tercil de VOP mostrou maiores médias de PA sistólica (PAS) (p=0,005), PA diastólica (PAD) (p=0,007), PA média (PAM) (p=0,004), variação da PAD (p=0,032), variação da PAM (p=0,003), IMC (p=0,046), variação do IMC (p=0,020), insulina (p=0,019), HOMA-IR (p=0,021), E-selectina (p=0,032) e menores médias de adiponectina (p=0,016), além de maiores prevalências de diabetes mellitus/intolerância à glicose (p=0,022) e hiperinsulinemia (p=0,038); 3) Houve correlação significativa e positiva da VOP com PAS (p<0,001), PAD (p<0,001), PP (p=0,048) e PAM (p<0,001) de A2, com a variação da pressão arterial (PAS, PAD e PAM) (p<0,001) entre as duas avaliações, com o IMC de A2 (p=0,005) e com a variação do IMC (p<0,001) entre as duas avaliações, com CA (p=0,001), LDLcolesterol (p=0,049) e E-selectina (p<0,001) e correlação negativa com HDLcolesterol (p<0,001) e adiponectina (p<0,001); 4)Em modelo de regressão múltipla, após ajuste do HDL-colesterol, LDLcolesterol e adiponectina para sexo, idade, IMC e PAM, apenas o sexo masculino e a PAM mantiveram correlação significativa com a VOP. A VOP em adultos jovens mostrou relação significativa com variáveis de risco cardiovascular, destacando-se o sexo masculino e a PAM como importantes variáveis no seu determinismo. Os achados sugerem que a medida da VOP pode ser útil para a identificação do acometimento vascular nessa faixa etária. / Data on non-invasive evaluation of vascular stiffness in the young and its relationship with cardiovascular (CV) risk variables are scarce. Objective to assess the relationship between pulse wave velocity (PWV) and blood pressure (BP), anthropometric, metabolic, inflammatory and endothelial dysfunction variables in young adults. Ninety-six individuals (51 males) from The Rio de Janeiro Study cohort were studied in two evaluations, A1 and A2, with an interval of 17.69 1.58 years (16-21 years). In A 1 they were evaluated at their schools (10-15 years average 12.42 1.47 years) and in A2 they were all re-evaluated as outpatients (26-35 years - average 30.09 1.92 years). In A1 BP and body mass index (BMI) were obtained. In A2 pulse wave velocity (PWV) by Complior method, BP, BMI, waist circumference (WC), glucose, lipid profile, leptin, insulin, adiponectin, the HOMA-IR insulin resistance index, high sensitive C-Reactive protein (CRPhs) and E-selectin, Vascular Cell Adhesion Molecule 1 (VCAM-1) and Intercellular Adhesion Molecule 1 (ICAM-1) adhesion molecules were obtained. The BP and BMI variation over the time interval between the two evaluations were also obtained. Subjects were stratified according to tertile of PWV for each sex in A2. As results: 1) The groups were constituted as follows: Tertile 1: males with PWV <8.69 m/s and females with PWV <7.66 m/s; Tertile 2: males with PWV &#8805; 8.69 m/s and <9.65 m/s and females with PWV &#8805; 7.66 m/s and <8.31 m/s; Tertile 3: males with PWV &#8805; 9.65 m/s and females with PWV &#8805; 8.31 m/s 2) The group with the highest PWV tertile showed higher values of systolic BP (SBP) (p=0.005), diastolic BP (DBP) (p=0.007), mean BP (MBP) (p=0,004), DBP variation (p=0,032), MBP variation (p=0.033), BMI (p=0.046), BMI variation (p=0.020), insulin (p=0.019), HOMA-IR (p=0.021), E-Selectin (p=0.032) and lower values of adiponectin (p=0.016), besides higher prevalence of diabetes mellitus/glucose intolerance (p=0.022) and hyperinsulinemia (p=0.038); 3) There were a significant positive correlation of PWV with SBP (p<0,001), DBP (p<0,001), PP (p=0,048) and MBP (p<0,001) from A2, variation in blood pressure (SBP, DBP, and MBP) (p<0,001) between the two assessments, BMI (p=0.005) and BMI variation between the two evaluations (p<0,001), WC (p=0.001), LDL-cholesterol (0.049), and E-selectin (p<0,001) and negative correlation with HDL-cholesterol (p<0,001) and adiponectin (p<0,001); 4) In the multiple regression model, HDL-cholesterol, LDL-cholesterol and adiponectin lost statistical significance after adjustment for sex, age, BMI and MBP, only the male gender and MBP remained significantly correlated with PWV. PWV in young adults showed a significant association with CV risk variables, highlighting the male gender and MBP as important variables in its determining. The findings suggest that measurement of PWV can be useful for the identification of vascular impairment in this age group.

