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Previous issue date: 2016-10-31 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Acute phase proteins are glycoproteins produced by hepatocytes and released into the bloodstream in response to tissue damage resulting from acute inflammatory processes. Currently, they are considered biomarkers of inflammation or infection, compared with other variables such as hyperthermia, leukocytosis and neutrophilia, but in donkeys have not yet been analyzed. This study aimed to evaluate acute inflammatory response in donkeys submitted to ovariectomy by two different approaches. We randomly selected 18 adult females animals weighing on average 100 kg, which were divided into two groups: Group I, 9 animals were ovariectomized by laparotomy and group II, 9 animals by laparoscopy. After the surgeries were evaluated the following variables in whole blood: blood count, white blood cell count differential cell count, fibrinogen, haptoglobin, albumin, antitrypsin, alpha-1-acid glycoprotein, ceruloplasmin, transferrin, immunoglobulins A and G (acute phase proteins) . In the same time, the peritoneal fluid were measured: number of erythrocytes and leukocytes, cytological analysis differential (leukocytes and mesothelial cells), total protein, pH and acute phase proteins. All variables were analyzed before surgery, 12, 24, 48, 72 hours, 8 and 16 days after surgery. There was an increase (p <0.05) in the number of leukocytes with neutrophilia after surgery, regardless of the surgical serum and peritoneal procedure. Acute phase proteins had different behaviors in blood and peritoneal fluid. For the first time, we identified a P23.000kD protein in serum and peritoneal samples in donkeys. The interest on the role of acute phase proteins in the veterinary medicine, particularly in horses is growing, mainly aiming to use them as early markers of inflammation / Proteínas de fase aguda são glicoproteínas produzidas pelos hepatócitos e liberadas na corrente sanguínea em resposta a dano tecidual, decorrente de processos inflamatórios agudos. Atualmente, são consideradas biomarcadores da resposta inflamatória e infecciosa, quando comparada com outras variáveis como hipertermia, leucocitose e neutrofilia, porém em asininos ainda não foram bem analisadas. Objetivou-se avaliar resposta inflamatória aguda em asininos submetidos à ovariectomia por duas diferentes abordagens. Foram utilizados 18 animais, fêmeas adultas, pesando em média 100 kg, as quais foram divididas em dois grupos, de 09 animais cada, onde grupo I, foram ovariectomizadas por laparotomia e o grupo II por laparoscopia. Após as cirurgias foram avaliadas as seguintes variáveis no sangue total: hemograma, fibrinogênio, haptoglobina, albumina, antitripsina, alfa-1 glicoproteína ácida, ceruloplasmina, transferrina, imunoglobulinas A e G. Nos mesmos momentos, no líquido peritoneal, foram mensuradas: número de hemácias e leucócitos, análise citológica diferencial (leucócitos e células mesoteliais), proteínas totais, pH e proteínas de fase aguda. Todas as variáveis foram analisadas antes das cirurgias, 12, 24, 48, 72 horas, 8 e 16 dias após os procedimentos cirúrgicos. Houve um aumento (p<0,05) do número de leucócitos com neutrofilia após as cirurgias, independente do procedimento cirúrgico, no soro e líquido peritoneal. As proteínas de fase aguda tiveram comportamentos diferentes no sangue e líquido peritoneal. Neste trabalho identificamos, pela primeira vez, a proteína P23.000kD no soro e amostras peritoneais de asininos. O interesse sobre o papel das proteínas de fase aguda na medicina veterinária, em especial em equídeos vem crescendo, principalmente, visando utilizá-las como marcadores precoces do processo inflamatório / 2017-04-26
| Identifer | oai:union.ndltd.org:IBICT/oai:bdtd.ufersa.edu.br:tede/677 |
| Date | 31 October 2016 |
| Creators | Barros, Isabella de Oliveira |
| Contributors | http://lattes.cnpq.br/0516971232838494, Barrêto Júnior, Raimundo Alves, Silva, Luis Cláudio Lopes Correia da, Nogueira, Andressa Francisca Silva, Paula, Valéria Veras de, Macedo, Michelly Fernandes de, Costa, Wirton Peixoto, Bezerra, Marcelo Barbosa |
| Publisher | Universidade Federal Rural do Semi-Árido, Programa de Pós-Graduação em Ciência Animal, UFERSA, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFERSA, instname:Universidade Federal Rural do Semi-Árido, instacron:UFERSA |
| Rights | info:eu-repo/semantics/openAccess |
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