Made available in DSpace on 2015-03-03T15:38:42Z (GMT). No. of bitstreams: 1
BrunaRMS_TESE.pdf: 1789056 bytes, checksum: 3971348cc480ce2ec7b8b377a649483e (MD5)
Previous issue date: 2011-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Periodontal disease is an inflammatory condition of infectious nature
characterized by destruction of protecting and supporting dental tissues. It happens as a
response produced by the host when attacked by microorganisms. Several factors are
involved in this process. Among them, cytokines are key regulatory molecules in this
immune response, playing a role either protective and/or destructive in lesion
progression. Thus, this study investigated the immunohistochemical expression of IFN-
, GATA-3, IL-17, IL-23, IL-6 and TGF- in gingival tissues of humans, in an attempt
to gain a better understanding of the participation of Th1, Th2 and Th17 immune
responses in the development of periodontal disease processes. To this end, eighty-two
samples of gingival tissues were divided into three groups: Group 1 = 15 (samples of
healthy gum tissue as controls), Group 2 = 36 (samples with chronic gingivitis) and
Group 3 = 31 (samples with chronic periodontitis). All cases were submitted to
morphological analysis from sections stained with hematoxylin and eosin and then
subjected to staining by immunohistochemistry using the streptavidin-biotin method.
Results showed positive labeling for all proteins. Nonetheless, we observed a greater
expression of Th1 cytokines and Th17 cells in group 3. We found statistically
significant difference between TGF- expression and the clinical condition of the
samples (p=0.02). We conclude that Th1 and Th17 responses may act synergistically in
the destructive process of periodontal tissue, overlapping the Th2 response that was also
present in these tissues / A doen?a periodontal ? uma condi??o inflamat?ria de car?ter infeccioso,
caracterizada pela destrui??o dos tecidos de prote??o e sustenta??o dent?rios, face ?
resposta produzida pelo hospedeiro frente ?s agress?es sofridas pelos microrganismos.
V?rios fatores est?o envolvidos nesse processo, sendo as citocinas as principais
mol?culas reguladoras dessa resposta imune, desempenhando um papel protetor e/ou
destrutivo na progress?o da les?o. Diante disso, este experimento investigou a express?o
imuno-histoqu?mica de IFN- , GATA-3, IL-17, IL-23, IL-6 e TGF- em tecidos
gengivais de humanos, na tentativa de se obter um maior entendimento da participa??o
das respostas imunes Th1, Th2 e Th17 no desenvolvimento destes processos
patol?gicos. Para tanto, oitenta e duas amostras de tecidos gengivais foram subdivididas
em tr?s grupos: Grupo 1=15 (amostras de tecido gengival saud?vel-controle), Grupo
2=36 (amostras com gengivite cr?nica) e Grupo 3=31 (amostras com periodontite
cr?nica). Todos os casos foram submetidos ? an?lise morfol?gica a partir de cortes
corados em hematoxilina e eosina e, posteriormente, submetidas ? t?cnica de colora??o
pela imuno-histoqu?mica atrav?s do m?todo da Estreptoavidina-Biotina. Os resultados
mostraram positividade de marca??o para todas as prote?nas, sendo observada uma
maior tend?ncia de marca??o para as citocinas das respostas Th1 e Th17 no grupo 3.
Diferen?a estatisticamente significativa foi verificada entre a express?o de TGF- e a
condi??o cl?nica das amostras (p=0,02). Assim, podemos concluir que as respostas Th1
e Th17 podem atuar sinergicamente no processo destrutivo dos tecidos periodontais,
sobrepondo-se ? resposta Th2 que tamb?m se encontrou presente nestes tecidos
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/18670 |
| Date | 17 February 2011 |
| Creators | Santos, Bruna Rafaela Martins dos |
| Contributors | CPF:28444361453, http://lattes.cnpq.br/9512014003639405, Galv?o, H?bel Cavalcanti, CPF:41291824472, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794937Z9, Miguel, M?rcia Cristina da Costa, CPF:82134731400, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707501Z3&dataRevisao=null, Barros, Simone Souza Le?o Veras, CPF:39783391372, http://lattes.cnpq.br/8508847080125024, Lemos, Jana?na Cavalcante, CPF:77846532472, http://lattes.cnpq.br/6756263467790240, Freitas, Roseana de Almeida |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0024 seconds