Dados sobre a avaliação não invasiva da rigidez vascular e suas relações com variáveis de risco cardiovascular são escassos em jovens. Objetiva avaliar a relação entre a velocidade de onda de pulso (VOP) e a pressão arterial (PA), variáveis antropométricas, metabólicas, inflamatórias e de disfunção endotelial em indivíduos adultos jovens. Foram estudados 96 indivíduos (51 homens) do Estudo do Rio de Janeiro, em duas avaliações, A1 e A2, com intervalo de 17,691,58 anos (16 a 21 anos). Em A1 foram avaliados em suas escolas (10-15 anos - média 12,421,47 anos) e em A2 foram novamente avaliados em nível ambulatorial (26-35 anos - média 30,091,92 anos). Em A1 foram obtidos pressão arterial (PA) e índice de massa corporal (IMC). Em A2 foram obtidos a velocidade da onda de pulso (VOP)-método Complior, PA, IMC, circunferência abdominal (CA), glicose, perfil lipídico, leptina, insulina, adiponectina, o índice de resistência à insulina HOMA-IR, proteína C-Reativa ultrassensível (PCRus) e as moléculas de adesão E-selectina, Vascular Cell Adhesion Molecule-1(VCAM-1) e Intercellular Adhesion Molecule-1 (ICAM-1). Foram obtidos, ainda, a variação da PA e do IMC entre as 2 avaliações. Em A2 os indivíduos foram estratificados segundo o tercil da VOP para cada sexo. Como resultados temos: 1) Os grupos foram constituídos da seguinte forma: Tercil 1:homens com VOP < 8,69 m/s e mulheres com VOP < 7,66 m/s; Tercil 2: homens com VOP &#8805; 8,69 m/s e < 9,65m/s e mulheres com VOP &#8805; 7,66 m/s e < 8,31m/s;Tercil 3:homens com VOP &#8805; 9,65 m/s e mulheres com VOP &#8805; 8,31 m/s. 2) O grupo com maior tercil de VOP mostrou maiores médias de PA sistólica (PAS) (p=0,005), PA diastólica (PAD) (p=0,007), PA média (PAM) (p=0,004), variação da PAD (p=0,032), variação da PAM (p=0,003), IMC (p=0,046), variação do IMC (p=0,020), insulina (p=0,019), HOMA-IR (p=0,021), E-selectina (p=0,032) e menores médias de adiponectina (p=0,016), além de maiores prevalências de diabetes mellitus/intolerância à glicose (p=0,022) e hiperinsulinemia (p=0,038); 3) Houve correlação significativa e positiva da VOP com PAS (p<0,001), PAD (p<0,001), PP (p=0,048) e PAM (p<0,001) de A2, com a variação da pressão arterial (PAS, PAD e PAM) (p<0,001) entre as duas avaliações, com o IMC de A2 (p=0,005) e com a variação do IMC (p<0,001) entre as duas avaliações, com CA (p=0,001), LDLcolesterol (p=0,049) e E-selectina (p<0,001) e correlação negativa com HDLcolesterol (p<0,001) e adiponectina (p<0,001); 4)Em modelo de regressão múltipla, após ajuste do HDL-colesterol, LDLcolesterol e adiponectina para sexo, idade, IMC e PAM, apenas o sexo masculino e a PAM mantiveram correlação significativa com a VOP. A VOP em adultos jovens mostrou relação significativa com variáveis de risco cardiovascular, destacando-se o sexo masculino e a PAM como importantes variáveis no seu determinismo. Os achados sugerem que a medida da VOP pode ser útil para a identificação do acometimento vascular nessa faixa etária. / Data on non-invasive evaluation of vascular stiffness in the young and its relationship with cardiovascular (CV) risk variables are scarce. Objective to assess the relationship between pulse wave velocity (PWV) and blood pressure (BP), anthropometric, metabolic, inflammatory and endothelial dysfunction variables in young adults. Ninety-six individuals (51 males) from The Rio de Janeiro Study cohort were studied in two evaluations, A1 and A2, with an interval of 17.69 1.58 years (16-21 years). In A 1 they were evaluated at their schools (10-15 years average 12.42 1.47 years) and in A2 they were all re-evaluated as outpatients (26-35 years - average 30.09 1.92 years). In A1 BP and body mass index (BMI) were obtained. In A2 pulse wave velocity (PWV) by Complior method, BP, BMI, waist circumference (WC), glucose, lipid profile, leptin, insulin, adiponectin, the HOMA-IR insulin resistance index, high sensitive C-Reactive protein (CRPhs) and E-selectin, Vascular Cell Adhesion Molecule 1 (VCAM-1) and Intercellular Adhesion Molecule 1 (ICAM-1) adhesion molecules were obtained. The BP and BMI variation over the time interval between the two evaluations were also obtained. Subjects were stratified according to tertile of PWV for each sex in A2. As results: 1) The groups were constituted as follows: Tertile 1: males with PWV <8.69 m/s and females with PWV <7.66 m/s; Tertile 2: males with PWV &#8805; 8.69 m/s and <9.65 m/s and females with PWV &#8805; 7.66 m/s and <8.31 m/s; Tertile 3: males with PWV &#8805; 9.65 m/s and females with PWV &#8805; 8.31 m/s 2) The group with the highest PWV tertile showed higher values of systolic BP (SBP) (p=0.005), diastolic BP (DBP) (p=0.007), mean BP (MBP) (p=0,004), DBP variation (p=0,032), MBP variation (p=0.033), BMI (p=0.046), BMI variation (p=0.020), insulin (p=0.019), HOMA-IR (p=0.021), E-Selectin (p=0.032) and lower values of adiponectin (p=0.016), besides higher prevalence of diabetes mellitus/glucose intolerance (p=0.022) and hyperinsulinemia (p=0.038); 3) There were a significant positive correlation of PWV with SBP (p<0,001), DBP (p<0,001), PP (p=0,048) and MBP (p<0,001) from A2, variation in blood pressure (SBP, DBP, and MBP) (p<0,001) between the two assessments, BMI (p=0.005) and BMI variation between the two evaluations (p<0,001), WC (p=0.001), LDL-cholesterol (0.049), and E-selectin (p<0,001) and negative correlation with HDL-cholesterol (p<0,001) and adiponectin (p<0,001); 4) In the multiple regression model, HDL-cholesterol, LDL-cholesterol and adiponectin lost statistical significance after adjustment for sex, age, BMI and MBP, only the male gender and MBP remained significantly correlated with PWV. PWV in young adults showed a significant association with CV risk variables, highlighting the male gender and MBP as important variables in its determining. The findings suggest that measurement of PWV can be useful for the identification of vascular impairment in this age group.